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Biomedical subjects

T Irie

Publications and source records attributed to T Irie.

At least 217 records · Page 12Linked to original sources
PubMed1987

Photoactivation mechanism of hyaluronidase by chlorpromazine.

A pre-irradiated chlorpromazine (CPZ) in pH 7.2 and aqueous solutions activated hyaluronidase, and it showed the enzyme-activating action even after 7 days of irradiation. This indicates that the stable photoproducts of CPZ activated the enzyme. But promazine and CPZ-5-sulfoxide, which are photoproducts of CPZ, did not activate the enzyme in the presence and absence of UVA. The dimer and higher molecular weight (MW) products of CPZ that were obtained by treating the pre-irradiated CPZ solution with gel permeation chromatography showed the hyaluronidase by activating action without UVA. In addition, photoactivation of hyaluronidase by CPZ was inhibited by radical quenchers. This suggested that the photoproducts with enzyme-activating action were produced via some radicals. On the basis of these findings, a possible mechanism for photoactivation of hyaluronidase by CPZ was proposed.

Animals↗
PubMed1987

[Clinical significance of sialic acid determinations in patients with gynecologic cancer].

Sialic acid (SA) levels were measured in human sera by the enzymatic procedure using neuraminidase. Sera were obtained from healthy controls (70 females), patients with uterine myoma (27 cases), cervical cancer (53 cases), benign ovarian tumor (36 cases) and ovarian cancer (70 cases). The upper normal limit of 72mg/dl was obtained from the mean +2SD for healthy controls. The mean value for serum SA in patients with primary ovarian cancer (71.2 +/- 20.6mg/dl) was statistically higher than those for both uterine myomas (59.0 +/- 12.4mg/dl) and controls. The mean level for SA in patients with ovarian cancer was further elevated and it was statistically higher than that for benign ovarian tumors (58.8 +/- 14.3mg/dl), uterine myomas and cervical cancers. SA levels were not well correlated with tumor histologic types or clinical stages of cancer patients. As to the relationship between SA and the clinical course of cancer patients, changes in SA values were well correlated with the curability of tumor resection. The lack of specificity, however, will require careful interpretation of the SA value to assure that inflammation is not causing changes in the test values, because SA shows a transient increase due to the inflammatory reaction during the postoperative period even in patients with benign tumor. These results indicate that SA determination is highly recommended as an addition to the conventional diagnostic methods.

Adult↗
PubMed1986

[Automated fetal heart rate analysis and its trendgram in relation to gas analysis and acid-base balance of umbilical cord arterial blood].

The gas analysis and acid-base balance of the umbilical cord arterial blood of 108 newborn infants were compared with the results of the automated analysis of the intrapartum fetal heart rate (FHR) and its trendgrams. The parameters of the automated analysis of the FHR, which consisted of fetal distress (FD) index, uterine contraction area, FHR score, number of dips, baseline heart rate, variability amplitude and lag time, were shown on the trendgram every 5 minutes using a microcomputer. 1) The mean pH, BE and HCO3- of the 4 groups, that showed 0, 1, 2 and 3 or more on the FD index, were gradually decreased as the FD index was increased. 2) The FHR score, that was accumulated for 10 minutes from 0-10 minutes to 50-60 minutes prior to the birth, was well correlated with the umbilical cord arterial blood pH (r = -0.47), BE (r = -0.60) and HCO3- (r = -0.45). 3) The intrapartum trendgrams were classified into the normal and ominous groups on the basis of the FHR score and FD index. The differences between the respective mean pH, BE, HCO3-, PO2 and PCO2 for these groups were highly significant. 4) The specificity and the sensitivity of the trendgram were more than 70% in the prediction of a pH value lower than 7.25 and of a BE value lower than -6.0 mEq/l.

Acid-Base Equilibrium↗
PubMed1986

[Significance of CA 125 antigen levels in patients with ovarian cancer].

CA 125 antigen levels were measured in patients with ovarian cancer (54 cases) by the RIA method using a monoclonal antibody OC 125 and were examined as a marker for ovarian cancer. The upper normal limit of CA 125 of 35 U/ml was derived from the mean value (15.7 U/ml) + 2 SD (9.3 U/ml) of CA 125 in healthy controls. The mean value of CA 125 in patients with ovarian cancer (1160 +/- 1850 U/ml) was statistically (p less than 0.001) higher than those of healthy controls, benign ovarian tumors (28 +/- 20 U/ml) and cervical cancers (226 +/- 526 U/ml). Elevated CA 125 levels were also found in the early stages pregnancy and endometriosis, but these cases did not show such high CA 125 values as those of ovarian cancers. In addition, CA 125 levels were not affected by the menstrual cycle. Among ovarian malignancies, elevated CA 125 values were specifically demonstrated in serous cystadenocarcinoma (positivity 89%) and markedly low in mucinous cystadenocarcinoma (positivity 16%). No positive correlation of CA 125 values with clinical stage (FIGO) were found in any ovarian cancer patients. The rise or fall of CA 125 level was well correlated with the progression or regression observed in cancer patients with positive CA 125 levels. In conclusion, serum CA 125 determinations may be useful in patients with ovarian cancer (except for mucinous type) for diagnosis and for monitoring the results of treatment.

Antigens, Neoplasm↗
PubMed1986

[Consecutive intra-arterial infusion of cis-diamminedichloroplatinum with continuous intra-arterial infusion of 5-fluorouracil in locally advanced ovarian cancer].

Consecutive intraarterial infusion of cis-diamminedichloroplatinum (CDDP) with continuous intraarterial infusion of 5-fluorouracil (5-FU) (250 mg/day) was administered to 10 patients with locally advanced (peritonitis carcinomatosa) ovarian cancer. The dose of CDDP was 10 mg/5 min/body/day and it was administered for about 10-20 consecutive days per one course. Each course was repeated at about 3-week intervals. The main dose-limiting factor was the number of peripheral leucocytes. The results were 6 PR, 1 MR and 2 NC out of 9 evaluable cases, all of whom had had previous therapy. The antitumor effect needed over 200 mg of intraarterial CDDP and the response lasted from 2 to more than 9 months. Among 6 patients who obtained PR, 4 had failed to respond to intravenous or intraperitoneal infusion of CDDP, which suggested that a high concentration and a critical drug gradient across the tumor cell membrane were more essential for a significant tumor cell kill, with CDDP, than the time of exposure. The toxic effects of intraarterial infusion of CDDP were almost absent as compared with those of intravenous infusion, which consisted of nausea and vomiting. Renal dysfunction was also mild. Other toxic effects were not different from those observed in intravenous infusion. Our results with intraarterial infusion of CDDP clearly indicate that this approach can deliver a high therapeutic efficacy in patients with advanced ovarian cancer with markedly less toxic effects.

Adolescent↗
PubMed1985

Improvement of dissolution and suppository release characteristics of flurbiprofen by inclusion complexation with heptakis(2,6-di-O-methyl)-beta-cyclodextrin.

The inclusion behavior of methylated beta-cyclodextrins, heptakis(2,6-di-O-methyl)-beta-cyclodextrin and heptakis-(2,3,6-tri-O-methyl)-beta-cyclodextrin in solution and the solid state was compared with that of natural beta-cyclodextrin using an anti-inflammatory drug, flurbiprofen, as a guest molecule. Stability constants were determined by the solubility method at various temperatures, and the thermodynamic parameters were calculated for inclusion complex formation in aqueous solution. The solid complexes were obtained in a molar ratio of 1:1, and their dissolution behavior and release from suppository bases were examined. The data suggest that the inclusion mode of the complex with 3 is somewhat different from that of the complexes with 1 and 2. From a practical point of view, 2 seems to be particularly useful for improving the pharmaceutical properties of flurbiprofen in various dosage forms.

Chemistry, Pharmaceutical↗
PubMed1985

Preparation of 13N-beta-phenethylamine.

Nitrogen-13-labelled beta-phenethylamine[( 13N]PEA) was synthesized by Hofmann rearrangement of [13N]phenylpropionamide prepared from phenylpropionyl chloride and aqueous [13N]ammonia solution. The reaction proceeded rapidly with a fairly good yield. [13N]PEA was isolated using preparative thin-layer chromatography, and organ distribution in mice was studied preliminarily. After i.v. administration of [13N]PEA, high accumulation and long-term retention of the radioactivity were observed in the brain and the heart.

Animals↗
PubMed1985

Globular Mallory-like bodies in renal cell carcinoma: report of a case and review of cytoplasmic eosinophilic globules.

The case of a patient who had renal cell carcinoma with cytoplasmic eosinophilic globular hyaline bodies antigenically similar to Mallory bodies, highly suggestive of globular type III alcoholic hyalin, is presented. Mallory bodies are not found exclusively in the liver, having also been demonstrated in the lung. The renal tumor in this case represents an additional extrahepatic site.

Aged↗