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Biomedical subjects

T Harada

Publications and source records attributed to T Harada.

At least 1,027 records · Page 57Linked to original sources
PubMed1984

[Evaluation of the pelvioureteral function through an impedance urine bolus-metry].

New equipment based on field gradient principle for measurement of cross sectional area of ureteral urine bolus has been developed. Inside the probe consisted of a Fr.4 ureteral catheter, there are 4 ring impedance electrodes and a bipolar ring ureteromyographic electrode. Ex vivo experiments has proved high values in reliability and reproducibility of obtained cross sectional area of urine bolus through an impedance method. Following results have been obtained through animal and clinical experiments. As urine bolus passed through the impedance electrodes, impedance represented the cross sectional area of urine bolus. The ureteral activity corresponded to the increase of urine flow changed as follows, ureteral peristaltic frequency increased, cross sectional area of urine bolus increased, length of urine bolus increased, during maximum diuresis, peristaltic activity diminished and continuous flow presented through ureteral column. It will be useful method for experimental or clinical evaluation of ureteral function.

Adult↗
PubMed1984

Tardive dyskinesia and neurotransmitters: effects of sodium valproate, cyproheptadine, oxypertine, hydroxyzine pamoate and Ca-hopantenate on monoamine metabolites, cyclic nucleotides and gamma-aminobutyric acid in human cerebrospinal fluid.

Lumbar cerebrospinal fluid (CSF) homovanillic acid (HVA), 3-methoxy-4-hydroxyphenylglycol (MHPG), 5-hydroxyindoleacetic acid (5-HIAA), cyclic AMP (cAMP) and cyclic GMP (cGMP) were measured in chronic schizophrenics with tardive dyskinesia before and three weeks after the initial treatment with sodium valproate (VPA), cyproheptadine, oxypertine or hydroxyzine pamoate. HVA levels significantly decreased after the administration of VPA, cyproheptadine or oxypertine. Cyclic GMP levels significantly increased after the administration of VPA or cyproheptadine. Elevation of the cAMP level was observed after the administration of VPA, cyproheptadine or oxypertine. An elevation of the MHPG level was observed during oxypertine treatment and a reduction of the 5-HIAA level was observed during hydroxyzine pamoate treatment. Decreases in HVA and increases in cGMP levels during treatment might be indicative of normalization of the dopaminergic-cholinergic imbalance in the brain. Lumbar CSF HVA and gamma-aminobutyric acid (GABA) were also measured in patients with tardive dyskinesia before and eight weeks after Ca-hopantenate treatment. No significant changes were observed before or after this treatment. The hypothesis is discussed that the pathogenesis of tardive dyskinesia may involve functional disorders not only of the dopaminergic or cholinergic system but also of the norepinephrinergic, serotoninergic and GABA-ergic systems.

Adult↗
PubMed1984

Proteinuria in amyloidosis correlates with epithelial detachment and distortion of amyloid fibrils.

To obtain a better understanding of the mechanism of proteinuria in amyloidosis we investigated the ultrastructural distribution and pattern of arrangement of glomerular amyloid fibrils in 15 patients. Clinical data were correlated with the ultrastructural features and with the morphometric indices obtained from composite electron micrographs of whole glomeruli. The epithelial side of amyloid deposits showed spicules (discrete pointed bundles of fibrils) and apparent fraying which we termed tufts (ill-defined and diffuse expansion of fibrils). The extent of mesangial amyloid deposition and total GBM deposition did not correlate with proteinuria. Current data suggest the importance of partial detachment of epithelial cells in pathogenesis of proteinuria, which in turn correlates with distortion of amyloid fibrils, spicules and tufts.

Adult↗
PubMed1984

Mucin-producing carcinoma of the thyroid gland--a case report and review of the literature.

Mucin-producing carcinoma of the thyroid is rare and its histogenesis has been debated. The first case was reported by Diaz-Perez et al. (1976). We herein report the sixth case of mucin-producing carcinoma of the thyroid which was discovered in a 72-year-old Japanese woman. This patient was admitted with a 10-year history of a lump in the right anterior neck, which started to grow suddenly two months before diagnosis. The pathological diagnosis at biopsy was anaplastic carcinoma. A course of radiation therapy followed by chemotherapy resulted in no response. She died of two months after admission. At autopsy, the main tumor was found in the right lobe of the thyroid with metastasis to the lungs and liver. Histologically, the tumor was composed of mucin-secreting glandular cells and nests of epidermoid cells with keratinization and infrequent formation of intercellular bridges. Mucin was demonstrated both intracellulary and extracellulary.

Adult↗
PubMed1983

Morphologic studies of the liver cell dysplasia.

Light and electron microscopic changes in human liver cells which were considered to be precancerous lesions, were studied. In our micrometrical examination, dysplastic liver cells were classified into two types: large and small dysplastic cell. Each type had nuclear pleomorphism and multinucleation; however, the nucleocytoplasmic ratio of the large dysplastic cell remained normal. Electron microscopically, the large dysplastic cell had some features of regenerative cells. The nucleocytoplasmic ratio of the small dysplastic cell was between that of normal hepatocytes and liver cancer cells. The difference in the incidence of the small and large dysplastic cells in normal livers and cirrhotic livers having hepatocellular carcinoma was statistically significant. In addition, the small dysplastic cell had more of a tendency to produce a small round focus. It was morphologically suggested that the more important candidate for the precancerous cell in the liver was the small dysplastic cell.

Aged↗
PubMed1983

Rate assay for determination of serum pseudo-cholinesterase activity.

A simple and reproducible method for the determination of serum pseudo-cholinesterase activity was developed by making use of a stable substrate, p-hydroxybenzoylcholine, with p-hydroxybenzoate hydroxylase as a linked enzyme. The method is based on spectrophotometric measurement of the decrease of NADPH. p-Hydroxybenzoate released from p-hydroxybenzoylcholine is hydroxylated by the action of p-hydroxybenzoate hydroxylase in the presence of NADPH and O2 to produce 3,4-dihydroxybenzoate and NADP+. This method is superior to the conventional methods in that this substrate is extremely stable up to pH 9.0, which is close to the optimum pH for the assay (pH 8.0). Serum interference was resolved by the use of p-hydroxybenzoate hydroxylase as a linked enzyme. The Km value of pseudocholinesterase for p-hydroxybenzoylcholine is 1 X 10(-5) M. The results of our method and Garry's method (Clin. Chem. 11, 91-96, 1965) correlated well (r = 0.962). The within-run and between-run C.V. values were 2.1 and 2.7, respectively.

4-Hydroxybenzoate-3-Monooxygenase↗