Search PubMed⌕ Search

Biomedical subjects

T Harada

Publications and source records attributed to T Harada.

At least 991 records · Page 55Linked to original sources
PubMed1985

Metastatic medullary thyroid cancer: localization with iodine-131 metaiodobenzylguanidine.

A patient in whom metastatic medullary thyroid cancer was diagnosed underwent a scintigraphic examination using [131I]MIBG. Multiple hot lesions and diffuse hepatic uptake were noted corresponding to bone and liver metastases. Iodine-131 MIBG may prove to be useful for scintigraphic localization and for the treatment of medullary thyroid cancer as in pheochromocytoma and neuroblastoma.

3-Iodobenzylguanidine↗
PubMed1985

Effects of aldose reductase inhibitor on retinal microangiopathy in streptozotocin-diabetic rats.

Wistar strain rats were made diabetic by injection of streptozotocin and divided into three groups fed on different diets: conventional solid foods, a fructose-rich diet and a fructose-rich diet mixed with ONO 2235, an aldose reductase inhibitor. The retinas of these rats were examined in flat mount preparations after trypsinization. Microvascular changes such as capillary tortuosity, microaneurysms, pericyte loss, that are typical of diabetic retinal microangiopathy, were seen most frequently in the rats fed on the fructose-rich diet. The rats fed on the fructose-rich diet with ONO 2235 showed much less vascular change than the diabetic rats fed on the conventional food. Electron microscopy of the retina revealed localized thickening of the basement membrane of the retinal capillaries, and this was most frequent in the fructose-fed rats. However, in rats fed on fructose with ONO 2235 the changes of the basement membrane were slight. It was concluded that the aldose reductase inhibitor, ONO 2235, prevented development of diabetic microangiopathy, probably by suppressing the enzymatic activation of aldose reductase in the retina.

Aldehyde Reductase↗
PubMed1985

[A case of renal cell carcinoma found in traumatic ruptured kidney].

A case of traumatic kidney rupture with pre-existing renal cell carcinoma in a 38-year-old male is reported. Macrohematuria appeared 48 hours after left precordial trauma in a traffic accident. Initial diagnosis at the emergency clinic was 9th rib fracture of his left chest alone and no other injury was recorded. A large mass in left abdomen was palpable at urologic OPD, however, and X-ray examination revealed a non-visualizing left kidney in IVP. After the ECHO, CT and arteriography findings gave an impression of left kidney rupture. Left nephrectomy accompanied by partial colectomy of descending colon by transperitoneal approach was performed 25 days after initial trauma. This delay was mainly due to disagreement of patient for surgical treatment because there were no subjective symptom except microhematuria and subsiding fever after transfer to the urologic section. En bloc removed specimen showed ruptured kidney with adhesiveness laterally to descending colon and harder core portion was found in midst of renal mass. Coronal slice of the specimen exhibited a renal parenchymal tumor with necrotic spots. Pathologic findings were complete kidney rupture in upper pole and clear cell type renal cell tumor was found in mid and lower pole. Also lymph nodes which obtained from left renal pedicle and para aortic margin proved no metastasis of tumor cell. Post operative clinical course was favourable without any recurrence for 15 months after surgery. Clinical incidence of type I and type II kidney trauma with pre-existing neoplastic change is considerably high, but the kidney ruptured case bearing renal cell carcinoma as a core shape is very rare.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1985

Antitumor activity of D-mannosamine in vitro: cytotoxic effect produced by mannosamine in combination with free fatty acids on human leukemia T-cell lines.

Cytotoxic effects of mannosamine and free fatty acids on human malignant T-lymphoid cell lines derived from patients with T-cell leukemia were investigated. The combination of mannosamine and an unsaturated fatty acid (oleate or linoleate) produced more striking cytotoxic effects on malignant lymphoid cells than on normal human lymphocytes. The amino sugars glucosamine or mannosamine in the combination caused a synergistic cytotoxic effect, while the other carbohydrates (N-acetylmannosamine, N-acetylglucosamine, or mannose) had little effect. On the other hand, the effect of saturated fatty acids (palmitate or stearate) in the same system was nil. An unsaturated fatty acid (oleate) caused an increase in lipid fluidity of the surface membrane in MOLT-4 lymphoid cells, which possess higher lipid fluidity in combination with mannosamine, while saturated fatty acids had no effect on the fluidity properties of the membrane lipids (even in the presence of mannosamine). The relationship between mannosamine and unsaturated fatty acids in cytolysis was discussed.

Carbohydrates↗
PubMed1985

Enzyme immunoassay of free thyroxin in dried blood samples on filter paper.

We describe a double-antibody enzyme immunoassay for determination of free thyroxin (FT4) in dried blood samples on filter paper, with use of a T4-beta-D-galactosidase complex. The measurable range of FT4 concentration in two 3-mm blood discs, each of which contained about 2.7 microL of blood, was 1.9 to 93 ng/L, as determined by comparison with concentrations of FT4 in known serum standards. FT4 in blood samples dried on filter paper was stable for at least four weeks when kept dry at -20 degrees C, room temperature, or 37 degrees C. The mean coefficients of variation were 7.6% (within assay) and 6.4% (between assays). Results for FT4 by this method correlated well with those for serum determined by radioimmunoassay (r = 0.98). The proposed method can be used to differentiate persons with hyper- and hypothyroidism from normal subjects and those with abnormal concentrations of thyroxin-binding globulin. The procedure seems suited for screening studies.

Adult↗
PubMed1985

Prurigo pigmentosa. Case report with an electron microscopic observation.

Prurigo pigmentosa is an inflammatory dermatosis characterized by pruritic, reddish, papular lesions and gross reticular pigmentation that occurs mainly on the trunk. Nearly 100 cases have been reported in Japan to date. We describe a patient with this condition who responded well to dapsone. An electron microscopic study of the reddish papular lesion showed marked intercellular edema and evidence of cellular injury of the basal cells. Direct immunofluorescence was negative. We stress that this condition is a distinct clinical entity, histologically characterized by a lichenoid tissue reaction as proposed by Pinkus, although it is little known outside Japan.

Adolescent↗
PubMed1985

[Posttraumatic aneurysm-like shadow at the supraclinoid portion of the internal carotid artery--a case report].

Traumatic cerebral aneurysms occupy less than 1% in all cerebral aneurysms and the onset usually occurs two to three weeks after the head injury. The authors present a case revealing an aneurysm-like shadow at the supraclinoid portion of the internal carotid artery immediately after the head injury. A 45-year-old man was admitted in a semi-comatose state, with bleeding from the nose and the ear. He presented anisocoria, with the left pupil larger, and absence of light reflex of the pupil. Roentgenographic examinations revealed fractures of the skull, the left radius, and the pelvis. CT disclosed subarachnoid hemorrhage and intrasylvian and intracerebral hematoma, with midline shift to the right. Left carotid angiograms revealed an aneurysm-like shadow projecting medially at the supraclinoid portion of the left internal carotid artery, and an emergency operation was performed. A left fronto-temporo-parietal craniectomy was done. Incision of the dura disclosed a thin subdural hematoma and subarachnoid hemorrhage on the surface of the brain. Following removal of the intrasylvian and intracerebral hematoma and slight retraction of the left frontal lobe, a bone fracture and dural tear of the anterior cranial fossa were found. During exposure of the internal carotid artery, a heavy bleeding occurred from the supraclinoid internal carotid artery. Under control of the bleeding by temporary clipping of the internal carotid artery, the bleeding point was proved to be a longitudinal tear of the infero-medial wall of the internal carotid artery, and its length was about 5 mm. Because it was difficult to suture the tear, the internal carotid artery was doubly clipped.(ABSTRACT TRUNCATED AT 250 WORDS)

Aneurysm↗
PubMed1985

A cause of discrepancy between values for urinary protein as assayed by the Coomassie Brilliant Blue G-250 method and the sulfosalicylic acid method.

In simultaneous assays of urinary proteins by the Coomassie Brilliant Blue G-250 (CBB) and the sulfosalicylic acid (SSA) methods, we noticed that about 18% of samples showed about twice higher protein values by the former method than by the latter. Some urinary proteins are soluble in SSA and react with CBB. Examinations with sodium dodecyl sulfate/polyacrylamide gel electrophoresis showed that these proteins migrated in 13 protein bands having relative molecular masses ranging from 15 000 to 230 000. The protein corresponding to the most intensely stained band in urine samples from the patients studied (with malignant tumors, renal disorders, etc.) had an Mr of 45 000; that in the pattern for healthy subjects had an Mr of 94 000. The former was identified as alpha 1-acid glycoprotein, the latter as Tamm-Horsfall mucoprotein.

Benzenesulfonates↗
PubMed1985

[Glycosylated hemoglobin and monitoring of diabetic retinopathy].

The value of glycosylated hemoglobin (HbA1) has been determined in diabetic patients with simple or proliferative retinopathy and in those without retinopathy. The mean value of HbA1 is significantly more highly elevated in patients with retinopathy than in those without retinopathy. Progressive augmentation of the value of HbA1 signifies a risk of deterioration of the retinopathy, whether it is a simple retinopathy or proliferative retinopathy.

Adult↗