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Biomedical subjects

T Hara

Publications and source records attributed to T Hara.

At least 901 records · Page 50Linked to original sources
PubMed1988

Specular microscopy of the anterior lens capsule after endocapsular lens implantation.

Endocapsular phacoemulsification and complete in-the-bag intraocular lens (IOL) fixation was performed on 77 eyes. Specular microscopy of the anterior lens capsule was performed postoperatively, and the process of anterior capsule opacification after endocapsular cataract surgery was classified. Lens epithelial cells on the inner surface of the anterior lens capsule, facing the optical portion of the IOL, underwent a diffuse fibrosis. In more peripheral areas, where the anterior and posterior capsules were tightly adherent, the regularly proliferating lens epithelial cells retained their polygonal shape. These findings suggest the possibility of retaining the transparency of the anterior lens capsule postoperatively by refilling the capsular bag to apply internal pressure.

Aged↗
PubMed1988

Effect of freezing on lens epithelial cell growth.

The effect of freezing on the growth of rat lens epithelial cells was studied in vitro. We found that 80% of the lens epithelial cells died after freezing at -45 degrees C for two hours and that the surviving cells could grow with the addition of growth factors or when placed on a sheet of type 4 collagen, but not when placed on a plain plastic culture dish. These results suggest that the surviving cells are at the Go phase of the cell cycle and that type 4 collagen or growth factors can initiate cell division.

Animals↗
PubMed1988

Light scattering caused by cells on the intraocular lens.

The anterior surface of a posterior chamber intraocular lens (IOL) was covered with cultured rat lens epithelial cells and light scattering was measured densitophotometrically. The light scattering intensity was maximum at a 30-degree scattering angle and was proportional to the number of cells per area on the IOL. Results suggests that the adhesion of lens epithelial cells on the IOL may influence its optical function.

Animals↗
PubMed1988

In vivo observation of the crystalline lens capsule.

The crystalline lens capsules of eye bank eyes, intraocular lens (IOL)-inserted eyes and those of monkey and rabbit eyes were observed in vivo and/or in vitro through a specular microscope or Scheimpflug slit images in combination with new image-processing techniques and scanning electron microscopy. Shagreen patterns seen in both human and animal eyes may reflect the lens epithelium and basement-membrane-like layer. As a postoperative examination in IOL-inserted eyes, the distance between the IOL and the posterior lens capsule could be measured with an accuracy of 50 microns.

Animals↗
PubMed1988

The structures of new antifungal antibiotics, benanomicins A and B.

Structures of new antifungal antibiotics, benanomicins A and B were determined to be N-[[5-[6-deoxy-3-O-(beta-D-xylopyranosyl)-beta-D-galactopyranosyloxy+ ++ ]-5, 6-dihydro-1,6,9,14-tetrahydroxy-11-methoxy-3-methyl-8,13-dioxobenzo++ + [a]naphthacen-2-yl]carbonyl]-D-alanine and N-[[5-[4-amino-4,6-dideoxy-3-O-(beta-D-xylopyranosyl)-beta-D- galactopyranosyloxy]-5,6-dihydro-1,6,9,14-tetrahydroxy-11-methoxy- 3-methyl-8,13-dioxobenzo[a]naphthacen-2-yl]carbonyl]-D-al ani ne, respectively, by spectral analyses and chemical degradation studies.

Acetylation↗
PubMed1988

[Effects of bifemelane on central dopaminergic and cholinergic systems in rats].

The study served to examine the effects of bifemelane on central dopaminergic-cholinergic neuronal mechanisms in rats. Bifemelane (5-20 mg/kg) evoked yawning responses, the frequency being low. Bifemelane (10 mg/kg) as well as bromocriptine (2.5 mg/kg) potentiated physostigmine (0.2 mg/kg)-, bromocriptine (2.5 mg/kg)- or apomorphine (0.1 and 0.5 mg/kg)-induced yawning but completely inhibited pilocarpine-induced yawning. Pretreatment with sulpiride (20 mg/kg) and a low dose of haloperidol (0.02 mg/kg) reversed the stimulatory effect of bifemelane on physostigmine-induced yawning and the inhibitory effect of the drug on pilocarpine-induced yawning, whereas atropine (5 mg/kg) diminished these yawning responses. SK&38393 (2.0 mg/kg), a dopamine D-1 receptor agonist, markedly potentiated bifemelane- and bromocriptine-induced yawning but inhibited physostigmine-induced yawning, and did not affect pilocarpine-induced yawning. The increased yawning responses were blocked by atropine and a low dose of haloperidol. Bifemelane (10 mg/kg) and bromocriptine (2.5 mg/kg) tended to increase apomorphine (5 mg/kg)-induced oral stereotypy, such as licking and biting, but the increase was not significant. These results suggest that the effects of bifemelane on central dopaminergic and cholinergic neurons may be similar to those of bromocriptine.

2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-ben↗
PubMed1988

A novel leukemia cell line, MR-87, with positive Philadelphia chromosome and negative breakpoint cluster region rearrangement coexpressing myeloid and early B-cell markers.

We developed a Philadelphia chromosome (Ph) positive cell line, designated MR-87, from a 4-year-old boy with Ph+-acute leukemia. MR-87 cells grew in single cell suspensions with a doubling time of 120 to 144 hours. Both MR-87 and original leukemia cells were positive for myeloperoxidase (MPO) and myeloid antigen CD13. These cells exhibited the early B-cell phenotype, ie, terminal deoxynucleotidyl transferase+, Ia+, CD19+, and CD10+. Rearrangement of the immunoglobulin heavy chain was confirmed in both. Approximately 80% of MR-87 cells coexpressed CD13 and lymphoid antigens CD10 or CD19, as confirmed by a two-color analysis. Simultaneous expression of MPO and CD19 on a single MR-87 cell was demonstrated at ultrastructural level. Thus, MR-87 is a Ph+ leukemia cell line exhibiting a hybrid phenotype. The breakpoint cluster region (bcr) was not rearranged in the MR-87 cells and subsequent analysis using antisera revealed that these cells expressed a novel protein, P190c-abl, which was immunoprecipitated with anti-abl and anti-phosphotyrosine antibodies. The MR-87 line will be most useful for investigating the biology and pathogenesis of Ph+ bcr- acute leukemia.

Antigens, Differentiation, B-Lymphocyte↗
PubMed1988

[Clinical study of cefuzonam in the field of obstetrics and gynecology].

Cefuzonam (CZON, L-105) was used clinically for the treatment of obstetrical and gynecological infections at a dosage of 1 g once or twice daily by intravenous drip infusion. The results obtained are summarized as follows. 1. Clinical effects of CZON were analyzed in 10 patients, including 5 patients with intrapelvic infections, 3 with intrauterine infections, and 1 each with adnexitis and an external genital infection. Excellent responses were observed in 1 patient (11.1%), good responses in 7 (77.8%), poor responses in 1 (11.1%), and one remaining case was unevaluable. The efficacy ratio was 88.9%. 2. Upon the treatment, eradications of causative bacteria were observed in all 4 cases tested. Staphylococcus sp. (2 strains), Staphylococcus epidermidis (1 strain), Klebsiella oxytoca (1 strain) and Pseudomonas putida (1 strain) were all eradicated by the CZON treatment. 3. The safety of the drug was analyzed in the 10 patients and rash occurred in 1 patient as a side effect. 4. One patient showed eosinophilia after the CZON treatment. It appeared that CZON would be useful for the treatment of obstetrical and gynecological infections.

Adult↗
PubMed1988

[Positron emission tomography using pyruvate-1-11C on mitochondrial encephalomyopathy].

In order to investigate in vivo metabolism of pyruvate in the brain, positron emission tomography (PET) using pyruvate-1-11C were performed on two patients with mitochondrial encephalomyopathy, one patient with Leigh disease and one patient with epilepsy. PET images of epilepsy showed less RI uptakes in brain tissues than in skull muscles. And RI was cleared rapidly from the brain tissues. But PET images of mitochondrial encephalomyopathies showed increased RI uptakes and slow RI clearance in cerebral cortices, basal ganglia and thalamus. Also RI was cleared slowly or accumulated in ventricles. This suggested that pyruvate metabolism may be impaired with metabolical trapping of 11C by lactic acids, since it is said that 11C of pyruvate-1-11C is cleared rapidly from the normal brain tissues by decarboxylation of pyruvate. The usefulness of PET studies with pyruvate-1-11C are suggested for investigating pyruvate metabolism in brain tissues, and for diagnosing mitochondrial encephalomyopathy.

Brain↗