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Biomedical subjects

T Garam

Publications and source records attributed to T Garam.

27 records · Page 2Linked to original sources
PubMed1981

[Association between natural cell mediated cytotoxicity (NCMC) and serum factors in acute leukemia and other malignant diseases].

Natural cell-mediated cytotoxicity (NCMC) of human peripheral lymphocytes against allogeneic tumor line (K-562) was substantially increased by the AB serum. Dialysis, a well as Sephadex G-200 gel filtration of AB serum revealed that the immunoglobulin containing fraction was the responsible factor for the increase. Results suggest the involvement of antibodies in NCMC. Autologous and allogeneic serum of patients with gynecological carcinoma inhibited (10-64%) and/or stimulated (10-250%) the NCMC activity in the patients. Residual NCMC activity exceeding 20% has been present after the trypsin digestion in serum free medium of effector lymphocytes. A similar 20% residual activity has been observed after the addition of anti-immunglobulin and antigen-antibody complexes, respectively. Our findings suggested the role of antibodies and serum factors in natural killer activity. Nonetheless other mechanisms resistant to trypsin treatment and of a certain specificity to tumor cells may not be excluded.

Animals↗
PubMed1981

[Angioimmunoblastic lymphadenopathy].

Cytological, histological and ultrastructural characteristics of a cose of an extranodal and nodal angioimmunoblastic lymphadenopathy persisting during several years are given. Angiomatous proliferation in the case reported seemed to be identical both in the lymph node and in the cutaneous infiltration. Types of cells seen in the angioimmunablastic lymphadenopathy did not differ from those of other immunoreactive processes. In the extranodal infiltrates in addition to other phenomena thickening of the immunological techniques decrease of the natural cytotoxicity and the number of T-lymphocytes could be observed.

Autoantibodies↗
PubMed1981

Comparison of the natural cell mediated and antibody dependent cell mediated cytotoxicity measured as a function of target cell number in patients with mammary tumors and healthy female blood donors.

Peripheral blood lymphocytes of 84 patients with mammary tumors (73 malignant, 11 benign diseases) and 79 healthy female blood donors were tested in three cytotoxicity assays. Natural cell mediated cytotoxicity (NCMC) was measured against K562 cell line, antibody dependent cellular cytotoxicity (ADCC) was tested against K562 cell line sensitized with hyperimmune rabbit anti K562 sera and human 0, Rh positive (C, D, E pos.) erythrocytes sensitized with Ripley antibodies. Effector cell activity was measured as a function of the target cell number in the new cytotoxic capacity test. Maximums were determined experimentally and also by extrapolation from the measured values using the Michaelis-Menten equation. It was found, that activity of patients was lower than that of the controls in all the three tests already at an early stage of the malignant disease. Therefore introduction of the new cytotoxic capacity test to screen NCMC and ADCC is suggested.

Antibody-Dependent Cell Cytotoxicity↗
PubMed1979

Correlation between effector lymphocytes in natural and antibody-mediated cytotoxicity.

Human sera enhanced spontaneous cell-mediated cytotoxicity (SCMC), while anti-IgG (Fab') 2 treatment decreased this cytotoxic activity of human lymphocytes for an in vitro growing cell line (K--562). Trypsin treatment of the effector cells considerably decreased the cytotoxic potential. However, a significant cytotoxic activity could always be found in serum-free medium. While these findings suggest the involvement of antibodies in the SCMC, they also reflect the existence of serum-indpendent (sui generis) SCMC activity of lymphocytes. Removal of SCMC of Fc receptor bearing effector cells was performed by target cell adherence (rosetting). Separation of the target cell-bound lymphocytes was done by centrifugation on special Ficoll gradient. The depletion of SCMC effector cells resulted in a 62% reduction of SCMC and in a 39% reduction of ADCC. On the other hand, removal of Fc bearing effector cells showed a similar reduction in both ADCC (66%) and SCMC (78%). Our results suggest that SCMC represents a complex activity, arising partly from the interactions of certain serum-derived or lymphocytes surface-bound antibodies and partly from a spontaneous cytotoxic function of the effector cells. It is possible that the effector cells involved in both SCMC and ADCC derive from the same lymphocyte population and the differences are due mainly to the lower number of SCMC effector cells.

Animals↗