Search PubMed⌕ Search

Biomedical subjects

T Furuya

Publications and source records attributed to T Furuya.

At least 163 records · Page 9Linked to original sources
PubMed1993

Biotransformation of (-)-borneol by cultured cells of Eucalyptus perriniana.

Six new biotransformation products, (-)-borneol 2-O-beta-gentiobioside, (-)-borneol 2-O-beta-sophoroside, (-)-borneol 2',6'-di-O-(beta-D-glucopyranosyl)-beta-D-glucopyranoside, 6-exo-hydroxyborneol 2-O-beta-D-glucopyranoside, 4-hydroxyborneol 2-O-beta-D-glucopyranoside and 5-exo-hydroxyborneol 2-O-beta-gentiobioside, together with 5-exo-hydroxyborneol 2-O-beta-D-glucopyranoside, were isolated from jar fermentor cultures of Eucalyptus perriniana following administration of (-)-borneol.

Biotransformation↗
PubMed1993

Biotransformation of 18 beta-glycyrrhetinic acid by ginseng hairy root culture.

On administration of 18 beta-glycyrrhetinic acid to hairy root cultures of Panax ginseng, three new biotransformation products, 30-O-[beta-D-glucopyranosyl-(1-->2)-beta-D- glucopyranosyl]18 beta-glycyrrhetinic acid, 30-O-(6- O-malonyl-beta-D-glucopyranosyl)18 beta-glycyrrhetinic acid and 3-O-[6-O- malonyl-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranosyl]18 beta-glycyrrhetinic acid, were isolated together with three known compounds. Ginseng hairy roots showed the biotransformation abilities of glycosylation and malonylation to 18 beta-glycyrrhetinic acid.

Biotransformation↗
PubMed1993

Use of body fluid of adult female Ascaris suum as an antigen in the enzyme-linked immunosorbent assay (ELISA) for diagnosis of swine ascariosis.

The Enzyme-linked immunosorbent assay (ELISA) was used to detect Ascaris suum antibodies in swine sera with adult body fluid (ABF) as an antigen. The assay was standardized with respect to the antigen concentration and serum and conjugate dilutions. Cross reaction was found between the antigen and the sera from the swine infected with Metastrongylus apri. The ELISA was more sensitive than the complement fixation test. Five protein peaks were obtained from ABF by gel filtration on Sephacryle S-300. Fraction 1 was the most specific. A 105 kDa protein in the fraction reacted with swine IgG in the serum of infected animals in Western blot analysis.

Animals↗
PubMed1993

Inhibitory effects of berberine-type alkaloids on elastase.

Berberine-type alkaloids and some structurally related alkaloids were tested for inhibitory activity on porcine pancreatic elastase (PPE) and human sputum elastase (HSE). The chlorides of berberine, coptisine, and sanguinarine significantly inhibited the elastolytic activity of both the enzymes, but tetrahydroberberine had no effect on the activity. It seems that the quaternary nitrogen of the alkaloids plays an important role in the inhibition of elastolytic activity. Amidolytic activity of the elastases was not affected by any of the alkaloids tested.

Amino Acid Sequence↗
PubMed1993

Graft-versus-host disease after brown Norway-to-Lewis and Lewis-to-Brown Norway rat intestinal transplantation under FK506.

In LEW rats treated daily with variable doses of FK506 for 14 days and weekly thereafter, successful intestinal transplantation from fully allogeneic BN donors never was complicated by fatal GVHD. In contrast, with LEW-to-BN transplantation, rejection was difficult to control and GVHD developed after the end of the daily treatment. However, FK506 in high daily doses continued after the initial 14-day course could prevent this GVHD or even reverse it after allowing its onset. Further experiments did not clarify why the BN rat was an "easy" donor and "difficult" recipient. In unaltered animals the lymphocyte population of normal LEW rats had a higher proportion of T cells, fewer B cells, and a lower CD4:CD8 ratio than normal BN rats. However, one-way MLR reactions of the BN and LEW combinations were generally similar in either direction and not affected differently by the addition of FK506 to the medium. The two-way lymphocyte traffic from graft to host lymphoid organs and vice versa also was similar with BN-to-LEW and LEW-to-BN models. The BN rat may be a useful tool to investigate inadequately explained mechanisms of GVHD.

Animals↗
PubMed1993

Three different cellular proteins bind to complementary sites on the 5'-end-positive and 3'-end-negative strands of mouse hepatitis virus RNA.

The termini of viral genomic RNA and its complementary strand are important in the initiation of viral RNA replication, which probably involves both viral and cellular proteins. To detect the possible cellular proteins involved in the replication of mouse hepatitis virus RNA, we performed RNA-protein binding studies with RNAs representing both the 5' and 3' ends of the viral genomic RNA and the 3' end of the negative-strand complementary RNA. Gel-retardation assays showed that both the 5'-end-positive- and 3'-end-negative-strand RNA formed an RNA-protein complex with cellular proteins from the uninfected cells. UV cross-linking experiments further identified a 55-kDa protein bound to the 5' end of the positive-strand viral genomic RNA and two proteins 35 and 38 kDa in size bound to the 3' end of the negative-strand cRNA. The results of the competition assay confirmed the specificity of this RNA-protein binding. No proteins were found to bind to the 3' end of the viral genomic RNA under the same conditions. The binding site of the 55-kDa protein was mapped within the 56-nucleotide region from nucleotides 56 to 112 from the 5' end of the positive-strand RNA, and the 35- and 38-kDa proteins bound to the complementary region on the negative-strand RNA. The 38-kDa protein was detected only in DBT cells but was not detected in HeLa or COS cells, while the 35-kDa protein was found in all three cell types. The juxtaposition of the different cellular proteins on the complementary sites near the ends of the positive- and negative-strand RNAs suggests that these proteins may interact with each other and play a role in mouse hepatitis virus RNA replication.

Animals↗
PubMed1993

An outbreak of Salmonella typhimurium infection in bengalees, a variety of Lonchura striata.

Bengalees (a variety of Lonchura striata) in an aviary in Nagano Prefecture died of severe diarrhea in 1991. Pathological examination revealed slight enlargement of the liver and spleen. Histopathologically, necrotic granulomatous lesions were seen in the liver. Salmonella Typhimurium was isolated from the main organs and fecal samples. No evidence of Newcastle disease or chlamydiosis was detected. The disease was finally diagnosed as S. Typhimurium infection in bengalees. In spite of some countermeasures, 198 out of 268 birds died within 6 months, a mortality rate of 74%. Epizootiological survey revealed that the infection was transmitted by birds introduced from another prefecture. This is the first report of S. Typhimurium infection in bengalees.

Animals↗
PubMed1993

Reverse interference method for measurement of hog cholera virus (HCV) and anti-HCV antibody.

A new procedure was developed for the assay of the hog cholera virus (HCV) and anti-HCV antibody. Initially, the suppression effect of HCV on interferon (IFN) by HCV production was confirmed. Swine kidney cell cultures preinfected with HCV produced no IFN, even following the addition of IFN inducers. However the sensitivity of the cell to IFN was not influenced by the infection with this virus. Based on these results, a new method, named reverse interference method, was established. In this method, infective titer of HCV was determined by the appearance of cell pathogenic effects (CPE) induced by vesicular stomatitis virus (VSV), which is caused by the suppression effect on the heterologous interference of GPE- strain of HCV against VSV infection in swine kidney cell cultures. This method showed nearly the same sensitivity as the END method. There was no difference in the infective titer of HCV and antibody titer against HCV as estimated by this method and the END method. The reverse interference method had advantages in rapidity and objectivity compared with the END method.

Animals↗
PubMed1993

Attention state in electrodermal activity during auditory stimulation of children with attention-deficit hyperactivity disorder.

Electrodermal activity during passive and active listening tasks of 18 children with Attention-Deficit Hyperactivity Disorder (ADHD) and 49 healthy school children was studied. The procedure included baseline recording, a passive listening task, instructions, and both simple and discriminative active-listening tasks. ADHD subjects tended to exhibit lower arousal as indicated by the decrease in amplitude of the skin conductance response. Present findings confirm classical observation that ADHD children have shorter attention spans.

Acoustic Stimulation↗
PubMed1993

[The local relapse of prostatic cancer and initial local treatment].

We treated fifty cases of prostatic cancer that had no distant metastasis and no other primary cancers between October 1983 and September 1990. Our initial treatment for clinical stages A2, B1, B2 included radical prostatectomy, pelvic lymphadenectomy, and bilateral orchiectomy. Staging lymphadenectomy with bilateral orchiectomy was applied to patients with clinical stage C tumor, and if there was no lymph node metastasis, external beam radiation therapy for the prostate was performed. Patients who did not consent to major surgeries or had poor general status or had clinical stage D1 tumor underwent bilateral orchiectomy. Mean observation period was forty-one months. Eleven cases relapsed. Of the eleven cases, ten had local relapse at the diagnosis of relapse (two of them had distant metastasis simultaneously, six of them had distant metastasis later, and two of them had no distant metastasis in the observation period). All these ten cases had no underwent the initial local treatment. The remainder who had underwent radical prostatectomy relapsed with distant metastasis alone. This study suggests that the local relapse tends to precede distant metastasis as the mate of relapse of prostatic cancer excluding stage D2. There is a possibility that initial local treatment is useful improving the prognosis of the patients for clinical stage A2-D1 prostatic cancer.

Adenocarcinoma↗
PubMed1993

[Evaluation of immunotoxicity tests using cyclosporin A-treated rats: the International Collaborative Immunotoxicity Study II (cyclosporin A)].

Immunotoxicological effects of cyclosporin A (CsA) were studied by enhanced histopathological and functional tests in rats. Male F344 rats were orally administered with CsA in doses of 0, 2.5, 10, and 40 mg/kg/day for 28 successive days. Hematological examination revealed that the CsA treatment brought about a marked dose-dependent decrease in the number of WBCs, which was attributed to a decrease in the number of lymphocytes. In the femoral bone marrow, a significant reduction in the number of nucleated cells was observed, which was attributed to a decrease in the number of lymphocytes and erythroblasts. Histopathologically, diminution of thymic medullas, appearance of tangible body macrophages in thymic cortices, and calcification and basophilic changes in kidneys were observed in the middle and high dose groups. Immunohistological examination with anti-rat T lymphocyte antibody showed a decrease in the number of T cells at the periarterial lymphatic sheaths in the spleens. As for the functional tests, CsA treatment remarkably reduced the PFC number even in the low dose group. The Con A response of spleen cells was decreased in the middle and high dose groups. The STM response was reduced only in the high dose group. The NK activity was little affected. Thus, in the CsA-treated F344 rats, the enhanced histopathological and some functional tests which were proposed by ICICIS, were found to be useful to detect damages to the immune system.

Administration, Oral↗
PubMed1993

[Complete resection of left apical invading lung cancer, following reconstruction of subclavian artery by artificial graft--a case report].

A cumurative operation for apical invading lung cancer was performed in a 49-year-old man. Left upper lobectomy was combined with resection of the chest wall, left subclavian artery and brachial nerve, following reconstruction of the left subclavian artery with an artificial vessel graft. Our approach involved sternotomy, antero-lateral thoracotomy and cutting of the clavicle. This approach is useful for apical lung cancer without wide invasion of the posterior chest wall, as in this patient. Although radiotherapy and chemotherapy were administered, the patient succumbed to hematogenous metastases 15 months after the operation. We suggest that adjuvant therapy may be needed for apical invading lung cancer, even if the tumor is believed to be completely resected.

Adenocarcinoma↗
PubMed1993

[Usefulness of (1-->3)-beta-D-glucan measurement for diagnosis of deep mycosis].

The number of deep mycosis has been increasing because of increases in immunocompromised hosts and in fungal colonization associated with increasing use of broad-spectrum antibacterial antibiotics. Based on these phenomenon, a simple test method for an early diagnosis of deep mycosis is urgently desired. We therefore investigated the usefulness of assaying a fungal cell component, (1-->3)-beta-D-glucan (beta-glucan). The amount of beta-glucan was obtained from the difference between the amounts determined using Toxicolor and Endospecy, and the serum levels of more than 10 pg/ml were considered positive signs for beta-glucan. The following results were obtained: We found that beta-glucan was positive in 75% of the patients who had been definitely diagnosed to have mycosis, and in 58.3% of the patients strongly suspected of mycosis. The numbers of beta-glucan positive patients' in these 2 groups of patients were significantly different from that in those without mycosis (14.7%, P < 0.05). Thus a usefulness of beta-glucan measurement for the diagnosis of mycosis was demonstrated. However, beta-glucan was sometimes negative even in patients with fungemia at an early phase of the disease and turned positive several days later. Even in a patient with definite lung mycosis, who had a latent circumscribed lesion (afebrile and CRP-negative), beta-glucan was also negative. From these findings, one should be aware that the beta-glucan test produces false negatives even in patients with definite mycosis and that the test should be repeated during the course of the disease.

Aged↗
PubMed1993

Liver allograft rejection in sensitized recipients. Observations in a clinically relevant small animal model.

A sequential analysis of liver allograft rejection in sensitized rats using immunopathological and ultrastructural microscopy is described. Lewis rats were primed with four ACI skin grafts and challenged with an arterialized ACI orthotopic liver allograft 14 to 17 weeks later. The sensitization resulted in a mix of IgG and IgM lymphocytotoxic antibodies at a titer of 1:512 at the time of transplantation. Specificity analysis of pretransplant immune sera revealed a predominance of IgG anti-class I major histocompatibility complex (RT1) antibodies with a minor IgG fraction showing apparent endothelial cell specificity (non-RT1). This level of sensitization was associated with accelerated graft failure in 3 to 5 days from mixed humoral and cellular rejection. Sequential analysis of serial posttransplant graft biopsies revealed diffuse vascular IgG deposition and platelet thrombi in portal veins and periportal sinusoids within 3 minutes after reperfusion. This was followed by endothelial cell hypertrophy and vacuolization, periportal hepatocyte necrosis, arterial spasm, focal large bile duct necrosis, and hilar mast cell infiltration and degranulation. However, the liver allografts did not fail precipitously and hyperacute rejection was not seen. Kupffer cell phagocytosis of the sinusoidal platelets began as early as 30 minutes posttransplant and by 24 hours, the platelet thrombi had decreased. Cholangioles appeared focally at the edge of the limiting plates by 2 to 3 days, apparently in response to earlier periportal hepatocyte damage. A mononuclear portal and perivenular infiltrate became evident at 3 days, and graft failure was attributed to both antibody and cell-mediated rejection (Furuya et al: Preformed lymphocytotoxic antibodies: Hepatology 1992, 16: 1415-1422). The model described resembles observations in crossmatch positive human liver allograft recipients. The mechanisms of hepatic graft resistance to antibody mediated rejection and the possible long term consequences of early damage to the biliary tree are discussed.

Animals↗