Search PubMed⌕ Search

Biomedical subjects

T Furuya

Publications and source records attributed to T Furuya.

At least 145 records · Page 8Linked to original sources
PubMed1995

[Primary biliary cirrhosis in patients with Sjögren's syndrome].

Primary biliary cirrhosis (PBC) is one of the most frequent hepatic diseases associated with Sjögren's syndrome (SS), its reported incidence ranging from 3% to 9%. There is much association between SS and PBC in the pathogenesis. In both diseases, autoimmunity seems to be directed toward the ductal epithelial cells. Epstein Barr Virus may contribute to PBC, as well as SS. PBC associated with SS tends to be asymptomatic and to be in an early stage in histologically. Antimitochondrial antibody (AMA) is the most sensitive indicator of PBC in primary SS. When SS patients have an elevated alkaline phosphatase level, it is necessary to examine AMA and to perform liver biopsy.

Adult↗
PubMed1994

Structures of the human and mouse growth inhibitory factor-encoding genes.

Growth inhibitory factor (GIF) is down-regulated in Alzheimer's disease (AD) brains. To analyze the mechanism of this down-regulation, we isolated the human and mouse GIF genes. These genes consist of three exons, are approx. 1-kb long and show strikingly high homology to metallothionein-encoding genes. A comparison of the human and mouse GIF showed several conserved sequences, including the putative AP-2, SP-1, TATA-binding protein and metal-responsive elements (MRE). A sequence similar to the human gfa common sequence (hgcs), recently identified as the sequence for an astrocyte-specific transcriptional factor, is present in the promoter of these GIF. Characterization of factors associated with the putative regulatory elements in the promoter of GIF should help in determining the mechanism of the down-regulation of GIF in AD brains.

Alzheimer Disease↗
PubMed1994

6-(1H-imidazol-1-yl)-7-nitro-2,3(1H,4H)-quinoxalinedione hydrochloride (YM90K) and related compounds: structure-activity relationships for the AMPA-type non-NMDA receptor.

A novel series of quinoxalinediones possessing imidazolyl and related heteroaromatic substituents was synthesized and evaluated for their activity to inhibit [3H]AMPA binding from rat whole brain. From the structure-activity relationships, it was found that the 1H-imidazol-1-yl moiety could function as a bioisostere for the cyano and nitro groups, and that 6-(1H-imidazol-1-yl)-7-nitro-2,3(1H,4H)-quinoxalinedione (11) showed the most potent activity for the AMPA receptor. Compound 11 was evaluated for selectivity versus other excitatory amino acid receptors, and its action against AMPA at its receptor in the rat striatum was characterized. These data showed that compound 11 was a selective antagonist for the AMPA receptor with a Ki value of 0.084 microM, being approximately equipotent with 2,3-dihydro-6-nitro-7-sulfamoylbenzo(f)quinoxaline (3) (NBQX; Ki = 0.060 microM). Compound 11 was also found to give protection against sound-induced seizure on DBA/2 mice at the minimum effective dose of 3 mg/kg ip (3; 10 mg/kg ip).

Acoustic Stimulation↗
PubMed1994

Cycloartane triterpene glycosides from the hairy root cultures of Astragalus membranaceus.

Agroastragaloside II, a new astragaloside was isolated from the hairy root culture of Astragalus membranaceus. Its structure was established as 3-O-beta-(2'-O-acetyl)-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-(24S)- 3 beta,6 alpha,16 beta,24,25-pentahydroxy-9,19-cyclolanostane on the basis of spectroscopic data. Three known astragalosides, astragaloside II, isoastragaloside I and 3-O-beta-D-xylopyranosyl-cycloastragenol were also isolated.

Astragalus propinquus↗
PubMed1994

Acylated saponins, masonosides A-C, from the corms of Crocosmia masoniorum.

Three novel triterpenoid saponins, named masonosides A-C, were isolated from the corms of Crocosmia masoniorum. On the basis of spectral and chemical evidence, the structures of masonosides A-C were elucidated as 3-O-[alpha-L-arabinopyranosyl-(1-->6)-beta-D-glucopyranosyl]-28-O- (2-O- [beta-D-apiofuranosyl-(1-->4)-beta-D-xylopyranosyl-(1-->4)- alpha-L-rhamnopyranosyl]-3-O-(beta-D-glucopyranosyl)-4-O-(2-hydroxy-9-ox o-16- alpha-L-rhamnopyranosyloxyhexadecanoyl)- beta-D-fucopyranosyl)-polygalacic acid, 3-O-[alpha-L-arabinopyranosyl-(1-->6)- beta-D-glucopyranosyl]-28-O-(2-O-[beta-D-apiofuranosyl-(1-->4)-bet a-D- xylopranosyl-(1-->4)-alpha-L-rhamnopyranosyl]- 3-O-(beta-D-glucopyranosyl)-4-O-(2,16-dihydroxy- 9-oxohexadecanoyl)-beta-D-fucopyranosyl)-polygalacic acid and 3-O-[alpha-L-arabinopyranosyl-(1-->6)-beta-D-glucopyranosyl]- 28-O-(2-O-[beta-D-xylopyranosyl-(1-->4)-alpha-L-rhamnopyranosyl]-3-O- (beta-D-glucopyranosyl)-4-O-(2,16-dihydroxy- 9-oxohexadecanoyl)-beta-D-fucopyranosyl)-polygalacic acid, respectively.

Carbohydrate Sequence↗
PubMed1994

Biotransformation of (+)-camphor by cultured cells of Eucalyptus perriniana.

Seven new biotransformation products were isolated from a jar fermentor culture of Eucalyptus perriniana following administration of (+)-camphor. The main product was (1S,4R,6S)-6-hydroxybornan-2-one 6-O-beta-D-glucopyranoside. The minor components were (1S,4R,6R)-6-hydroxybornan-2-one 6-O-beta-D-glucopyranoside, (1R,4S,6S)-6-hydroxybornan-2-one 6-O-beta-D-glucopyranoside, (1R,4R,5R)-5-hydroxybornan-2-one 5-O-beta-D-glucopyranoside, (1R,3R,4S)-3-hydroxybornan-2-one 3-O-beta-D-glucopyranoside, (1R,4R,7R)-8-hydroxybornan-2-one 8-O-beta-D-glucopyranoside and 2-(4-oxo-2,2,3-trimethylcyclopentyl)-ethyl-beta-D-glucopyranoside. All products were mono-glucosides and the oxygen function was introduced before glucosylation. Reduction of the ketone group of camphor was not observed.

Biotransformation↗
PubMed1994

Minor desacylsaponins from the corms of Crocosmia masoniorum.

Two novel triterpene saponins, named desacylmasonosides 4 and 5, and a new oxygenated fatty acid have been isolated, together with a known saponin, desacylcrocosmioside A, from the weak alkaline hydrolysate of the crude saponin obtained from the corms of Crocosmia masoniorum. On the basis of spectral and chemical evidence, the structures of desacylmasonosides 4 and 5 were elucidated as 3-O-[beta-D-fucopyranosyl-(1-->6)-beta-D-glucopyranosyl]-28-O-(2-O-[beta - D-apiofuranosyl-(1-->4)-beta-D-xylopyranosyl-(1-->4)-alpha-L- rhamnopyranosyl]-3-O-(beta-D-glucopyranosyl)-beta-D-fucopyranosyl)-po lygalaci c acid and 3-O-[alpha-L-arabinopyranosyl-(1-->6)-beta-D-glucopyranosyl]-28-O- [2-O-(alpha-L-rhamnopyranosyl)-3-O-(beta-D-glucopyranosyl)-beta-D- fucopyranosyl]-polygalacic acid, respectively. An oxygenated fatty acid derived from the acyl moiety of the esterified saponins was identified as 2,16-dihydroxy-9-oxohexadecanoic acid.

Carbohydrate Sequence↗
PubMed1994

Method for selecting anthocyanin-producing cells by a cell sorter.

We found a novel method to select anthocyanin-producing cells by a new means of cell staining. After staining with 0.2 ppm fluorescein isothiocyanate (FITC), the protoplasts from Aralia cordata cultured cells, which were composed of high anthocyanin-producing cells and non-producing cells, were observed to be distinguishable under a fluorescence microscope. The green fluorescence of FITC from the non-producing cells was clearly ascertained by the naked eye. On the other hand, the fluorescence of the anthocyanin-producing cells was completely missing. This phenomenon resulted from compensation of the green fluorescence (lambda max 525 nm) of FITC and the green light absorption (lambda max 530 nm) of anthocyanin. Although investigations of the flow cytometric method were developed on the basis of this phenomenon, the histograms of these protoplasts expressed the distinction between the anthocyanin-producing cells and the non-producing cells. In order to prove the technique, a highly productive cell line was selected from the heterogeneous populations of anthocyanin-producing or non-producing protoplasts from A. cordata cultured cells. The number of the sorted protoplasts containing anthocyanin was counted by the naked eye, the anthocyanin-producing protoplasts were approximately 90% in the whole protoplasts and the viability was 60-70%. Anthocyanin concentration the sorted protoplasts was 3-fold higher as compared with the protoplasts before sorting. The anthocyanin content of the cell line selected by this method was 7.7 +/- 0.6%/g dw.(ABSTRACT TRUNCATED AT 250 WORDS)

Anthocyanins↗
PubMed1994

Intracellular localization and DNA-binding activity of a class of viral early phosphoproteins in human fibroblasts infected with human cytomegalovirus (Towne strain).

Indirect immunofluorescence (IF) with monoclonal antibody M23 prepared against the nuclei of human embryo lung (HEL) cells infected with human cytomegalovirus (HCMV) Towne strain showed that the M23 antigen reactive with the M23 antibody was localized within distinct foci throughout the nucleus of infected HEL cells shortly after infection, even at 2 h post-infection (p.i.). The foci increased in size by 24 h p.i. and then the IF patterns changed to show the nuclear inclusion body-like structures at 72 h p.i. Treatment with phosphono-acetic acid, a HCMV DNA replication inhibitor, resulted in a nuclear pattern similar to that observed shortly after infection. The double-labelled IF test revealed that the HCMV UL44 antigen essential for viral DNA replication colocalized with the M23 antigen in the same intranuclear structure shortly after infection whereas neither viral antigen appeared to colocalize in most cells later after infection. The M23 antibody immunoprecipitated four proteins. 34K, 43K, 50K and 84K, in infected cells. To examine whether these proteins correspond to four early phosphoproteins encoded by the HCMV strain AD169 genome, the Towne strain DNA sequence corresponding to that encoding both the 34K and 43K proteins of strain AD169 was determined and transiently expressed in COS-7, Vero and HEL cells. These proteins were detected by the M23 antibody within the foci of these nuclei as found in the nuclei of productively infected cells shortly after infection. In addition, the 34K, 43K and 50K proteins at least were shown to be DNA-binding proteins by double- and single-stranded DNA-cellulose column chromatography. The relationship of these proteins to the status of viral DNA replication is discussed.

Amino Acid Sequence↗
PubMed1994

Use of running exercises for preschoolers with mental retardation as a means of improving play or school activity.

Various physical training programs have been devised to achieve greater physical fitness for handicapped children who otherwise exercise insufficiently. Such training may also facilitate socializing and play at school. Running exercises were conducted with 4 handicapped preschoolers and 3 age-matched normal preschoolers for 3 30-min. sessions per week for 11 weeks. Measures were seconds taken to run 25 meters and ratings of play behavior. Analysis showed that children who reluctantly joined the exercise made little progress in running. The quality of movement and play activities in nursery school and interactive behavior improved significantly for participants who also improved in running.

Attention↗
PubMed1994

Age differences in characteristics of the attention process of electrodermal activity during auditory stimulation.

Age differences in characteristics of the attention process of electrodermal activity during passive and active listening tasks were studied with 43 nursery and 49 primary school children and 38 university students. The procedure included baseline recording, a passive listening task, instruction, and an active listening task. Significant differences in skin conductance response (SCR) were found among the three age groups. The SCR became smaller for the groups of increasing ages only for the active listening task. As the trials progressed, there was a trend in the three age groups for decreasing SCR amplitude during the passive listening task.

Adolescent↗
PubMed1993

Presence of telomeric sequences on deleted chromosomes and their absence on double minutes in cell line HL-60.

The issue of telomeric sequences on deleted chromosomes and double minutes (dmin) was investigated by examining the cell line HL-60 with fluorescence in situ hybridization using a human plasmid DNA sequence with 800 bp TTAGGG repeats. This cell line showed telomeric sequences on the deleted short arms of chromosomes 9 and 10, with the results suggesting that so-called terminal deletion may be, in fact, an interstitial deletion, or that telomeric sequences may be synthesized by telomerase after deletion. On the other hand, numerous dmin showed no evidence of hybridization with the telomeric probe. This suggests that the characteristics unequal distribution of dmin during mitosis may result from the lack of not only centromeres but also telomeres.

Chromosome Deletion↗
PubMed1993

Effect of a peptide leukotriene receptor antagonist, ONO-1078, on guinea-pig models of asthma.

Peptide leukotrienes have been suggested to play an important role in bronchial asthma. As antigen-induced bronchoconstrictions, airway hyperreactivity, and pulmonary eosinophil accumulation are characteristics of the pathology of asthma, we investigated the effect of a peptide leukotriene receptor antagonist, ONO-1078, on these responses using guinea-pig models of asthma. Oral administration of ONO-1078 (3 mg/kg) significantly inhibited slow-reacting substance of anaphylaxis-mediated bronchoconstriction induced by i.v. administered ovalbumin. ONO-1078 (30-100 mg/kg), when administered orally both 1 h before and 4 h after ovalbumin challenge, significantly reduced immediate- and late-phase asthmatic responses, with peak responses occurring immediately and 5-11 h after challenge with inhaled ovalbumin. Oral administration of ONO-1078 significantly reduced the airway hyperreactivity (10-30 mg/kg) and the pulmonary eosinophil accumulation (30-100 mg/kg) observed 4 and 24 h after ovalbumin challenge, respectively. These results suggest that ONO-1078 may be of therapeutic use for bronchial asthma.

Acetylcholine↗
PubMed1993

Natural evolution of coronavirus defective-interfering RNA involves RNA recombination.

Defective-interfering (DI) RNAs of RNA viruses, in general, are generated and continue to evolve in size during serial undiluted passages of viruses. This evolution was thought to occur by independent generation of DI RNAs during virus passages and subsequent selection of new DI RNAs under new cellular conditions. Here we demonstrate that recombination between the old DI RNA and the helper viral RNA can be one of the mechanisms for natural DI RNA evolution. A mouse hepatitis virus (MHV) DI RNA, DIssE RNA, was transcribed in vitro and transfected into a mouse cell line infected with a different MHV strain (A59), which is distinguishable from the original natural helper MHV (JHM). During subsequent serial undiluted passages of the harvested virus, several novel DI RNA species were generated, while the original DIssE RNA disappeared by passage 11. cDNA cloning and sequence analysis of one of these novel DI RNAs, designated DI-2, revealed that it is composed of four discontinuous regions of the genomic sequence and is different from the structure of the original DIssE RNA. Sequence comparison among DI-2, DIssE, and helper MHV-A59 RNAs showed that DI-2 sequence is similar to DIssE in the first and second regions, but similar to the helper A59 virus in the third and last regions. Thus, this DI RNA was generated by RNA recombination between the original DIssE RNA and the helper viral RNA. These results indicate that recombination between DI RNA and helper virus RNA can be involved in the natural evolution of DI RNAs.

Animals↗
PubMed1993

Localization of the viral antigen of feline immunodeficiency virus in the lymph nodes of cats at the early stage of infection.

Immunohistochemical examinations of localization of feline immunodeficiency virus (FIV) Gag protein were performed on lymph nodes of cats experimentally inoculated with three different strains of FIV (infectious molecular clone of TM 1, Petaluma, and KYO-1 strains), using rabbit anti-FIV Gag serum. The FIV Gag antigens were observed in many follicular dendritic cells (FDCs) and sparsely in small lymphocytes of paracortical area in the lymph nodes of cats inoculated with Petaluma and KYO-1 strains. However, the antigens were present only in small lymphocytes, and not in FDCs of a cat inoculated with infectious molecular clone of the TM1 strain. The cell type differences in expression of the viral antigen in vivo might reflect on the cell tropisms of the FIV strains in vitro. By double immunohistochemical staining with rabbit anti-FIV Gag serum and monoclonal antibodies which recognize feline CD4, feline CD8 or feline pan-T molecules, the FIV Gag-positive lymphocytes were characterized as feline CD4-positive T cells. Since the distributions of FIV Gag antigens were mainly in the FDCs, the FDCs may play an important role as a major reservoir and may be a primary target of FIV at early stages of infection.

Animals↗