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Biomedical subjects

T Furuya

Publications and source records attributed to T Furuya.

At least 271 records · Page 15Linked to original sources
PubMed1983

[Application of an automatic blood cell counter, MICROX, to experimental animals. II. Leukocyte differential count in rabbits, rats, and mice].

Automatic blood cell analyzer "MICROX" was examined for possible applicability to the differential count of white blood cell in the rabbit, rat and mouse. Spun blood smear slides prepared from 37 rabbits were treated with Wright-Giemsa stain, and those from 49 rats and 46 mice were stained with Wright's dye. Totals of 4272 white blood cells of rabbit, 5484 cells of rat and 5329 cells of mouse were counted, respectively. The identification rate of MICROX was checked by the cell-by-cell counting method. Analysis of the data was made to examine correlation between the results with a MICROX and those of visual counting by optical microscopy on the same slides. 1) Corrected identification rates were 98.0%, 98.2% and 97.8% respectively in rabbits, rats and mice. 2) A high degree of correlation was observed between the results by MICROX and by optical microscopy in respect of segmented neutrophils and lymphocytes. 3) There were an average of 13.2 cells counted as unknown cells in rabbit blood, 10.5 in rat blood and 13.6 in mouse blood per 100 cells, respectively. Of the unknown cells about 30 to 40% were closely disposed leukocytes within the same counting squares. Almost all of atypical lymphocytes and erythroblasts were also counted as unknown cells on the analyzer. The data support the feasibility of the use of MICROX for the differential count of white blood cells of normal rabbits, rats and mice as well as monkeys and dogs.

Animals↗
PubMed1982

Direct effect of melatonin on the accessory sexual organs in pinealectomized male rats kept in constant darkness.

Pinealectomized and sham-pinealectomized male rats were subcutaneously implanted with 2 cm silicone elastomer capsules filled with testosterone or dihydrotestosterone (DHT) and placed in constant darkness (DD) for 50 days. The data revealed that the sham-pinealectomized group treated with testosterone differed from the pinealectomized group, having lower weights of accessory sexual organs and levator ani muscle. Pinealectomy had no effect on organ and muscle weights of DHT-treated animals. Exposure of male rats to DD resulted in a marked decrease in weights of ventral prostate, seminal vesicle, coagulating gland and levator ani muscle and a decrease in plasma DHT levels. However there was no significant reduction in plasma LH, FSH or testosterone. Pinealectomy of the rats exposed to DD resulted in restoration of both DHT levels and accessory sex organ weights. Melatonin implants in pinealectomized males led to an increase in both testosterone and DHT levels, accompanied by a decrease in sexual organ weights. The data indicate that the anti-gonadal effect of the pineal gland cannot be completely mediated by melatonin and that melatonin and some unknown factors can act at the tissue level to reduce the size and function of the affected sexual organs.

Animals↗
PubMed1982

[Application of an automatic blood cell analyzer "MICROX" to the blood of experimental animals. I. Differential counts of white blood cells in monkeys and dogs].

Automatic blood cell analyzer "MICROX" was examined for possible applicability to the differential count of white blood cell in the monkey and in the dog. Spun blood smear slides prepared from 42 monkeys and 60 dogs were stained with Wright's dye and totals of 4971 white blood cells of the monkey and 7189 cells of the dog were counted, respectively. The identification rate of MICROX was checked by the cell by cell counting method on a monitor TV of the instrument. Analysis of the data was made to examine correlationship between the results of MICROX and of optical microscopy on the same slides. 1) Identification rates and misclassification rates were 97.0% and 3.0% respectively in monkeys and 96.4% and 3.6% in dogs. 2) A high degree of correlation was observed between the results by MICROX and by optical microscopy in respect of segmented neutrophils and lymphocytes. 3) There were an average of 15.5 cells counted as unknown cells in monkey blood and 16.5 in dog blood per 100 cells, respectively. Of the unknown cells about 40 to 50% were closely disposed leukocytes within the same counting squares and almost all of atypical lymphocytes and erythroblasts were also counted as unknown cells on the analyzer. The data support the feasibility of the use of MICROX for the differential count of white blood cells of normal monkeys and dogs.

Animals↗
PubMed1981

Influences of some endocrine glands and of hormone replacement on the porphyrins of the Harderian glands of mice.

There are marked sex differences in the Harderian gland of the C3H/He strain of mice. Female (but not male) glands contain large amounts of porphyrin, which are readily visible as solid depositions within the lumina. The histology and porphyrin content of the Harderian gland were examined in intact and in pregnant mice and in mice subjected to combinations of adrenalectomy, gonadectomy and administration of sex steroid hormones. In male mice, castration approximately doubled the amount of porphyrin in the Harderian gland. Castration plus adrenalectomy increased the levels over 30-fold, to levels similar to those found in female mice, although adrenalectomy alone produced no significant effect. Administration of testosterone to the male mice which had been castrated and adrenalectomized prevented the increases while progesterone treatment produced further increases in porphyrins. In intact females, the amount of porphyrin varied with the phase of the oestrous cycle; being lowest during metoestrus and highest during dioestrus. In ovariectomized-adrenalectomized females, the effects of administered sex hormones on the amount of porphyrin in the gland were the same as in males. In pregnant mice, the level was no significantly different from that in intact oestrous animals.

Adrenalectomy↗
PubMed1980

CArcinogenic activity of clivorine, a pyrrolizidine alkaloid isolated from Ligularia dentata.

The carcinogenic activity of clivorine, a pyrrolizidine alkaloid isolated from Ligularia dentata, was studied in inbred ACI rats. Twelve animals in the experimental group received a 0.005% solution of clivorine in drinking water for 340 days and survived beyond 440 days after the beginning of the experiment. Of this group, 8 developed tumors in the liver; 2 developed hemangioendothelial sarcomas, and 6 developed neoplastic nodules. The hemangioendothelial sarcoma showed metastasis in the lung of one rat. No tumors were observed in the liver of the control animals.

Animals↗
PubMed1980

Comparative blood values of Macaca mulatta and Macaca fascicularis.

Hematological and clinical biochemistry values of two species of monkeys (Macaca mulatta and Macaca fascicularis) were measured by using auto-analyzers. WBC and RBC counts of M. fascicularis were high and Hb, MCV, MCH and MCHC values of M. fascicularis were low in comparison with those of M. mulatta. Albumin and creatinine levels of M. fascicularis were lower than those of M. mulatta. Total protein and A1P values of female M. fascicularis were higher than those of female M. mulatta. Differences between the sexes were observed in MCV, inorganic phosphorus, total protein, albumin and creatinine in M. mulatta, whereas in M. fascicularis, such differences were demonostrated as to creatinine, phospholipids and triglycerides.

Animals↗
PubMed1979

Mutagenicity of pyrrolizidine alkaloids in the Salmonella/mammalian-microsome test.

The mutagenicities of 7 pyrrolizidine alkaloids to Salmonella typhimurium TA100 were demonstrated by a modified Ames's method. The pyrrolizidine alkaloids found to be mutagenic were clivorine, fukinotoxin, heliotrine, lasiocarpine, ligularidine, LX201 and senkirkine. Pre-incubation of these alkaloids with S9 mix and bacteria in a liquid medium was essential for demonstration of their mutagenicities.

Animals↗
PubMed1979

Induction of hepatic tumors in rats by senkirkine and symphytine.

The carcinogenicity of the pyrrolizidine alkaloids senkirkine and symphytine was studied in male inbred ACI rats. Animals were divided into 3 groups: Group I received ip injections of freshly prepared senkirkine at a dose of 10% of the median lethal dose (LD50) twice weekly for 4 weeks and then once a week for 52 weeks. Group II received ip injections of symphytine at a dose of 10% of the LD50 by the same injection schedule as in group I. The control group was given ip injections of a 0.9% NaCl solution following the same injection schedule as in experimental groups. All group I rats survived for more than 290 days after the start of injections, and 9 of 20 rats developed liver cell adenoma. All group II animals survived for more than 330 days after the start of injections. Of 20 rats, 4 had liver tumors, 3 had hemangioendothelial sarcomas, and 1 had liver cell adenoma. The hemangioendothelial sarcomas showed metastasis in the lungs of 2 rats. The control group had no liver tumors.

Animals↗