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Biomedical subjects

T Furuya

Publications and source records attributed to T Furuya.

At least 253 records · Page 14Linked to original sources
PubMed1986

[A case of ulcerative colitis relapsing at the defunctionalized colon after subtotal colectomy].

A 25-year-old female with left sided ulcerative colitis underwent subtotal colectomy, and colo-anal anastomosis with diverting ileostomy by combined abdomino-sacral approach on February 14, 1985. Immediately after the operation, bleeding from the anus occurred, and one month after the operation, endoscopic examination revealed the relapse of the ulcerative colitis in the remaining colon. It was recognized, that relapse had occurred postoperatively at the nonfunctioning colon, which had been free from the disease so long as eight years since the onset of the disease. Then the relapsed colon was removed, and ileoanal anastomosis with diverting ileostomy was performed, again by combined abdomino-sacral approach. The sacral approach offered an excellent operative field and ensured the anastomosis between the small intestine and the anus.

Adult↗
PubMed1985

In vitro establishment of human fibroblasts of lysosomal diseases, GM1-gangliosidosis and Sandhoff disease, by transformation with origin-minus SV40 DNA.

The permanent human cell lines preserving defects of lysosomal enzymes, GM1-1019-SV and SA-1077-SV, were established from the respective fibroblasts from patients with GM1-gangliosidosis and Sandhoff disease by transfection with replication origin-minus simian virus 40 DNA. These cells grow rapidly without entering senescence during more than 120 population doublings. The activity of beta-galactosidase in GM1-1019-SV and of beta-N-acetylhexosaminidase in SA-1077-SV was respectively 40- and 180-fold lower than that of normal fibroblasts.

Cell Line↗
PubMed1985

[Local irritation of isotonic acetic acid solutions of the rabbit M. vastus lateralis and confirmation of the injected sites].

One ml of 0.106%, 0.425% or 1.7% acetic acid (AA) solution, isotonic to physiological saline solution, was injected into the M. vastus lateralis of male Japanese white rabbits. Two, 7, 14 and 21 days after the injections, the rabbits were autopsied, and the removed muscles were observed macroscopically. After fixation with 10% neutral formalin, they were examined histologically. Hemorrhage and white degeneration of the muscles were grossly observed in 0.106%, 0.425% and 1.7% AA at 2 days. These lesions disappeared at 7 days and thereafter in 0.106% AA, and at 14 days and thereafter, except for one animal, in 0.425% AA. In 1.7% AA, these lesions were observed until 21 days, but gross hemorrhage began to recover at 21 days. The size of the lesion, which was expressed as a product of the major axis (mm) and minor axis (mm) of the lesion, showed reduction over time, especially in 1.7% AA. Histologically, swelling, necrosis and calcification of the muscle fibers and cell infiltration, hemorrhage and dilatation in the interstitium were observed after the injections of 0.106%, 0.425%, and 1.7% AA. At 7, 14 and 21 days, regeneration of the muscle fiber and fibrosis in the interstitium were observed. The injected sites were examined by injecting a chip of test-tube brush as a marker. In 20 (83%) of 24 cases, it was ascertained that the markers were injected into the M. vastus lateralis. The markers were found in the M. vastus intermedius in 3 out of 4 other cases and in the intermuscular tissue between the M. vastus lateralis and M. tensor fasciae latae in one case.

Acetates↗
PubMed1985

Genotoxicity of a variety of pyrrolizidine alkaloids in the hepatocyte primary culture-DNA repair test using rat, mouse, and hamster hepatocytes.

Seventeen pyrrolizidine alkaloids were studied with the hepatocyte primary culture-DNA repair test using rat hepatocytes. DNA repair synthesis was elicited by 15 alkaloids, including 11 of unknown carcinogenicity, i.e., senecionine, seneciphylline, jacobine, epoxyseneciphylline, senecicannabine, acetylfukinotoxin, syneilesine, dihydroclivorine, ligularidine, neoligularidine, and ligularizine. The positive results with these alkaloids of unknown carcinogenicity suggest that they are possibly genotoxic carcinogens. The two pyrrolizidine alkaloids that did not elicit DNA repair were retronecine which lacks a necic acid component and ligularinine which lacks the unsaturated double bond at the 1,2-position of the pyrrolizidine ring. Five pyrrolizidine alkaloids, retronecine, monocrotaline, seneciphylline, senkirkine, and clivorine, were also tested in the DNA repair test with hamster or mouse hepatocytes. These alkaloids, except retronecine, showed a positive response in the test with hamster hepatocytes, but in the test with mouse hepatocytes clivorine in addition to retronecine was also negative. The results indicate a species difference in liver bioactivation of pyrrolizidine alkaloids, implying that there could be species differences in their carcinogenic activities.

Animals↗
PubMed1984

Effect of mitomycin C on bone marrow cells in mice.

The total and differential cell counts of femoral bone marrow and peripheral reticulocyte counts were determined in male ddY mice up to day 11 after an intraperitoneal injection of 2 mg/kg of mitomycin C. Total marrow cell counts showed a minimum value on day 2 after the injection, and then recovered to the control level on day 7. In the differential cell counts, the minimum values were observed on day 1 for proerythroblasts and basophilic erythroblasts, on day 2 for polychromatic erythroblasts, and on day 4 for mature neutrophils. Recovery was seen from the 4th to 7th days after injection. Marrow lymphocytes showed a maximum value when the total counts were minimum. The peripheral reticulocytes almost disappeared 4 days after the injection, and recovered to the pretreatment level on day 11.

Animals↗
PubMed1983

Dissociation of estrous cycle and activity rhythm in rats.

When rats were kept in continuous light there was a time-lag between the onset of activity arrhythmia and that of persistent estrus. When animals showing both arrhythmias in continuous light were kept under a LD 21:3 photoperiod, the activity rhythm returned to normal but the estrous cycle did not.

Activity Cycles↗
PubMed1983

Clearance of materials from breakdown of uterine collagen in mice during postpartum involution.

The collagen content of the mouse uterus increases during pregnancy and rapidly decreases to the level of nulliparous mice within a few days post partum. Most of the hydroxyproline in the uterine collagen had disappeared by day 3 post partum. During postpartum involution the hydroxyproline concentration in both the serum and blood clot was not higher than in nulliparous mice. The total hydroxyproline content in the liver, spleen and thymus was significantly increased during postpartum involution. These results indicate that materials derived from the breakdown of uterine collagen are rapidly removed from the blood stream by reticuloendothelial clearance.

Animals↗