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Biomedical subjects

T Fukuda

Publications and source records attributed to T Fukuda.

At least 829 records · Page 46Linked to original sources
PubMed1989

Preliminary report of microsporidia in Simuliidae larvae from Argentina.

Amblyospora bracteata, Polydispyrenia simulii, and 3 other undescribed microsporidia are reported from 11 species of neotropical blackfly larvae collected from streams in Buenos Aires (eastern) and Neuquen (southwestern) provinces of Argentina. Preliminary light and electron microscopy studies indicate that 2 of the undescribed species belong in the family Thelohaniidae and the third species is placed in the family Caudosporidae. Ten species of Argentine blackflies are recorded as new host records for A. bracteata and P. simulii.

Animals↗
PubMed1989

[Three cases of food-dependent exercise-induced anaphylaxis].

A variety of systemic reactions associated with exercise are recognized. This article describes three cases of anaphylaxis induced by exercise within 2 hours after the ingestion of causative foods. Their symptoms were urticaria, angioedema, bronchial asthma, fall in blood pressure and syncope. Cases #1 and #3 showed exercise-induced anaphylaxis after ingestion of wheat. However, case #1 reacted only to toasted wheat. In case #2 the most likely responsible food was also wheat, although we could not definitely confirm this. Two subjects exhibited an elevation in plasma histamine level when symptoms developed. In addition, we observed inhibition of these symptoms by pretreatment with anti-allergic drugs. These observations suggest that type I allergic reaction is involved in the development of these symptoms. However, why exercise combined with ingestion of food is needed for this type of anaphylaxis still remains unknown.

Adult↗
PubMed1989

Anti-ischemic effect of the novel anti-ischemic agent 2-(4-(p-fluorobenzoyl)-piperidin-1-yl)-2'-acetonaphthone hydrochloride and possible mechanism of its action.

The protective effect of E-2001 (2-(4-(p-fluorobenzoyl)-piperidin-1-yl)-2'-acetonaphthone hydrochloride) was examined in various ischemic models, and the mechanisms of its action were investigated in vitro and in vivo. 1. Pretreatment with E-2001 ameliorated the degeneration of pyramidal neurons in the hippocampal CA1 sector following transient ischemia in Mongolian gerbils. 2. E-2001 improved stroke symptoms induced by permanent unilateral carotid artery ligation in gerbils. 3. E-2001 prolonged the survival time following permanent bilateral carotid artery ligation in gerbils and mice. E-2001 also prolonged the survival time following intravenous injection of KCN into mice. 4. E-2001 suppressed the high potassium-evoked release of glutamate from rat hippocampal slices. Furthermore, E-2001 prevented the excessive accumulation of extracellular glutamate induced by a brief ischemia in gerbils. 5. E-2001 exerted calcium antagonistic action, i.e., a relaxing effect on high potassium-induced contraction of rat aorta. 6. E-2001 exerted inhibitory action on the lipoperoxide production in vitro and in vivo, and exhibited a radical scavenging effect against O- and N-radicals. These results suggest that E-2001 might be effective as a novel anti-ischemic agent.

Animals↗
PubMed1989

[Effects of indomethacin on cellular resistance and synthesis of prostaglandins in epithelial cells isolated from rat gastric mucosa].

This study was done to test effects of indomethacin (IND) on cell damage and prostaglandin (PG) synthesis in mucosal epithelial cells isolated from rat stomach in vitro. IND caused the cell damage in a dose-related way at concentrations over 5 X 10(-4) M. This damage was inhibited by 16,16-dimethyl PGE2 (10(-6) M). IND abolished the synthesis of PGE2, PGI2, and TXA2 at the concentration of 10(-4) M at which IND alone did not cause cell damage. The cells treated with 10(-4) M IND were significantly susceptible to damage caused by 15% ethanol compared to the cells not treated with IND. 16,16-Dimethyl PGE2 also inhibited the damage caused by IND + ethanol. These results suggest that the IND-induced susceptibility of the cells to damage is related to PG deficiency.

16,16-Dimethylprostaglandin E2↗
PubMed1989

[The impedance diagram of the nitella].

From olden time the resistance and capacitance of the living body were measured by alternating current bridge and the impedance diagram was made by rend 1/omega c using orthogoual axises, and it showes an arch. But by this research the impedance diagram of the Nitella was a line those are measured by finite alternating current. Formerly the frequencies were from 0.2Kc to 20Kc, but by this research the capacitance are nothing at over 4Kc.

Electrophysiology↗
PubMed1989

[Effects of sulbactam/cefoperazone in patients with respiratory infections in aged and/or with underlying respiratory diseases].

Sulbactam/cefoperazone (SBT/CPZ), a new antibacterial drug, was administered to 14 cases with respiratory infections for a duration of 5 approximately 13 days at a daily dose of 4 g. Diagnoses of these patients were 7 respiratory tract infections, and 7 bronchopneumonias. The underlying diseases were chronic pulmonary emphysema in 6 cases, bronchial asthma in 2 cases, and one each of bronchiectasis, diffuse panbronchiolitis and lung cancer with bronchoesophageal fistula. All patients had underlying respiratory diseases and/or were more than 70 years old. The rate of clinical efficacy was 78.6%. The incidence of penicillinase production by isolated bacteria was 18.2% and that of cephalosporinase was 63.6%. SBT/CPZ was expected to be more effective than CPZ alone in 3 cases judging from the susceptibility of the bacterial strains concerned, to antibiotics. No side effects were observed. We conclude that SBT/CPZ is useful in the treatment of respiratory infections of patients of advanced age and/or with underlying respiratory diseases.

Aged↗
PubMed1989

[Protection by 16,16-dimethyl PGE2 of surface epithelial, parietal, and chief cells isolated from rat stomach].

Damage caused by ethanol and its prevention by 16,16-dimethyl PGE2 were tested in fractions rich in surface epithelial cells (F1), rich in chief cells (F2), and rich in parietal cells (F3) isolated from rat stomach in vitro. Ethanol caused cell damage in F1 in a dose-related way and the concentration of ethanol for induction of 50% cell damage was 14.93%. There was no difference in cell damage caused by 15% ethanol between the three different cell fractions. 16,16-Dimethyl PGE2 (10(-6)M) significantly inhibited the damage caused by 15% ethanol in all the three fractions. These results suggest that 16,16-dimethyl PGE2 has direct protective effect on gastric cells and this action is not limited to the specific cell type.

16,16-Dimethylprostaglandin E2↗
PubMed1989

Density heterogeneity of eosinophil leucocytes: induction of hypodense eosinophils by platelet-activating factor.

We have examined the induction of hypodense eosinophils by platelet-activating factor (PAF), a mediator which may be involved in eosinophil activation in allergic diseases. Guinea-pig eosinophils were incubated with buffer or PAF and applied to continuous Percoll density gradients. Cellular density ranged from 1.0142 to 1.1369 g/ml. Peak eosinophil density in control was 1.0887 +/- 0.0008 g/ml (mean +/- SEM), and 91.1 +/- 1.4% of eosinophils were distributed between 1.0810 and 1.1000 g/ml. Preincubation of eosinophils with PAF(10(-7) M) resulted in a time-dependent and non-cytolytic increase of the number of hypodense eosinophils, with peak densities after incubation for 1 hr and 2 hr of 1.0834 +/- 0.0014 (n = 4, P less than 0.05) and 1.0755 +/- 0.0007 g/ml (n = 6, P less than 0.01), respectively. After incubation for 2 hr, 82.0 +/- 4.9% (n = 6) eosinophils showed a density lower than 1.080 g/ml. Lyso-PAF, the inactive precursor and metabolite of PAF, at a concentration of 10(-7) M had no effect on cell density. The specific PAF receptor antagonist WEB 2086 (10(-6) M) inhibited the PAF-induced density shift by 87.0 +/- 5.3%. Our results demonstrate that a single mediator is able to induce the formation of hypodense eosinophils. We conclude that the appearance of hypodense eosinophils in allergic diseases such as asthma may occur, at least in part, in response to inflammatory mediators which activate these cells.

Animals↗
PubMed1989

[Megakaryocytic leukemia with thrombocytosis].

A 62-year-old man was admitted to our hospital with exertional dyspnea. On admission, neither hepatosplenomegaly nor lymphadenopathy were noted. Laboratory data revealed anemia (Hb, 4.8 g/dl), leukopenia (2,800 microliters) and a normal platelet count (21 X 10(4)/microliters). The immature blast cells in the peripheral blood were 15%, which increased to 32% during his clinical course. On cytochemical studies, the blast cells had no staining with peroxidase, alpha-naphthyl-butyrate esterase and PAS, although acid phosphatase was positive. More than 58% of the blasts were identified as being of megakaryocytic lineage by platelet peroxidase and by tests with monoclonal GP IIb/IIIa antibody. Bone marrow biopsy disclosed marked fibrosis. However, the patient constantly had normal counts of platelets ranging from 21 X 10(4) to 63 X 10(4)/microliters. This case provides evidence that the megakaryocytic leukemias can be categorized into two types, which are characterized by either undifferentiated or differentiated megakaryocytic leukemia cells.

Cell Differentiation↗
PubMed1989

[Bone scintigraphy of metastatic calcifications].

The bone scintigrams of 4 cases with metastatic calcification in the lung, stomach and myocardium were presented. These four cases were malignancy with hypercalcemia. Metastatic calcifications were not able to be detected by radiographs and CT. Therefore, bone scan seems to be the most useful method for detection of metastatic calcification.

Aged↗
PubMed1989

[Factor analysis of bone mineral dynamics in patients with renal osteodystrophy].

Remarkably high accumulation of Tc-99m-MDP is seen in the skull on bone scintigraphy of patients with renal osteodystrophy (ROD), especially with secondary hyperparathyroidism. For the quantitative evaluation, the Factor Analysis (FA) was used for the early phase of bone scan. Tc-99m-MDP (14.8 MBq/body weight kg) was injected as a bolus through the medial antecubital vein. Dynamic acquisition of 40 x 30 sec frames were performed in a 64 x 64 matrix. For pre-processing, nine points smoothing was carried out, and then the region of interest was set on the frontal image of the head for the FA. The FA was performed with an 8 x 8 sampling corresponding to 64 dixels from 4,096 dixels. Bone factor was clearly extracted by the FA. Then, two original parameters were calculated. One is the bone radionuclide uptake counts (Bu) which is the product of the total radionuclide counts of skull and the contribution ratio, the other is the uptake ratio (Br) derived by the time activity curve (physiological component) of the FA. These parameters of ROD were significantly different compared to those of controls. The FA seems to be useful in quantitative evaluation of bone mineral dynamics.

Adult↗
PubMed1989

[Treatment of the association of arteriovenous malformation and cerebral aneurysms].

The association of an arteriovenous malformation (AVM) and a cerebral aneurysm is not so rare. Many reports have analyzed its localized relationship and studied hemodynamically the development of the cerebral aneurysm. However its therapeutic measures have not been established so far. At this time, authors have experienced 9 cases of AVM associated with cerebral aneurysm, and have studied suitable therapeutic measures. All 9 cases developed with intracranial hemorrhage, and in 6 of 9 cases, cerebral aneurysm was recognized in the feeding arteries or feeding system of the AVM (Group I). In the other 3 cases, localization of cerebral aneurysm was hemodynamically considered to have less of a relationship with AVM (Group II). It was characteristic that cerebral aneurysm in Group I was multiple, and developed frequently in the peripheral regions of the cerebral artery, especially in feeding system of the AVM itself. An operation was carried out in all 9 cases, and in Group I, 2 of 6 cases were operated on in one stage. The other 4 cases because of difficulty of management in the same operative field, were divided into two stages. In cases with clearly hemorrhagic sources, operation was firstly performed on the hemorrhagic source, and its residual focus was operated on after several months. However, in cases with unknown hemorrhagic source, cerebral aneurysm existing on the proximal side was operated on firstly. On the other hand, in Group II, when the operation was performed at one or two stages, the hemorrhagic source was dealt with first.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1989

Effect of aztreonam on immunohistochemical localizations of cytochrome P-450 (M-1) in male rats.

The localization of sex-specific cytochrome P-450, P-450 (M-1), was investigated immunohistochemically in the liver of untreated- and Aztreonam-treated male rats. P-450 (M-1) was uniformly distributed in the liver lobule of untreated rats. By treatment with Aztreonam, P-450 (M-1) was decreased more in the periportal region rather than the pericentral region of the lobule. In addition, oxidation of testosterone and 1 alpha-hydroxycholecalciferol catalyzed by P-450 (M-1) was also decreased by Aztreonam administration.

Animals↗
PubMed1989

[Analysis of the glucocorticoid receptor of bovine tracheal smooth muscle].

Though the therapeutic efficacy of glucocorticoids on bronchial asthma has been attributed mainly to their anti-allergic and anti-inflammatory effects, the possibility of intervention of glucocorticoids in bronchial asthma might be an important parallel process. However, little is known about the mechanism of glucocorticoid action on tracheal muscle and there is insufficient basic information about the glucocorticoid receptor of tracheal muscle. Accordingly we set out to analyze the glucocorticoid receptor of bovine tracheal muscle. Bovine tracheal muscle was used because it is plentiful enough for biochemical analysis. Our results showed that a small amount (0.08 pmol/mg.protein) of glucocorticoid binder of high affinity (kd = 4.6 nM) was present in the cytosol of bovine bronchial muscle. The binder did not show any affinity for estradiol, dihydrotestosterone or andronsterone, but showed a low affinity for progesterone. Furthermore, the binder underwent salt-dependent variation of its molecular form (9.2S under low salt condition and 4.6S under high salt condition) typical of steroid receptors. These results indicated the presence of a glucocorticoid receptor in the tracheal muscle, suggesting that glucocorticoid acts on bronchial muscle in therapeutic practice on bronchial asthma.

Animals↗