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Biomedical subjects

T Fukaya

Publications and source records attributed to T Fukaya.

At least 145 records · Page 8Linked to original sources
PubMed1986

Mouse monoclonal antibody (FKH1) detecting human melanoma-associated antigens.

A mouse monoclonal antibody, FKH1, was produced to detect cytoplasmic melanoma-associated antigen. FKH1 was raised using cultured human melanoma cell line KHm-6 as an immunogen. Reactivity of this antibody was assessed by immunohistochemical techniques against cell lines and normal and neoplastic tissues. Positive reactions were seen against 5 human melanoma cell lines. It stained cytoplasm of melanoma cells in a diffuse and granular pattern in indirect immunofluorescence. Immunoelectron microscopy showed diffuse distribution of immunoreactant in the cytoplasm of KHm-1 cells excluding melanosomes and other organelles. Reactivity against frozen and alcohol-fixed, paraffin-embedded melanocytic tumors was also tested with IIF or indirect or avidin biotinylated horseradish peroxidase complex immunoperoxidase techniques. All cases of frozen sections from benign and malignant melanocytic tumors showed positive staining with FKH1. In fixed tissues, however, reactivity was 11 of 14 (79%) in malignant melanoma and 28 of 42 (67%) in other melanocytic tumors. FKH1 did not react against normal melanocytes and nonmelanocytic tumors except APUDoma and 2 glioblastoma cell lines. It failed to stain the B-16 mouse melanoma cell line, neuroblastoma cell line, breast carcinoma cell line, and T-cell lymphoma cell line. Normal human peripheral nerves were nonreactive with FKH1. In immunoelectroblot study, FKH1 bound with proteins having molecular weight of 71,000 and 55,000 extracted from KHm-6 cells. It was suggested that FKH1 is a useful monoclonal antibody in diagnostic study of human malignant melanoma specimens.

Animals↗
PubMed1985

Effects of age and luteinizing hormone antiserum on human chorionic gonadotropin-stimulated testosterone secretion in young male rats.

The steroidogenic capacity of young male rats of different ages was studied. Two days prior to sacrifice at 5, 10, 15, 20, 25 and 30 days of age, the rats in treatment groups were given intramuscularly either human chorionic gonadotropin (HCG) at 20 I.U. twice daily/rat or luteinizing hormone (LH) antiserum (AS) at 0.25 ml twice daily/rat. Either saline or normal sheep serum (NSS) was given to control rats. The serum and testicular testosterone concentrations in the control rats averaged 0.85 +/- 0.03 ng/ml and 1.35 +/- 0.06 ng/mg testicular protein, respectively. At day-15 the serum and testicular testosterone concentrations in the HCG-treated rats had significantly increased to 9.30 +/- 0.85 ng/ml and 11.92 ng/mg of testicular protein, respectively. At the same age, the HCG-induced higher levels of serum and testicular testosterone concentrations were significantly reduced to 2.80 +/- 0.70 ng/ml and 6.02 +/- 1.00 ng/mg protein by concomitant administration of LH/AS and HCG. Our results suggest that the testosterone production in response to HCG stimulation is age-related. It was also determined that neutralization of circulating gonadotropin in LH/AS-treated rats decreased the sensitivity of Leydig cells to gonadotropin stimulation. This in vivo model should provide an excellent opportunity for the investigation of the testicular function in developing young males.

Age Factors↗
PubMed1985

Gossypol in female fertility control: ovum implantation and early pregnancy inhibited in rats.

Intramuscular administration of gossypol to normally cycling female rats induced an irregularity of the cyclic pattern for as long as the treatment was continued. Furthermore, administration of gossypol from days 0 (day of sperm-positive vaginal smear) to 8 of pregnancy prevented the normal maintenance of pregnancy. Serum values of progesterone and estradiol 17 beta in gossypol-treated normally cycling and pregnant rats were significantly lower than the control levels. The supplement of a combination of exogenous progesterone and estradiol 17 beta eliminated the inhibitory effects of gossypol on ovum implantation and the maintenance of pregnancy. Our results indicate that gossypol may have some usefulness in female fertility control.

Animals↗
PubMed1985

Familial syringoma. Case history and application of monoclonal anti-eccrine gland antibodies.

We studied a family with dominantly inherited eruptive syringoma. The father and the older daughter had chest and neck as well as eyelid lesions. The chest lesions seemed to develop continuously by new formation of eccrine germlike budding from the epidermis. Monoclonal antikeratin antibody EKH4, which predominantly labels the basal layers of the epidermis, stained positively in the cordlike epithelial structure and peripheral cells of the walls of cystic lesions. Staining with EKH6, which recognizes normal eccrine secretory and ductal structures--particularly luminal borders--was positive along the luminal borders of the cystic lesions; staining with EKH5, which labels eccrine secretory portion, was entirely negative in the lesions. These findings further supported the theory that syringoma of the eyelids and eruptive syringoma, which mainly involves the anterior neck and upper chest, are essentially the same tumor and that these appendage tumors are of eccrine ductal differentiation.

Adenoma↗
PubMed1985

Taenia taeniaeformis: inhibition of rat testosterone production by excretory-secretory product of the cultured metacestode.

In 3- to 5-month-old male Sprague-Dawley rats infected with the hepatic metacestode, Taenia taeniaeformis, the serum testosterone level was significantly lower than in comparable uninfected controls. By transmission electron microscopy, testicular Leydig cells of infected rats had less smooth endoplasmic reticulum than control Leydig cells. Cultured metacestodes isolated from the hepatic cysts secreted or excreted substances into the incubation medium. The effect of the excretory-secretory product on testosterone concentration in the sera and testes of 15-day-old rats was examined. Subcutaneous injection of 50-200 micrograms of excretory-secretory product/0.1 ml saline/rat for 2 days significantly reduced human chorionic gonadotropin-stimulated serum and testicular testosterone concentrations. Furthermore, the effect of the excretory-secretory product on isolated rat Leydig cell testosterone production was examined. Rat Leydig cells produced testosterone in vitro and, in the presence of 50 IU human chorionic gonadotropin/ml incubation medium, they responded with approximately 100% increase in testosterone production. Addition of 2-10 micrograms excretory-secretory product protein/ml of culture medium significantly reduced the testosterone production by rat Leydig cells in vitro. These results indicate that excretory-secretory product of cultured T. taeniaeformis metacestodes has a direct inhibitory effect on Leydig cell testosterone production under stimulation with human chorionic gonadotropin.

Animals↗
PubMed1985

In vivo and in vitro inhibition of human chorionic gonadotropin-induced testosterone production in rat testis by bursa of fabricius extract.

A bursa of Fabricius homogenate extract (BHE) was used to investigate the endocrine regulation function of this avian organ. In vivo and in vitro results indicated that BHE inhibited human chorionic gonadotropin (HCG)-induced testosterone production by rat testes. Leydig cells from collagenase-dispersed rat testes, when treated with BHE, showed a dose-response related depression of testosterone production under HCG stimulation. Young male rats, injected simultaneously with BHE and HCG, failed to show the marked testosterone production peak observed in rats injected with HCG only. However, in vivo treatments with BHE, in the absence of HCG stimulation, did not inhibit basal testosterone production. These results indicate that the bursa of Fabricius produces an endocrine regulation factor that inhibits HCG-induced testosterone production both in vivo and in vitro. Further investigation is necessary to isolate the active factor and determine its mechanism of action.

Animals↗
PubMed1985

[Studies on the alteration of the unconjugated estriol level of the maternal vein after the onset of labor].

We measured maternal venous unconjugated estriol (UE3) levels in 244 cases (218 cases with labor and 26 without labor). We investigated the relationships between several obstetrical factors and UE3 levels. There was no significant difference between the group with labor and the group without labor. However, in multipara, the UE3 level fell gradually with prolonged duration of labor. There were significant positive correlations between the UE3 levels and birth weights (n = 82, r = 0.375, p less than 0.001), and placental weights (n = 82, r = 0.381, p less than 0.001) in multipara with labor. There was no significant difference according to sex between the UE3 levels of a mother carrying a male or female fetus. The UE3 levels of fetal distress cases (n = 30, mean +/- S.E. 20.0 +/- 8.6 ng/ml) were significantly (p less than 0.02) lower than those without fetal distress (n = 188, 24.5 +/- 9.7 ng/ml). These data suggest that after the onset of labor, maternal venous UE3 has some relationship to feto-placental function.

Body Weight↗
PubMed1984

Sex difference in the human peripheral plasma beta-endorphin and beta-lipotropin levels.

Human plasma beta-endorphin (beta-EP) and beta-lipotropin (beta-LPH) were measured simultaneously by radioimmunoassay in 4 women with regular menstrual cycle (4 follicular phase and 4 luteal phase), 5 normal men and 10 term fetuses. Mean plasma beta-EP level in the total samples of 8 women was 30.7 +/- 5.8 pg/ml (+/- SE), significantly (p less than 0.03) higher than the beta-EP level in men (12.2 +/- 2.0 pg/ml). There was no significant sex difference in fetal plasma beta-EP level. These data suggest that the existence of sex differences for beta-EP secretion and release, and opioid peptides secretion and release mechanism might change with sexual maturation, due to changing sex steroids secretion.

Adult↗
PubMed1984

Serum cortisol levels of maternal vein, umbilical artery, and umbilical vein classified by mode of delivery.

We measured maternal venous (MV), umbilical arterial (UA), and umbilical venous (UV) cortisol levels of 180 pregnancies at term. The cortisol level of MV was higher than that of UA and UV in all deliveries. The cortisol levels of three samples had no difference between the induced labor group and the spontaneous onset of labor group regardless of delivery mode. In spontaneous vaginal delivery the cortisol levels of three individual samples were significantly higher in primipara than in multipara. The positive correlations were observed in any modes of deliveries between the cortisol levels of MV and UA, and MV and UA and UV. These data suggest that the fetus may produce cortisol by itself and maternal cortisol may inflow to the fetus.

Breech Presentation↗
PubMed1984

Chondrosarcoma of the nasal septum: a report of an uncommon lesion.

Chondrosarcoma of the nasal septum is an uncommon lesion. Review of the English literature reveals only 14 cases. This paper reports an additional case observed in a Japanese series of seven cases. The clinical features are reviewed, and a most effective treatment with minimum side effects is organized by an interdisciplinary group of specialists in surgery, radiotherapy and regional chemotherapy.

Adolescent↗
PubMed1984

The human fetal prolactin and estradiol levels, and their correlationship.

Serum prolactin and estradiol-17 beta levels were determined in umbilical cord sera obtained from 33 preterm, 118 full-term and 11 anencephalic infants and in peripheral sera obtained from 5 neonates. Prolactin levels increased from mid-gestation toward term, and markedly decreased after birth. Those in anencephalic infants (253 +/- 86 ng/ml, mean +/- S.D.) were lower than in full-term infants (324 +/- 119 ng/ml), but the difference was not statistically significant. We also performed thyroid-releasing-hormone (TRH) (50-200 micrograms) test on 5 anencephalic infants, but significant changes of serum prolactin levels were not found. Estradiol-17 beta levels in anencephalic infants were significantly lower than those in full-term infants. There was a significant positive correlation between cord prolactin and estradiol-17 beta levels in full-term infants. These results suggest one of the control factors for human fetal pituitary prolactin secretion is estrogen, and hypothalamic factors are not so important.

Anencephaly↗
PubMed1984

Serum cortisol levels in maternal venous, umbilical arterial and umbilical venous blood at delivery in the cases of fetal distress.

We measured maternal venous (MV), umbilical arterial (UA) and umbilical venous (UV) cortisol levels in 180 term pregnancies to investigate the changes of these hormone levels in the cases of fetal distress and neonatal asphyxia. The cortisol levels in UA and UV blood in the fetal distress group were significantly higher than those in the non-fetal distress group among spontaneous vaginal delivery cases. There were significant positive correlations among the cortisol levels in MV and UA, MV and UV, and UA and UV blood, respectively. These data suggest that in the case of fetal distress or neonatal asphyxia, mother and fetus have responded independently to the stress of delivery, and that maternal cortisol might not strongly affect the fetal cortisol secretion.

Asphyxia Neonatorum↗
PubMed1984

Differential staining of cytoid bodies and skin-limited amyloids with monoclonal anti-keratin antibodies.

The authors have used 5 different monoclonal antikeratin antibodies to study the antigenic profiles of cytoid bodies and skin-limited amyloids. Monoclonal antibodies AE1 (which stains the basal cell layer in normal human epidermis), AE2 (suprabasal layers), AE3 (whole epidermis), EKH4 (lower 2-3 layers), and EKH1 (recognizes all classes of intermediate filaments) were used to stain frozen skin sections by the indirect immunofluorescent or indirect immunoperoxidase technique. Cytoid bodies in lichen planus (LP) and discoid lupus erythematosus (DLE) were strongly stained with AE1, AE3, EKH4, and EKH1 antibodies but were negative with AE2. In contrast, amyloids in lichen amyloidosus and macular amyloidosis were stained strongly with EKH4 but only weakly or not at all with AE1, AE2, AE3, and EKH1. Amyloid associated with epithelial tumors showed closer immunologic profiles to cytoid body. These findings suggest that epidermal keratins are the major precursor substance of skin-limited amyloids as well as cytoid bodies in LP and DLE. Sequential changes in antigenic profiles from basal cells to amyloids through cytoid bodies further suggest that cytoid bodies may represent one of the precursor substances of skin-limited amyloids.

Amyloid↗
PubMed1984

[Serum prolactin levels following abortion in normal and molar pregnancy].

The changes in serum specific hCG and prolactin levels were studied in 20 women after the evacuation of normal pregnancy at 5-10 weeks (group A) and 10 patients after the evacuation of molar pregnancy of 9-16 weeks (group B). The elimination of circulating hCG was faster in group A than in group B, and that in women after term delivery(group C)was faster than in both groups A and B. The half-life of the serum hCG level was 44.4 hrs in group A, 42.7hrs in group B, and 38.9hrs in group C, respectively. The mean level of serum prolactin in group A was 27.4ng/ml at 0 day, 64.5ng/ml at 4-7 days, and 31.9ng/ml at 13-15 days after evacuation, respectively. The surge was statistically significant. Though no regular tendency was found in the prolactin levels in group B, the levels were relatively higher than in group A and nonpregnant women. No correlation was found between hCG and prolactin levels in either group A or group B. The administration of drugs, such as methotrexate, did not affect the prolactin levels. On BBT, the resumption of ovulation tended to occur when the prolactin level, rather than the hCG level, returned to its normal range.

Abortion, Induced↗
PubMed1983

Epidermal growth factor stimulates phosphorylation of pig epidermal keratin protein.

Endogenous protein phosphorylation of pig epidermis by epidermal growth factor (EGF) was studied to elucidate biologic roles of EGF on epidermal cells. EGF stimulated phosphorylation of keratin proteins (Mr: 65,000, 60,000, 56,000, and 51,000) identified by the Ouchterlony immunodiffusion analysis, a low Mr protein (16,000 dalton) of the urea-SDS-mercaptoethanol soluble fraction, and a 30,000 dalton Tris-HCl soluble protein. The phosphorylated epidermal proteins such as keratin proteins and a 30,000 dalton protein of the Tris-HCl soluble fraction were slightly dephosphorylated following the addition of unlabeled phosphate. Anti-EGF serum eliminated the EGF-stimulated phosphorylation of keratin proteins, a low Mr protein, and a 30,000 dalton Tris-HCl soluble protein. The overall results indicate that EGF specifically stimulated phosphorylation of several epidermal proteins, one of which was keratin protein.

Animals↗