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Biomedical subjects

T Fujino

Publications and source records attributed to T Fujino.

At least 289 records · Page 16Linked to original sources
PubMed1989

[Somatic cell hybrids used in cytogenetic analysis of transformation mechanism of uterine endometrial cell].

6-Thioguanine and Neomycin resistant human endometrial carcinoma cells were established from parental HHUA 95 cells in the present study. The karyotype was 46,XX although chromosome abnormalities had been observed occasionally. Cell hybrids between 6-TGr neor 95 and rat immortalized, non-tumorigenic 3Y1 cells were formed to analyze for cosegregation of chromosomes and tumorigenicity. However, the morphology of 3 hybrid clones was a spindle form, similar to the 3Y1 cells. In spite of the consistent presence of human chromosomes, negative anchorage independent growth in these clones suggested that none of the chromosomes involved in fused cells was associated with tumorigenicity of endometrial carcinoma. Loci on a few human chromosomes of a normal cell cause suppression in several hybrid systems. Cell fusion between 6-TGr 95 and normal human fibroblasts (HF) was performed to confirm whether this is also true in endometrial carcinoma. Hybrid cells had four copies of homologous chromosomes (two from each of the parent cells). These had a polygonal appearance, being similar to the HHUA 95 cells. Both anchorage independence and tumorigenesis were negative. Those results suggested that malignant transformation of endometrial cells was the result of multiple genetic changes. In this respect, the loss of genes implicated in the suppression plays an important role in endometrial carcinogenesis.

Adenocarcinoma↗
PubMed1989

[Coronary arteriographic findings in the patients with atrial septal defect and pulmonary hypertension (ASD + PH)--compression of left main coronary artery by pulmonary trunk].

The characteristic narrowing of left main coronary artery (LMCA) was found in 44% of patients (pts) with atrial septal defect and pulmonary hypertension (ASD + PH). The cause of the narrowing is thought to be the compression by pulmonary trunk (PT). Cardiac catheterization and coronary arteriography (CAG) were performed in 38 pts with ASD ranging in age from 15 to 62 years. We defined abnormal narrowing as 50% or more stenosis of AHA classification. Sixteen pts (42%) had PH, and of these pts 7 show the abnormal narrowing of LMCA. (18% of all pts with ASD, 44% of pts with ASD + PH). They had no signs of syphilis or aortitis. Of the pts with PH, those with abnormal LMCA revealed higher pulmonary artery mean pressure than those with normal LMCA (43.6 +/- 17.3 and 27.1 +/- 5.5 mmHg respectively. p less than 0.01). Other parts of coronary arteries are intact in all pts. These findings suggest that the LMCA abnormality relates to PH. In all cases with LMCA abnormality the narrowing revealed some special features indicate the cause of narrowing is compression. First, the most severe part of narrowing was the coronary ostium, and severity reduced gradually as distal LMCA. Second, the narrowing was estimated most severely in the view of LAO 20, but almost normal in the view of RAO 30. This finding suggests the narrowing is ellipsoid. Third, the shape of LMCA changed in the different phase of cardiac cycle. In the systole, the cranial border of LMCA was convex, but in the diastole it was concave. This indicates LMCA was soft and compressed.(ABSTRACT TRUNCATED AT 250 WORDS)

Angina Pectoris↗
PubMed1989

[A human infection of the cestode, Diphyllobothrium nihonkaiense Yamane et al., 1986].

A mature cestode, obtained from a 49-year-old man in Fukuoka, southern Japan, was examined. The worm was about 70 cm in length without scolex. The largest segment was 1.8 x 0.8 cm. Cirrus sac lay at an obtuse angle (about 150 degrees) to seminal vesicle. The eggs were 62.06 +/- 9.40 x 43.33 +/- 6.43 microns and the shell thickness 1.0-1.5 microns, with round, shallow and sparsely distributed surface pits ranging 0.8-2.0 microns in diameter. The above features suggested that this worm was identified as the cestode, Diphyllobothrium nihonkaiense.

Animals↗
PubMed1988

Successful excision of a right ventricular fibroma associated with ventricular tachycardia. Report of a six year survival.

A right ventricular (RV) tumor manifested by ventricular tachycardia (VT) accompanied by syncopal attacks was found in a 14 year old boy by two-dimensional echocardiography. Surgery was performed on February 10, 1981, with the aid of a cardiopulmonary bypass. The tumor was completely removed as a mass from the anterolateral portion of the RV wall. The wall was closed directly without any patch. The mass was 60 gm in weight and 7 by 4 by 4 cm in size. Fibroma was diagnosed by pathological study. The patient is doing well 6 years postoperatively. We conclude that two-dimensional echocardiography should be used to exclude cardiac tumors such as fibroma in young patients who have VT.

Adolescent↗
PubMed1988

Electrocardiographic changes related to hypersecretion of catecholamine in a patient with fulminant hepatitis.

A 48 year-old-man, with fulminant hepatitis complicated with myocarditis was treated. Despite intensive care, he died of fulminant hepatitis associated with hepatitis B virus infection. Electrocardiography (ECG) showed myocardial infarction-like changes when he went into a deep coma. Microscopically, scattered foci of myocardial cell damage and cell death associated with clusters of inflammatory cells were present in the heart at autopsy. However, there were no findings related to myocardial infarction and staining for hepatitis B surface antigen and core antigen were nil. The concentration of plasma catecholamine was elevated concomitantly with high level of ECG changes. We consider that abnormal ECGs may reflect a hypersecretion of catecholamine and suggest that our patient had a catecholamine cardiopathy.

Catecholamines↗
PubMed1988

[Beta-endorphin-like immunoreactivity in peripheral blood, especially in erythrocytes during delivery].

Recently it was reported that beta-endorphin-like immunoreactivity (beta-ELI) was detectable not only in plasma, but also in erythrocytes. To assess the presence of beta-ELI in human erythrocytes and also to determine if erythrocyte-associated beta-ELI may be released into plasma in stress situations, beta-ELI concentrations in plasma and erythrocytes were measured in pairs in normal menstrual cyclic women, postmenopausal women and pregnant women during delivery. After separating from plasma, erythrocytes pellets were lysed by adding distilled water. Extraction of beta-ELI in plasma and RBC lysate was performed with a Sep-pack C18 cartridge (Waters Associates Inc.), and beta-ELI concentrations were measured with a New England Nuclear radioimmunoassay kit. The following results were obtained: 1) There certainly are two pools of beta-ELI; one in plasma, the other erythrocyte-associated. 2) During delivery beta-ELI concentrations in plasma showed a great change, but beta-ELI concentrations in erythrocytes remained almost unchanged. From these results it is unlikely that erythrocytes act as a peripheral reservoir of circulating beta-ELI.

Adult↗
PubMed1988

Ultrastructural localization of erythrocyte cytoskeletal and integral membrane proteins in Plasmodium falciparum-infected erythrocytes.

The distributions of ankyrin, spectrin, band 3, and glycophorin A were examined in Plasmodium falciparum-infected erythrocytes by immunoelectron microscopy to determine whether movement of parasite proteins and membrane vesicles between the parasitophorous vacuole membrane and erythrocyte surface membrane involves internalization of host membrane skeleton proteins. Monospecific rabbit antisera to spectrin, band 3 and ankyrin and a mouse monoclonal antibody to glycophorin A reacted with these erythrocyte proteins in infected and uninfected human erythrocytes by immunoblotting. Cross-reacting malarial proteins were not detected. The rabbit sera also failed to immunoprecipitate [3H]isoleucine labeled malarial proteins from Triton X-100 and sodium dodecyl sulfate (SDS) extracts of infected erythrocytes. These three antibodies as well as the monoclonal antibody to glycophorin A bound to the membrane skeleton of infected and uninfected erythrocytes. The parasitophorous vacuole membrane was devoid of bound antibody, a result indicating that this membrane contains little, if any, of these host membrane proteins. With ring-, trophozoite- and schizont-infected erythrocytes, spectrin, band 3 and glycophorin A were absent from intracellular membranes including Maurer's clefts and other vesicles in the erythrocyte cytoplasm. In contrast, Maurer's clefts were specifically labeled by anti-ankyrin antibody. There was a slight, corresponding decrease in labeling of the membrane skeleton of infected erythrocytes. A second, morphologically distinct population of circular, vesicle-like membranes in the erythrocyte cytoplasm was not labeled with anti-ankyrin antibody. We conclude that membrane movement between the host erythrocyte surface membrane and parasitophorous vacuole membrane involves preferential sorting of ankyrin into a subpopulation of cytoplasmic membranes.

Animals↗
PubMed1988

[Radical excision followed by reconstruction of recurrent cancers of the head and neck].

Various modalities of treatment for recurrent cancers of the head and neck were studied and discussed, focusing especially on radical excision followed by reconstruction. Thirty-two patients with recurrent cancer of the head and neck were given this treatment over a 12-year period. Pectoralis major myocutaneous flap was most commonly used for reconstruction, while free latissimus dorsi myocutaneous flap with microneurovascular anastomosis was used for total cheek defect after surgery for cancer of the maxillary sinus. Five-year survival was 62% in cases of radical excision followed by reconstruction. Simple resection of recurrent lesions was conducted in 36 cases and the 5-year survival was also 62%. Radical neck dissection was performed in 22 cases in which cervical node metastases developed after control of the primary tumor, and the 5-year survival was 52%. On the other hand, the 5-year survival was not obtained in cases treated with radiotherapy alone in this series, while the 5-year survival was only 1% in cases treated with chemotherapy alone. It was concluded that surgery was the most reliable treatment for recurrent cancers of the head and neck, and that radical excision followed by reconstruction played an important role as salvage surgery.

Adult↗
PubMed1987

Use of bromocriptine in the treatment of normoprolactinemic infertility.

The efficacy of bromocriptine therapy was studied in 84 normoprolactinemic infertile patients. Bromocriptine (2.5-5.0 mg/day) was given for at least 1 month. Bromocriptine therapy was effective in 54 of 84 cases (64%), including 6 of 15 (40%) cases with amenorrhea, 16 of 24 cases (67%) with anovulatory cycle, 9 of 15 cases (60%) with delayed ovulation and 23 of 30 cases (77%) with luteal phase defect. Sixteen cases were able to conceive with bromocriptine alone. Twenty-five patients who did not respond to bromocriptine were treated with a combination therapy consisting of bromocriptine and clomiphene. Of the 25 cases, 14 responded to the therapy, and 6 of them were able to conceive. The response of prolactin to domperidone (10 mg, i.v.) was significantly (p less than 0.01) higher in the group responding to bromocriptine than in the nonresponding group. These results indicate that the administration of bromocriptine is an effective therapy for patients with normoprolactinemic endocrine disorders, and that domperidone may be useful in selecting the candidates for bromocriptine therapy.

Adult↗
PubMed1987

Suppression of ventricular premature contractions possibly related to triggered activity by oral diltiazem and atenolol.

The clinical importance of triggered activity as a cause of arrhythmias is uncertain. We assumed that ventricular premature contractions (VPCs) caused by triggered activity could be increased at higher heart rates and be suppressed by calcium channel blockers and beta-adrenoceptor blocker. Thus, we evaluated VPC frequency as a function of underlying heart rate and examined the efficacy of diltiazem and atenolol on VPCs, using 24 hour ECG recording. Plots of VPC frequency vs. heart rate were made at 1-beat/min intervals for all heart rates recorded for at least 5 min during 24 hours. Diltiazem (90-180 mg/day) and atenolol (50 mg/day) were given orally for 4 weeks, respectively in 36 and 16 patients with VPCs of more than 2000/day. Patterns of relationship between VPC frequency and heart rate observed before diltiazem therapy included: an increase of VPCs at higher heart rates (positive correlation) in 16 patients, an increase at low heart rates and a decrease at high heart rates (bidirectional correlation) in 13 patients, an increase at low heart rates and flat curve at high heart rates (positive-flat correlation) in 5 patients, a linear decrease (negative correlation) in 1 patient, and flat curve (flat correlation) in 1 patient. The patterns of correlation in patients treated with atenolol were positive in 6, bidirectional in 7, positive-flat in 2 and negative in 1. Both drugs significantly reduced the VPC frequency per 24 hours for patients with a positive correlation (P group), but induced no significant change for those with the other patterns of correlation (NP group). At the 70% VPC suppression level, diltiazem was effective in 9 of 16 patients of P group and only 1 of 20 patients of NP group (p less than 0.01); atenolol was effective in 5 of 6 patients of P group and only 1 of 10 patients of NP group (p less than 0.05). Both drugs reduced the slope of a positive correlation. These results suggest that: VPCs which increase at higher heart rates may be related to triggered activity, and an evaluation of VPC frequency as a function of heart rate predicts the response of VPCs to diltiazem and atenolol, and probably to other calcium antagonists and beta blockers.

Administration, Oral↗
PubMed1987

Argentophilic and scanning electron microscopic observations of the tegumentary papillae of Echinostoma revolutum (Trematoda) cercariae.

Argentophilic staining and scanning electron microscopy (SEM) were used to study the tegumentary papillae of Echinostoma revolutum cercariae. The most abundant papilla was uniciliate and arranged bilaterally on the body and tail of the cercaria. The distribution of these papillae was similar to that seen in other 37-collar spined echinostome cercariae. SEM revealed a total of 18 multiciliate papillae--16 on the anterior end and 2 at the middle of the body. These papillae contained up to 18 cilia per papilla and have not been reported previously from any cercaria.

Animals↗
PubMed1987

Ultrastructural localization of protective and nonprotective Plasmodium falciparum proteins using serum samples from vaccinated Aotus monkeys.

Postembedding immunoelectron microscopy, using pooled serum samples from a recent vaccination experiment involving Aotus monkeys, was used to localize immune targets in Plasmodium falciparum-infected erythrocytes and free merozoites. Serum samples from Aotus monkeys, protected completely by immunization with the P. falciparum merozoite surface coat precursor protein, identified immune targets on the surface of free and intracellular merozoites as well as the cytoplasm, plasma membrane, and parasitophorous vacuole membrane of immature schizonts. Serum samples from unprotected monkeys, which had been immunized with a complex of 143-kDa, 132-kDa, and 102-kDa polypeptides reacted specifically with the rhoptries of immature schizonts and mature merozoites.

Animals↗
PubMed1987

[MTX resistant mechanisms in human choriocarcinoma cells].

Choriocarcinoma cells grown in the presence of MTX have developed resistance in two ways. The HCCM derived sublines (relatively high MTX resistant) produced enhanced levels of DHFR and had relatively unimpaired transport of MTX, though altered transport was the primary determinant of response in the CC1 derived sublines (low MTX resistant). Since the selection procedure used was identical, it was assumed that altered MTX transport was insufficient to account entirely for various degrees of resistance. Increased DHFR activity was necessary for the development of high MTX resistance. The overproduction of DHFR was the consequence of amplification of the DHFR gene sequence. The incidence of DMs in metaphases paralleled the degree of resistance. Since DMs were also present in the cells not showing DHFR gene amplification, mechanisms other than DHFR gene multiplication were responsible for the de novo synthesis of DMs.

Cells, Cultured↗