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Biomedical subjects

T Bando

Publications and source records attributed to T Bando.

At least 145 records · Page 8Linked to original sources
PubMed1985

Pupillary constriction evoked from the posterior medial lateral suprasylvian (PMLS) area in cats.

Pupillary constriction was evoked by systematic stimulation using a microelectrode in the upper medial bank of the middle suprasylvian sulcus in the parieto-occipital cortex of the cat. The pupillo-constrictor area corresponded to the rostral and middle parts of the posterior medial lateral suprasylvian (PMLS) area. This pupillo-constrictor area extended by 2-3 mm along the middle suprasylvian sulcus. It is suggested that this pupillo-constrictor area overlaps or lies in close proximity of a part of the region in PMLS area related to lens accommodation, in which unit activity temporally related to lens accommodation was recorded and from which lens accommodation was evoked by electric stimulation.

Accommodation, Ocular↗
PubMed1985

Expression of epidermal growth factor and transforming growth factor-beta in human salivary gland adenocarcinoma cell line.

Neoplastic intercalated duct cells that originated from a human submandibular salivary gland release transforming growth factor and human epidermal growth factor into serum-free medium. The transforming growth factor with the soft agar colony-forming activity when assayed in the presence of mouse epidermal growth factor on normal rat kidney clone 49F indicator cells, but without mitogenic action to quiescent 3T3 cells, does not compete with epidermal growth factor for receptor binding, is heat and acid resistant but trypsin and dithiothreitol sensitive, and therefore is of the transforming growth factor-beta class. The immunoreactive human epidermal growth factor is detected by radioimmunoassay on certain fractions obtained by the Bio-Gel P-60 chromatography of neoplastic epithelial duct cells originated from a human submandibular salivary gland-conditioned medium and is biologically very similar to mouse epidermal growth factor. Moreover, the presence of human epidermal growth factor in cultured neoplastic epithelial duct cells originated from a human submandibular salivary gland is demonstrated by the peroxidase:antiperoxidase method.

Adenocarcinoma↗
PubMed1984

Physiological identification of midbrain neurons related to lens accommodation in cats.

Single units were sought in the medial midbrain, whereas lens accommodation of the eye was monitored by using an infrared high-speed optometer. Nineteen of 135 units isolated were identified as accommodation-related units by two criteria: 1) they discharged in synchrony with spontaneously occurring accommodation, and b) the units were driven by stimulating the interpositus nucleus of the cerebellum. In eight other experiments, units were sought that were antidromically activated by stimulating parasympathetic preganglionic fibers to the ciliary ganglion. Eleven of 46 antidromically activated units were identified as accommodation-related units (accommodation-related oculomotor units). Accommodation-related oculomotor units were found in the medial midbrain within 1 mm of the midline. The majority of them were located lateral and dorsal to the Edinger-Westphal nucleus.

Accommodation, Ocular↗
PubMed1984

Cortical neurons related to lens accommodation in posterior lateral suprasylvian area in cats.

Cortical units were sought that discharged in temporal correlation with spontaneously occurring lens accommodation in the area surrounding the middle suprasylvian sulcus, between the stereotaxic coordinates A8.0 and P1.0, while monitoring lens accommodation by using an infrared optometer. Units were tentatively identified as accommodation related if their discharges were modulated before the onset times of lens accommodation. Forty-eight accommodation-related units were found. Modulation of discharges preceded the onset times of accommodation by 360 ms on the average. Most (95%) of these units were related to the increase in the refractive power of the lens. Antidromic activation from the dorsal midbrain was tested in 26 of 48 accommodation-related units. Fourteen (67%) units were antidromically activated from the superior colliculus and/or the pretectum. Nine (64%) of them were also activated antidromically from the lateral posterior nucleus of the thalamus. The location of these units were confirmed by histological reconstruction. They were found in the posterior medial and posterior lateral lateral suprasylvian (PMLS and PLLS) areas and in the transitional zone of PMLS to the suprasylvian gyrus, between stereotaxic coordinates A7.0 and A1.5.

Accommodation, Ocular↗
PubMed1984

[Pharmacokinetics and clinical studies of fosfomycin in bile duct infections].

Serum and bile levels of fosfomycin sodium (FOM-Na) were evaluated and the pharmacokinetic parameters were estimated in 10 patients with various forms of biliary drainage (PTCD or T-tube). Clinical trials of FOM-Na were also performed in another 11 patients with bile duct infections and the clinical efficacy was estimated. Mean serum level of FOM-Na after drip infusion of 2 g fosfomycin sodium was 145.43 micrograms/ml at 1 hour, meanwhile bile level was 31.49 micrograms/ml at 2 hours. Serum level of FOM-Na was interpreted by the pharmacokinetic analysis after the damping GAUSS-NEWTON method using the one-compartment open model and bile level of FOM-Na by the damping GAUSS-NEWTON method and deconvolution method. Mean Vd was 12.16 L/body and half-time of serum level was 1.75 hours. Lag-time of bile level was 0.9 hour, Tmax 2.01 hours. The clinical efficacy of FOM-Na was determined excellent without any adverse effect. FOM-Na was remarkably effective in 91% of the patients. Hence, the first choice of FOM-Na against bile duct infections could be recommended.

Aged↗
PubMed1983

Properties of cerebello-precerebellar reverberating circuits.

Intracellular recordings were made from neurons of the red nucleus (RN) in cats where the cerebellar cortical effects were removed by chronic ablation of the intermediate part of the anterior lobe of the cerebellum. A prolonged depolarization could be elicited by stimulating the nucleus interpositus (IP) of the cerebellum, nucleus reticularis tegmenti pontis (NRTP) and the nucleus reticularis paramedianus (PMRN). This prolonged depolarization was abolished after cooling the inferior and middle cerebellar peduncles and persisted after ablation of the cerebral sensorimotor cortex. The prolonged depolarization was also recorded intracellularly from IP neurons. It was concluded that the prolonged depolarization set up in RN neurons is due to the repetitive discharges of IP neurons which produces tonic bombardment onto RN cells. The mechanisms of the repetitive discharges of IP neurons are considered to be due to impulse reverberation via the IP. The dynamic properties of the reverberating circuits were characterized by regenerative behavior. Above and below threshold, there were two states, the excited state where many constituent neurons were active, and the resting state where all neurons were inactive. It was found that cats with chronically stimulated cerebral peduncle (CP), and tested in an acute experiment, showed sometimes effective for inducing the prolonged depolarization and repetitive discharges of RN neurons by stimulation of IP. The prolonged depolarization thus produced could be reversibly abolished by cooling the middle and inferior cerebellar peduncles. The possible constituent neurons of the reverberating circuits were investigated in light of previous physiological investigations of stimulating the NRTP, PMRN, nucleus reticularis lateralis (LRN), nucleus olivaris inferior (IO) and recording EPSPs in RN cells. The RN cells receive axon reflex activation from NRTP and PMRN, and disynaptic excitation from NRTP, PMRN, LRN and IO. Based on these and other available data, the components of the cerebello-precerebellar reverberating circuits are discussed.

Animals↗
PubMed1983

An intracellular study of the optic tectum of the carp in vitro.

Intracellular recordings were performed on the optic tectum of the carp in vitro. Thick brain sections were prepared and the optic tract was electrically stimulated. EPSPs of 72 cells in stratum fibrosum et griseum superficiale, stratum griseum centrale and stratum album centrale were examine. Monosynaptic EPSPs were recorded from 19 cells in stratum fibrosum et griseum centrale and stratum griseum centrale, which was consistent with anatomical findings.

Animals↗
PubMed1981

Long-lasting evoked potential and repetitive firing recorded from the carp optic tectum in Cl-deficient medium in vitro.

Evoked potentials of the carp optic tectum following optic tract stimulation were examined in vitro. When the standard medium was replaced by Cl-deficient medium, the amplitude of the potential was augmented and the time course was prolonged. Following these changes, a long-lasting negative potential appeared in the deep layers. The involvement of a reverberating circuit is discussed.

Animals↗
PubMed1981

Cortical neurons in and around the Clare-Bishop area related with lens accommodation in the cat.

Single-unit discharges in the cat Clare-Bishop area were correlated with spontaneous accommodation responses. No appreciable change was found in accommodation responses evoked by stimulating the Clare-Bishop area, when cerebellar outflow was blocked reversibly by cooling the superior cerebellar peduncle. It is suggested, therefore, that the Clare-Bishop area plays an important role in the lens accommodation system through a pathway independent from that of the cerebellum.

Accommodation, Ocular↗
PubMed1981

Mesencephalic neurons controlling lens accommodation in the cat.

Thirty units were found in the midbrain of the anesthetized cat which discharged in correlation with spontaneously occurring lens accommodation. The frequency of spike potentials increased before the onset of the accommodation response. Increased discharges were followed by a silent period. These units were driven orthodromically by stimulating the interpositus nucleus of the cerebellum and the posterior commissure. Eleven of these units were identified antidromically as parasympathetic oculomotor neurons.

Animals↗
PubMed1980

Rebound excitation and the rhythmic activity of the ventrobasal complex of the thalamus.

(1) Membrane potential changes of 18 thalamocortical relay (TCR) cells of the ventrobasal complex of the cat thalamus were recorded intracellularly during rhythmic thalamic activities under moderate barbiturate anesthesia. (2) A single cutaneous stimulus evoked an initial EPSP followed by a longlasting IPSP. On the late declining phase of the IPSP, clustered rapidly rising depolarizations (RDs) were seen to generate a burst of spike potentials. The cluster of depolarizations was often followed by an IPSP, and another cycle of IPSPs and RDs with bursts of spikes was repeated. Similar rhythmic activities of TCR cells and clusters of RDs were also evoked by a single cortical stimulus, Spontaneous occurence of RDs was observed. (3) The temporal correlation between the occurrence of RDs and that of the spike potentials was noted. (4) Chloride ions were injected into the TCR cell through the recording microelectrode to eliminate the membrane hyperpolarization which would initiate the postanodal exaltation. After inversion of the IPSPs, RDs remained in the similar phase of rebound excitation to that before the reversal of the IPSPs. (5) it is concluded that excitatory inputs to TCR cells play an important role for the generation of the rhythmic discharges of TCR cells.

Animals↗
PubMed1980

Excitatory synaptic potentials and morphological classification of tectal neurons of the frog.

Under electrical stimulation of the optic nerve, intracellular recording and staining techniques were applied to tectal neurons of the frog. In the first part of this study, two response types in 25 cells were examined. One type (type I; 21 cells) was composed of an EPSP followed by an IPSP. The other type (type II; 4 cells) was composed of two successive EPSPs followed by an IPSP. The initial EPSP of type II response was concluded to be monosynaptic, while the other ones were assumed to be disynaptic. These responses were produced by myelinated fibers. Under stronger stimulus intensities, a pair of depolarization and hyperpolarization appeared at longer latencies. In the later part of this study, 22 cells were stained with Procion Yellow. Neurons were classified into three morphological types, i.e. vertical type (V-type; 10 cells), multipolar type (MP-type; 11 cells) and small neuron (S-neuron; one cell). All V-type neurons gave rise to type I response, while either type I or type II responses were recorded from MP-type neurons.

Animals↗
PubMed1980

[Studies on toxicity of clobetasone-17-butyrate (II)--chronic toxicity in rats (author's transl)].

Chronic toxicity of clobetasone-17-butyrate, an anti-inflammatory corticosteroid, was investigated in rats. Subcutaneous administration with the drug at dose of 0.003, 0.01 and 0.03 mg/kg/day for three and six months induced no significant changes in the rats. At 0.1 and 0.3 mg/kg/day, however, some dose-dependent symptoms such as suppression of body weight gain, emaciation, regressive changes in adrenals, lymphatic and hematopoietic tissues, decrease in circulating white blood cell and lymphocyte counts, which have been known as toxic effects of synthetic corticosteroids, were induced. The results indicates that the maximum no-toxic dose of clobetasone-17 butyrate was 0.03 mg/kg/day on this experimental condition. In the recovery test for two months no significant differences in the treated rats from controls were found, suggesting that the toxic effects were reversible in the animals treated at 0.3 mg/kg/day and lower than that.

Adrenal Glands↗
PubMed1980

[Studies on toxicity of clobetasone-17-butyrate (I)--acute toxicity in mice and rats and subacute toxicity in rats (author's transl)].

Acute and subacute toxicities of clobetasone-17-butyrate, a new anti-inflammatory corticosteroid, were studied in mice and rats. In the acute toxicity tests intraperitoneal LD50 values of the drug were estimated to be around 5 g/kg for both sexes of mice, 1.51 g/kg for male and 1.66 g/kg for female rats. Subcutaneous and oral administration induced no fatal cases at dose of 3.6 (mice, s.c.), 2.6 (rats, s.c.) and 6.0 g/kg (mice and rats, p.o.). As for the toxic signs in both mice and rats after the i.p. and s.c. administrations, emaciation was marked, and atrophy of thymus, spleen and adrenals were observed. No marked symptoms, however, were induced in animals administered orally. In the subacute toxicity tests male and female rats were subcutaneously administered with the drug at daily doses of 0.01, 0.03, 0.1, 1.0, 10 and 100 mg/kg for one month. Dose dependent symptoms such as suppression in body weight gain, emaciation, regressive changes in adrenal, lymphatic and hematopoietic tissues, decrease in circulating white blood cell and lymphocyte counts, and increase in total cholesterol level of serum were induced in the rats administered at 0.1 mg/kg/day and more than that, indicating that the maximum nontoxic dose in this experimental condition was 0.03 mg/kg/day. In recovery tests it was observed that the rats, which had been administered with the drug at 1.0 mg/kg/day for one month, were almost normal two months after the final administration.

Administration, Oral↗