Search PubMed⌕ Search

Biomedical subjects

S Zimmermann

Publications and source records attributed to S Zimmermann.

At least 109 records · Page 6Linked to original sources
PubMed1989

Close association of verotoxin (Shiga-like toxin) production with enterohemolysin production in strains of Escherichia coli.

Sixty-four verotoxin-producing (VT+) Escherichia coli strains were analyzed for VT1- and VT2-specific DNA sequences and for production of hemolysin. Strains of human origin were of the following serotypes: O157:H7 or H-, O111:H8 or H-, O26:H11, O114:H4, and rough:H7. Strains of serotypes O157:H7, O113:H21, O116:H21, and rough:H- were from cattle, while those of serotype O139:K12:H1 were from pigs. All 64 isolates carried either VT1 or VT2 or both genes. Sixty of the strains (93.8%) were hemolytic (Hly+). The three O139:K12:H1 strains examined produced alpha-hemolysin, as shown by their reaction with the alpha-hemolysin-specific monoclonal antibody h2A and by DNA hybridization with an alpha-hly gene probe. The remaining 57 Hly+ strains (95%) produced a different type of hemolysin (enterohemolysin), which is genetically and serologically unrelated to alpha-hemolysin. The two types of hemolysin are further distinguished by the appearance of the lysis zone on blood agar and by the time interval for the detection of hemolysis. In contrast to alpha-hemolysin, enterohemolysin can be detected only on blood plates containing washed erythrocytes. The frequent association of enterohemolysin with verotoxin production (89%) makes it useful as an epidemiological marker for rapid and simple detection of potential VT+ E. coli.

Animals↗
PubMed1988

[The clinical use of polyvalent immunoglobulin preparations].

A short survey is given of the indication for the use of polyvalent human immunoglobulin (human gamma globulin). The effect for the prophylaxis in hepatitis A is ascertained. The indication in the primary antibody deficiency syndrome is obligatory. In the secondary antibody deficiency syndrome with a moderate decrease of the immunglobulins as a rule a substitution is not indicated. A general prophylaxis or adjuvant therapy in surgical interventions, in polychemotherapy and in the framework of an intensive therapy without presence of an immune defect is to be refused. If in individual cases in sepsis, burn diseases or therapy-refractory infections additionally immunoglobulins are given, this must be done in high dosage. The modulation of immune reactions in autoimmunopathies is a new indication. Hyperimmunoglobulin and monoclonal antibodies will in future determine the immunoglobulin therapy.

Autoimmune Diseases↗
PubMed1988

Two-dimensional NMR studies of the porcine muscle adenylate kinase.

Porcine adenylate kinase was subjected to one- and two-dimensional proton NMR studies in order to identify amino acid spin systems and obtain sequence-specific resonance assignments. With a combination of results from a map of side-chain distances resulting from the refined X-ray crystallographic data and nuclear Overhauser effect spectroscopy (NOESY), assignments are suggested for all the aromatic spin systems.

Adenylate Kinase↗
PubMed1988

Enterohemolysin, a new type of hemolysin produced by some strains of enteropathogenic E. coli (EPEC).

42 Escherichia coli O26 strains which had been isolated at geographically different places and over a long time period were examined for hemolysin synthesis. 17 of these were found to be hemolysin-negative, nine strains were found to produce plasmid encoded alpha-hemolysin and 16 strains were shown to produce a phenotypically different hemolysin. This new type of hemolysin was called enterohemolysin and found to be genetically and immunologically non-related with the already described E. coli alpha-hemolysin. Enterohemolytic E. coli were not found in feces of 200 healthy infants under the age of two years. However, four E. coli O111 strains, one O121:H- and one O25:K5:H- strain, all from infants with diarrhoea were also enterohemolysin producers.

Animals↗
PubMed1987

[Variation in nocturnal blood pressure behavior in stage III (WHO) hypertension--a case report].

In some cases of hypertensive men the authors obtained 24-hour blood pressure profiles by easy non-invasive technique (Mercury manometer) and under the usual atmosphere and activities of a hospital. The control Group, 32 normotensive men, showed a normal circadian blood pressure variability with systolic acrophases at 10.00 h and 16.00 h and a bathyphase around 3.00 h. The diastolic blood pressure had a smaller variability. One patient with essential hypertension stage II (WHO), case 1, kept the normal day-night rhythm. By case 2, a man with essential hypertension stage III (WHO), the authors refer to the possibility of considerable differences of blood pressure behaviour during the night in cases of severe hypertension in contrary to normal blood pressure variability. After a supplementary fourth medication this patient showed a significant decrease of blood pressure during the night. Nevertheless he kept his acrophase at night. The recording of day-night profiles of blood pressure seems to be useful in relation to the judgement of classification of severity, of the mode of anti-hypertensive medication and of the success in therapy.

Antihypertensive Agents↗
PubMed1986

Characterization of hemolytic strains of Escherichia coli belonging to classical enteropathogenic O-serogroups.

147 isolates of enteropathogenic E. coli (EPEC) belonging to eleven different O-serogroups were examined for hemolysin-synthesis. Hemolysin-producing (Hly+) strains were found at high frequency in O-serogroups 26 and 114 and at low frequency in O-serogroup 126. The hemolytic activities of EPEC strains of different serogroups were found to be low when compared to Hly+ non-EPEC E. coli strains of different origins. Similarities between the hly determinants of EPEC strains and the hly region of an uropathogenic strain were detected by DNA-hybridization. By this method hly genes of EPEC strains belonging to O-serogroups 114 and 126 were localized on the bacterial chromosome. The hly determinants of O26 strains were localized on 100 +/- 5 MD size conjugative plasmids. The hly plasmid pEO5 of the O26 strain C4115 was transferred by conjugation into an E. coli K12 recipient and found to be stable inherited. The hemolytic activity of the transconjugant was comparable to the wildtype O26 donor. The plasmid pEO5 was found to be different in size, compatibility and hemolysin expression from a number of described hemolysin-plasmids.

Child↗
PubMed1986

[Immunomodulation with apheresis technics].

The use of apheresis therapy (plasmapheresis/plasmafiltration or cytopheresis) was successful in numerous immunological diseases. Autoimmune and immune complex diseases as well as paraproteinemias with hyperviscosity syndrome (M. Waldenström, plasmocytoma) are the main indications. The several modifications of apheresis and its indications are outlined, and possible immune regulatoric effects of apheresis therapy are discussed. Finally this paper refers to beginning changes from unspecific to more selective methods of apheresis (cascade filtration, cryofiltration, immunoadsorption, enzymatic degradation, continuous electrophoresis) and its significance for clinical and experimental immunology.

Adjuvants, Immunologic↗
PubMed1985

[Principles of cooperation between the specialties of internal medicine, pathology and clinical biochemistry].

The optimal utilization of the knowledge and possibilities of pathological and clinical biochemistry presumes a close cooperation between it and the clinical specialties. The common working team of the GDR Society of Internal Medicine and the GDR Society for Clinical Chemistry and Laboratory Diagnostics makes theses of the central points of the cooperation in care, education, further education and postgraduate study and in research a subject for discussion. As essential tasks in the process of medical care are regarded the balance of the examination programme standing at the disposal, the establishment of diagnostic programmes, the establishment of organisational measures, the ascertainment of a use according to indication, the guarantee of the representance of examination material, the control of plausibility and the interpretation of test results. Since the realization of the tasks to a large extent depends on the cooperation of the specialities in education, further education and postgraduate study during the further education the clinician should become acquainted with the possibilities, the limits and the prerequisites for the performance of laboratory diagnostic investigations, the clinical biochemist with the problems of medical care and the value of the laboratory diagnosis in the total process of the treatment. In the field of research the result is a necessary cooperation in the clarification of patho-biochemical mechanisms, in the search for suitable laboratory diagnostic parameters for diagnostics and control of the course as well as in the statement of the validity of laboratory diagnostic parameters and parameter combinations taking into consideration the factors expenses, benefit and risk as well as further diagnostic possibilities.

Biochemistry↗
PubMed1985

[Improved use of laboratory diagnostic tests].

The effective use of laboratory results is less effected by improvement of the quality of analyses than by standardization and qualification of the pre-analysis as well as by improvement of the diagnostic value (interpretation). The predictive value of a laboratory result of constand sensitivity and specifity is established by the prevalence of the disease in the population group examined. By preceding general examinations (anamnesis, clinical examination) the prevalence and thus the predictive value is increased. Permanent exchange of information and experiences between physicians and laboratory scientists is an essential contribution to the improvement of medical care.

Clinical Laboratory Techniques↗
PubMed1985

[Problem oriented diagnosis].

The development of modern diagnostics apart from the enlargement and deepening also led to a desintegration of the diagnostic. Indication and judgement are often uncritically performed. From this results the demand of a more problem-oriented diagnostics. As essential elements are regarded: the establishment and formulation of the problems - problem is all what needs diagnostic clarification or therapeutic management -, statement of the order of their solution, plan for the solution of each individual problems, control and correction, respectively of the way of solution.

Diagnosis↗
PubMed1984

[Plasma exchange therapy (plasmapheresis/plasmafiltration). II. Indications and complications].

On the basis of an extensive study of references as well as of own experiences the present state of the plasma exchange therapy, its success, problems and tendencies are represented. In the 1st part the essential modifications of the technique (plasmapheresis/plasma filtration), problems of anticoagulation, kind and quantity of the substitution as well as tendencies to more specific separation methods are explained. The 2nd part gives a survey of all essential indications (Goodpasture's syndrome, myasthenia gravis, hyperviscosity syndrome, lupus erythematodes visceralis, intoxications) and shows the main complications.

Connective Tissue Diseases↗
PubMed1984

[Plasma exchange therapy (plasmapheresis/plasma filtration). I. Technic].

With the help of an extensive study of literature as well as of own experiences the technical aspects of the plasma exchange therapy are described. Issuing from their hitherto existing course of the development and corresponding to their present significance the essential signs of the nowadays usual modifications (plasmapheresis by means of sedimentation in transfusion containers or by means of blood cell separator as well as plasma filtration) are explained. The discussion of problems of anticoagulation, the kind and quantity of substitution solutions as well as of developmental tendencies to more specific separation techniques leads over to the survey of essential indication areas (part II).

Anticoagulants↗
PubMed1981

[Behavior of ambulatory patients concerning the taking of medicine].

In 296 patients from a rural district the behaviour concerning the intake of medicaments was observed. After prescription of riboflavin-tablets over a period of 10 days three times an examination of the urine for signs of fluorescence was performed. Apart from this in a consultation of the physician finishing the test a control of not consumed remedies was performed. Hypertensive patients and patients with acute disease took the medicaments prescribed by the physician very unreliably. On the other hand, patients in the postconvalescence phase after myocardial infarction showed an essentially better intake discipline. In the majority of cases the irregular intake of medicaments was not admitted. A one time consultation on necessity and performance of the medicamentous therapy clearly increased the intake reliability during the examination period in a part of the patients.

Adolescent↗
PubMed1981

[Immunologic aspects of glomerular kidney diseases].

The experiment is made to describe the present knowledge of immune pathogenesis of the glomerulonephritis and the therapeutic account resulting from this. On principle it is to be differentiated between basal membrane-antibody-glomerulonephritis, as it is to be found above all in Goodpastures syndrome, and the essentially more frequent immune complex nephritides. However, immune complexes lead to pathological effects only then, when the capacity of phagocytosis has been transgressed or a qualitative or quantitative reduction of the immune response at humoral and/or cellular level is existing. Therapeutic points of influence result from the removal of the antigen, the influence on the production of antibodies (immunosuppression, immunostimulation, induction of the immunotolerance) and the influence on the effector mechanisms by antiphlogistics.

Anti-Glomerular Basement Membrane Disease↗
PubMed1980

[The problem-oriented case report--a method for the improvement of clinical training].

Abroad in the clinical education of the students the problem-oriented case report after Weed stood the test as a new training principle. In contrast to the classical case report the complaints and findings are formulated as problems and summarized in a list of problems. As problem is regarded what needs a diagnostic or therapeutic solution or a change of the patient's behaviour. For solution of the problem in a second step, the plan, the establishment of the ways of solution for every problem is done. In the notices of the course, the third part, the newly got findings are distributed to the adequate problems and their valency for the solution of the problem is tested. As advantages for the training are regarded: the establishment of the problems is more complete, the classification of psychic, social and health-educational problems is simpler. The physician is compelled to go over from the fixation on an individual problem to the valuation of all problems and to the correlations of several problems. Thus in the increasing specialisation an integrating role belongs to the problem-orientated case report. Independent formulation of the problems, development of the diagnostic and therapeutic steps, classification and valuation of their results in the notices of the course further the own responsibility of the student and renders possible a better control for the clinical instructor. The problem-oriented case report also forms the prerequisites for a problem-related letter of the physician.

Education, Medical↗