Biomedical subjects

S Zhang

Publications and source records attributed to S Zhang.

At least 559 records · Page 31Linked to original sources

PubMed1999

[Early use of Chinese drug rhodiola compound for patients with post-trauma and inflammation in prevention of ALI/ARDS].

OBJECTIVE: To evaluate the effects of the Chinese drug rhodiola on the preventive treatment of acute lung injury (ALI) caused by post-traumatic/inflammatory and thoracic-cardiovascular operations. METHODS: 104 patients who coincide with their diagnosis and standard scale were divided randomly in to single number groups A(1), B(1), C(1) and D(1) (early preventive treatment group EG) and double number groups A(2), B(2), C(2) and D(2) (control group CG). The treatment of primary disease both in EG and CG was similar, but in EG four grams of rhodiola compound (RCO) were used every day for a period of 3-15 days since admission and 5-7 days after operation. The indicators related to ALI in each groups were measured. RESULTS: PaO(2), AaDO(2) and hemorheology were significant in EG than in CG (P < 0.05) or (P < 0.01). The complication rate of ARDS was decreased about of 50.0% in EG than in CG. CONCLUSIONS: Early use of RCO may protect against major risk factors of ALI/ARDS caused by massive trauma/infection and thoracic-cardio-operations.

Adolescent↗

PubMed1999

[Correlation between gene expression of CCK-A receptor and gallbladder emptying in gallstone patients].

OBJECTIVE: To investigate the expression of CCK-A receptor and its relation with gallbladder hypomotility in patients with gallstone. METHODS: 20 patients with gallstone and 10 normal subjects were studied and gallbladder emptying function was measured by B ultrasonography. The other 8 patients without gallstone who died of accident were selected as controls for measuring mRNA expression of CCK-A receptor of gallbladder; RT-PCR was used to explore gene expression of CCK-A receptor of gallbladder. RESULTS: The gallbladder motility was significantly impaired in gallstone patients. The patients had lower mRNA expression of CCK-A receptor than control subjects (0.633 +/- 0.167 vs. 0.944 +/- 0.230, P < 0.01), and so did patients with impaired gallbladder motility then those with normal motility (0.544 +/- 0.124 vs. 0.768 +/- 0.131, P < 0.05). Furthermore, the change of expression of CCK-A receptors was markedly correlated with gallbladder emptying (r = 0.925, P < 0.01). CONCLUSIONS: mRNA expression of CCK-A receptor in gallstone patients decreases, which was related to gallbladder hypomotility. The down-regulation of gene expression of CCK-A receptor plays an important role in gallbladder hypomotility in patients with gallstone.

Cholecystolithiasis↗

PubMed1999

[Sex hormone receptor of hemangioma and vascular malformation in children].

OBJECTIVE: To investigate the level and difference of estrogen receptor (ER), progestogen receptor (PR) and androgen receptor (AR) in hemangioma tissues of Children. METHODS: 31 specimens of hemangioma and 36 specimens of vascular malformation were compound stained with hematoxylin-eosin and enzyme-linking affinitive immunohistochemistry. The prepared tissue slices were examined microscopically to find the ER, PR and AR in all the cells. RESULTS: The ER, PR and AR were detected in the two kinds of the tissue. However, their levels in the hemangioma were higher than those in the vascular malformation (P < 0.01) and the levels in the hemangioma of proliferating phase were higher than those in the hemangioma of regressive phase (P < 0.01). CONCLUSIONS: Hemangioma is one of the target tissues of estrogen. ER, PR and AR play an important role in the growth and development of hemangiomas. The binding site of the receptor may be occupied by extrogenous glucocorticoid so that hemangiomas gradually degenerates.

Adolescent↗

PubMed1999

[The diagnostic value of perforin and granzyme B genes mRNA expression in acute rejection after hamster to rat orthotopic liver transplantation].

OBJECTIVE: To explore the mRNA expression of perforin and granzyme B genes activated by cytotoxic T lymphocytes (CTL) for monitoring early acute rejection after liver transplantation. METHODS: The mRNA expression of perforin and granzyme B genes in grafts after gold hamster to rat liver transplantation was examined by reverse transcriptase polymerase chain reaction. Histological findings were studied comparatively, and isogenic rat liver transplantation was the control. RESULTS: The mRNA expression of perforin and granzyme B genes began to be up-regulated on the day 3 after transplantation, when no histologic evidence of rejection was found. Levels of expression significantly increased after day 4 and persisted through the whole course of rejection. In contrast, the control group showed no expression of mRNA for perforin and granzyme B. CONCLUSIONS: The determination of mRNA expression of perforin and granzyme B genes are useful to early diagnosis of acute rejection of liver transplantation.

Acute Disease↗

PubMed1999

[Microelectrode-guided posteroventral medial pallidotomy for Parkinson's disease].

OBJECTIVE: To introduce the methods and effects of microelectrode-guided posteroventral pallidotomy (PVP) for Parkinson's disease. METHODS: 109 patients underwent PVP by microelectrode electrophysiological recording for intraoperative target localization. 45 patients underwent unilateral PVP, 21 patients simultaneous bilateral PVP, 43 patients combined thalamotomy and PVP. Modified Webster Scale was used for objective assessments before and after operation. Postoperative CT scan or MRI was performed to localise lesions. RESULTS: Microelectrode recording usually led to a final pallidotomy lesion position that deviated from the CT stereotactically defined target point. The change rate of targets was 84.7%. PVP significantly and immediately improved all Parkinsonian motor signs and reduced drug-induced motor fluctuations and dyskinesia. The mean Webster Scale was improved by (72.7 +/- 11.3)% in the "on"" state, and (89.3 +/- 8.1)% in the "off" state. No patient showed permanent complications. CONCLUSIONS: Microelectrode-guided PVP was proved to be safe and effective. Bilateral PVP and combined PVP can significantly and immediately abolish all Parkinsonian motor signs. Physiological methods of microelectrode recording can significantly improve the safety and efficacy of PVP, and decrease the rate of complication.

Adult↗

PubMed1999

[Posttraumatic or inflammatory acute respiratory distress syndrome on high altitude area: a retrospective analysis of 94 deaths due to ARDS/MOF].

OBJECTIVE: To determine the characteristic predictors of acute respiratory distress syndrome (ARDS) in the high altitude area of China. METHODS: According to the diagnostic criteria of ARDS, 2 325 cases were screened in the recent 10 years. Except malignant tumors, cardio-cerebrovascular diseases, chronic obstructive pulmonary disease, and chronic multiple organ failure, the causes of death were analysed retrospectively. RESULTS: 94 cases (4.04%) were diagnosed as ARDS, but in the primary case record, only 27 cases (1.16%) had been diagnosed as ARDS/MOF. Of the 94 ARDS patients, 79 complicated by extrapulmonary organ damage involving the kidney, heart, liver, gastro-intestinal tract, and brain. A total of 305 organs were involved (average 3.24 organs each case). Lung failure only accounted for 15 cases. CONCLUSIONS: The combination of the diagnostic criteria of ARDS for high altitude area and flate area, injury severity scale, blood gas monitoring, and integrated traditional Chinese medicine and western medicine may improve the prognosis of ARDS.

Adolescent↗

PubMed1999

[Changes of PMN apoptosis in rabbits after burn].

OBJECTIVES: To study the dynamic changes of apoptosis of PMN from rabbits in the early stage after severe burn in vivo and investigate the effect of burn serum and wound exudate on PMN apoptosis in vitro. METHODS: 12 rabbits were randomly divided into 2 groups: control group (37 degrees C) and burn group (95 degrees C, 15 sec, III degrees 30% TBSA). Whole blood from the burn group at 0, 4, 8, 12, 24 h postburn was stained with acridine orange/ethidium bromide (AO + EB), and observed under UV microscopy. PMNs were isolated from both groups and cultured for 24 h (37 degrees C, 5% CO(2)) with normal serum (NS), burn serum (BS), and subeschar tissue fluid (STF) respectively. Morphological assessment and quantitation of apoptosis were performed with AO + EB stain and flow cytometric analysis. RESULTS: The number of apoptotic PMN in vivo decreased in the early stage postburn. In the BS + PMN group and STF + PMN group, the percentage of apoptotic PMN reduced (vs NS + PMN group, P < 0.05) and the specific DNA "ladder" pattern for apoptotic cells was not detected by agarose gel electrophoresis. We observed that both burn serum and STF could similarly suppressed PMN apoptosis while there was no significant difference between NS + normal PMN group and NS + burn PMN group. CONCLUSIONS: Apoptosis is inhibited in PMN from rabbits postburn in the early stage. Both burn serum and STF from rabbits postburn can inhibit PMN apoptosis in vitro.

Animals↗

PubMed1999

[Surgical treatment of traumatic retinal detachment with incarceration].

OBJECTIVE: To investigate the method and effect of vitreoretinal surgery for the traumatic retinal detachment with retinal incarceration. METHODS: 13 eyes of traumatic retinal detachment with incarceration were treated by vitrectomy, membrane peeling, relaxed retinotomy, endophotocoagulation, intraocular tamponade, and in some cases scleral buckling procedure. RESULTS: The retinae were attached in all the cases after operation. During the follow-up period of three months to three years, ten eyes kept the retina attached and three cases suffered from recurrent retinal detachment. One of them had had a second surgical procedure and obtained retinal re-attachment. The anatomic result was 11/13. The visual acuities were improved in all the patients. CONCLUSIONS: Traumatic retinal detachment with incarceration is often accompanied with severe proliferative vitreoretinopathy. Combination of vitreoretinal surgery is an effective method for the lesion. Relaxed retinotomy for the incarceration is the key procedure to obtain the operative success.

Adolescent↗

PubMed1999

[Data analysis on epidemiologic survey of cataract in China].

OBJECTIVE: To approach the prevalence of age-related cataract and its relationships with the condition of examination, diagnostic criteria and geographical environment in various areas. METHODS: According to the WHO's criteria of blindness and low vision, a statistical analysis of a national sample survey of blindness and low vision was carried out over the country. RESULTS: The number of patients with age-related cataract and visual acuity < 0.3 in both eyes calculated in the whole population was more than 5 million, the prevalence being 0.46%. The prevalence of blindness and that of low vision were 0.43% and 0.58% respectively. Among the blind, 41.06% was caused by cataract, and the low vision caused by cataract was 49.38%. Among elderly of >or= 60 years, the proportion of the blind and low vision caused by cataract was 73.13%. When the cataract standard was defined as lens opacity with visual acuity < 0.7, the prevalence of cataract was 5.99% in Beijing sample survey at the suburb area. The incidence of age-related cataract in females was significantly higher than that in males. In a survey of Beijing suburb in 1997, the prevalence of cataract among 5,084 elderly people of >or= 50 years with bilateral corrected visual acuity < 0.1 was 2.22%. In the southern part of China, the area of low latitude, especially in the plateau, such as in Guangdong Province (0.69%) and in Tibet (1.04%), the prevalence of cataract is much higher than that in northern part, high latitude areas, such as Heilongjiang Province (0.26%). A comparative study of the cataract prevalence of elderly >or= 40 years in the areas of different levels above the sea and sunshine times, it shows that the higher level above the sea and longer sunshine time the higher cataract prevalence. CONCLUSIONS: A longer sunshine time and a larger amount of ultraviolet irradiation are considered as the risk factors of cataract. Age-related cataract is the leading cause of blindness and the low vision in China.

Adolescent↗

PubMed1999

[Studies on DNA single strand breaks induced by sodium nitroprusside-nitric oxide donor].

OBJECTIVE: To study the effect of sodium nitroprusside (SNP), a nitric oxide (NO) donor, on DNA single strand breaks (SSBs). METHODS: A modified method was used to isolate and prepare the single cell suspension from the organs of mice. Alkaline single-cell gel electrophoresis (SCGE) was performed to examine DNA damage of the cells treated by SNP in vivo and in vitro. RESULTS: Treatment with 0.5 - 2.0 micromol/ml of SNP with S(9) for 1 h induced a concentration-dependent increase in DNA SSBs in g12 cells. Significant increase in DNA migration and comet frequency in the spleen, thymus and peritoneal macrophage were induced after intraperitoneal injection of SNP at a dose of 0.67 - 6.0 mg/kg. No obvious increase in DNA single strand breaks was observed in the liver, kidney and lung of the mice with same treatment. CONCLUSION: DNA SSBs could be induced by NO in some cells in vivo and in vitro. There was difference in sensitivity of various organs in the mice to NO. Cells of spleen and thymus and macrophage may be the important target cells of NO.

Animals↗

PubMed1999

[Epstein-Barr virus infection and expression of T-cell intracellular antigen-1 (TIA-1) in intestinal T-cell lymphoma].

OBJECTIVE: To study the status of Epstein-Barr virus (EBV) infection and the expression of TIA-1 antigen in 24 cases of intestinal T-cell lymphomas. METHODS: In situ hybridization for EBER1/2; immunohistochemical staining by three step ABC technique for TIA-1, LMP-1, CD30, CD45RO, CD3 AND CD20. 19 cases of intestinal B-cell lymphomas were used for comparison. RESULTS: (1) Of the 24 intestinal T-cell lymphoma cases, 23 were pleomorphic, medium to large cell type, and one cases was small cell type; in 20 of the 24 cases EBER1/2 signals were detected in the tumor cells; in 4 of the 20 EBER1/2 positive cases, tumor cells expressed LMP-1; EBER1/2 was not detected in 19 cases of intestinal B-cell lymphomas. (2) in 23 of the 24 cases, TIA-1 was expressed in most tumor cells, but not in the only case of small cell type; TIA-1 was negative in tumor cells of all 19 cases of intestinal B-cell lymphomas. CONCLUSIONS: A high level of EBV infection was present in this group of intestinal T-cell lymphomas, the expression of TIA-1 may be related to its cell origin. Intestinal T-cell lymphoma may be an EBV-associated extranodal NK/T cell lymphoma.

Adolescent↗

PubMed1999

[The significance of p16, cyclin D1 and pRb expression in soft tissue leiomyosarcoma].

OBJECTIVE: To evaluate the significance of p16, cyclin D1 and pRb expression in human soft tissue leiomyosarcoma (LMS) and the relationship among these three proteins. METHODS: 42 cases of LMS were studied for p16, cyclin D1 and pRb expression with immunohistochemical method. RESULTS: The total abnormality percentage of p16, cyclin D1 and pRb expression in all 42 cases of LMS was 97.6%. The negative rate of p16 and pRb protein expression was 35.7% and 47.6% respectively, and the overexpression rate of cyclin D1 was 57.1%. Overexpression of cyclin D1 was also noticed in 55% leiomyomas. In the 20 cases of LMS which were pRb negative, 19 cases were moderately or strongly positive and one faintly positive for p16. Contrastly, in the 15 cases of LMS negative to p16, 12 cases were moderately or strongly positive and 3 cases faintly positive for pRb. Higher rates of loss of pRb protein were observed in the low-differentiated group than in the high-differentiated one. CONCLUSIONS: (1) The abnormality of p16-cyclin-pRb pathway is highly related to the pathogenesis of LMS. (2) Loss of p16 or pRb and/or overexpression of cyclin D1 may be involved in the pathogenesis of LMS. (3) There is a negative reciprocity between p16 and pRb protein expression in LMS. (4) Overexpression of cyclin D1 is a common molecular alteration that may occur in the early stage. (5) Expression of pRb is inversely related to the differentiation degree of LMS. (6) The expression of the 3 proteins studied has no relation to tumor location, recurrence, invasion or metastasis.

Biomarkers, Tumor↗

PubMed1999

[Evaluation of therapeutic efficacy by detection of specific IgG and IgG4 against KLH in schistosomiasis japonica].

The specific IgG and IgG4 antibodies in the sera of patients with Schistosoma japonicum were determined by KLH-ELISA and SEA-ELISA. The results showed that KLH-ELISA was as sensitive and specific as SEA-ELISA. The negative conversion rates of IgG and IgG4 12 months after treatment detected by KLH-ELISA were significantly higher than those by SEA-ELISA. The negative conversion rate of IgG was 92.9%, and that of IgG4 was 97.6% 24 months after treatment. Therefore, it is regarded that detecting specific IgG and IgG4 by KLH has high value in both diagnosing schistosomiasis japonica and assessing its therapeutic efficacy. Particularly the specific IgG4 may be a short-duration antibody which can indicate successful chemotherapy of patients with chronic Schistosoma japonicum infection.

Animals↗

PubMed1999

[Protective effects of ischemic preconditioning on ischemia reperfusion injuries of kidney: experimental studies].

We studied the serum levels of SOD and creatinine(Cr) of venous blood and changes in renal tissue structures under electromicroscope in the ischemia referfusion(I/R), the ischemic preconditioning(PC1, PC2, PC3), and the control groups in rabbits. The results were that the serum levels of SOD and Cr in the I/R group were obviously higher than that in the PC and control groups after one-hour ischemia and one-hour reperfusion(P < 0.001). There were no significant difference in Cr levels between the PC2 and control groups; there were significant degenerate and necrotic changes under the electromicroscope in renal tissues in the I/R group, but there were no significant change in the PC and control groups. The results suggest that PC plays a role in protecting the kidney from injury induced by ischemia and reperfusion.

Animals↗

PubMed1999

[Relation of left ventricular mass and QT dispersion in patients with essential hypertension].

To study the relation between the rate-corrected QT interval dispersion(QTcd) and left ventricular mass, the blood pressure(BP), left ventricular mass index(LVMI), and QTcd were measured in 79 essential hypertensive patients. The results were that QTcd and QTcd% in hypertension with left ventricular hypertrophy(LVH) group were significantly higher than that in hypertension without LVH group; The higher the LVMI, the higher the QTcd and QTcd%(r = 0.7720, r = 0.6798, respectively), which were not associated with BP. The results suggest that LVH, but not hypertension, leads to an increase in QTcd.

Adult↗

PubMed1999

[GC-MS analysis of volatile constituents of rhizoma Heterosmilacis].

OBJECTIVE: To extract and analyze the volatile constituents from the dried rhizomes of Heterosmilax yunnanensis(A) and H. chinensis(B). METHODS: Qualitative and quantitative analysis were made by GC-MS and GC. RESULTS: Forty and forty-seven constituents were identified from A and B respectively, the identification rates being 74.85% and 81.26% respectively. CONCLUSION: The volatile constituents of A and B are almost the same, but slightly different in content.

Gas Chromatography-Mass Spectrometry↗

PubMed1999

[Determination of flavonoids in pollen typhae(Puhuang) by HPCE and HPLC].

OBJECTIVE: To determine two flavonoids isorhamnetin-3-O-neohesperidoside(I) and typheneoside(II) in Pollen Typhae by HPCE and HPLC. METHOD: HPCE conditions: buffer = 0.02 mol.L-1 borate, 0.05 mol.L-1 SDS and 10% acetonitrile; V = 20 kV; T = 30 degrees C; lambda = 270 nm; sample size = 30 kPa.s, HPLC conditions: C18 lumn, acetonitrile-water (15:85) as mobile phase detection wavelength at 254 nm. RESULT: The contents of flavonoids I and II in five different Typha species and eighteen commercial samples of traditional Chinese drug Puhuang were between 0.1%-0.6%. CONCLUSION: These two methods are rapid, simple, accurate and can be used for the quality control of this traditional Chinese drug.

Chromatography, High Pressure Liquid↗

PubMed1999

[Free radicals on cataracts induced by sodium selenite studied by ESR and LPO].

We made a electron spin resonance (ESR) study on rat cataracts induced by Na2SeO3. A signal was detected at about g = 2.0026, and the content of electron spins (CES) in lens of rat administrated Na2SeO3 for a short time is higher than that of control significantly. At the late stage of cataracts, the content of CES was lower than normal level. The results of kinetics analysis showed that the CES grow up with the administration of Na2SeO3 and decays in index-law after administration. The results suggested that free radical scavengers or antioxidants should be useful for preventing cataracts at initial stage of the oxidation damage to lens, but they could aggravate cataracts at late stage of cataracts. This can explain the controversy about the curative effect of antioxidant to cataracts.

Animals↗