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Biomedical subjects

S Yano

Publications and source records attributed to S Yano.

At least 559 records · Page 31Linked to original sources
PubMed1986

Some profiles of transmucosal potential difference in rat stomach determined in situ with special reference to effects of timoprazole, a H+,K+-ATPase inhibitor.

Gastric transmucosal potential difference (PD) is referred to an index of function and integrity of the mucosa. The features of gastric PD were studied in association with gastric acid secretion. The gastric PD was measured at the forestomach, glandular portion and pylorus in the rat in in situ preparations. Secretagogues such as bethanechol (BeCh, 50 micrograms/kg, i.v.), tetragastrin (TG, 30 micrograms/kg, i.v.), and histamine (Hist, 10 mg/kg, s.c.) produced a decrease in PD at the three regions of the stomach. These PD reductions did not occur with the combined treatment with timoprazole (30 mg/kg, i.d.); a marked increase in PD was noted, especially, in the case of Hist plus timoprazole. Similarly, BeCh induced PD decrease was antagonized by atropine (30 micrograms/kg, i.v.) and Hist induced PD decrease was attenuated by cimetidine (10 mg/kg, i.v.), while TG induced PD decrease was not affected by either of them. Of the antisecretory drugs, only cimetidine produced an increase in basal PD, probably via a mechanism unrelated to acid secretion. These results suggest that the PD decrease by each secretagogue seen at oxyntic and non-oxyntic gland regions of the stomach primarily originates from secretory activation of the parietal cells and that its action on function unrelated to acid secretion also exerts a minor influence on gastric PD.

2-Pyridinylmethylsulfinylbenzimidazoles↗
PubMed1986

Effects of interleukin-2, OK-432 and interferon-gamma on in vitro induction of nonspecific killer cells by concanavalin A in mice.

Human interleukin-2 (IL-2), OK-432 and mouse interferon-gamma (IFN-gamma) were evaluated for their beneficial effects on in vitro induction of nonspecific killer cells by concanavalin A (Con A). Nonspecific killer cells could be induced by culturing C57BL/6 spleen cells for 4 days with Con A. Con A activated killer cells showed direct cell-mediated cytotoxicity (DCMC) and lectin-dependent cell-mediated cytotoxicity (LDCC) against natural killer (NK) sensitive YAC-1 cells, and LDCC against NK-resistant EL-4 cells in a 4 hr-51Cr release assay. Higher DCMC and LDCC reactivity against YAC-1 cells, and higher LDCC reactivity against EL-4 cells were obtained with spleen cells cultured with Con A plus IL-2, or Con A plus OK-432 than with those cultured with Con A alone. No beneficial effect of IFN-gamma and no additive effects of IL-2 plus OK-432, or IL-2 plus IFN-gamma were observed in the induction of nonspecific killer cells by Con A.

Animals↗
PubMed1986

Immunosuppression by captopril in vitro: inhibition of human natural killer activity by copper-dependent generation of hydrogen peroxide.

The effect of captopril and a mixture of captopril and copper on natural killer (NK) activity of normal human peripheral blood mononuclear cells (PBMC) was examined. Preincubation of PBMC with captopril alone did not affect their NK activity at concentrations of 5-50 micrograms/ml. However, in the presence of copper sulfate, captopril inhibited the NK activity in a dose-response fashion. Similar inhibition was observed when adherent depleted fraction was treated with captopril and copper. In the time course study, significant inhibition of NK activity by captopril and copper was already observed at 3 hr preincubation. The inhibition of NK activity by captopril and copper was completely abolished by the addition of catalase, but not by superoxide dismutase, interleukin-2, or indomethacin. Preincubation of PBMC with captopril and copper for 18 hr decreased its viability. This decrease was also reversed in the presence of catalase. These results suggest that immunosuppression by captopril in the presence of copper was mediated by hydrogen peroxide.

Captopril↗
PubMed1986

Oral transmission of human T-cell leukemia virus type I in the rabbit.

Four female rabbits were given twice-weekly oral inoculation of 2-4 X 10(7) cells from a male rabbit lymphoid cell line persistently infected with human T-cell leukemia virus type I (HTLV-I). After 8 weeks, one of them was found to be seroconverted for HTLV-I. Peripheral lymphocytes from the 4 rabbits were cultured in the presence of T-cell growth factor, and a lymphoid cell line with a normal female karyotype was established only from the seroconverted rabbit. This cell line was reactive with a monoclonal antibody to rabbit T-cells and expressed HTLV-I antigens and virus particles.

Animals↗
PubMed1986

Isolation and characterization of rat nephritogenic and non nephritogenic brush border antigens.

Two kinds of brush border antigens were isolated from pronase-treated rat tubular material by gel filtration, DEAE-chromatography and disc-electrophoresis, successively. One, a 0.05 M antigen which was eluted from DEAE-column with 0.05 mol of NaCl solution, has no nephritogenic ability when inoculated into homologous rats. The other, a 0.30 M antigen eluted with 0.30 mol of NaCl solution, induces membranous nephritis when injected into rats. Immunoprecipitation studies show no common factor between these two antigens. SDS-polyacrylamide electrophoresis shows the molecular size of 0.05 M and 0.30 M antigens to be respectively over 200 kD and about 90 kD. Rabbit antiserum against the 0.05 M antigen fixed to the GBM in a diffuse granular fashion as well as to the brush border by immunofluorescence when incubated in vitro with normal rat kidney section. Rabbit antiserum to the 0.30 M antigen, however, fixed exclusively in vitro to the brush border. Passive transfer of nephritis was studied with these rabbit antisera. When antiserum to 0.05 M antigen was injected into normal rat, diffuse granular deposition of rabbit IgG was observed in the GBM within 2 h of the injection, but the deposits became negative 1 week later. Rats injected with antiserum to the 0.30 M antigen showed no glomerular deposition within 2 days but diffuse granular deposits of rabbit IgG were observed within 1 week and increased until 2 weeks after the injection. These facts should be considered in the studies on passive Heymann nephritis and its pathogenesis.

Animals↗
PubMed1986

Reduction of rat gastric transmucosal potential difference by indomethacin partly associated with its acid secretory activity.

Gastric transmucosal potential difference (PD) is referred to as an index of function and integrity of the mucosa. Gastric instillment of indomethacin for 30 min significantly reduces the PD (lumen negative to serosa) at the glandular portion of the rat stomach, but not at the forestomach. The PD reduction of the glandular portion disappeared by pretreatment with prostaglandin E2 (PGE2), although PGE2 alone produced an increase in PD. The PD decrease induced by indomethacin was greatly recovered by additional treatment with timoprazole, a H+,K+-ATPase inhibitor. Timoprazole alone did not influence gastric PD. These data suggest that indomethacin acutely reduces gastric PD in part through the acid secretory activity, apart from the damaging activity on the mucosa.

2-Pyridinylmethylsulfinylbenzimidazoles↗
PubMed1986

Right and left ventricular ejection patterns in D-transposition of the great arteries assessed by pulsed Doppler echocardiography.

Pulsed Doppler echocardiography (PDE) was performed to evaluate the flow velocity patterns of the right (RVOT) and left ventricular outflow tracts (LVOT) in 10 patients with transposition of the great arteries (D-TGA). Twenty normal children served as controls. Systolic time intervals (STIs) and acceleration times (AcT) of the right and left ventricles were measured using PDE. The following results were obtained. In normal controls, the right ventricular pre-ejection period (RPEP) was shorter than the left ventricular pre-ejection period (LPEP), and the right ventricular ejection time (RVET) was longer than the left ventricular ejection time (LVET). The mean RPEP/RVET was 0.33 (range 0.25-0.42) and the mean LPEP/LVET was 0.38 (range 0.28-0.55). In normal controls, the flow velocity pattern of the RVOT had a dome-like contour with a peak velocity in mid-systole, and the mean AcT/RVET ratio was 0.50 (range 0.41-0.62); that of the LVOT had a triangular shape with a mean AcT/LVET ratio of 0.31 (range 0.24-0.38). In patients with D-TGA, the RPEP was longer than the LPEP and the RVET was shorter than the LVET. The mean RPEP/RVET was 0.53 (range 0.39-0.76) in patients with intact ventricular septum and 0.52 (range 0.54-0.60) in patients with ventricular septal defect (VSD). The RPEP/RVET was significantly greater in patients with D-TGA than in normal controls. The mean LPEP/LVET was 0.27 (range 0.16-0.42) in patients with intact ventricular septum, and 0.34 (range 0.23-0.40) in patients with VSD. The LPEP/LVET was significantly less in patients with intact ventricular septum than in normal controls. The flow velocity patterns of the RVOT and LVOT were the same as those for normal controls. The mean AcT/RVET ratio was 0.51 (range 0.49-0.55) in patients with intact ventricular septum and 0.49 (range 0.39-0.67) in patients with VSD. The mean AcT/LVET was 0.32 (range 0.27-0.38) in patients with intact ventricular septum and 0.28 (range 0.20-0.45) in patients with VSD. The flow patterns did not change after intraatrial baffle repair. Our studies indicate that the flow velocity patterns of the RVOT and LVOT in patients with D-TGA were not reversed, in spite of inverted afterloads.

Echocardiography↗
PubMed1986

[Differences in the immunostimulatory activity and antitumor therapeutic effect of OK-432 and N-CWS].

Immunobiological activities of OK-432 and N-CWS were studied in BALB/c-Meth-A and C3H-MH 134 systems. Peritoneal exudate cells obtained from mice injected i.p. with OK-432 or N-CWS showed stronger in vitro cytotoxicity against Meth-A and YAC-1 cells than did those from non-treated mice. Spleen cells from OK-432 or N-CWS-treated mice exhibited lower Con A mitogenic response in comparison with those from non-treated mice. When Meth-A or MH-134 tumor cells were admixed with OK-432 or N-CWS, the intradermally inoculated tumor cells showed no or slower growth than did those admixed with saline only. There were no significant differences in all the above mentioned activities between OK-432 and N-CWS, and there was no difference in the direct tumor inhibitory activity of the two agents in vitro. However, the administration of N-CWS had no therapeutic effect against i.p.-inoculated tumor cells while OK-432 showed a strong therapeutic effect by the same treatment schedule. Further studies are required to clarify the mechanisms responsible for the difference in the therapeutic effect of the two agents.

Animals↗
PubMed1986

[Auxiliary value of TPA in diagnosing neoplastic diseases].

The auxiliary value of TPA in diagnosing neoplastic diseases was compared to other tumor markers such as CEA, IAP and ferritin. The study population consisted of 59 patients with neoplastic diseases and 75 with benign diseases. The percentages of positive cases for TPA, CEA, IPA and ferritin were 58.9%, 39.0%, 66.7%, and 28.6% in neoplastic diseases and 4.5%, 1.4%, 47.1% and 19.7% in benign diseases, respectively. Both the specificity and sensitivity of TPA were as high as those of CEA. However, the positive rate of TPA was lower than that of CEA in lung cancer patients at stages I and II. TPA was indicated to be a suitable marker for combination assay with CEA in diagnosing neoplastic diseases.

Carcinoembryonic Antigen↗