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Biomedical subjects

S Yalcin

Publications and source records attributed to S Yalcin.

29 records · Page 2Linked to original sources
PubMed1992

Experimental infection of cynomolgus and African green monkeys with human herpesvirus 6.

Cynomolgus and African green monkeys were inoculated with human herpesvirus 6 (HHV-6). An antibody response was first observed 10 days and 5 days after inoculation of cynomolgus monkeys and African green monkeys, respectively, and was detectable for the duration of the experiment (33 days). HHV-6 DNA was first detected by the polymerase chain reaction in mononuclear cells of one cynomolgus monkey and one African green monkey 10 days after virus inoculation, and in a total of three of four cynomolgus monkeys (75%) and four of five African green monkeys (80%) later after inoculation. Furthermore, HHV-6 DNA was detected in the lymph nodes and spleen of monkeys killed 33 days after virus inoculation. A rash was observed on the trunk of one African green monkey 13 days after virus inoculation, otherwise the infection was asymptomatic. When mononuclear cells from both groups of monkeys were cultured in medium containing concanavalin A and interleukin 2, and infected with HHV-6 in vitro, virus replication was observed. The data suggest that HHV-6 infects these species of monkey and that this system could be useful as an animal model of HHV-6 infection.

Animals↗
PubMed1989

Establishment of titration system for human herpesvirus 6 and evaluation of neutralizing antibody response to the virus.

The susceptibilities of seven T-cell lines to human herpesvirus 6 (HHV-6) infection were examined. MT-4 cells were the most susceptible of these lines to infection with this virus. Therefore, chemically adhered MT-4 cell monolayers were used for infectious HHV-6 assay by indirect immunofluorescent-antibody (IFA) staining. When cell monolayers were fixed 30 to 45 h postinfection, the foci stained with IFA were easy to count and a linear relationship was observed between the number of foci and the virus concentration. MT-4 cell monolayers were also used for a focus reduction neutralizing-antibody test. In this test, sera from patients in the convalescent stage of exanthem subitum all showed significant neutralizing activity (1:80 to 1:320), whereas sera from patients in the acute stage of disease showed no detectable neutralizing activity. The titers of neutralizing antibody correlated well with the levels of anti-HHV-6 antibodies detected by IFA.

Antibodies, Viral↗
PubMedDate not supplied

Histologic and histomorphometric comparison of immediately placed hydroxyapatite-coated and titanium plasma-sprayed implants: a pilot study in dogs.

The purpose of this pilot study was to make a histologic and histomorphometric comparison of hydroxyapatite-(HA) coated and titanium plasma-sprayed (TPS) root-form implants that were placed in 2 mongrel dogs immediately after extraction of mandibular premolars. After 8 weeks of healing, the implant-containing segments of the mandible were removed en bloc and bone blocks including implants were sectioned. Histologic and histomorphometric analyses were performed by evaluating bone sections. The mean bone contact percentage of HA-coated implants was 61.84 +/- 7.84%, with a range of 52.09% to 75.7%, and the mean bone contact percentage of TPS implants was 51.35 +/- 12.1%, with a range of 30.1% to 70.6%. This pilot study suggests that HA-coated implants placed into fresh extraction sockets can achieve better bone contact than TPS implants, but there was evidence that the surface of the HA layer can be resorbed, so long-term stability of HA coatings in immediate implantation must be investigated.

Animals↗