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Biomedical subjects

S Wang

Publications and source records attributed to S Wang.

At least 775 records · Page 43Linked to original sources

[The influential factors in using cadmium reduction method for measurement of the stable products of NO--nitrates and nitrites].

To establish an efficient assay method for detecting nitric oxide indirectly, we compared the effects of cadmium filing, 5 mmol/L and 80 mmol/L copperized cadmium filing on the measurement of nitrates and nitrites (the stable products of NO) using the cadmium reduction method. The results demonstrated that cadmium filing was the most efficient cadmium preparations (P < 0.001). It's mean reduction rate was 96%, and it showed stronger reduction effectiveness in deproteinization cell culture media. The results indicated that cadmium filing has strong anti-interference capacity in biological fluid. We recommend the cadmium filing reduction method because it is simple, practical, inexpensive, highly efficient and can be performed in ordinary laboratories.

Aorta↗

[Determination of alkaloids in radix Sophorae flavescentis by high performance capillary electrophoresis].

OBJECTIVE: To develop a simple and rapid method for the determination of alkaloids in Radix Sophorae Flavescentis (RSF). METHOD: HPCE buffer: 20 mmol.L-1 phosphates, pH 7.0; V = 20 kV, leff = 50 cm, L = 64.5 cm, i.d. = 50 microns, lambda = 201 nm, T = 25 degrees C, sample size = 10 kPa.s. RESULT: Matrine(I) and oxymatrine(II) in RSF were completely separated with recoveries of 97.47% and 97.67% respectively. CONCLUSION: Simple, accurate and reproducible, this method can be used for the quality control of Chinese herbal medicines.

Alkaloids↗

[A method of detecting respiratory rate with a high-sensitivity capacitance transducer].

Objective. To introduce a simple and low cost respiratory rate detection method. Method. A high-sensitivity capacitance transducer and corresponding detecting system were used in the system. Result. Satisfactory signals were obtained at the upper portion of the right thorax with the method. Conclusion. It could be used widely. It can be used to comprise the respiratory module in multi-parameter patient monitoring, or be used alone to detect respiratory rate.

Equipment Design↗

[Morphologic characteristics of dental cuticle on teeth affected by diabetic periodontitis].

OBJECTIVE: To determine the nature of the dental cuticle found on teeth with diabetic periodontitis. METHODS: Thirteen permanent teeth which were extracted from three diabetic patients with periodontitis were studied, with the control group of 16 healthy teeth extracted for orthodontical reasons, then all the teeth were decalcified with 5% nitric acid, stained with HE, and examinated by using light microscope. RESULTS: A layer of thick coat, dental cuticle, covered the root surface of the teeth with diabetic periodontitis, and the cuticle which presented regular laminar structure extended from the cementoenamel junction to the apical portion with different thickness from 40 to 80 microns, but no such similar cuticle structure was found on the healthy teeth of the control group. CONCLUSION: There is abnormal cuticle structure on the teeth of diabetic patients with periodontitis.

Aged↗

[Diagnostic value of ultrasonography in cervical lymph node metastasis].

OBJECTIVE: To research the accuracy of ultrasonography in the diagnosis of cervical lymph node metastasis and the value of that of cervical lymph node occult metastasis. METHOD: Fifty-five patients(61 sides) operated by neck dissection were double-blindedly studied by preoperation palpation and ultrasonography comparing with postoperating pathological examination. RESULT: Fifty-two sides were confirmed to be pathology lymph node metastasis from 61 sides. 40 and 48 sides were checked out by palpation and ultrasonography, respectively. The sensitivity, specificity and accuracy of palpation and ultrasonography were 76.9% and 92.3% (P < 0.05), 77.8% and 88.9%(P > 0.05) and 77.0% and 91.8%(P < 0.05), respectively. Six sides were checked out by ultrasonography (occult metastasis) in 12 sides of palpation negative. CONCLUSION: The accuracy of ultrasonography was prior to that of palpation in examining cervical lymph node metastsis. Ultrasonography could check out about half occult metastasis and should be regarded as a routine examining items in diagnosing cervical lymph node metastasis.

Adult↗

[Improvements on radical neck dissection].

OBJECTIVE: To improve the surgery of radical neck dissection for a better curative effect. METHOD: Some improvements on incision, path to exploration and manipulation of operation were made in 65 patients with head and neck tumors while radical neck dissection were done. RESULT: The average surgical time was 110 minutes and the average amount of bleeding was 120 ml in the patients when radical neck dissection had been completed. The complications decreased to 1.54%. The surviual rates of 2 and 3 years are 86.2% (56/65) and 81.6 (31/38) respectively. CONCLUSION: With these improvements on radical neck dissection, the curative effect in the near future is improved.

Adolescent↗

[Design of CXLY-II low frequency magnetic fields pulse generator].

An experimental device, low frequency magnetic fields pulse generator, has been designed and accomplished in our department. It can provide low frequency magnetic fields pulse with the intensity from 150mT to 800mT and the frequency from 0 to 100Hz. This device is easy to operate and performs reliably. It can stably work for a long time and has been successful used in the experiment of magnetic biological effect.

Electromagnetic Phenomena↗

[Blood pressure examination using oscillometric method].

The effect of pressure sensor, filter and blood pressure algorithm upon oscillometric method is discussed in this paper. The design principle and parameters configuration used in portable blood pressure HOLTER are presented.

Algorithms↗

[Studies on the parasites of Paris polyphylla var. yunnanensis].

Two bacteria and three fungi were isolated from the hypogeal stems of Paris polyphylla var. yunnanensis. The bacteria were identified as Bacillus cereus and Pseudomonas alcaligenes, and the fungi were identified as Periconia sp., Pachnocybe albida and Hormomyces paridiphilus. The results on liquid culture of B. cereus, P. alcaligenes and H. paridiphilus indicated that the colloidization and polysaccharide content increasing in hypogeal stems of P. polyphylla var. yunnanensis were due to the extracellular polysaccharide secretion of the parasitical fungus H. paridiphilus.

Bacillus cereus↗

[Detection of Plasmodium vivax by polymerase chain reaction in Hainan].

AIM: To establish a modified polymerase chain reaction(PCR) method for the detection of Plasmodium vivax in the endemic areas of malaria and compare the method with the conventional light microscopy in the field. METHODS: A PCR method was modified by improving the collection procedures of blood samples, template extraction, primer design and optimizing the reaction condition. The method was evaluated by examining blood samples from 310 patients with vivax malaria and compared with the conventional light microscopy in endemic areas of Hainan Province. RESULTS: The positive rates of the modified PCR method and microscopic method were 34.2% and 31.9%, respectively. CONCLUSION: The modified PCR method is simple, sensitive and specific for the detection of vivax malaria patients in endemic areas.

Adolescent↗

[Studies on recombinant chitinase and SXP-1 antigens as antimicrofilarial vaccines].

AIM: To determine whether immunization with recombinant filarial chitinase or a fragment containing the epitope recognized by McAbMF1 and SXP-1 could protect jirds against microfilaremia resulting from infection with B. malayi. METHODS: Test jirds were immunized with the following recombinant parasite antigens: filarial chitinase, the c-terminal fragments F7R2 or F8R2 of r-chitinase, filarial SXP-1, myosin or maltose binding protein (MBP). Employing immunochemical techniqe (SDS-PAGE, Western Blotting) and serology (ELISA) measured antifilarial antibodies level. RESULTS: Immunization of jirds with recombinant chitinase induced partial protection against microfilaremia resulting from subsequent infection with B. malayi, but did not reduce adult worm burdens. Vaccination was much less effective when administered during the prepatent stage of infection and was ineffective when given to microfilaremic jirds. Immunization of jirds with SXP-1, an antigen present in multiple worm stages also reduced microfilaremia and, in some experiments, adult worm burdens. CONCLUSION: The recombinant chitinase, fragments F7R2 and F8R2 and SXP-1 could provide partial protection against microfilaremia in jirds.

Animals↗

[A pilot study on malaria control by using a new strategy of combining strengthening infection source treatment and health education in mountainous areas of Hainan province].

AIM: To explore a new malaria control strategy that fits current epiodemiological condition and coincides with modern medicine model and the principle of cost-benefit. METHODS: The new strategy highlights the risk villages and risk population as the focal point and integrates health education with behavioral intervention. The main anti-malaria measures consists of carrying out health education in risk villages, giving mass drug administrations in risk population staying overnight in the mountain, following up malaria cases for implementing radical cure, but without using traditional residual spraying or impregnating bednets with insecticides. RESULTS: After having adopted the new strategy and taken the control measures, the people's knowledge about malaria increased to a higher level and the indices of malaria reduced to a lower level. The rate of bednet-using in the population was increased from 26.8% to 72.6%. The annual parasite incidence (API) of malaria was declined from 3.5% in 1994 to 1.1% in 1996 and 0.8% in 1997, and the API of falciparum malaria was declined from 1.0% to 0.3% and 0.3% respectively in the townships at the same time. The parasite rate(PR) of malaria was declined from 7.2% in May, 1995 to 2.1% in November, 1996 and 1.2% in October, 1997 and the PR of falciparum malaria was declined from 1.2% in May, 1995 to 0.1% in October, 1997. The proportion of villages without malaria cases was increased from 18.6% in 1994 to 54.2% in 1997, and the number of risk villages with a malaria incidence above 5% was reduced from 14 to 2 at the same time. The ratio of cost/benefit was 1:2.4 in 1995-1996 and 1:4.4 in 1997, showing a better economic benefit. CONCLUSION: The expectant result has been obtained, thereby providing new experience for the malaria control in the mountainous areas of Hainan Province.

China↗

[Structural disorder and conformation difference in the organic molecular crystals].

OBJECTIVE: Investigating multiple configuration nature of natural organic molecules through study on atom disorder in crystal state molecules. METHODS: Three-dimensional determination for four natural organic compound samples by using single crystal X-ray diffraction. RESULTS: Through disorder analysis on molecules in crystal state, we have found that the disorder of some atoms may cause conformation difference for molecules, bring about multiple conformation in crystal state hence reducing the symmetry of molecules in crystal cells. CONCLUSIONS: There can be two or three molecules, even two different compounds in an asymmetrical unit of structure cell because of partial disorder or configuration difference in organic molecule crystals.

Crystallography↗

[Experimental studies of Chinese taihe chicken on effects of "Qi" reinforcing and "Yin" nourishing].

The "Qi" deficiency and "Yin" deficiency rats were fed with Chinese Taihe Chicken or common Chicken. The results showed: Chinese Taihe Chicken and common Chicken could all improve in part the "Qi" deficiency of rats caused by forced swimming, furthermore, the effect of Chinese Taihe Chicken was better than that of common Chicken. Chinese Taihe Chicken could obviously reduce the activity of adenosine triphosphatase of kidney tissue of "Yin" deficiency rats caused by injecting thyroxine. It suggested that Chinese Taihe Chicken could nourish the "Yin".

Adenosine Triphosphate↗

[The pathogenicity of HFRS patient's soft palate mucosa to suckling mice].

OBJECTIVE: To explore the pathogenic mechanism of hemorrhagic fever with renal syndrome(HFRS). METHODS: 2-3 days old inbred Balb/C suckling mice were inoculated intracerebrally with tissue suspension of soft palate petechia of HFRS patients and the HFRS virus RNA was tested in brain, lung and kidney tissues of sucking mice by nested PCR. RESULTS: Some of the mice developed acute disease and died. The autopsy analysis indicated that these mice showed pathological changes in microvascular system and parenchymatous tissues. HFRS virus RNA was detected in their brains, lungs and kidney tissues. CONCLUSIONS: These results indicate that HFRS virus exists in the tissue of petechial hemorrhagic spots on soft palate petechia of HFRS patients.

Animals↗

CD28/B7-mediated costimulation is required for parathyroid gland allograft rejection in rats.

The activation of T cells to differentiate and to proliferate is an essential step in the immune response to antigen, especially in cell mediated acute allograft rejection. Besides the interaction of CD3/TCR complex with Ag/MHC complex presented on antigen-presenting cells, a complete T-cell activation and proliferation requires a second costimulatory signal. The interaction of CD28/CTLA-4 and B7 is a major costimulatory pathway for T Cell activation. Inhibition of this pathway results in development of antigen-specific unresponsiveness and clonal anergy. In present study, the biologic function of anti-CD28 monoclonal antibody and its Fab fragment were investigated in vitro and in vivo. The results indicate that mAbCD28 and its Fab fragments could promote the functional recovery of allografts and prolong the graft survival, but could not reverse the acute rejection or induce transplantation tolerance in the rat PTG allograft model. We also found that peripheral TNF-alpha level and NK-cell activity were suppressed in the presence of mAbCD28 and its Fab fragments for a relatively long time after PTG transplantation.

Animals↗