Biomedical subjects

S Wang

Publications and source records attributed to S Wang.

At least 559 records · Page 31Linked to original sources

PubMed2000

[Intraperitoneal lymphatic chemotherapy for colonic cancer].

OBJECTIVES: To evaluate the effect of intraperitoneal lymphatic chemotherapy with carboplatin and sandostatin on colonic carcinoma and abdominal lymphatic metastasis in patients after radical resection. METHODS: Patients with colonic cancer were divided into two group: abdominal lymphatic chemotherapy group and control group. The lymphatic chemotherapy group was treated at the introduction stage of anesthesia with carboplatin (200 mg) injected intraperitoneally and sandostatin (0.1 mg x 2) injected subcutaneously, whereas the control group was just treated with carboplatin (200 mg) injected intraperitoneally. The platinum levels in abdominal lymphatic nodes were monitored during operation. The two groups were uninterruptedly given intraperitoneal lymphatic chemotherapy or intraperitoneal chemotherapy. Retroperitoneal lymphatic metastasis was evaluated in each patient by abdominal CT scan after operation. RESULTS: The platinum levels in abdominal lymphatic nodes increased more significantly at 2, 3 hours in the lymphatic chemotherapy group than in the control group (P < 0.01). There was no difference in the platinum levels of the portal vein between the two groups, and also no difference in the platinum levels between the lymphatic nodes with or without metastasis. The rates of retroperitoneal lymphatic metastasis after resection were significantly lower in the lymphatic chemotherapy group control group (P < 0.01). CONCLUSION: Intraperitoneal administration of carboplatin combined with sandostatin may elevate the platinum levels in the abdominal lymphatic nodes and reduce the abdominal lymphatic metastasis after radical resection of colonic cancer. It is an effective method for intraperitoneal lymphatic chemotherapy.

Antineoplastic Combined Chemotherapy Protocols↗

PubMed2000

[Relationship between estrogen receptor and Graves' disease].

OBJECTIVE: To study the relation of expression of estrogen receptor (ER) in thyroid tissues with Graves' disease (GD). METHODS: ER was detected in the tissues of 40 patients with GD and 15 patients with normal thyroid by immunohistochemical method. Gene expression of ER was studied in frozen slides of 26 GD thyroids and 10 normal thyroids by fluorescence in situ hybridization (FISH) together with image analysis system. RESULTS: In immunohistochemical detection, the positive rate of ER in GD group was 70% (28/40), but 13.3% (2/15) in the control group (P < 0.01). The positive degree of ER in female with GD was higher than that in male with GD (P < 0.05). The positive rate of ER in the female GD group below 35 years was 85.0% (17/20) and 50% (5/10) in the female GD group above 35 years (P < 0.05). In FISH, the optical density (A) value of the female GD group was 30.14 +/- 10.74, and in the male GD group 21.06 +/- 7.71, but in the control group it was 16.91 +/- 5.30 (P < 0.01). The A value of the female GD group below 35 years was 33.85 +/- 10.10, and 23.24 +/- 7.48 in the female GD group above 35 years (P < 0.05). CONCLUSIONS: The increasing of ER content is related to the development of GD. Estrogen may be a promoting factor for GD. The sex and age differences in GD development are associated with the effects of estrogen and ER.

Adult↗

PubMed2000

[Clinical and pathological characteristics of gastric stump cancer and recurrent gastric stump cancer].

OBJECTIVE: To study early diagnosis and rational treatment of gastric stump cancer and recurrent gastric stump cancer. METHODS: The data of 27 patients with gastric stump cancer and 23 patients with recurrent gastric stump cancer were analysed retrospectively during the past 20 years based on their clinical and pathological findings. RESULTS: The onset time was 10 years or more for gastric stump cancer and less than 10 years for recurrent gastric stump cancer after gastrectomy. Gastroscopy was better than double contrast radiography in diagnosis. Partial resection of gastric stump was performed for patients with early cancer at the anastomotic stoma of gastrojejunostomy. Total resection of gastric stump was made for other patients. Lymph node of the mesojejunum were dissected routinely because their metastasis rate was about 40.0% in patients with cancer of anastomotic stoma. The effect of surgical treatment was poor for patients with advanced cancer. CONCLUSIONS: Regular gastroscopy is useful in detecting gastric stump cancer in patients with gastric cancer after gastrectomy, or in those years after removal benign. Malignancy was more evident in recurrent gastric stump cancer than in primary gastric cancer. Prognosis is good for early stage cases and poor for late stage cases after surgical treatment.

Adult↗

PubMed2000

[An observation of the immuno-persistence after inoculating with the domestic BRD II strain rubella vaccine among infants and young children].

OBJECTIVE: To study the immuno-persistence after inoculating with the domestic BRD II strain rubella vaccine among infants and young children. METHODS: Hemagglutination inhibition (HI) antibody detection method was used to test on children at age 6 to 18 months without rubella infection or rubella vaccine immunization in Yantai city of Shandong province and were selected for the observation of the immuno-persistence against the domestic BRD II strain rubella vaccine from 1995 to 1998. RESULTS: Positive rates of HI antibody among children of 6, 7, 8, 9, 12 and 18 month-old of inoculation were 94.44%, 97.22%, 96.67%, 100.00%, 100.00%, 100.00% and 93.10%, 93.55%, 96.77%, 96.97%, 100.00%, 100.00% (P > 0.05), in one or two years respectively. The geometric mean reverse titers (GMRTs) were about 50% lower than that after 1 month after 1 year. Similar results were found that after 2 years GMRTs was about 50% lower than that after 1 year of inoculation. There was highly significant difference on GMRTs of HI antibody between infants with 6 to 7 month-olds (29.89) and young children with 8 to 18 month-olds (53.00) after 2 years of inoculation (t = 3.58, P < 0.001). CONCLUSION: The immunization schedule at the first dose for BRD II strain rubella vaccine should be started when the child is 8 month old. For the second dose, the immunization schedule used in other developed countries should be referred adopted in China.

Antibodies, Viral↗

PubMed2000

[Community -based comprehensive prevention and control of hypertension in China (CCPACH Study)-prevalence and epidemiological characteristics in urban and rural area].

OBJECTIVE: To understand the epidemiological features and prevalence of essential hypertension in the rural communities. METHODS: A community-based survey on essential hypertension was carried out in urban and rural areas involving 280,000 population. RESULTS: The prevalence rate of hypertension in age 35 years and over was 31.7% and 32.9% in urban and rural respectively; the average age of the patients' in urbans was significantly higher than in rural areas. Among non-hypertensive cases, more than 50% of the population having risk factors of hypertension in both urban and rural areas. Rates of treatment and control awareness of hypertension were 56.3%, 26.8% and 4.4% respectively in urban; comparing to 40.3%, 17.5% and 2.6% respectively, in the rural areas. More than half of the diagnosed patients before survey did not take antihypertensive medicine for treatment in both areas. The proportions the classification of hypertension were similar in both areas with majority of them, mild and isolated systolic hypertension. CONCLUSIONS: It was demonstrated that there is an urgent need to carry out comprehensive prevention and control for hypertension in urban and rural area.

Adult↗

PubMed2000

[Evaluation on the effects of community-based comprehensive prevention and control of hypertension in the rural areas in China].

OBJECTIVE: To explore the strategies and measures of community-based comprehensive prevention and control of hypertension in the rural areas. METHODS: Community-based comprehensive prevention and control of hypertension was put in practice in 120,000 people in Fangshan District of Beijing during the period of "The Ninth Five-Year Plan (1996 - 2000)". RESULTS: After intervention, blood pressure of the whole subjects were under control and kept the level of baseline data; the rate of increase of prevalence in the intervention areas was below the rate of that from 1979 to 1991 in China (the former was 14.7%, while the latter was 53.7%); the incidence rate of the subjects was 1.4%, close to the average rate of age 15 and above in China; the rates of awareness, treatment and control were above the levels of baseline data; SBP and DBP of the patients decreased by 2.9% and 5.6%, respectively; while the rate of having high risk factors reduced by 13.4% on average. CONCLUSION: The community-based comprehensive strategies and measures played an important and effective role in prevention and control of hypertension in rural areas.

Adult↗

PubMed2000

[The recognition of the epidemic area of tsutsugamushi disease on Nan Peng Lie Island in China and the strategy of prevention].

OBJECTIVE: To investigate the region for the epidemic area of tsutsugamushi disease. METHODS: Epidemiological studies, Orientia tsutsugamushi isolation, and preventive measures were used. RESULTS: The region belonged to epidemic area of south subtropical zone. The main host was found Rattus norvegicu. The main biological vector was Leptotrombidium deliens. The seasonal trends of the quantity of Rattus norvegicu and Leptotrombidium deliens were consistent with the incidence change of the disease in the region. Orientia tsutsugamushi has been isolated from Rattus norvegicu and Leptotrombidium deliens. Data showed that Orientia tsutsugamushi isolated strains mainly belongs to Karp. Sero-epidemiological data showed that antibodies of Orientia tsutsugamushi are 100.0% positive in the residents of the region and 4.0% in army men. After taking comprehensive preventive measures, the incidence was descending. CONCLUSION: Nan Peng Lie island was proved an epidemic area of tsutsugamushi disease.

Animals↗

PubMed2000

[An epidemiological analysis on the relationship between road injury and traffic environment in China].

OBJECTIVE: To explore the influence of traffic environment on road injury so as to provide basis for prevention and control. METHODS: Principal component regression analysis was used to explain the relationship between road injury and the numbers of vehicle, capacity of road transport and mileage of rigid highway. RESULTS: Results showed a positive correlation between level of personal safety (LPS) and the numbers of vehicle, volume of road haulage, volume of passenger transport, mileage of rigid highway (correlation coefficients were 0.8714, 0.9691, 0.9611, 0.9510, P = 0.0005). The numbers of vehicle, volume of road haulage, volume of passenger transport and mileage of rigid highway were increasing when LPS decreased. Principal component regression analysis overcame multi collinearty of independent variables and obtained a regression equation y =-3.7197 + 1.49E-03X(1) + 5.2E-06X(3) + 6.19E-02X(4). CONCLUSION: The primary determinants of LPS were numbers of vehicle, volume of road haulage, volume of passenger and mileage of rigid highway. Road injury thus could be reduced through improving the traffic environment, strengthening the traffic administration as well as promoting traffic safety.

Accidents, Traffic↗

PubMed2000

[A study on relationship between plasma heat stress protein 70 and cytokine in patients with heat apoplexy].

OBJECTIVE: To study the change of levels of plasma heat stress protein 70 (HSP70) and cytokine and their interrelationship in pathogenicity of heat apoplexy. METHODS: Plasma HSP70 was determined with Western blot, and plasma interleukin-2 (IL-2), soluble interleukin-2 receptor (sIL-2R) and interleukin-6 (IL-6) were determined with enzyme-linked immunosorbent assay (ELISA). RESULTS: Plasma level of HSP70 was 4 211.2 +/- 1,286.2 (integral optical density) in average in the patients of heat apoplexy group, 4,137.8 +/- 1, 207.5 in those of severe heat apoplexy group, and 6,043.5 +/- 1,354.8 in control group, with very significant difference (P < 0.01). Plasma IL-2 level was (64.3 +/- 32.3) pg/ml and (200.0 +/- 50.0) pg/ml and sIL-2R (54.9 +/- 33.3) U/ml and (167.5 +/- 89.0) U/ml in the patients with heat apoplexy and in controls, respectively, with very significant difference. But, there was no significant difference in IL-6 between varied groups. CONCLUSIONS: The above-mentioned results suggest that establishment of heat acclimatization correlates to the interaction of network of neuroendocrine-cytokine-HSP, in which decrease in plasma IL-2 was one of the important factors causing reduction of HSP70 expression. Balance of network regulation was broken by pathological factors, finally resulting in heat apoplexy.

Adolescent↗

PubMed2000

[Detection of C gene mutation strain in vertical transmission of hepatitis B virus and its significance].

OBJECTIVE: This study aims at detecting C gene mutation strain in vertical transmission of hepatitis B virus (HBV). METHODS: HBV C gene nt 2,022 - 2,321 was directly sequenced. RESULTS: Homology of HBV nucleotide sequence and amino acid sequence in mothers, fathers and their fetus was 100%. Mutation of 2,189 and 2,288 nucleotide of C gene resulted in substitution at the 97th and 130th amino acids. CONCLUSION: C gene mutation strain is present in vertical transmission of HBV. Presence of mutation strain may be the cause of failure in vaccination.

Adult↗

PubMed2000

[A study on incidence of injury and its socio-economic loss in children and young adults].

OBJECTIVES: To study the current status of incidence of injury among children and young adults and the causes of common injuries, and to estimate its socio-economic loss and extent of harmfulness. METHODS: Pupils in 19 primary and middle schools aged 7 to 18 years, totaling 14,533, were recruited with stratified cluster sampling during 1998 to 1999 in Guangzhou, Maoming, Jiangmen and Shantou. Judgement for injury was based on the following criteria: (1) diagnosed and treated in hospitals or school clinics, (2) a half-day off or more due to injury, and (3) emergency management by pupils' parents or teachers. RESULTS: There were 6 941 pupils suffered from varied injures during the year, with an incidence rate of 47.76% (50.08% for boys and 45.02% for girls). Incidence rate of injury was higher in the middle school pupils (13 - 18 years old, 58.49%) than that in the primary school pupils (7 - 12 years old, 40.08%). The incidence increased significantly with age, with a peak at ages of 13 - 15. Major causes of injuries resulted from falls, injury by sharp articles, collision, traffic injuries and burn/scalds, etc, which usually occurred due to carelessness in sports, playing, walking, bike-riding and working. Frequency of multiple injuries related to the educational level of parents and depended on whether or not an only-child in family. There were 2,173 injured pupils (accounting for 31.3% of the total) visited clinics or emergency department in hospitals and 627 (9.0%) hospitalized for treatment. Twenty-eight percent of the injured pupils were absent from school, with an average absenteeism of 5.6 days. There were 154 injured pupils with transient disability and 53 with permanent disability, with a disability rate of 410.47 per 100,000. Cost for their medical care averaged 81.5 yuan RMB per injured pupil. CONCLUSIONS: Injury was a common and frequently-happened incident among children and young adults and could seriously affect their health, development and growth, studying and their future of children and young adults. Health education, safety-promotion and removing hidden danger for injury are the major measures to prevent from injury, and the government and the whole society should pay great attention to injury issues in children and young adults.

Accidents↗

PubMed2000

[Expression and clinical significance of p53 protein and p21(WAF1) in non-small-cell lung carcinoma].

OBJECTIVE: To investigate the relationships between expression of tumor suppressor gene p53 protein, p21(WAF1), clinicopathology and prognosis in human non-small-cell lung carcinoma (NSCLC). METHODS: SP immunohistochemistry performed in all cases. RESULTS: One hundred forty-seven resected NSCLC were examined. There were 63 squamous cell carcinomas, 66 adenocarcinomas, 14 adenosquamous carcinomas and 4 large cell carcinomas. Overall 90 (61.2%) of the 147 cases had positive p53 staining. The positive ratios of p53 overexpression of squamous cell carcinomas, adenocarcinomas, adenosquamous carcinomas and large cell carcinomas were 63.5% (40/63), 57.6% (38/66), 71.4% (10/14) and 50% (2/4) respectively. The average positive ratio of p21(WAF1) expression was 40.1% (59/147) in lung carcinoma. The positive ratios of p21(WAF1) expression of squamous cell carcinomas, adenocarcinomas, adenosquamous carcinomas and large cell carcinomas were 41.3% (26/63), 42.4% (28/66), 28.6% (4/14) and 25% (1/4) respectively. Over expression of p53 protein was associated with prognosis of the patients with adenocarcinomas. The positive ratio of p53 expression in over 3 year survival patients was 75% (21/28) and in less than 3 year survival, 44.7% (17/38) (P < 0.025). The positive expression of p21(WAF1) was related to prognosis of NSCLC. The ratio of 3-year survival (64.4%) in p21(WAF1) positive NSCLC was much higher than in p21(WAF1) negative patients (46.6%) (P < 0.05). Prognosis of patients with p53 positive but p21(WAF1) negative expression was poorer than those with p53 negative and p21(WAF1) positive NSCLC (P < 0.01). CONCLUSION: These findings suggest that over expression of p53 in adenocarcinomas could be used as a prognostic factor for patients. p21(WAF1) is of protein has a definite value in judging the prognosis in NSCLC. Combined expression of p53 and p21(WAF1) can be used as the prognostic markers in NSCLC and are of significance in estimating the prognosis of patients.

Adenocarcinoma↗

PubMed2000

[Aortic calcification and matrix metalloproteinases].

OBJECTIVE: To study the relationship between arterial calcification and matrix metalloproteinases including MMP-1 and MMP-3. METHODS: 15 human aorta specimens from autopsies were stained with HE to identify calcification. Immunohistochemical method was used to determine the secretion of MMP-1 and MMP-3. RESULTS: Among the 15 aorta specimens, calcification was found in 10 and no calcification in the remaining 5. There was no significant difference in the percentage of MMP-1 positive cells between two groups, with 83.8% +/- 5.2% in calcified specimens vs 84.0% +/- 7.5% in non calcified ones. 9 of the 10 calcified aortas were positive for MMP-3 staining. Serial sections showed that the localization of positivity was in concordance with calcified regions. The 5 normal aortas were MMP-3 negative. The difference between the two groups was significant. CONCLUSION: MMP-3 secretion relates closely with arterial calcification. And MMP-3 may participate in calcium minerali-zation in arteries. MMP-1 has no relation with arterial calcification.

Aorta↗

PubMed2000

[The role of PMNs in early progressive injury of deep partial-thickness burn wound].

OBJECTIVE: To examine the role of polymorphonuclear neutrophils (PMNs) in early progressive injury of deep partial-thickness burn wound. METHODS: After deep partial-thickness burn, the count of PMNs in beripheral circulation, activity of myeloperoxidase (MPO) and content of MDA in wound tissue of Spraque Dawley rats, were measured. Sections of wound biopsies were stained with Masson's thrichromatic staining and anti-vimentin immunohistopathologic staining for identifying the injured tissue. RESULTS: PMNs counts increased immediately and reached a peak at 4 h postburn, and MPO activity peaked at 24 h postburn. MDA content was lowe at 0.5 h and higher at 48 h postburn than that of normal control. Histologic analysis showed that there was more necrotic tissue at 48 hr postburn than that at 24 h. CONCLUSION: It was after 24 h postburn that PMNs induced tissue damage. The results suggest that intervention of PMNs adhesion should be under taken in about 4 h after thermal injury and anti-oxidizing agent should be given before 24 h postburn in order to prevent early progressive injury to the wound.

Animals↗

PubMed2000

[An experimental study of antibiotic -- induced endotoxin release in Pseudomonas aeruginosa bacteremia inburned rats].

OBJECTIVE: To assess the intensity of endotoxin release in Pseudomonas aeruginosa challenged burned rats after the administration of various antibiotics. METHODS: Rats were inflicted with 30% TBSA burn, and they were challenged with Pseudomonas aeruginosa (PA103). After the administration of different beta lactam antibiotics, serum endotoxin level and blood bacterial count were determined. RESULTS: All the antibiotics used were effective in eradicating the bacteria with concomitant release of endotoxin in varying amounts. It was found that Imipenem released the least amount of endotoxin, followed in order by Cefoperazone, Ceftazidime, and Cefotxime. CONCLUSIONS: Different antibiotics possessed varying capacity of inducing endotoxin release from PA, and the amount released did not bear any relation with the bactericidal capability of the antibiotics.

Animals↗

PubMed2000

[Effects of early enteral feeding and L-arginine supplementation on liver albumin synthesis in burned rats].

OBJECTIVE: To evaluate the effect of early enteral feeding and L-arginine supplementation on mRNA expression of albumin, and some cytokines. METHODS: Twenty Wistar rats prepared with gastrostomy 7 days before the experiment were inflicted with 30% TBSA flame and were divided into early enteral feeding group (EF) and L-arginine enriched early enteral feeding group (AEF). All rats in two group starts enteral feeding two hours after burn. Capillary electrophoresis and reverse transcription-polymerase chain reaction (RT-PCR) were used to measure the mRNA of albumin, TNFa, IL-1a, IL-6R and iNOS respectively on 1st, 3rd, 6th and 9th day post burn (PBD). Serum albumin was observed at the same time. RESULTS: The mRNA expression of albumin on PBD1 was reduced in both group and improved on PBD3, 6, 9. The gene expression of albumin was significantly higher in AEF group than in EF group onPBD3, 9 [(1812 +/- 41) vs (1417 plus minus 43), P < 0.01]. Similarly, serum albumin were very low in two groups on PBD1 and improved on PBD3, 6, 9. Serum albumin in AEF group was significantly higher than in EF group on PBD9 [(31.9 plus minus 2.1) g/L vs (28.1 plus minus 1.3) g/L, P < 0.01]. The mRNA of TNFalpha, IL-1alpha, IL-6R and iNOS were increased on PBD1 and reduced on PBD3, 6, 9. The gene expression of TNFalpha, IL-1alpha in AEF group were significantly lower than EF group on PBD6, while IL-6R differed significantly between AEF group and EF group [(972 +/- 87) vs (2570 +/- 90), P < 0.01] on PBD9. There were no significant difference in iNOS mRNA production after burn in two group. CONCLUSION: Early enteral feeding could improve albumin biosynthesis and reduce the gene expression of TNFalpha, IL-1alpha, IL-6R and iNOS. L-arginine-supplemented early enteral feeding could further increase the gene expression of albumin possibly by reducing the expression of TNFalpha, IL-1alpha and IL-6R and did not increase the expression of iNOS.

Albumins↗

PubMed2000

[Effects of different nutritional support routes on the intestinal mucosa mucosainjury and renovation in burned rats].

OBJECTIVE: To compare the effects of different nutritional support routes on the intestinal mucosa in burned rats. METHODS: Wistar rats inflicted with 30% TBSA full thickness burns were employed as the model and randomly divided into three groups: burn with enteral nutrition (EN), burn with parenteral nutrition (PN), and normal control (C). Solutions for intravenous and oral application with same volume, calorie, nitrogen and other nutrients were administered to rats in PN and EN groups, respectively. The plasma diamine oxidase (DAO) activity, the intestinal mucosa change of proliferating cell nuclear antigen (PCNA), of transmembrane electric potential difference (PD), of intestinal trefoil factor and their correlation analysis (ITF) were observed. RESULTS: Plasma DAO activity increased obviously and PD, PCNA values, ITF content were much lower in EN, PN groups than those in C group. The ITF content, the values of PD and PCNA in EN were much higher than those in PN group, while DAO activity in EN group was obviously lower than that in PN group. In addition, ITF content was significantly negatively correlated to plasma DAO activity, and obviously positively correlated to PCNA and PD values. It is suggested that intestinal mucosa injury was much more severe in PN group than that in EN group. CONCLUSION: Postburn intestine mucosa mucosal structural injury was related to the evident decrease of synthesis and secretion of ITF. Enteral nutrition might slow down the decrement of ITF, which could explain why EN was superior to PN in terms of decreasing intestinal mucosal injury and enhancing intestinal renovation.

Amine Oxidase (Copper-Containing)↗

PubMed2000

[Histochemical study on the nitric oxide synthase in the intestinal myenteric plexus of burned rats].

OBJECTIVE: To elucidate the postburn change in the activity and the distribution of nitric oxide synthase (NOS) in rat intestinal myenteric plexus. METHODS: The activity and distribution of NOS were quantitatively and locationally studied by histochemical staining with NADPH-diaphorase (NDP), a marker for NOS, and whole mount preparation technique. RESULTS: NOS was widely distributed in whole intestine wall, especially in myenteric plexus. The majority of NOS positive neurons displayed either round or oval shape. There were some varicosities string of bead-like structure in the nerve fibers and which were accompanied by blood vessels and muscular fibers. There was no significant postburn change in the density of NOS positive neurons in myenteric plexus (P < 0.05). Nevertheless the NOS activity of neurons decreased obviously (P < 0.05 similar 0.01). Meanwhile, it was found after injury that there were indistinction of the structure of NOS positive neuron, more breaking of nerve fibers, leading to the lack of integral web of nerve fibers and obvious decrease of NOS positive varicosities in nerve fibers. CONCLUSION: After burn injury, the NOS positive neural structure in myenteric plexus of rat small intestine was damaged with the decrease in NOS activity. Simultaneously, there was impairment of the releasing route of nitric oxide (NO). All these changes might be closely related to intestinal structural damage and dysfunction after burn injury.

Animals↗