Search PubMed⌕ Search

Biomedical subjects

S V Rao

Publications and source records attributed to S V Rao.

At least 19 recordsLinked to original sources
PubMed2000

Laying performance of broiler breeder chickens fed various millets or broken rice as a source of energy at a constant nutrient intake.

Laying performance, egg quality and carcase traits were recorded in broiler breeders (29-48 weeks of age) fed diets in which pearl millet (Pennisetum typhoides), finger millet (Eleusine coracana), foxtail millet (Setaria italica) or broken rice fully replaced yellow maize (50% of the diet) from the reference diet. Constant ratios of metabolizable energy to other nutrients were maintained in all the diets. Each diet provided 1.38 MJ metabolizable energy (ME)/bird per day to 8 replicate groups of 10 layers each, maintained in deep litter pens. The hen-day egg production and the ME required to produce 12 eggs in birds fed pearl millet or broken rice were similar (p > 0.05) to those fed on the maize diet, while birds fed foxtail millet or finger millet required more energy to produce the same number of eggs. The egg production and the efficiency of energy utilization were significantly (p < 0.05) reduced in the birds fed these two cereals. Egg quality was not affected by replacing maize with the different cereals. However, the yolk colour index was significantly (p < 0.05) reduced in the cereal-fed groups in comparison to the maize-fed birds. The gizzard and giblet weights were heavier (p < 0.05) in the birds fed foxtail millet or finger millet than in those fed broken rice. Deposition of abdominal fat and the liver fat content were significantly (p < 0.05) higher in the birds fed pearl millet than in the other groups. Depending on the local prices, the cost of feeding may be reduced considerably by using any of the cereals tested as the principal energy source in place of maize in broiler breeder diets.

Animal Feed↗
PubMed1999

Controlled layer-by-layer immobilization of horseradish peroxidase.

Horseradish peroxidase (HRP) was biotinylated with biotinamidocaproate N-hydroxysuccinimide ester (BcapNHS) in a controlled manner to obtain biotinylated horseradish peroxidase (Bcap-HRP) with two biotin moieties per enzyme molecule. Avidin-mediated immobilization of HRP was achieved by first coupling avidin on carboxy-derivatized polystyrene beads using a carbodiimide, followed by the attachment of the disubstituted biotinylated horseradish peroxidase from one of the two biotin moieties through the avidin-biotin interaction (controlled immobilization). Another layer of avidin can be attached to the second biotin on Bcap-HRP, which can serve as a protein linker with additional Bcap-HRP, leading to a layer-by-layer protein assembly of the enzyme. Horseradish peroxidase was also immobilized directly on carboxy-derivatized polystyrene beads by carbodiimide chemistry (conventional method). The reaction kinetics of the native horseradish peroxidase, immobilized horseradish peroxidase (conventional method), controlled immobilized biotinylated horseradish peroxidase on avidin-coated beads, and biotinylated horseradish peroxidase crosslinked to avidin-coated polystyrene beads were all compared. It was observed that in solution the biotinylated horseradish peroxidase retained 81% of the unconjugated enzyme's activity. Also, in solution, horseradish peroxidase and Bcap-HRP were inhibited by high concentrations of the substrate hydrogen peroxide. The controlled immobilized horseradish peroxidase could tolerate much higher concentrations of hydrogen peroxide and, thus, it demonstrates reduced substrate inhibition. Because of this, the activity of controlled immobilized horseradish peroxidase was higher than the activity of Bcap-HRP in solution. It is shown that a layer-by-layer assembly of the immobilized enzyme yields HRP of higher activity per unit surface area of the immobilization support compared to conventionally immobilized enzyme.

Avidin↗
PubMed1999

Immune competence, resistance to Escherichia coli and growth in male broiler parent chicks fed different levels of crude protein.

Immune competence, resistance to Escherichia coli and growth were measured in female chicks of broiler male parent lines from four different commercial sources. These chicks were fed with three levels of dietary crude protein (CP) from day-old. The protein contents in the diets were 18%, 20.5% and 23%; these diets are referred to as the low-, medium- and high-protein diets, respectively. There was a significant genotype by dietary protein interaction for body weight at 35 days of age but not at 14 or 28 days of age. At 14 days of age, the chicks fed on the high-protein diet weighed significantly more than those fed on the low-protein diet, but there were no differences between the chicks fed on the medium-and low-protein diets. The influence of CP content on body weight had disappeared by 28 days of age. There were significant differences between the genotypes-in antibody production in response to sheep red blood cell (SRBC) inoculation, but no such differences were observed between the chicks fed the different levels of dietary protein. Chicks fed on the high-protein diet had lower lesion scores following E. coli inoculation than those fed on the low-protein diet. There were also significant differences in lesion scores among the genotypes. Genotypes with heavier body weights had significantly higher lesion scores and lower antibody titres than those with less body weight. Also, genotypes of lower body weight had a greater cutaneous basophilic hypersensitivity response to phytohaemaglutinin-P inoculation, and a better humoral response against SRBC and a lower heterophil to lymphocyte ratio.

Animals↗
PubMed1997

Glutathione metabolism in uremic rat.

The impact of guanidine hydrochloride, a uremic toxin, has been investigated on glutathione mediated antioxidant defense mechanisms in rat liver and kidney. Elevated glutathione-S-transferase (GST) activity in the tissue of guanidine treated rat indicates its active participation in the detoxification of uremic toxin involving glutathione. Glutathione (GSH) is replenished by elevated glutathione reductase and peroxides formed are subsequently detoxified by augumented selenium and non-selenium dependent glutathione peroxidase activities.

Animals↗
PubMed1997

Glycemic response to malted, popped and roller dried wheat-legume based foods in normal subjects.

A blend consisting of 75% malted and debranned wheat flour, toasted flours of 10% green gram, 5% Bengal gram, 5% moth bean and 5% skim milk powder, consumed by seven healthy adult subjects in the form of 'chapati' (unleavened bread), showed higher blood glucose response after 30 min, as compared with blends of similar composition prepared by adopting popping or roller drying technique. No significant differences were observed in blood glucose response between the three foods at any other time intervals up to 120 min. However, significantly slower response to the popped product was observed in peak blood glucose, area under the curve (AUC) and slope from 0 time to maximal increase in glycemia. All the three foods were digested fast, as blood glucose reached fasting levels after 120 min.

Adult↗
PubMed1997

Inhibitory kinetic studies on rat hepatic and renal succinate dehydrogenase with guanidine.

Guanidine-induced alterations in substrate dependent kinetics of hepatic and renal succinate dehydrogenase (SDH) have been investigated under in vitro conditions. Guanidine hydrochloride (GuHCl) induced a mixed type of inhibition by decreasing the maximal velocity (Vmax) and increasing the Michaelis-Menten constant (Km). The competitive (Ki) and non-competitive (Ki) inhibitory constants were calculated. The values showed that the inhibitory influence of GuHCl is more due to decreased enzyme substrate affinity rather than reduction in the active site density of the enzyme as revealed by low Ki values.

Animals↗
PubMed1997

Influence of partial replacement of butter fat with peanut oil (in infant formula) on erythrocyte fatty acids in infants.

Erythrocyte fatty acid composition was studied in infants fed with three different formulae: formula I containing 20% butter fat; formula II containing 10% butter fat and 10% peanut oil; and formula III containing 10% butter fat and 5% peanut oil with a fat content itself reduced to 15%. The linoleic acid levels were 2.5, 18 and 13% in formula I-III, respectively. Analysis of fatty acids at the time of birth, and 3 and 6 months thereafter, indicated that linoleic acid levels could be improved by supplementation with peanut oil. Arachidonic acid levels (20:4, n-6) did not show a proportional relationship with respect to linoleic acid intake. The other ratio such as triene/tetraene, oleic/linoleic, linoleic/arachidonic and arachidonic/linoleic were all within the normal range, indicating normal desaturase and elongase activity. Thus, our present study suggests that peanut oils could be used for enhancing the linoleic acid levels in infants.

Arachis↗
PubMed1996

Studies on protein quality of green gram (Phaseolus aureus).

Raw green gram contains trypsin inhibitor (5.6 x 10(-3) units/0.1 g) while cooking destroyed the trypsin inhibitor in green gram. No destruction was observed in the case of lysine, methionine, cystine, threonine and tryptophan. Sulphur containing amino acids have been found to be the only limiting amino acids in green gram from studies on blood levels of amino acids and PER experiments. Raw green gram, when fed as a sole source of protein promoted an extremely low growth rate, (13.2 g/4 weeks) and PER (0.77) in rats. Cooking slightly improved the nutritive value of the gram, as indicated by increase in gain in body weight (24 g/4 weeks) as well as PER (1.22). The presence of inhibitors in green gram did not appear to affect some of the essential amino acids as observed from the blood amino acid levels.

Amino Acids↗
PubMed1995

Elaboration of amylase activity and changes in paste viscosity of some common Indian legumes during germination.

The effect of germination upto 120 hours on malting loss, amylase activity and viscosity of nine common Indian legumes was investigated. The amylase activity increased on progressive germination in all legumes, the increase being particularly high for green gram, horse gram, moth bean and black gram. Malted samples had lower cooked paste viscosity than native ones. Samples with high amylase activity exhibited proportionately lower viscosity. Maximum reduction in viscosity was observed in green gram, followed by moth bean, horse gram, black gram and cowpea within 48 hours of germination. Malting losses ranged between 12 to 27 percent over a period of 48 hours in all legumes. Germination beyond 48 hours resulted in considerably higher malting losses without much effect on viscosity.

Amylases↗
PubMed1995

Effect of supplementation of milk fat with peanut oil on blood lipids and lipoproteins in infants.

Three different groups of infants were fed with different formulae based on milk fat. Group I received cow's milk fat formulae with 20% butter fat whereas groups II and III received a formulae which was supplemented with 50 and 33% of peanut oil supplementation in 20% milk fat respectively. Anthropometric measurements, cholesterol, triglyceride, lipoproteins and plasma fatty acids were followed up to a period of 6 months. The results indicated that cow's milk-fed infants had higher cholesterol levels (P < 0.01) than the other two groups. No significant differences with respect to high-density lipoproteins (HDLs) were found, whereas low-density lipoproteins (LDLs) and very low-density lipoproteins (VLDls (VLDls) were found to be increasing up to a period of 6 months. No significant differences were observed with respect to saturated fatty acids and oleic acid (18:1) levels whereas linoleic acid (18:2) clearly showed a proportional relationship between the intake and plasma levels, indicating a positive correlation. Arachidonic acid (20:4) did not, however, show a proprotionate relationship with respect to linoleic acid (18:2) intake. The triene/tetraene, oleic/linoleic, linoleic/arachidonic and total n6 fatty acids were all normal indicating normal activity of desaturase and elongase enzymes for the optimal utilisation of linoleic acid. Thus, the present study suggests that a vegetable oil such as peanut oil could be used in milk fat to improve the essential fatty acid (EFA) status of infants.

Animals↗
PubMed1993

Malignant teratoid medulloepithelioma: a case report.

A case report "Malignant Teratoid Medulloepithelioma" is herewith presented for its rarity. Medulloepitheliomas are embryonic tumors occurring infancy and are probably congenital. Occasionally they show glial tissue and cartilage.

Child, Preschool↗
PubMed1993

Effects of guanidine hydrochloride on catalytic efficiency of glycine amidinotransferase of rat.

Guanidine-induced alterations in substrate-dependent kinetics of glycine amidinotransferase (GAT) have been investigated in homogenates of rat kidney. Guanidine hydrochloride (GuHCl) induced a mixed type of inhibition by decreasing the maximal velocity (Vmax) and increasing the Michaelis-Menten constant (Km). The finding of a value of Ki smaller than that of Ki' denoted that the inhibition of GAT may be due to decreased E to S affinity rather than to reduction in the active site density of the enzyme.

Amidinotransferases↗
PubMed1992

In vitro systems for the analysis of the development of adipose tissue in domestic animals.

In vitro models have been used for many years to assess processes that regulate metabolic activity of adipocytes or that regulate proliferation and differentiation of precursor cells for adipocytes (preadipocytes). The results of these studies have been useful in characterizing the role of various hormones and growth factors in regulating the cellular activities of preadipocytes and adipocytes from domestic animals. Mechanisms that regulate the proliferation and differentiation of the preadipocyte are currently being evaluated with the use of a variety of cell culture systems: matrix suspension, coverslip, micromass, clonal etc. Isolated cell culture of adipose tissue and the tools of molecular biology have allowed direct examination of the effects of potential regulatory hormones of adipogenesis upon gene expression during the development of adipose tissue in domestic animals. Much information has been collected by combining the use of in vivo animal manipulations and in vitro assay techniques to elucidate the mechanisms that affect adipocyte function or formation. The development of in vitro tools to manipulate the development of adipose tissue should result in an increase in our knowledge of the regulation of adipose tissue formation and thus should permit the development of methods to produce leaner and more efficient domestic animals.

Adipose Tissue↗
PubMed1992

Distribution of iodine in marine foods from different regions of India.

The iodine content of fresh and processed marine fish collected from different parts of coastal India was analysed. It was found that Indian marine fish are rich in iodine content (mean of 193 +/- 82 micrograms/100 g on wet weight basis). Also the iodine contents of shell fish and crabs (0.7-2.5 mg/100 g on wet weight basis) were higher than that of other fish. No significant regional differences were noted in the iodine content of marine fish. Further, no differences were observed in case of either salted or sun-dried fish indicating that curing did not influence the iodine content.

Animals↗