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Biomedical subjects

S Ueda

Publications and source records attributed to S Ueda.

At least 973 records · Page 54Linked to original sources
PubMed1983

Hemoglobin Okayama [beta 2 (NA 2) His replaced by Gln]: a new 'silent' hemoglobin variant with substituted amino acid residue at the 2,3-diphosphoglycerate binding site.

A new 'silent' abnormal hemoglobin, Hb Okayama [beta 2 (NA 2) His replaced by Gln], happened to be discovered in a diabetic Japanese female living in Okayama Prefecture, Japan, in the course of glyco-Hb measurement of the blood samples of diabetic patients. This variant did not differ from Hb A by conventional electrophoretic tests. Only the isoelectric focusing on PAG plate for the determination of glyco-Hb and the cation exchanger chromatography were successful in the separation of this abnormal variant from Hb A and glyco-Hb. Functional study of the whole blood demonstrated a slight increase of oxygen affinity.

2,3-Diphosphoglycerate↗
PubMed1983

Hemoglobin Kariya [alpha 40 (C5) Lys leads to Glu]: a new hemoglobin variant with an increased oxygen affinity.

A new abnormal hemoglobin, Hb Kariya [alpha 40 (C5) Lys leads to Glu], with an amino acid substitution at the alpha 1 beta 2 contact was discovered in a young Japanese man. This variant migrated to the anode faster than Hb A, being nearly the same as Hb I in electrophoretic mobility. It amounted to about 6% of the total hemoglobin of the hemolysate. This hemoglobin showed an increased oxygen affinity, decreased heme-heme interaction and a lowered 2,3-DPG effect.

Adult↗
PubMed1983

Reconstitution of heavy chain and light chain 1 in cardiac subfragment-1 from hyperthyroid and euthyroid rabbit hearts.

It is now established that cardiac myosin from hyperthyroid rabbit hearts (TXM) exhibits high Ca2+ ATPase activity. The high Ca2+ ATPase activity of TXM was completely retained in cardiac myosin subfragment-1 (S-1) (1.33 +/- 0.04 mumol Pi/mg per min; euthyroid, 0.51 +/- 0.04). Cardiac S-1 from hyperthyroid and euthyroid rabbits (TXS-1 and NS-1) had the same pattern in SDS-polyacrylamide gel electrophoresis. The possible influence of heavy and light chains of TXM on increasing the ATPase activity was examined by reconstitution in the S-1 preparation. Crosswise reconstitution was performed using cardiac S-1 heavy chain (90,000 daltons) and light chain 1 (LC1) (27,000 daltons) from hyperthyroid and euthyroid hearts. Reconstitution was verified by using radiolabeled LC1. More than 95% of S-1 was recovered with full ATPase activity. When TXS-1 was reconstituted with LC1 from euthyroid hearts, the reconstituted molecule retained high ATPase activity. On the other hand, NS-1 reconstituted with LC1 from hyperthyroid hearts failed to increase the ATPase activity. The ATPase activity of S-1 was determined by the source of the heavy chain. These results suggest that the high Ca2+ ATPase activity of cardiac myosin and S-1 from hyperthyroid animals arises from the molecular alteration of the heavy chain induced by thyroxine administration.

Animals↗
PubMed1983

Serial changes in cytosolic, mitochondrial, and lysosomal enzymes and cardiac myosin light chain II in plasma following coronary ligation in conscious closed-chest dogs.

We studied serial changes in various myocardial enzymes and cardiac myosin light chain II (LCII) in plasma following coronary ligation in 14 conscious closed-chest dogs. Cytoplasmic enzymes [creatine phosphokinase (CPK) and supernatant glutamic oxaloacetic transaminase (sGOT)] reached maximum at 12-24 hr and returned to normal at 72-96 hr. The mitochondrial isozyme of GOT (mGOT) began to rise at 6-9 hr, peaked at 12-30 hr (4.8-42.2 IU/liter), and stayed higher at 96 hr than before infarction. Glutamate dehydrogenase (GLDH), another mitochondrial enzyme, began to elevate at 6-16 hr and reached maximum at 24-60 hr (6.2-20.5 U/liter); GLDH also showed higher levels at 96 hr than before infarction. N-Acetyl-beta-glucosaminidase (NAG), a lysosomal enzyme, showed a biphasic pattern in every case. The first peak appeared at 3-12 hr, and the second one at 36-72 hr. Myosin LCII began to rise at 3-9 hr, peaked at 30-120 hr (34-136 ng/ml), and remained elevated for 7 to 10 days. Determination of these myocardial enzymes or LCII in plasma is useful for the diagnosis of acute myocardial infarction.

Acetylglucosaminidase↗
PubMed1983

Myocardial infarct size from serum cardiac myosin light chain. Clinical and experimental studies.

The relationship between myocardial infarct size and serum cardiac light chain (LC) levels was studied in experimental and clinical myocardial infarction. In dogs with left anterior descending coronary artery occlusion, regression analysis showed good correlation between infarct size and LC II release, but CPK-MB release failed to correlate with infarct size because of a decreasing value of cumulative CPK with larger sized infarctions. In patients with acute myocardial infarction, Peak LC I levels correlated well with CPK release, since the phenomenon of the decreased CPK release in larger sized infarction was not so distinctive in human cases. Thus, LC determination may better quantitate the extent of myocardial damage as well as provide a specific and sensitive method for diagnosis of acute myocardial infarction.

Animals↗
PubMed1983

Identification of serotonin- and vasopressin immunoreactivities in the suprachiasmatic nucleus of four mammalian species.

The distribution of serotonin- and vasopressin immunoreactivities in the suprachiasmatic nucleus (SCN) of four mammalian species was studied with the use of the modified peroxidase-antiperoxidase (PAP) method and antisera to serotonin and vasopressin. In the SCN of the rat, hamster and cat, we noted a large number of serotonin-immunoreactive nerve fibers particularly in the ventral area, where these fibers containing small varicosities (less than 1 micron in diameter) formed a dense plexus. In the monkey (Macaca fuscata), however, only few serotonin-containing fibers were evident throughout the SCN. Vasopressin-immunoreactive somata and fibers were distributed in large numbers in the SCN of the rat, hamster, cat and monkey, especially in the dorsal nuclear area. Regional and species-related differences of serotonin- and vasopressin distribution in the SCN were elucidated; possible functional differences between the ventral and dorsal areas of the SCN are discussed.

Animals↗
PubMed1983

Immunohistochemical demonstration of serotonin nerve fibers in the hypothalamus of the cat.

Distribution of serotonin nerve fibers in the hypothalamus of the cat was studied using the peroxidase antiperoxidase (PAP) immunohistochemical method. There was a heavy concentration of serotonin nerve fibers in the nucleus suprachiasmaticus, the nucleus ventromedialis and the nucleus dorsomedialis. The distribution pattern of the serotonergic fibers in the cat was principally similar to that of the rat and monkey. However, species differences were noted in the mamillary complex, the nucleus hypothalamicus anterior, the nucleus paraventricularis and the nucleus supraopticus.

Animals↗
PubMed1983

Immunohistochemical demonstration of serotonin neurons in the central nervous system of the turtle (Clemmys japonica).

The location of the somata, course of the main tracts, and fiber distribution of the serotonin neurons in the turtle brain were studied using the peroxidase-antiperoxidase (PAP) immunohistochemical method with antibodies against serotonin (5-hydroxytryptamine). The somata of the serotonin neurons were distributed in the reticular formation of the brainstem from the mesencephalon to the lower medulla level and in a resticted region of the hypothalamus, viz. the paraventricular organ (PVO). In the PVO the serotonin neurons were seen to have the appearance of cerebrospinal fluid-contacting neurons. Analysis of serial sections cut in the frontal and sagittal planes revealed a widespread distribution of the serotonin immunoreactive fibers in the turtle brain. Prominent concentrations of the serotonin immunoreactive fibers were found in the lateral portion of the striatum, the ventral portion of the septum, the nucleus corporis geniculati lateralis, the nucleus pretectalis, the nucleus isthmi parvocellularis, the optic tectum, and the lateral edge of the reticular formation of the brainstem. Ascending and descending serotonin pathways could be defined: the ascending pathway originated mainly from the nucleus profundus mesencephali caudalis, nucleus lemnisci lateralis, nucleus reticularis isthmi and, less prominently, from the nucleus raphe superior pars lateralis, and the descending pathway arose predominantly from the nucleus raphe inferior. The fibers of the ascending pathway projected widely in the prosencephalon and mesencephalon, via the medial forebrain bundle. The descending pathway ran through the ventral and lateral portion of the medulla oblongata and spinal cord.

Animals↗
PubMed1983

Immunohistochemical demonstration of serotonin-containing CSF-contacting neurons in the submammalian paraventricular organ.

The distribution and morphological aspects of the serotonin-containing neurons in the paraventricular organ of the carp, frog, turtle and chicken were studied by means of an immunoperoxidase technique using serotonin antiserum. In all species the serotonin-containing neurons were seen to have the appearance of the CSF-contacting neurons and to be distributed in the pars ependymalis and the pars hypendymalis of the organ. Particularly, in the frog, the serotonin-containing CSF-contacting neurons, mostly bipolar in shape, were also observed in the pars distalis. Their proximal processes protruded into the ventricular lumen through the ependymal layer with a globular- and triangular-shape. The distal processes projected ependymofugally to the pars distalis and formed a fine plexus in the neuropil of this part. The density of the serotonin fibers in the pars distalis was greater in the carp than in the other species.

Animals↗
PubMed1983

Structure of the human immunoglobulin C epsilon 2 gene, a truncated pseudogene: implications for its evolutionary origin.

Cloning of the overlapping DNA fragments together with Southern hybridization experiments showed the organization of the human C epsilon and C alpha gene cluster as 5'-C epsilon 2-14 kilobases-C alpha 1----C epsilon 1-13 kilobases-C alpha 2-3'. Comparison of the nucleotide sequences of the C epsilon 1 and C epsilon 2 genes revealed that four deletions have taken place in the C epsilon 2 gene and its flanking regions. The three deleted regions in the 5' side of the C epsilon 2 gene are partially filled with shorter inserted sequences. One of them has removed the CH1 and CH2 exons and a portion of the epsilon switch (S epsilon) region. The S epsilon region and the CH4 exon still retain the functional structures, whereas the CH3 exon has been inactivated by deleting its 5' intervening sequence necessary for splicing. The tetranucleotide T-G-G-G (or T-G-G-C), which is usually found in close proximity of the class-switch recombination sites in mouse myelomas, is located 5' to the three deletion sites. The results imply that the mechanism responsible for the heavy chain class-switch recombination might be relevant to the evolutionary mechanism of creation of the truncated C epsilon 2 gene. The other deletion in the 3' flanking region of the C epsilon 2 gene may be due to slipped mispairing of the short direct repeat (C-C-C-C-C) at both ends.

Base Sequence↗
PubMed1983

Monoclonal antibodies reactive with the surface and secreted glycoproteins of Marek's disease virus and herpesvirus of turkeys.

Hybridomas were formed between mouse myeloma cells and spleen cells from mice immunized with Marek's disease virus (MDV) or with herpesvirus of turkeys (HVT). Three monoclonal antibodies were obtained, two (M26 and M34) from MDV clones and one (H9) from an HVT clone, all of which were specific for cross-reactive membrane antigen (MA) expressed on the surface of cells infected with MDV or HVT. All three antibodies also reacted with MDV- and HVT-specific glycoproteins in the molecular weight (mol. wt.) ranges 54K to 70K (MDV-gp54/70) and 50K to 64K (HVT-gp50/64), respectively. These glycoproteins constitute the putative 'A' antigens which are found in the medium of cultures infected with MDV or HVT. These results suggest that the cross-reactive MA may correspond to 'A' antigen. Pulse-chase experiments using monoclonal antibodies revealed the presence in virus-infected cells of precursor and processed forms of MDV-gp54/70 and HVT-gp50/64 which differ in size. Moreover, by two-dimensional gel electrophoresis we found that MDV and HVT glycoproteins were separated to heterogeneous spots by electric charge as well as mol. wt. The several spots with higher mol. wt. and with more acidic isoelectric points among them were lost by treatment with neuraminidase, suggesting that the processing was, at least in part, due to the addition of sialic acid to the precursor forms. Tunicamycin blocked the surface expression of cross-reactive HVT-MA on HVT-infected cells. Phosphonoacetic acid inhibited both the appearance of HVT-MA on the cell surface and synthesis of HVT-gp50/64, indicating that the MA and secreted glycoprotein were late gene products of the HVT genome.

Animals↗
PubMed1983

Most virus-specific polypeptides in cells productively infected with Marek's disease virus or herpesvirus of turkeys possess cross-reactive determinants.

Most virus-specific polypeptides in cells productively infected with Marek's disease virus (MDV) or herpesvirus of turkeys (HVT) possess cross-reactive antigenic determinants, although the two viruses have very little DNA homology. The cross-reactivity appeared to be more evident when [35S]methionine-labelled polypeptides were immunoprecipitated than when the [3H]glucosamine-labelled polypeptides were immunoprecipitated, suggesting that the glycoproteins of MDV and HVT may be less related in structure than other proteins. The major cross-reactive glycoproteins excreted into the culture mediums of MDV- and HVT-infected cells had molecular weights of 64 000 (gp64) and 56 000 (gp56) respectively.

Animals↗
PubMed1983

Immunohistochemical studies on carcinoembryonic antigen in adenocarcinomas of the uterus.

In order to distinguish endocervical adenocarcinoma from endometrial adenocarcinoma, an immunoperoxidase stain for carcinoembryonic antigen (CEA) was tried. All of 10 endocervical adenocarcinomas revealed CEA and an adenosquamous carcinoma in the uterine cervix also showed it, while a mesonephroid adenocarcinoma in the uterine cervix did not. The immunohistochemical reaction products for the antigen were not observed in the glandular structures of 20 endometrial adenocarcinomas, although CEA was detected in all foci of squamous epithelial metaplasia occurred within 7 endometrial adenocarcinomas. CEA was detected in the endocervical type of glandular epithelium within a special endometrial adenocarcinoma containing predominantly endocervical type glandular epithelium. The immunoperoxidase staining pattern for CEA in the endocervical adenocarcinoma was related to the degree of histological differentiation of tumors, as follows; in the well differentiated glandular structure CEA was located on the luminal surface, while it was detected in the whole cytoplasm of tumor cells within the moderately and poorly differentiated areas. In conclusion, the immunoperoxidase stain for CEA would be useful for estimating malignancy of glandular structures within the uterus, distinguishing endocervical adenocarcinoma from endometrial adenocarcinoma, and grading of histological differentiation of endocervical adenocarcinoma.

Adenocarcinoma↗
PubMed1983

Evaluation of methods for estimating infarct size by myosin LC2: comparison with cardiac enzymes.

The relationship between histologically determined infarct size and release or peak levels of circulating cardiac enzymes and myosin light chain 2 (LC2) was studied. Myocardial infarction was produced by ligating the left anterior descending coronary artery in 18 conscious closed-chest dogs. Creatine phosphokinase (CPK), cytosolic and mitochondrial isozymes of aspartate transaminase (sAST and mAST) in the plasma, and LC2 in the serum were measured serially until 10 days after infarction, when infarct size was determined histologically [range 4.0-38.8% of the left ventricular weight (%LV)]. Infarct size correlated most closely with LC2 release (r = 0.82, P less than 0.001) and less closely with peak sAST (r = 0.59, P less than 0.01), peak mAST (r = 0.49, P less than 0.05), peak CPK (r = 0.22), and CPK release (r = 0.14). The correlation between infarct size and CPK release was improved by limiting the analysis to the dogs with infarct size of less than 20% LV (n = 11, r = 0.53, P less than 0.1). Because, among cardiac enzymes and LC2, CPK activity decayed most rapidly in the lymph fluid when incubated in vitro, degeneration of CPK in the lymph stream may contribute to the nonlinear relationship between infarct size and CPK release.

Animals↗
PubMed1983

Two types of oxytocin and vasopressin nerve fibers in the intra- and extrahypothalamic neuronal systems as revealed by immunohistochemistry.

The oxytocin and vasopressin nerve fibers in the intra- and extrahypothalamic neuronal systems of several mammalian brains are immunohistochemically demonstrated using a modified peroxidase-antiperoxidase technique. The axonal processes of these peptidergic neurons are classified into thick and thin beaded fibers. Thick beaded fibers were preferentially distributed in the hypothalamo-neurohypophysial tract and in some circumventricular organs, with termination on the blood vessels. Thin beaded fibers were found in various extrahypothalamic areas and these terminals were in the vicinity of the neuronal somata of such areas. This report suggests that there are at least two different functions concerning neurotransmission in the oxytocin and vasopressin neuronal system.

Animals↗
PubMed1983

Effects of some prostaglandins on urinary bladder and urethra isolated from the dog.

Effects of prostaglandins (PGs) E1, E2, E2 and F2 alpha on the vesical and urethral smooth muscles of dogs were isometrically investigated in vitro. In vesical smooth muscles, 10(-8)-10(-6) PGE1/ml produced biphasic responses in the dome preparations; relaxation at 10(-8) g/ml and contractions at 10(-7)-10(-6) g/ml. In the trigone preparations, 10(-8)-10(-6) g PGE1/ml caused dose-dependent contractions. 10(-8)-10(-6)M g PGE2/ml and 10(-7)-10(-6) g PGF2 alpha /ml induced dose-dependent contractions of the dome and the trigone preparations. In urethral smooth muscles, 10(-8)-10(-6) g PGE1/ml and 10(-8)-10(-6) g PGE2/ml produced dose-dependent relaxations, while 10(-8)-10(-6) g PGF2 alpha /ml caused dose-dependent contractions. Results indicate that PGE, particularly PGE2, is able to stimulate micturition through vesical contraction and urethral relaxation.

Alprostadil↗