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Biomedical subjects

S Ueda

Publications and source records attributed to S Ueda.

At least 865 records · Page 48Linked to original sources
PubMed1986

Effects of prostaglandin E2 and I2 on renal cortical and medullary blood flow in rabbits.

The effects of intra-aortic administrations of prostaglandin (PG) E2 and I2 on the renal regional blood flow were investigated in rabbits. Mean arterial blood pressure (MABP) was monitored by the direct method and total renal blood flow (TRBF) was measured using the electromagnetic method. Renal cortical and medullary blood flows (RCBF and RMBF) were measured by the hydrogen gas clearance method. Both PGE2 and PGI2 caused a dose-dependent reduction of MABP. At doses having no significant effect on MABP, PGE2, and PGI2 increased TRBF and decreased renal vascular resistance (RVR). The maximum reduction in RVR induced by PGI2 was significantly greater than that by PGE2. PGE2, produced significant increases in RCBF and in particular in RMBF, while PGI2 induced similar changes in RCBF and RMBF. The results suggest that PGE2, which is a weaker vasodilator than PGI2, mainly acts as a medullary vasodilator, and that PGI2 has similar vasodilatory effects on the cortex and medulla.

Animals↗
PubMed1986

Increased serum IgE antibodies in institutionalized asthmatic children after a transient return home. The role of house dust mite allergens in the home as a trigger of asthmatic attacks in mite-sensitive patients.

Increased serum IgE antibodies to Dermatophagoides pteronyssinus, a house dust mite, were observed in 12 of 13 institutionalized mite-sensitive asthmatic children after returning for four days to their own homes. These results implicate the house dust mite as a trigger of asthmatic attacks in these patients.

Adolescent↗
PubMed1986

A long-term follow-up study on the efficacy of further attenuated live measles vaccine, Biken CAM vaccine.

Antibody persistence was measured in 39 children in an open community 12-13 years after immunization against measles with further attenuated live vaccine, Biken CAM. Serum samples of the children taken every two or three years after vaccination had higher, lower, or the same HI antibody titers as those in samples taken 6 weeks after vaccination. These differences reflected a decrease in the titer in some children and subclinical natural reinfection in others. However, all the children still retained detectable antibody in 12 or 13 years after vaccination, indicating long-term persistence of immunity after immunization with Biken CAM vaccine. For evaluation of the protective efficacy of the vaccine, matched controls were studied during the same period. Serological examination revealed that 97.5% of the controls were infected with measles and contracted the disease. In contrast, none of the vaccinees developed clinical infection after close contact with measles patients.

Adolescent↗
PubMed1985

Increases in cytosolic free Ca2+ induced by ATP, complement and beta-lipoprotein in mouse L fibroblasts.

By means of Ca2+- and K+-selective microelectrodes, the changes in intracellular free Ca2+ and K+ were measured during the hyperpolarizing responses induced by ATP, complement and beta-lipoprotein in mouse fibroblastic L cells. The cytoplasmic Ca2+ concentration [( Ca]i) was about 0.4 microM in the resting state. The hyperpolarizing responses always coincided with a phasic increase in [Ca]i. ATP or beta-lipoprotein induced about a 2-fold rise in [Ca]i, and complement did up to 3-fold. Both the hyperpolarizing responses and [Ca]i increases were prevented by removal of external Ca2+ or by application of a Ca-channel blocker, nifedipine. Quinine, a Ca-activated K-channel inhibitor, suppressed the hyperpolarizing responses but not the [Ca]i increases. During the hyperpolarizing response, the intracellular free K+ concentration gradually decreased from about 120 to 110 mM. Thus, it is concluded that ATP, complement and beta-lipoprotein caused a transient elevation of cytoplasmic free Ca2+ due to Ca2+ influxes, thereby inducing electrical membrane responses through activation of Ca-dependent K-channels in the fibroblasts.

Adenosine Triphosphate↗
PubMed1985

Identification of Marek's disease virus-specific antigens in Marek's disease lymphoblastoid cell lines using monoclonal antibody against virus-specific phosphorylated polypeptides.

For identification of the antigens specific to Marek's disease virus (MDV) in virus-non-producer lymphoblastoid cell lines established from a tumor of Marek's disease (MD), hybridomas producing monoclonal antibodies (MAbs) against the antigens were isolated. Immunogens for preparation of the hybridomas were purified from the lysate of an MD-lymphoblastoid cell line, MSB1, by affinity chromatography coupled with chicken anti-MDV serum immunoglobulin G. Three of the MAbs obtained, MB1, MB2 and MB3, were specific to MDV by immunofluorescence test. An immunofluorescence test using MB2 antibody showed that immunofluorescence-positive cells in non-producer MD-lymphoblastoid line cells became detectable when the culture temperature was shifted from 41 degrees C to 33 degrees C or when treatment with 5-iodo-2-deoxyuridine (IUdR) was performed, indicating that the antigen reactive with MB2 antibody is an MDV-specific early antigen. This temperature shift or IUdR-treatment did not induce other MDV-specific antigens, such as late gene products of MDV, detected with MAbs. MB1 and MB2 antibodies immunoprecipitated 4 MDV-specific phosphorylated polypeptides with molecular weights (MWs) of 43,000 (43kd), 39kd, 36kd and 24kd from chick embryo fibroblasts productively infected with virulent MDV. In the place of 43kd, phosphorylated 44kd polypeptide was precipitated from avirulent MDV-infected fibroblasts. However, MB3 antibody did not precipitate any MDV-specific polypeptides from infected fibroblasts. These results suggest that the phosphorylated polypeptides are MDV-specific polypeptides predominantly expressed in non-producer MD-lymphoblastoid cell lines.

Animals↗
PubMed1985

Immuohistochemical demonstration of alpha-atrial natriretic plypeptide-containing neurons in the rat brain.

Using the immunoperoxidase technique in conjunction with specific antisera to alpha-atrial natriuretic polypeptide (alpha-ANP), it was shown that immunoreactive cell bodies and varicose fibers are widely distributed throughout the rat brain. The highest concentrations of alpha-ANP-containing neuronal cell bodies and fibers were found in the hypothalamus and septum. This result confirms the radioimmunological determination of alpha-ANP immunoreactivity in the rat brain.

Animals↗
PubMed1985

Immunohistochemical demonstration of serotonin nerve fibers in the corpus striatum of the rat, cat and monkey.

The distribution of serotonin-containing nerve fibers in the corpus striatum of the rat, cat and monkey was studied with modified peroxidase-antiperoxidase method using serotonin antiserum without any pretreatment. In the neostriatum (caudate nucleus and putamen) of all mammalian species investigated, the immunoreactive fibers were distinct varicose fibers in a fine network. The concentration of these fibers was high in the ventral, medial and caudal neostriatum. Especially in the area bounded by the globus pallidus, serotonin fibers were abundant and compactly arranged along the nucleus. In this area of the monkey, a few thick fibers (tract fibers) were intermingled; they ran along the lateral medullary lamina. Such tract fibers were also observed outside the medial medullary lamina and in the central portion of the medial pallidal segment. The paleostriatum (globus pallidus and entopeduncular nucleus) of the rat and cat as well as the medial pallidal segment of the monkey was diffusely innervated with serotonin fibers composed of numerous varicosities and fine intervaricose segments, while in the lateral pallidal segment of the monkey, the distribution of fibers was scanty and partial. Our results hint at the morphological basis of the serotonergic regulation of the extrapyramidal system in mammals.

Animals↗
PubMed1985

The organization of serotonin fibers in the mammalian superior colliculus. An immunohistochemical study.

The distribution of serotonin immunoreactivity in the superior colliculus (SC) of the rat, hamster, chipmunk, cat, and monkey was studied using a sensitive immunohistochemical method. In all of these animals, serotonin immunoreactivity formed a dense network of varicose fibers throughout the SC. These fibers had a characteristic arrangement corresponding to the laminar structures of the SC. Except in the chipmunk, serotonergic fibers were more dense in the stratum griseum superficiale than in the other layers. In the SC of the chipmunk, these fibers appeared evenly distributed. To explore the degree of serotonergic innervation in each layer, a semi-quantitative assay of serotonin immunoreactive varicosities was conducted in the rat, chipmunk, cat, and monkey. Peaks in varicose density were seen in the stratum griseum superficiale, the stratum griseum intermedium and the stratum griseum profundum. In the rat, cat, and monkey, the highest density of these varicosities was in the stratum griseum superficiale. On the other hand, the stratum griseum intermedium of the chipmunk SC received the greatest innervation of serotonergic varicose fibers.

Animals↗