Search PubMed⌕ Search

Biomedical subjects

S Tsuboi

Publications and source records attributed to S Tsuboi.

180 records · Page 10Linked to original sources
PubMed1975

Further studies on an eleventh case of heavy (Hgamma1) chain disease--clinical studies.

An eleventh case of heavy (Hgamma1) chain disease (Yok), surviving for more than 10 years and still living showed clinical and pathological findings similar to cases described in the past. The patient was given only glucocorticosteroids, ACTH, antibiotics and gamma globulin, as specific drugs. Precipitation arcs besides the major ones formed by albumin and Fc fragment were disclosed by immunoelectrophoresis. The existence of these minor components were confirmed with antigen-antibody crossed electrophoresis and Sephadex G-200 gel filtration. They did not form precipitation arcs with the other antigens available and they appeared in the same fractions of IgG on gel filtration suggesting their having higher molecular weight than the major ones. In addition to these findings, the clinical course of the patient is described.

Adult↗
PubMed1975

Further studies on an eleventh case of heavy (Hgamma1) chain disease--physico-chemical studies.

An abnormal protein with similar antigenic properties to Fc fragments of IgG, was found in the serum and urine of an eleventh case of heavy (Hgamma1) chain disease (Yok). This protein was purified with ammonium sulfate precipitation and by column chromatography of DEAE cellulose, CM cellulose and Sephadex G-200. The purity of the protein obtained was 98.5%. It was crystallized easily, forming thin hexagonal plates of various sizes. The chemical compositions and physical properties of the protein including viscosity, partial specific volume, diffusion constant, sedimentation constant, frictional ratio, extinction coefficient and iso-ionic point are reported.

Adult↗
PubMed1975

Further studies on an eleventh case of heavy (Hgamma1) chain disease--biosynthetic studies.

In vitro quantitative biosynthetic studies were carried out on bone marrow cells obtained from an eleventh case with gamma heavy chain disease. The findings indicate that neither cytoplasmic nor extracellular degradation was responsible for the presence of the gamma heavy chain fragment in serum. The absence of a covalent-bound light chain was also confirmed.

Adult↗
PubMedDate not supplied

Intra-oral fluoride retention 3 minutes after fluoride mouthrinsing in 4- to 5-year-old children: effects of fluoride concentration and rinsing time.

This study was undertaken to explore the site-specific retention rate and the possibility of shortening fluoride (F) mouthrinsing time of kindergarten children. Fluoride retention after 10-, 20- and 30-second mouthrinsing was determined in 43 kindergarten children aged 4-5 years. Tooth surfaces were sampled by a paper point method. Fluoride concentrations in the salivary film on tooth surfaces increased from primary molars to primary incisors in the maxilla and decreased from primary molars to primary incisors in the mandible. The fluoride solution reached the primary molars even after a 10-second rinse, but F concentrations were higher after 20 s than after 10 s and significantly higher after 30 s than after 10 s. No significant difference was observed between 20 and 30 s. The average total F retained in the mouth was 0.13 mg after 20 s and 0.17 mg after 30 s. It was concluded that 30-second mouthrinsing, which is used extensively in Japanese kindergartens, can be shortened to 20 s.

Analysis of Variance↗
PubMedDate not supplied

Use of fluoridated dentifrice and glucose retention at the approximal areas of anterior teeth.

The present study was undertaken to learn to what extent the use of dentifrice with fluoride affected glucose retention after glucose rinsing. Fifty-eight subjects consisting of forty male adults and eighteen female adults whose average age was 21.9 years were divided at random into two groups; one group used fluoridated dentifrice and the other group used nonfluoridated dentifrice. Subjects rinsed their mouths for fifteen seconds with 20 mL of 0.5 M glucose solution. Samples of saliva were taken from the approximal areas between the right and left central incisors of the maxilla and mandible by inserting the tip portion of a paper point three-minutes after the glucose rinsing, sample of saliva were taken from the neighboring approximal areas of the left central and left lateral incisors. Glucose content of the paper points was determined using the enzyme membrane method. Determinations were made before and at the end of the first, third, and fifth month over a five-month period. Fluoride concentrations in the resting saliva were also determined in different months during this period. At three-minutes and six-minutes, glucose retention in the group using fluoridated dentifrice was found to decrease slightly throughout the experiment; while glucose retention in the group on nonfluoridated dentifrice leveled off or increased in the fifth month. Fluoride concentration in the resting saliva increased during the experiment in the group using fluoridated dentifrice; while fluoride concentration in the group using nonfluoridated dentifrice did not change. It was concluded that the regular use of fluoridated dentifrice made glucose retention slightly lower and fluoride level in whole saliva higher.

Adult↗
PubMedDate not supplied

Astigmatic and refractive stabilization after cataract surgery.

We assessed the timing of astigmatic and refractive stabilization following six cataract surgery procedures with intraocular lens implantation in 229 eyes divided into six groups in the following incision sizes and methods of wound closure: 11-mm incision with running suture closure (26 eyes) 6.5-mm incision with running suture closure (29 eyes) 6.5-mm incision with single horizontal suture closure (25 eyes) 6.5-mm incision without suture closure (46 eyes) 5.5-mm incision without suture closure (51 eyes) 3.2-mm incision without suture closure (52 eyes) Analyzed up to 6 months postoperatively were: the mean and standard deviation of axis-based keratometric cylinders the absolute value of the induced-cylinder vector the spherical equivalent of the refractive power. In the 11- and 6.5-mm incision running suture groups, these parameters did not stabilize during the study period. In the 6.5-mm incision horizontal suture and sutureless groups, the values stabilized at 3 months postoperatively; in the 5.5-mm incision group, at 1 month; and in the 3.2-mm incision group, at 2 weeks. These results indicate that the appropriate point at which to prescribe postoperative correction spectacles differs significantly depending on the procedure, and that smaller incisions with wound-closure methods that do not exert vertical force are associated with fewer postoperative refractive changes.

Aged↗