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S Toma

Publications and source records attributed to S Toma.

At least 127 records · Page 7Linked to original sources
PubMed1984

Legionella bozemanii serogroup 2: a new etiological agent.

A newly discovered bacterium, Toronto 3, isolated from a lung aspirate of a patient with pneumonia, has been characterized. The isolate was identified as Legionella bozemanii by chemical data from cellular fatty acid and ubiquinone analyses and by DNA relatedness studies. The isolate, however, differs phenotypically from L. bozemanii by its colonial characteristics and strong interspecies serological cross-reactions, which are unique among clinical isolates of legionellae. The name L. bozemanni serogroup 2 is proposed. The reference strain is Toronto 3. The clinical illness caused by L. bozemanii serogroup 2 was not distinguishable from other Legionella infections. L. bozemanii is the third Legionella species with more than one serogroup. Rapid laboratory diagnosis of this strain by direct fluorescent antibody test may be complicated in the absence of culture isolation.

DNA, Bacterial↗
PubMed1984

O:13a,13b, a new pathogenic serotype of Yersinia enterocolitica.

Forty strains of a new Yersinia enterocolitica serotype isolated in the United States from nonhuman primates and humans were characterized as serotype O:13a,13b. Most of the human strains were isolated from a large multistate outbreak of milkborne enteritis. A common antigenic factor between this new serotype and four previously described Y. enterocolitica (O:7,13; O:18; O:44; and O:44,45) led to recharacterization of the latter as serotypes O:7,13a,13b; O:18,13b; O:44,13a; and O:44,13a,45.

Agglutination↗
PubMed1984

A new Legionella species, Legionella feeleii species nova, causes Pontiac fever in an automobile plant.

From 15 to 21 August 1981, Pontiac fever affected 317 automobile assembly plant workers. Results of serologic tests were negative for Mycoplasma, Chlamydia, respiratory tract viruses, and previously described legionellae. A gram-negative, rod-shaped organism (WO-44C) that did not grow on blood agar, required L-cysteine for growth, and contained large amounts of branched-chain fatty acids was isolated from a water-based coolant. The organism did not react with antisera against other legionellae, and on DNA hybridization the organism was less than 10% related to other Legionella species. Geometric mean titers found by indirect fluorescent antibody testing to WO-44C were significantly higher in ill employees than in controls (p = 0.0001). Attack rates by department decreased linearly with the department's distance from the implicated coolant system. The etiologic agent apparently was a new Legionella species; we propose the name Legionella feeleii species nova (AATC 35072). This is the first outbreak of nonpneumonic legionellosis in which the etiologic agent is not L. pneumophila, serogroup 1.

Aerosols↗
PubMed1983

[Clinical study of a prepared reducing diet: adequacy of the vitamin-mineral contribution in comparison to the nutritional and immunity status].

The Authors studied the effectiveness and safety of a commercial hypocaloric diet on 11 obese postmenopausal women. During the experimental period 1 meal/day has been replaced with a chemically defined low calories product. Different parameters have been evaluated to assess the nutritional status 1), anthropometric: weight, skinfold thickness, arm muscle circumference; 2) biochemical total plasma proteins, transferrin, vitamins A, E, C, B12, folic acid; plasma iron, hemoglobin, MCV, RBC; 3) immune status (T lymphocytes and immunoglobulins). Moreover blood sugar, cholesterol and triglyceride levels, as well as blood pressure have been taken into account. All the possible side effects and the diet acceptance for all the patients have been scored. After 1 month all the abnormal conditions (weight, cholesterol, blood pressure) improved, while the nutritional status and immune response remained at an optimal level.

Aged↗
PubMed1982

Infection caused by Yersinia enterocolitica serotype O:21.

Yersinia enterocolitica serotype O:21, biotype 3 (Wauters) was isolated from the appendix and stool of a 7-year-old girl. The same organism was later isolated in pure culture from pus from a postoperative wound infection in this patient. She developed a significant serological response (titer of 800). There was thus strong clinical evidence of pathogenicity associated with this rather uncommon human serotype. Laboratory studies in vitro and in vivo pathogenicity showed that the organism was autoagglutinable, Serény test positive, and HeLa cell invasive; when given orally to mice, it produced diarrhea and subsequently death. The results of laboratory studies of virulence correlated closely with the clinical evidence of pathogenicity in this case.

Animals↗
PubMed1982

Detection of Legionella antigenuria by reverse passive agglutination.

A reverse passive agglutination method was developed to detect soluble antigens of Legionella spp. By this method Legionella antigens were detected in urine specimens from 14 of 15 antigenuric patients with clinically diagnosed Legionnaires disease and in none of 263 urine samples from healthy subjects or patients with urinary tract infections. Intra-genus cross-reactivity was observed only between L. pneumophila serogroups 2, 3, and 6. The Legionella reverse passive agglutination method was also evaluated with reference to reagent concentrations, test conditions, and subjectivity of reading test results. The method is rapid and does not require special equipment.

Agglutination Tests↗
PubMed1981

Cleavage of the (1 goes to 3)-2-acetamido-2-deoxy-beta-D-glucopyranosyl linkage present in keratan sulfate. The A and B isoenzymes of human liver hexosaminidase (EC 3.2.1.30).

The disaccharide 2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1 goes to 3)-D-[1-3H]-galactitol, prepared from keratan sulfate, was rapidly hydrolyzed by the A and B isoenzymes of normal human liver hexosaminidase (EC 3.2.1.30), and by the B isoenzyme prepared from the liver of a patient who had died of Tay-Sachs disease. The disaccharide substrate was also hydrolyzed by extracts of normal, cultured-skin fibroblasts, and fibroblasts of patients with Tay-Sachs disease, whereas it was not hydrolyzed by fibroblast extracts of patients with Sandhoff disease. Thus, effective degradation of keratan sulfate, secondary to a defect of the beta subunits present in the A and B isoenzymes of hexosaminidase, may contribute to the appearance of skeletal lesions in patients affected by Sandhoff disease.

Acetylglucosaminidase↗
PubMed1981

Preparation from keratin sulfate of substrates for the measurement of 2-acetamido-2-deoxy-D-glucose 6-sulfate sulfatase and (1 goes to 3)-N-acetyl-beta-D-glucosaminidase.

An extract of bacterial cells Pseudomonas sp. IFO-13309 grown on medium containing 0.1% bovine cornea keratan sulfate of low sulfate content degraded exhaustively bovine cornea keratan sulfate to give 2-acetamido-2-deoxy-beta-D-gluco-pyranosyl 6-sulfate-(1 goes to 3)-D-galactose, isolated by gel filtration on Sephadex G-25 and purified by preparative paper chromatography. This was reduced with sodium borotritide to give 2-acetamido-2-deoxy-beta-D-glucopyranosyl 6-sulfate-(1 goes to 3)-D-[1-3H]galactitol, purified by gel filtration on Sephadex G-15, which was an excellent substrate for the measurement of 2-acetamido-2-deoxy-D-glucose 6-sulfate sulfatase. The reduced, radioactive monosulfated disaccharide was desulfated with methanolic 70mM hydrogen chloride and purified by gel filtration on Sephadex G-15 to give O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-(1 goes to 3)-D-[1-3H]galactitol, which allowed the measurement of (1 goes to 3)-N-acetyl-beta-D-glucosaminidase. This enzyme may participate in the normal degradation of keratan sulfate.

Acetylglucosaminidase↗