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Biomedical subjects

S Tani

Publications and source records attributed to S Tani.

At least 163 records · Page 9Linked to original sources
PubMed1985

A comparative study of two kinds of cathepsin D-type proteinases from rat gastric mucosa.

The localization of cathepsin D-like acid proteinase in the rat stomach and other tissues was studied, and its biochemical properties were compared with those of rat gastric cathepsin D (EC 3.4.23.5). Cathepsin D-like acid proteinase existed overwhelmingly in the mucosal layer and was hardly detected in the gastric juice. Its subcellular distribution profile was very similar to that of acid phosphatase, but not to that of pepsinogen. This proteinase-like enzyme activity was also found in rat splenic extract. These results strongly suggest that the proteinase is a lysosomal enzyme. In addition, cathepsin D-like acid proteinase demonstrated an in vitro transition of molecular species during storage at -30 degrees C. Although this molecular change was distinctive in ion-exchange column chromatography and susceptibility to some enzyme inhibitors, it was not accompanied by a significant decrease in molecular weight. To compare cathepsin D-like acid proteinase with ordinary cathepsin D, gastric cathepsin D was newly purified to apparent homogeneity in polyacrylamide gel electrophoresis. Its biochemical properties demonstrate that this is a true cathepsin D in rat gastric mucosa. Moreover, this cathepsin D activity was not abolished by treatment with antiserum specific to cathepsin D-like acid proteinase or pepsinogen. From these results, we can conclude that the proteinase is a lysosomal acid proteinase different from newly purified gastric cathepsin D.

Animals↗
PubMed1985

Effects of gastrointestinal hormones and their related compounds on gastric motility in the rat.

We examined the effects of certain gastrointestinal hormones on gastric motility using rat stomach preparatios in vivo. Changes of water level caused by the movement of the stomach which was filled with saline were recorded. Single injections of cholecystokinin (1, 2 and 4 micrograms/kg) induced relaxation of the stomach. Single injections of bombesin in low doses (below 0.2 microgram/kg) induced relaxation and in high doses (over 0.2 micrograms/kg) contraction after brief relaxation. Single injections of neurotensin (1, 2, 4 and 8 micrograms/kg), somatostatin (5, 10 and 20 micrograms/kg) and substance P (1, 2, 4 and 8 micrograms/kg) induced relaxation followed by contraction, but their dose-response relations were obscure. Infusions of neurotensin (1, 5 and 25 micrograms/kg/h) and somatostatin (2.5 and 5 micrograms/kg/h) enhanced the stomach tension, whereas substance P (1, 5 and 25 micrograms/kg/h) reduced it. Single injections and infusions of neurotensin, somatostatin or substance P showed different effects on gastric motility. On the other hand, Met-enkephalin (1, 10 and 100 micrograms/kg) and porcine motilin (1, 10 and 100 micrograms/kg) did not affect gastric motility in our rat stomach preparations. These results suggest that some gastrointestinal hormones take part in stomach movements.

Animals↗
PubMed1985

Determination of localization of allergen and IgG inducing antigen in Dirofilaria immitis by immunofluorescent antibody method.

More detail studies were made into the localization of IgE inducing antigen (allergen) and IgG inducing antigen in the body of adult male and female Dirofilaria immitis worms by the indirect immunofluorescent antibody method using rat sera against purified allergen and IgG inducing antigen. Allergen was detected in large amounts in the body cavity fluid and the excretory canal, and it was also detected in the cuticles and the lateral lines in the vicinity of the excretory canal. IgG inducing antigen was found in the largest amount in the site of musculature inside of cuticle. Besides the musculature, it was also recognized in smaller amounts in the muscle layer, the body cavity fluid and the excretory canal. In females it was also detected on the surface of microfilaria in the uterus. As to allergen as well as IgG inducing antigen, the fluorescence was recognized most strongly in the central part of the worm body, and it tended to diminish gradually as it approached the head and tail. The concentration of allergen determined by the immunofluorescent antibody method was higher in males than in females. Especially in the excretory canal, a difference was seen between males and females. Allergen was thought to be secreted mainly out of the excretory canal and present largely in the body cavity fluid. On the other hand, the site of the musculature inside of cuticle and the muscular layer were supposed to be the major constituents of IgG inducing antigen.

Allergens↗
PubMed1985

The relation between Ames test data and electronic structure for a series of benzidine derivatives investigated by using cluster analysis.

The dose-response curves of benzidine derivatives calculated from Ames test data were analyzed by combining the least-squares method with cluster analysis. The calculations of the electronic structures of various benzidine derivatives have at the same time been performed by using the CNDO/2 method. The cluster analysis was carried out on various parameters concerning the electronic structure, such as total electron density, frontier electron density, the energy level of HOMO (highest occupied molecular orbital), and so on. It was found that the total electron density on the nitrogen atom gives a similar pattern of classification to that for mutagenic compounds in cluster analysis. In other words, it may be inferred that the nitrogen atoms of the benzidine derivatives play an important role in the metabolic activation of the compounds. Consequently, cluster analysis with a parameter of electronic structure should be a useful initial screening procedure for various analogous mutagenic compounds as well as carcinogenic compounds.

Benzidines↗
PubMed1985

[Spontaneous extradural hematoma localized in the ventral side of the thoracic spinal cord--a case report].

A case of spontaneous spinal extradural hematoma was reported. The patient, a 25-year-old housewife, was hospitalized with back pain, followed by paralysis of both legs and urinary disturbance during next 2 hours. A myelogram demonstrated that contrast medium was incompletely blocked at the level from the 3rd to the 4th thoracic spine by extradural mass. Unfortunately X-ray CT scan was not examined, however, a NMR-CT scan excluded disc protrusion and dissecting aneurysm of the aorta. Eight hours after onset, wide laminectomy was performed as emergency operation and an epidural hematoma located in the ventral side with compression of the thoracic cord was removed as completely as possible. She was discharged with excellent recovery after operation. The spontaneous spinal epidural hematoma is rare emergency. The exact etiology is unknown, and no explanation is offered as to why the hematoma is usually dorsal in location. To our knowledge, only three cases of spinal epidural hematoma which located in the ventral side were reported. Considering from our experience and other reports, it seems to be the most important point to avoid the unnecessary pressure on the spinal cord and to search the exact bleeding point in the operative procedure of ventral epidural hematomas.

Adult↗
PubMed1985

Pathologically defined neuro-, vasculo-, entero-Behçet's disease.

Behçet's disease is characterized clinically by the presence of a diagnostic triad of stomatitis, genital ulceration and uveitis. Multiple other organ systems may be involved. Prognosis is related to the presence of central nervous system, vascular or intestinal involvement. This report describes the pathology of neuro-, vasculo- and entero-Behçet's disease in a single patient.

Adult↗
PubMed1984

The N-terminal sequence of rat pepsinogen.

The amino-acid sequence of 96 residues in the N-terminal region of rat pepsinogen I was determined and the first 46 residues were found to constitute the activation peptide segment. There was high degree of homology between the activation segments of rat pepsinogen and some pepsinogens A (pig, cow, Japanese monkey and human). However, the number of residues substituted between rat and the other pepsinogens were considerably larger than those among pepsinogens A. In the N-terminal 24 residues of active pepsin, homology (88%) between rat pepsin and human gastricsin was higher than that (50%) between rat pepsin and pepsin A from human or pig. This strongly suggests that rat pepsin should be classified as pepsin C.

Amino Acid Sequence↗
PubMed1984

[Our experiences of PISCES (percutaneously inserted spinal cord electrical stimulation) in SMON and other neurologic disorders].

Percutaneously inserted spinal cord electrical stimulation (PISCES) was carried out in eleven intractable pain cases and in one spastic paraplegic case. The causes of intractable pain constitute subacute myelo-optic neuropathy (SMON) 6 cases, cerebrovascular disease 2 cases, multiple sclerosis (MS) 1 case, Charcot-Marie-Tooth (CMT) 1 case and transverse myelitis (TM) 1 case. The cause of spastic paraplegia was due to the ossification of posterior longitudinal ligament (OPLL). A trial stimulation was performed about two weeks before planning a permanent implantation of PISCES system. For the trial stimulation, epidural electrodes were percutaneously inserted with a guide of fluoroscopy in a X-ray room. The conditions of stimulation were adjusted to give an optimal electric dysesthesia. We employed pulse width 0.1-1.0 msec, pulse rate 1-120 Hz and pulse amplitude 0-10 Volt. If an excellent effect was obtained by trial study, we proceeded to the chronic implantation of PISCES system which were composed of epidural electrodes, a subcutaneous receiver and a surface antenna. The procedure of implantation was carried out in an operating room under local anesthesia. In our series, seven subjects (58%) experienced a rewarding effect by the trial stimulation and three underwent the permanent implantation of PISCES. We summarized the clinical courses of these three cases which were OPLL, CMT and SMON. Compared with the other methods for pain relief, PISCES is most characteristic in its safety and simplicity. To date, PISCES has been applied to various disorders; such as ataxia, spasticity, intractable pain, neurogenic bladder and peripheral vascular disease. But its efficacy has not been established in all these disorders.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗