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Biomedical subjects

S Takeuchi

Publications and source records attributed to S Takeuchi.

At least 685 records · Page 38Linked to original sources
PubMed1986

[Newly developed enzyme immunoassay for hCG-beta CTP and its significance in follow-up practice of trophoblastic disease].

An enzyme immunoassay (EIA) for hCG-beta CTP was newly developed. Anti hCG-beta CTP antibody was prepared from antiserum to the synthetic peptide corresponding to the carboxyl terminal region by immunization of rabbits with hCG-beta subunit. Specimens to be tested were reacted with the antibody labelled with peroxidase and determined with a spectrophotometer after the addition of o-phenylenediamine. It was found that the sensitivity of the assay for hCG in sera was 0.2miu/ml and that values for the assay were significantly correlated with those for the RIA assay for hCG-beta (r = 0.916, Y = 1.27X + 1.8). Cross reactivity with LH, FSH, hCG-beta or hCG-alpha was observed to be 0.18%, 0.05%, 10.4% or less than 0.011% respectively. The assay has been applied in the management of hydatidiform mole, invasive mole and choriocarcinoma, indicating its usefulness because of the increase in sensitivity roughly 25 times or 10 times as great as hemagglutination or hCG-beta RIA assay.

Adult↗
PubMed1986

[Effect of oncofetal antigen-I (OFA-I) on lymphocyte response in pregnant women].

OFA-I, a membrane antigen on human malignant melanoma cells, was reported to be also found on a fetal brain tissue and to have a highly immunogenic effect on a pregnant woman. The aim in this study is to analyse an immunologic activity of OFA-I on in vitro response of lymphocytes from pregnant women. OFA-I was prepared from M-14 cells which are an in vitro cell line originating in a human malignant melanoma and purified by affinity column chromatography using the antibodies which were prepared from ascites of an embryonal carcinoma patient and exhibited anti-OFA-I activity in immune adherence test. The results obtained were as follows: No significant effect of OFA-I was found on PHA-induced lymphocyte response. A suppressive effect of OFA-I was observed on an unidirectional mixed lymphocyte culture (MLC) between a wife and husband when OFA-I was added at a concentration of more than 0.1 micrograms/ml. It was also suggested that the effect was attributable to inhibition in an initial phase of MLC. A suppressive effect of OFA-I was observed on Ig production of PWM-stimulated lymphocytes at a final concentration of more than 0.25 micrograms/ml. There was no significantly difference between the effect in pregnant and non-pregnant women. Furthermore, the effect was demonstrated to be mediated with macrophages in the culture system. From these findings, it was implied that OFA-I might play an important role in maternal immune recognition in the feto-placental unit.

Adult↗
PubMed1986

[Reactivity of two monoclonal antibodies (Troma 1 and CAM 5.2) on tissue sections--as a histological trophoblast marker in normal pregnancy and trophoblastic disease].

In normal and molar pregnancy, non-villous trophoblasts which exist in the placental bed are thought to be difficult to be distinguished morphologically from the surrounding maternal cells. We examined the reactivities of two monoclonal antibodies (Troma 1 and CAM 5.2) by an immunoperoxidase technique and analyzed their usefulness as histological trophoblast markers. Materials were taken from 41 uteri of normal pregnancy, 7 uteri of hydatidiform mole, 2 uterine gestational choriocarcinoma, 1 tubal tissue of non-gestational choriocarcinoma and 5 non-gestational uteri. The reactivity of Troma 1 was observed on frozen sections and paraffin sections were employed in the analysis of CAM 5.2. The reactivities of these antibodies were the same. In nongestational tissue, they reacted with endometrial gland epithelia and endometrial covering epithelia. In placental bed of normal pregnancy, they reacted with villous and non-villous trophoblasts, besides endometrial gland epithelia. In molar sections, they reacted with gland epithelia, villous trophoblasts and also non-villous trophoblasts which proliferated towards maternal tissue. In choriocarcinoma, they reacted with carcinoma cells specifically. Since endometrial gland epithelia are easily identified histologically, it was concluded that these antibodies could work as histological trophoblast markers in normal pregnancy and trophoblastic disease.

Antibodies, Monoclonal↗
PubMed1986

[Endocrinological and clinico-pathologic study of granulosa-theca cell tumors of the ovary].

Nine granulosa-theca cell tumors (four pure theca cell tumors, one granulosa cell tumors, two granulosa-theca tumors and two juvenile granulosa-theca tumors) were studied endocrinologically and clinico-pathologically. The cases of juvenile granulosa-theca tumors developed precocious pseudopuberty. Three of seven other cases were re-feminized and four cases showed no hormonal manifestation clinically. The peripheral vein serum values of estradiol, progesterone, testosterone and prolactin were elevated in six of eight cases, three of four cases, four of six cases, and two of three cases, respectively. The concentration ratios between tumor harboring ovarian vein samples and peripheral vein (or opposite normal ovarian vein) samples was 2.7 to 16.9 for estrone, 8.8 to 28.6 for estradiol, 3.6 to 4.7 for progesterone, 1.6 to 6.6 for testosterone and 0.6 to 1.0 for prolactin. Estradiol was localized in both granulosa cells and theca cells, and testosterone was localized in granulosa cells in half of the cases and in theca cells in 60% of the cases. Also, testosterone was localized in all three cases in which luteinized theca cells were present. There were no cases with positive prolactin localization. These results are compatible with the concept that in granulosa-theca cell tumor, both granulosa and theca cells can produce a wide range of steroid hormones.

Aged↗
PubMed1986

[Clinical studies on the effect of imipenem/cilastatin sodium on infections in obstetrics and gynecology. Tissue concentrations and clinical effects].

Tissue transfer and clinical effects of imipenem/cilastatin sodium (MK-0787/MK-0791), a new carbapenem antibiotic, were studied and the following results were obtained. Penetrations of MK-0787 into uterine arterial blood and into pelvic dead space exudate were good. When MK-0787/MK-0791 was administered at a dose of 500 mg/500 mg by a 30-minute intravenous drip infusion, the peak level of MK-0787 in uterine arterial blood was 22.2 micrograms/ml, 30 minutes after the completion of the drip infusion. The peak level of MK-0787 in pelvic dead space exudate was 12.9 micrograms/ml at 2 hours and it dropped to 2.6 micrograms/ml at 6 hours. MK-0791 levels were similar to those of MK-0787. Penetrations of MK-0787 into tissues were also good. When MK-0787/MK-0791 was administered at a dose of 500 mg/500 mg by a 30-minute intravenous drip infusion, the level of MK-0787 was 2.2 +/- 1.1 micrograms/g in the oviduct, 2.7 +/- 2.1 micrograms/g in the ovary, 2.5 +/- 1.2 micrograms/g in the endometrium, 3.0 +/- 1.6 micrograms/g in the myometrium, 3.1 +/- 1.9 micrograms/g in the cervix uteri and 3.8 +/- 2.0 micrograms/g in the portio vaginalis at 1 hour after administration. These levels were reduced to halves, respectively, in approximately 2 hours. Four patients with intrauterine infections and 2 with vaginal stump infections were treated with MK-0787/MK-0791 at a daily dose of 1 g/1 g (500 mg/500 mg X 2). Good clinical and bacteriological responses were observed in 5 patients and causative organisms were eradicated in 2 patients.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1986

[Immunohistologic characterization of lymphoid cells infiltrating dysgerminoma of the ovary].

Dysgerminoma of the ovary is composed of cells that resemble primordial germ cells morphologically and connective tissue stroma with lymphoid cell infiltration, the degree of which has been discussed in correlation with the prognosis. In order to characterize the lymphoid cells histochemically, a sensitive immunoperoxidase technique was applied on serial tumor tissue sections. Monoclonal antibodies used in this study were anti-HLA-ABC, anti-HLA-DR, anti-HLe-1 (leukocytes), anti-Leu-1,-4(pan T cells), anti-Leu-2a (killer/suppressor T cells), anti-Leu-3a (helper/inducer T cells), anti-Leu-12,-14 (B cells), anti-Leu-M3 (monocytes/macrophages) and OKT-10 (myeloid and lymphoid precursors, thymocytes, activated T and B cells). Lymphoid cells identified morphologically on sections stained with hematoxylin-eosin were HLe-1 positive, and were therefore thought to originate in bone marrow. Most of them were also positive for Leu-1,-4, and they were composed of nearly equal numbers of Leu-2a positive cells and Leu-3a positive cells. Furthermore, the majority of the lymphoid cells were HLA-DR positive, consisting of a large number of OKT-10 positive cells and a small number of Leu-12,-14 positive cells and Leu-M3 positive cells which were mainly found in connective stroma encapsulating tumor tissue. Taken together, the lymphoid cells in the connective stroma were considered to be activated T cells. Tumor cells themselves lacked reactivity with anti-HLA-ABC or anti-HLA-DR, while lymphoid cells and connective stroma were HLA-ABC positive.

Dysgerminoma↗
PubMed1986

[Pathological and endocrinological study of epithelial ovarian tumors in post-menopausal women].

We have studied 37 cases of ovarian epithelial tumors in post-menopausal women, histopathologically and endocrinologically. The normal values for androstenedione, estrone, estradiol and progesterone in healthy post-menopausal women were less than 83 ng/dl, 75 pg/ml, 30 pg/ml and 0.6 ng/ml, respectively. The numbers of cases, the serum values for which were higher than normal, were 9 of 16 (androstenedione), 9 of 16 (estrone), 22 of 29 (estradiol) and 14 of 20 (progesterone). The serum levels of these 4 sex hormones in cases with ovarian stromal condensation (stromal cell hyperplasia) were higher than normal in 8 of 11 (androstenedione), 8 of 11 (estrone), 17 of 19 (estradiol) and 9 of 12 (progesterone), whereas those in cases with no stromal condensation were elevated in 1 of 5, 1 of 5, 5 of 8 and 5 of 7, respectively. After complete removal of the tumors, these elevated sex hormone levels dropped to normal. After the dexamethasone suppression test, the suppression rates for cortisol, 17-OHCS and 17-KS were 4 times as great as those of DHEA-S (dehydroepiandrosterone-sulfate), estrone, estradiol and progesterone. 17 beta-estradiol was localized in hyperplastic ovarian stromal cells in all cases with stromal condensation. We concluded that many of the ovarian epithelial tumors produce these sex hormones and that hyperplastic stromal cells are the source an increased amount of serum estradiol.

Adenocarcinoma↗
PubMed1986

[Characteristics of heterologous membrane antigen (HM Ag) and humoral immune response to HM Ag in pregnant women and patients with gynecologic malignancies].

Heterologous membrane antigen (HM Ag) is discovered to be incorporated into membranes of cultured human cells grown in media containing fetal calf serum (FCS). To know the characteristics of HM Ag, we investigated the dynamics of HM Ag on cell membrane, distribution of HM Ag and humoral immune response to HM Ag in pregnancy and gynecologic malignancies. The following results were obtained. HM Ag disappeared from the cell membranes of M14 grown in media with fetal calf serum (FCS) after the cells were transferred into whole human serum (12 days) or into a gamma human serum (18 days). M14 cells grown in human serum reincoporated HM Ag into their membranes within 48 hours after transfer to medium with 20% FCS. HM Ag was detected in FCS, adult bovine serum, cow's milk and erythrocytes homogenate of ox and sheep. The natural anti-HM Ag antibody appeared frequently in pregnant women, patients with gynecologic malignancies and healthy persons. These findings suggest that HM Ag must be excluded in immunologic studies of cultured human cancer cells by changing the medium from FCS to human serum or by absorbing anti-HM Ag antibody with cells carrying HM Ag.

Antigens, Surface↗
PubMed1986

[Anti-tumor and adverse effect of etoposide and cisplatin on human choriocarcinoma transplanted to nude mice--a correlation between effect and tissue distribution of the drugs].

The study dealt with anti-tumor and adverse effects of etoposide and cisplatin on human choriocarcinoma cell line (GCH-1) transplantable in nude mice in relation to the rate of their uptake into tumor tissue. Nude mice were divided into 3 groups (etoposide, cisplatin and MTX-group), 7 to 9 per group and received drug treatment which started when their tumor grew to 100 approximately 300 mm3 in volume. All drugs were intraperitoneally administrated to nude mice (each drug: 1/4 mouse LD50 dose X3, weekly). The inhibiting action on the tumor was observed to be etoposide greater than cisplatin greater than MTX. The body weight loss of nude mice was observed to at its maximum in the cisplatin-treated group. The serum beta-HCG level did not rise in the drug-treated groups but rose in the non-treated control group. No histopathological changes characteristic of each drug were found. A concentration of etoposide and cisplatin in various tissues was serially measured after one shot intraperitoneal injection of etoposide 25 mg/kg or cisplatin 5 mg/kg. Etoposide reached its peak concentration after 30 minutes in tumor, liver and kidney and, after 4 hours, was left with 37.1%, 10.4% and 2.3% concentrations after 30 minutes in tumor, liver and kidney, respectively. Cisplatin showed similar kinetics, but was found to remain at a comparatively high concentration in both liver and kidney. Thus, it was demonstrated that etoposide and cisplatin were active against the human choriocarcinoma cell line. Furthermore, the less adverse effect of etoposide was felt to be partially due to its lower residue in liver and kidney.

Animals↗