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Biomedical subjects

S Takeuchi

Publications and source records attributed to S Takeuchi.

At least 649 records · Page 36Linked to original sources
PubMed1987

The effect of glycemic control on plasma soluble fibrin monomer complexes and fibronectin in diabetic patients.

We have shown previously that increased concentrations of plasma soluble fibrin monomer complexes (SFMC) and elevated fibronectin (Fn) levels are closely related to the development of diabetic microangiopathy. The purpose of the present study was to explore whether or not changes in plasma glucose levels could have an effect on these protein constituents. Plasma glucose levels of 25 uncontrolled diabetic patients were brought under control with insulin and serial measurements of SFMC and Fn were made over a period of 4 weeks. Glucose values fell from an average of 312 mg/100 ml to 160 mg/100 ml. Ten patients with macroproteinuria (i.e. greater than or equal to 0.5 g/24 hr) showed initially elevated plasma SFMC and Fn concentrations. These levels fell significantly over the 4 week observation period: from 13.6 mg/100 ml to 9.4 mg/100 ml for SFMC and from 38.4 mg/100 ml to 34.5 mg/100 ml for Fn. The remaining 15 patients had nearly normal levels of both SFMC (7.9 mg/100 ml) and Fn (31.1 mg/100 ml) and glycemic control brought no further reduction. The data indicated that a) elevated SFMC and Fn levels are indeed associated with diabetic microangiopathy, especially in the presence of macroproteinuria; and b) adequate glycemic control is capable of normalizing the plasma concentration of these constituents.

Adult↗
PubMed1987

Immunohistochemical localization of placental proteins and tumor-associated antigens in endometrial cancer and endometrial hyperplasia.

As to endometrial cancer and endometrial hyperplasia, this study represents the localizations of the placental proteins and the tumor-associated antigens by both immunohistochemical light microscopy (ILM) and immunoelectron microscopy (IEM). The reagents examined include human placental lactogen (HPL), placental alkaline phosphatase (PAP), pregnancy-associated plasma protein A (PAPP-A), pregnancy-specific beta 1-glycoprotein (SP1), alpha 1-anti-trypsin (alpha-AT), and tissue polypeptide antigen (TPA). The tumor cells stained for HPL are localized and the SP1-positive cancer cells are almost entirely restricted to an infiltrating front. alpha-AT is shown in both tumor cells with marked cellular atypism and ones in deeply infiltrating foci. The immunoreactive frequency for the above six reagents in endometrial cancer is higher than that in endometrial hyperplasia. In endometrial cancer, the high frequency of an immunoreaction is confirmed in TPA (p less than 0.05). The combined stainings, i.e. either for TPA and SP1 or for TPA and PAPP-A, are efficient immunohistochemical screening methods to use in diagnosing endometrial cancer in the biopsied specimens (p less than 0.05).

Adenoma↗
PubMed1987

Breast self-examination practice and clinical stage of breast cancer.

The relationship between breast self-examination (BSE) and pre-treatment clinical stage of breast cancer was assessed in female breast cancer patients consisting of 30 patients practicing BSE monthly, 60 patients practicing occasionally, and 60 patients who had rarely or never practiced BSE. These patients were matched by age, residence, and hospital. More frequent practice of BSE was associated with more favorable clinical stage. The percentage of stage I patients was 33% for monthly performers, and this was significantly higher than the value of 14% for those who had rarely or never practiced. BSE practice was significantly related to tumor size measured before treatment in a dose-response manner. The average values of maximum tumor diameter were 2.5 cm for monthly performers, 3.0 cm for occasional performers, and 3.5 cm for those who had rarely or never practiced. The results suggest that BSE practice increases the probability of detecting smaller cancers at an early stage, and this may lead to a more favorable prognosis.

Adult↗
PubMed1987

[Immunohistochemical localization of hCG alpha, hCG beta CTP, hPL and SP1 on villous and extravillous trophoblasts in normal human pregnancy].

Trophoblasts are divided into villous trophoblasts and extravillous trophoblasts, depending on whether they constitute a villous structure, and cell columns intervene between them. We conducted an immunohistochemical localization of human chorionic gonadotropin (hCG) alpha, hCG beta C-terminal peptide (CTP), human placental lactogen (hPL) and pregnancy-specific beta 1-glycoprotein (SP1) on the trophoblasts of normal human pregnancy, using forty-one hysterectomized pregnant uteri (6-22 weeks). In villous trophoblasts, the capacity to synthesize hCG alpha, hCG beta CTP, hPL and SP1 seemed to develop according to the morphological change from mononuclear cells to multinuclear cells. In contrast, the synthetic capacity of these proteins seemed not to correspond with the morphological change in extravillous trophoblasts: The location of hCG alpha, hCG beta CTP and SP1 was restricted to the mononuclear trophoblasts in the superficial decidua, while hPL was present extensively in extravillous trophoblasts, including multinuclear trophoblasts in the deciduomuscular junction. Therefore, it may be reasonably said that extravillous trophoblasts have many biological features distinct from villous trophoblasts and differentiate in an independent manner. Mononuclear trophoblasts in the cell column were negative for these proteins, which, together with morphological observations, strongly suggest the germinative nature of these cells.

C-Peptide↗
PubMed1987

[Plasma fibronectin level in patients with moyamoya disease].

Moyamoya disease is generally considered as a slowly progressive occlusive disease of the circle of Willis. Pathological investigation has revealed that the occlusion develops due to fibrocellular thickening of the intima. In spite of various studies, however, the etiology of this disease and the mechanism of the intimal thickening remain unknown. Fibronectin (FN) is a high molecular weight glycoprotein which is found in an insoluble form in many tissues and in a soluble form in plasma and other body fluids. FNs have many biological activities and play an important role in the formation of connective tissue under physiological and pathological conditions. In the present study we have determined the plasma FN levels in order to investigate the relationship between FN and moyamoya disease. The subjects were 39 patients with moyamoya disease who were in the chronic stage after reconstructive surgery, 39 age- and sex-matched normal healthy controls, and 39 patients with atherosclerotic cerebrovascular disease in the chronic stage. The plasma FN levels were measured by the single radial immunodiffusion method using venous blood samples. The plasma FN level in healthy subjects was 33.9 +/- 6.3 mg/dl (mean +/- standard deviation) which almost agreed with the level, 30-35 mg/dl, reported by some investigators. In the patients with moyamoya disease the level was 42.5 +/- 7.9 mg/dl, showing a significant increase (p less than 0.001) compared with the healthy subjects. Then the subjects were classified into a child group (15 years or younger) and an adult group (16 years or older) and their levels were examined.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1987

[Morphological changes in the contralateral testis in patients with testicular tumor].

Biopsy of contralateral testis of 20 patients with an unilateral testicular tumor was carried out to evaluate the morphological changes of the contralateral testis caused by the tumor. Serum beta-chorionic gonadotropin (beta-hCG), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and alpha-fetoprotein (AFP) levels before orchiectomy were examined as a function influencing morphological changes of the contralateral testis. Typical carcinoma in situ was found in a case of ipsilateral testis. The presence of a few atypical germ cells, however, was the only finding of the contralateral testis of three patients. Five of 20 patients revealed hypospermatogenesis in the contralateral intact testis. Most of the other patients also showed sloughing of germinal epithelium suggesting disorder of spermatogenesis. Johnsen's mean score count and tubular wall thickening were related to neither clinical stages, ESR, serum beta-hCG, AFP, LH nor FSH levels. Tubular wall thickening was observed in 8 of 10 patients with a tumor weighting over 150 g, but was found in only 1 of 10 patients with a lighter tumor (p less than 0.01).

Adult↗
PubMed1987

[Clinico-pathological study of choriocarcinoma localized in the lung with emphasis on hCG monitoring systems].

We studied 15 cases of lung choriocarcinoma clinico-pathologically with emphasis on hCG monitoring systems. The results are as follows: Vivid tumor cells (VTC) were found to persist in 83.3% (5/6) of cases with a continued "LH level" of urinary hCG over 4 weeks. VTC were found to persist in all 4 cases with cellular response and in 6 of 8 cases with no cellular response. The syncytial cell element was observed in 75% (3/4) of cases with cellular response, but in only 25% (2/8) of cases with no cellular response. VTC were recognized in 83.3% of cases (5/6) with serum beta-hCG higher than the sensitivity of the beta-hCG-RIA system (0.2 ng/ml or 1.5 miu/ml). Even though serum beta-hCG was lower than 0.2 ng/ml or 1.5 miu/ml, VTC were found to persist in 2 of 3 cases. All of 5 cases with beta-hCG-CTP higher than the sensitivity of beta-hCG-CTP-EIA (Enzyme-Immuno-Assay) system (0.2 miu/ml) showed VTC in operative specimens. These results indicate that beta-hCG-CTP-EIA is a most useful means to employ in the monitoring of serum hCG.

Choriocarcinoma↗
PubMed1987

[Regional registration study of uterine neoplasia in Niigata Prefecture].

In this study, we examined the incidence of the uterine neoplasia in Niigata prefecture on the basis of the data collected by the Niigata Gynecologic Cancer Registry between 1982 and 1984. The results are as follows: The registered cases with dysplasia (Dysp.), cervical carcinoma in situ (CIS), cervical invasive carcinoma (Inv. Ca.) and endometrial carcinoma (End. Ca) were 358, 147, 530 and 141, respectively. One hundred and ninety-six cases with Dysp., 81 cases with CIS, 99 cases with Inv. Ca. and 10 cases with End. Ca. were detected by mass cancer screening. The crude incidence rate for Dysp., CIS, Inv. Ca. and End. Ca. was 9.44, 3.88, 13.98 and 3.72, respectively. The age standardized incidence rate (all age, world population) for Dysp., CIS, Inv. Ca. and End. Ca. was 7.48, 3.08, 9.45 and 2.63, respectively. The age standardized incidence rate (over 30 years, world population) for Dysp., CIS, Inv. Ca, and End. Ca. was 16.48, 6.77, 21.00 and 5.90, respectively. The lifetime incidence rate for uterine carcinoma in women aged 0 approximately 75 years was 1.65%.

Adult↗
PubMed1987

[Phase II study of cisplatin in cervical and endometrial carcinomas].

A phase II group study of cisplatin for cervical and endometrial carcinomas was carried out in 19 institutes throughout Japan. The patients entered consisted of 62 women with cervical and 7 with endometrial carcinoma of whom 39 and 4 were evaluable, respectively. Cisplatin was administered in either of two regimens; 10-20 mg/m2 i.v., on days 1-5, or 50-100 mg/m2 i.v., on day 1, every 3 to 4 weeks. The responders comprised 4 CRs and 10 PRs for cervical carcinoma and 1 CR and 2 PRs for endometrial carcinoma, and the response rates were 35.9% and 75.0%, respectively. The response rates by histological classification were 39.4% (13/33) for squamous cell carcinoma and 16.7% (1/6) for non-squamous cell carcinoma. Response rates analysed by lesion site were 33.3% for primary tumors, 36.8% for local lesions and 33.3% for metastases. Furthermore, the response rate among patients without any prior chemotherapy was 44.4% vs. 16.7% for those with prior chemotherapy. Adverse effects included nausea and vomiting (95.3%), anorexia (93%), anemia (72.1%), leucopenia (60.5%) and elevation of BUN (16.3%). Adverse effects were tolerable. We concluded from these results that cisplatin is among the most efficacious and useful drugs against cervical (and endometrial) carcinoma(s).

Adenocarcinoma↗
PubMed1987

[Clinico-pathological study of the partial hydatidiform mole].

We studied 302 spontaneous abortuses with no molar swelling of villi (S.Ab.) and 156 partial moles (P.M.), clinico-pathologically. The results were as follow: The estimated "in utero retention time" of the conceptus of S.Ab. and P.M. was 24.8 +/- 13.1 days (n = 63) and 45.9 +/- 10.7 days (n = 37), respectively (p less than 0.01). Among the cases with "in utero retention time" of 4 or more weeks, 9 of 35 S.Ab. and 32 of 37 P.M. showed no degenerative change of trophoblasts. IgG was localized on the cell membrane of the syncytiotrophoblasts in 19 of 63 S.Ab. and 27 of 37 P.M. (p less than 0.01). IgM was also localized on the cell membrane of syncytiotrophoblasts in 29 of 63 S.Ab and 6 of 37 P.M. (p less than 0.01). Six patients (3.85%, 6/156) with P.M. received operative procedure and/or chemotherapy after evacuation of the mole. Four had invasive mole, on had metastatic lesion in the lung and one had persistent trophoblastic disease. Four of these six patients with subsequent disease were 40 years of age or older. All patients with partial mole needed hCG assay monitoring because of the risk of abnormal sequelae.

Female↗
PubMed1987

[Clinico-pathologic study on the early diagnosis of cervical adenocarcinoma].

We studied 94 cases of adenocarcinoma of the uterine cervix, with emphasis on early diagnosis of cervical adenocarcinoma, histopathologically. The results are as follows: The number of cases was 4 in Stage 0 (adenocarcinoma in situ, ACIS), 51 in Stage I, and 39 in Stage II. The proportion of ACIS to invasive adenocarcinoma was 4 of 94 (4.26%) and was significantly lower than that of cervical squamous cell carcinoma. Thirty-nine cervical biopsies of 29 cases, taken 1-3 years prior to the clinical presentation of cancer, were available for study. In 2 of 16 "negative specimens", areas of glandular dysplasia were found on review. Areas of ACIS and adenocarcinoma with early stromal invasion were also found on review in 5 and in 2 of 14 specimens diagnosed as atypical glands, respectively. This study strongly suggests that "atypical glands" such as glandular dysplasia and ACIS are precursor lesions of invasive adenocarcinoma of the uterine cervix and that the delay in diagnosing cervical adenocarcinoma may be due partly to "underdiagnosis" of cervical biopsy specimens.

Adenocarcinoma↗