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Biomedical subjects

S Subramanian

Publications and source records attributed to S Subramanian.

At least 217 records · Page 12Linked to original sources
PubMed1985

Long-term results after excision of fixed subaortic stenosis.

Forty-nine patients underwent surgical excision of fixed subaortic stenosis (discrete fibrous ring and tunnel) between 1968 and 1984 and were followed up for 1 to 16 years (5.8 +/- 4). Twenty-six patients (Group I) had isolated subaortic stenosis and 23 (Group II) had subaortic stenosis and associated cardiac defects. Discrete fibrous ring was present in 46 and tunnel type of obstruction in three patients. For the discrete ring, excision alone was done in 32 patients (four recurrences) and excision with myotomy in 17 (three recurrences). In Group I, there were no operative deaths and one late death from a noncardiac cause. In Group II, one early and two late deaths occurred. The actuarial survival rate for 10 years is 88%. Reoperations were performed in 10 patients, seven for recurrence and three for aortic valve replacement for preexisting aortic regurgitation that had progressed since the primary operation. Cardioplegia was used in 28 patients (one recurrence) and was not used in 21 patients (six recurrences). An operative residual gradient of less than 15 mm Hg was achieved in 25 of 28 patients in whom cardioplegia was used compared to 12 of 21 patients in whom cardioplegia was not used (p = 0.017). These results indicate that complete excision of the ring with the aid of cardioplegia has significantly reduced the recurrence rate of subaortic stenosis (p = 0.033), elimination of the residual gradient at the initial operation has been a significant factor in reducing the recurrence rate (p = 0.017), and addition of myotomy in this series has not altered the outcome.

Adolescent↗
PubMed1984

Evidence for repetitive structure of the large acyl-carrier protein subunit of Escherichia coli citrate lyase.

The amino acid composition of the unusually large acyl-carrier protein subunit of citrate lyase from Escherichia coli is characteristic of a protein with a highly repetitive structure. Peptide mapping studies provide further evidence of repetitive sequences within the subunit. Only a single Pauly-positive spot is detected in the tryptic peptide map although the subunit contains 8 histidine residues. The 4 prosthetic groups covalently bound to the subunit are recovered in a single tryptic fragment in almost quantitative yield. These structural features of the large acyl-carrier protein subunit probably reflect internal gene duplications.

ATP Citrate (pro-S)-Lyase↗
PubMed1984

Humoral and cell-mediated immune responses to leptospires in different human cases.

Humoral and cell-mediated immune responses (CMI) to leptospirosis were studied in different groups of human patients. These included clinically suspected cases of leptospirosis, patients with jaundice (HBsAg negative), cases of pyrexia of unknown origin (PUC) (Widal and blood culture negative for typhoid) and healthy blood donors. Humoral immune response was evaluated with nine live strains representing different serogroups of leptospires and CMI with lysates of four strains. The study showed that the CMI appeared earlier than the humoral response which might be useful in the early detection of the disease. As in the humoral immune response, cross reactions to different leptospires were also seen in CMI response.

Antibody Formation↗
PubMed1984

Mycotic aortic aneurysms in children.

Mycotic aneurysms of the aorta are uncommon in babies and children. Prior to the development of antibiotics, most mycotic aneurysms were seen secondary to bacterial endocarditis, but this is now uncommon. Instead, more cases have been reported as complications of umbilical artery catheters in newborns. We have seen five cases of mycotic aneurysms in children, two of them secondary to umbilical artery catheters. One patient had coarctation of the aorta, and the other patients had different sources of infection. Three patients were treated surgically by us with good results after antibiotic therapy. One patient died of sepsis before the aneurysm was diagnosed. The fifth patient was treated elsewhere and now has a recurrent aneurysm. We think a combination of aggressive medical and early surgical therapy may save a high percentage of these patients.

Aneurysm, Infected↗
PubMed1984

Congenital diverticulum of the heart of biventricular origin.

An extremely rare diverticulum arising from both ventricles at the caudal end of the intraventricular septum was diagnosed in a 2.9 kg infant. The diverticulum was removed successfully while a coexisting ventricular septal defect was left open for further management. The types and complications of such diverticula are described and the need for early surgical intervention is stressed.

Diverticulum↗
PubMed1984

Kinetics of peripheral blood T cell numbers and functions in patients with burns.

Cell-mediated immunity (CMI) was assessed in 58 patients with burns involving 10% to 40% of the total body surface area (TBSA), and in 16 normal healthy individuals, by using two parameters, the total T-cell counts in peripheral circulation and T-cell function as indicated by the leukocyte migration inhibition (LMI) test. Three recall antigens from Candida albicans, S. typhi, and E. histolytica were used to elicit the LMI. T-cell function was found to be significantly depressed in burns involving more than 25% TBSA. Depression of T-cell function started after the seventh postburn (PB) day and continued until the sixtieth PB day.

Antigens↗
PubMed1984

Dye-ligand affinity chromatography: the interaction of Cibacron Blue F3GA with proteins and enzymes.

The dye Cibacron Blue F3GA has a high affinity for many proteins and enzymes. It has therefore been attached to various solid supports such as Sephadex, Sepharose, polyacrylamide, and the like. In the immobilized form the dye has rapidly been exploited as an affinity chromatographic medium to separate and purify a variety of proteins including dehydrogenases, kinases, serum albumin, interferons, several plasma proteins, and a host of other proteins. Such a diversity shown by the blue dye in binding several unrelated classes of proteins has generated considerable work in terms of studies of the chromophore itself and also the immobilized ligand. As a prelude to realizing the full potential of the immobilized Cibacron Blue F3GA, an understanding of the basic interactions of the dye with its surroundings must be gained. It has been recognized that the dye is capable of hydrophobic and/or electrostatic interactions at the instance of the ambient conditions. The study of interactions of the dye with salts, solvents, and other small molecules indicates the nature of the interactions of the dye with different kinds of groups at the interacting sites of proteins. The review will cover such interactions of the dye with the proteins, the interactions of the proteins with the immobilized ligand, and the media used to elute the bound protein in several cases, and thus consolidate the available information on such studies into a cogent and comprehensive explanation.

Blood Proteins↗
PubMed1984

Aortoplasty and aortic valve replacement for annuloaortic ectasia in an infant with Marfan's syndrome, forme fruste.

This report describes our experience with a 4-month-old infant with annuloaortic ectasia in whom intractable heart failure developed because of acute exacerbation of aortic regurgitation. He underwent an aortoplasty with aortic valve replacement and made an uneventful recovery. Since there has been no report of a successful repair of this condition in infancy, the case is presented and relevant problems are discussed.

Aorta↗
PubMed1984

Total bloodless open heart surgery in the pediatric age group.

Forty-eight pediatric open heart surgical procedures were performed with bloodless techniques regardless of surgical complexity or presence of cyanosis at the Children's Hospital of Buffalo. Priming solution for cardiopulmonary bypass was reduced to avoid excessive hemodilution, and careful surgical techniques were used to minimize blood loss. Hypothermia compensated for decreased oxygen-carrying capacity and made it possible to reduce bypass flow safely. Tissue perfusion and oxygenation on bypass appeared comparable to or better than those in a control group that underwent open heart surgery with milder degrees of hemodilution. All patients tolerated bypass uneventfully without transfusion. Platelet counts were higher and blood loss less marked than those in the control group. Prevention of excessive dilution by priming solution seemed most important for preservation of platelets and subsequent hemostasis. Four of the smaller infants with complex cyanotic defects needed postoperative transfusion, while 44 patients did not receive transfusion at all during their hospital stay. Total blood product requirement was reduced from 11.5 to 0.35 units per patient. Intracardiac surgery without transfusion is possible in most pediatric patients without evidence of increased risk.

Blood Transfusion↗
PubMed1983

S-acylated residues of the acyl-carrier protein subunit of Klebsiella aerogenes citrate lyase.

Oxidation of the isolated deacetyl acyl-carrier protein subunit of citrate lyase from Klebsiella aerogenes with Cu2+-o-phenanthroline complex leads exclusively to intrapeptide disulfide bridge formation indicating that the cysteamine and the cysteine residues are located in close proximity. The S-acetylation of the cysteine residue in deacetyl acyl-carrier protein subunit is catalysed by a citrate lyase ligase preparation in presence of acetate and ATP. Reaction-inactivation of citrate lyase results in deacetylation of the S-acetyl cysteamine residue of the prosthetic group but not of the S-acylated cysteine residue in the acyl-carrier protein.

Acyl Carrier Protein↗
PubMed1983

The presence of essential arginine residues at the active sites of citrate lyase complex from Klebsiella aerogenes.

The acyl-transferase and acyl-lyase activities of Klebsiella aerogenes citrate lyase complex are inactivated by the arginine specific reagents phenylglyoxal and 2,3-butanedione, the former reagent being the more potent inhibitor. Citrate and (3S)-citryl-CoA protect the transferase activity, while acetyl-CoA markedly enhances the rate of the inactivation. (3S)-Citryl-CoA protects the lyase subunit in the complex from inactivation. The kinetics of inactivation suggest the involvement of a single arginine residue at each of the active sites of the transferase and of the lyase subunits.

Acyl Coenzyme A↗
PubMed1983

Serological evidence of leptospirosis in a human population following an outbreak of the disease in cattle.

A serological study of leptospirosis in a human population, mainly children of a village near Madras City in Tamilnadu State, India, was undertaken following an outbreak of the disease in cattle as evidenced by clinical symptoms and serology. 27 (68%) of the 40 animal sera tested were positive for leptospiral antibodies. Though cross reactions were present, higher titres and a greater number of positive reactions were seen against autumnalis antigen. 35 (47%) of the 75 human sera gave positive antibody titres and, of these, 22 showed the presence of antibodies to autumnalis antigen.

Adolescent↗