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Biomedical subjects

S Silverman

Publications and source records attributed to S Silverman.

At least 145 records · Page 8Linked to original sources
PubMed1987

AIDS update: oral findings, diagnosis, and precautions.

The dimensions of AIDS continue to increase. The number of people infected with the AIDS virus (HIV) grows and no effective vaccine or curative treatment is expected in the near future. This article describes the oral findings of AIDS and AIDS-related complex (ARC). It will help dentists in recognition, diagnosis, consultation, and office precautions. These oral findings are important considerations in treatment planning and staging for patients with AIDS and ARC.

AIDS-Related Complex↗
PubMed1987

Isolation of a human brain cDNA for glutamate dehydrogenase.

A cDNA has been isolated from a human brain expression library using anti-bovine glutamate dehydrogenase (GDH) antibodies. The cDNA has an open reading frame of 774 nucleotides, which codes for 258 amino acids. The 258-amino-acid sequence is 95% homologous to the carboxy terminus of human liver GDH. This high degree of homology indicates that the cDNA codes for brain GDH. Fourteen differences between the amino acid sequence deduced from this cDNA and the sequence reported for human liver GDH suggest that there may be two active human GDH genes. A cRNA probe synthesized from the cDNA detects a 3.7-kilobase (kb) mRNA from human brain. Rat liver and kidney each contain two GDH mRNAs, 3.5 and 2.8 kb, respectively. The 3.5-kb transcript is prominent in rat brain, whereas the 2.8-kb transcript is barely detectable, a result suggesting that GDH gene expression is differentially controlled in rat brain.

Amino Acid Sequence↗
PubMed1987

A monoclonal antibody to squamous cell carcinoma.

Monoclonal antibody 17.13.Cl.10 is a murine IgM kappa monoclonal antibody (Mab) that stains frozen section squamous cell carcinoma (SCC) homogeneously and intensely with a sensitivity greater than 98%, including 106/107 SCC specimens from the head and neck. It was produced using a human laryngeal SCC as immunogen and screened using frozen section human tissue. Monoclonal antibody 17.13.Cl.10 faintly stains the basal layer of normal squamous epithelium, does not stain normal organ tissue other than myoepithelial cells, and reacts with few non-SCC tumors. It, therefore, may be a useful adjunct to standard histopathologic criteria for the diagnosis of SCC. It may prove helpful in the investigation of tumor-associated antigens. Despite major technical and immunologic problems, monoclonal antibodies to functional tissue-specific tumor-associated antigens have the potential to play a major role in imaging and in treatment in the future.

Animals↗
PubMed1987

Penetration of bacteria and spermatozoa into bovine cervical mucus.

It has been reported that bacteria may attach to motile spermatozoa, be carried through the cervix and uterus to the fallopian tubes, and cause acute salpingitis. In an attempt to mimic these conditions in vitro, we incubated Escherichia coli, Streptococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus with motile spermatozoa which then were allowed to migrate through a capillary filled with bovine cervical mucus. After satisfactory sperm migration through the mucus, the capillaries were broken and cultured at different distances from the original insemination site. Mucus fractions in proximity to bacterial inocula grew varying amounts of the pathogens. More distal fractions of mucus columns were generally culture-negative even though they contained motile sperm which had been exposed to bacteria. Migration of spermatozoa exposed to bacteria through bovine cervical mucus did not result in enhanced bacterial penetration.

Animals↗
PubMed1987

Prevention, early detection and diagnosis of oral cancer.

Oral cancer persistently accounts for approximately 3 to 4 per cent of all newly diagnosed cancers each year in the United States, and absolute numbers increase yearly. Morbidity and mortality can be minimized by a combination of early diagnosis and appropriate treatment. Early diagnosis can be aided by application of vital staining with toluidine blue or use of exfoliative cytology combined with adequate tissue biopsy. Prevention--minimizing or avoiding the use of tobacco and alcohol--can play a significant role in oral cancer control, as can proper management of precancerous lesions.

Adolescent↗
PubMed1987

Infectious disease control and the dental office: AIDS and other transmissible diseases.

In spite of attempts to modify homosexual behaviour and the sharing of contaminated needles by drug abusers, AIDS virus infections continue to spread throughout the world. The potential for transmission, the lack of a vaccine and effective treatment, and the lethal nature of the disease cause great alarm and influence health care delivery. Dentistry is involved because oral health care is being given knowingly or unknowingly to high-risk individuals. These high-risk individuals are the sources for many other infections with viral, bacterial, fungal and protozoal organisms. In spite of the fact that spread does not occur through casual contact, concern is justified since many questions involving transmissibility and susceptibility have not been completely resolved. Therefore, dental professionals must follow infectious disease control guidelines to protect themselves as well as other patients. Because oral manifestations of those infected by the AIDS virus are common, dental professionals also play a role in recognition, counselling and referral.

Acquired Immunodeficiency Syndrome↗
PubMed1986

Five-year survival of patients with oral cancer and its association with antibody to herpes simplex virus.

Levels of antibody to herpes simplex virus type 1 (HSV-1) were measured in 70 patients with untreated squamous cell carcinoma of the mouth. After treatment the actuarial survival was determined at quarterly intervals for 5 years and was found to be associated with the pretreatment level of antibody to the virus. Patients with levels of IgM antibody to HSV-1 which were above the median level had a 5-year survival of only 56% whereas those with levels below the median had a higher survival of 72%. Patients with no detectable IgM antibody to HSV-1 had a 5-year survival of 81%. The reverse was seen with IgG antibody to HSV-1. Patients with higher than the median level of IgG antibody had a 5-year survival of 73%, whereas those with IgG antibody below the median had a 5-year survival of 56%. No relationship was seen between survival and levels of IgA antibody to HSV-1, or between survival and antibody of any class to cytomegalovirus. The data are consistent with the reported association between oral cancer and HSV-1.

Aged↗
PubMed1986

Oral mucous membrane pemphigoid. A study of sixty-five patients.

Sixty-five patients with oral mucous membrane pemphigoid were examined, treated, and followed for a mean of 3.5 years. Seventy-two percent were women, and the mean age was 59 years. In most patients multiple oral sites were involved and the gingiva was affected in 94% of the patients. Seven patients had ocular lesions (symblepharon), and seven others had nasal, vaginal, or pharyngeal involvement. Persons with skin lesions (bullous form) were not included in this series. Topical and systemic anti-inflammatory/immunosuppressive medications were successful in controlling signs and symptoms. There were no spontaneous remissions. Candidiasis, tobacco, other diseases, and drugs did not appear to be related to the pemphigoid.

Adult↗
PubMed1986

Malignancy grading in oral squamous cell carcinoma. I. Squamous cell carcinoma of the tongue and floor of mouth: histologic grading in the clinical evaluation.

In a retrospective histological and clinical study, a multifactorial grading system was used for histological classification and grading of malignancy in 51 cases of squamous cell carcinoma of the tongue and floor of mouth. The results indicated that the use of such a multifactorial grading system, consisting of 7 morphological parameters, including an evaluation of both the tumor cell population itself and the tumor-host relationship, can serve as an important supplement to clinical judgement in determining the outcome of the oral tumor.

Adult↗
PubMed1986

Phenotypic identification of mononuclear cells in oral premalignant lesions and cancer by monoclonal antibodies.

To explore the nature and importance of mononuclear cells of different phenotypes in oral premalignant lesions and oral cancer, we studied biopsy specimens from 21 oral red and/or white lesions (6 hyperkeratosis, 3 mild dysplasia, 4 severe dysplasia and 8 squamous cell carcinoma), using monoclonal antibodies and avidin-biotin-peroxidase complex staining. Peripheral blood samples (PB) from 4 normal subjects and 5 reactive lymph nodes (LN) were used as controls for the technique. T11-positive cells were the predominant phenotype (74-78%) in all cases examined. The T4/T8 ratio in severe dysplasia was significantly lower than that in mild dysplasia (p less than or equal to 0.05). These observations support the hypothesis of a role for cellular immune responses in oral premalignant lesions and oral cancer. The predominance of T cells may represent the local expression of immunity against antigens (viral or other). The decreased T4/T8 ratio observed in severe dysplasia may represent a transitory stage of local immunosuppression, which may be of critical importance for the progression into carcinoma. Phenotypic variations in mononuclear cell infiltrates in these conditions could be diagnostic value.

Adult↗
PubMed1986

Infectious and sexually transmitted diseases: implications for dental public health.

The Acquired Immunodeficiency Syndrome (AIDS) has stimulated renewed attention toward infectious diseases and dental public health. Currently, AIDS is defined as individuals with Kaposi's sarcoma and/or pneumocystis carinii pneumonia and/or other life-threatening opportunistic infections (e.g., specific forms of tuberculosis or meningitis, candidal esophagitis), and associated immunosuppression that cannot be accounted for by another disease process and/or medications. As of January 1986, the AIDS epidemic has afflicted over 16,000 persons in the United States and has taken over 8,000 lives. All present indications point toward a much more extensive epidemic based on the lack of a vaccine and effective forms of treatment, the large number of carriers in the population, and variable modes of transmission.

Acquired Immunodeficiency Syndrome↗
PubMed1986

Oral findings in people with or at high risk for AIDS: a study of 375 homosexual males.

A total of 375 homosexual males were studied to assess the dental findings, life-style, and risk factors during a 4-year period. At baseline, 136 of the patients were diagnosed as having AIDS, 116 were considered at risk for AIDS, and 123 were considered healthy. In a mean follow-up time of 23 months, nine of the patients at risk for AIDS and five of the patients considered healthy were diagnosed as having AIDS. Kaposi's sarcoma was the most common oral neoplasm, and candidiasis was the most frequent oral infection. Hairy leukoplakia was found in 28% of the patients, and periodontal disease was found in 17% of the patients. Carriers of the AIDS virus may not be identified easily and control measures in the dental office must be followed.

Acquired Immunodeficiency Syndrome↗
PubMed1986

Examination of oral cancer tissue for the presence of the proteins ICP 4, ICP 5, ICP 6, ICP 8, and gB of herpes simplex virus type 1.

Although patients with oral cancer have increased levels of antibody to herpes simplex virus type 1, the origin of the antigenic stimulation remains unknown. We have therefore looked for proteins of herpes simplex in oral squamous cell carcinomas by staining frozen sections with monoclonal antibodies to the proteins ICP 4, ICP 5, ICP 6, ICP 8, and gB. No staining was seen of the tumor cells of any of 11 oral cancer cases or of the epithelium of 29 other oral lesions, which included cases of leukoplakia, lichen planus, and aphthous ulcers. Frequent staining of mast cells was seen in the connective tissue associated with oral cancer when ascitic fluid was used as the source of monoclonal antibody, but such staining was not seen when the precipitated IgG fraction was used.

Antibodies, Monoclonal↗
PubMed1985

Clinical efficacy of prednisone in the treatment of patients with oral inflammatory ulcerative diseases: a study of fifty-five patients.

An objective evaluation of 55 patients with chronic vesiculoerosive oral diseases was made to compare the beneficial and adverse effects of prednisone treatment. Patients were divided into groups of high, intermediate, and low drug dosages, as well as short and long periods of administration. Prednisone benefited 49 patients in spite of the fact that 55% had some adverse side effects. The duration of prednisone administration appeared to have a larger impact on side effects than the daily amount given. The most common side effects were gastrointestinal disturbances, mood alterations, polyuria and insomnia. There were no significant changes in blood pressure, blood sugar level, weight, or white blood cell counts. This study confirmed that systemic prednisone is a useful and beneficial treatment modality for patients with chronic oral inflammatory diseases.

Adolescent↗
PubMed1985

A prospective follow-up study of 570 patients with oral lichen planus: persistence, remission, and malignant association.

Five hundred seventy patients with oral lichen planus were followed for periods ranging from 6 months to more than 10 years (mean, 5.6 years). The mean age was 52 years, and 67% of the patients were women. Erosive lichen planus was the most frequent clinical form, and the buccal mucosa was the most common site. Of the 75% patients treated with corticosteroids, 29% experienced complete remission and 63% had partial remission while maintained on medication. Fewer than 3% experienced spontaneous remission. Malignant transformation occurred in 7 patients (1.2%) in a mean time of 3.4 years after the onset of lichen planus. The onset of lichen planus could not be associated with any evident factors, such as family history, Candida albicans, glucose intolerance, and smoking.

Adolescent↗
PubMed1985

Proliferative verrucous leukoplakia. A long-term study of thirty patients.

Up to 6% of oral leukoplakia, a relatively common mucosal disease, can be expected to become malignant. This report describes a long-term study of 30 patients in whom a particular form of leukoplakia was identified and labeled proliferative verrucous leukoplakia (PVL), a disease of unknown origin, which exhibits a strong tendency to develop areas of carcinoma. PVL begins as a simple hyperkeratosis but tends to spread and become multifocal. PVL is slow-growing, persistent, and irreversible, and in time areas become exophytic, wartlike, and apparently resistant to all forms of therapy as recurrence is the rule. The disease was most commonly seen in elderly women and had been present for many years. Patients were followed for 1 to 20 years. Thirteen died of or with their disease, 14 were alive with PVL, and 3 were alive without PVL at last contact. PVL rarely regressed despite therapy. All patients who died had persistent or recurrent disease. PVL appears to constitute a continuum of hyperkeratotic disease, ranging from a simple hyperkeratosis at one end to invasive squamous cell carcinoma at the other. Microscopic findings are dependent upon the stage of the disease's development and the location and adequacy of the biopsy.

Adult↗