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Biomedical subjects

S Shibata

Publications and source records attributed to S Shibata.

At least 1,009 records · Page 56Linked to original sources
PubMed1975

Nephritogenic glycoprotein. VI. Induction of proliferative glomerulonephritis with the immunofluorescent 'mesangial pattern' in rats by a single injection of soluble glycoprotein isolated from human urine.

Proliferative glomerulonephritis was induced in rats by a single injection in the hind footpads of the urinary glycoprotein or more purified urinary glycopeptide with Freund's incomplete adjuvant. Although the urinary (water-soluble and non-dialyzable) glycoprotein and glycopeptide were prepared from heterologous urines (human urines), the immunofluorescent pattern of the kidneys was not 'granular' but 'mesangial', as in rats given a single injection of homologous or heterologous renal glycoprotein with nephritogenicity. The pathogenesis of proliferative glomerulonephritis in the present experiments may be explained by the same mechanism as that of rats given a single injection of renal glycoprotein, i.e. by any mechanism other than antigen (heterologous protein)-antibody immune complex and anti-GBM antibody mechanisms.

Animals↗
PubMed1974

Effect of 6-hydroxydopamine on [3H]-adenine nucleotide and [3H]-noradrenaline release from the guinea-pig taenia caecum evoked by electrical field stimulation, nicotine and perivascular nerve stimulation.

1 The effects of pretreatment of guinea-pigs with 6-hydroxydopamine (6-OHDA) on the release of [(3)H]-noradrenaline and [(3)H]-adenine nucleotide following electrical field, nicotine and perivascular nerve stimulation of guinea-pig taenia caecum were studied.2 High frequency electrical field stimulation (15,30 Hz) released both [(3)H]-noradrenaline and [(3)H]-adenine nucleotide but low frequency (0.5, 5 Hz) stimulation, producing comparable muscle relaxation led only to [(3)H]-noradrenaline release.3 6-OHDA (50-200 mg/kg) pretreatment inhibited the muscle relaxation and [(3)H]-noradrenaline release with electrical stimulation or with nicotine in isolated taenia but did not affect the release of [(3)H]-nucleotide.4 A low dose of 6-OHDA (50 mg/kg), completely inhibited the muscle relaxation and [(3)H]-noradrenaline release elicited by perivascular nerve stimulation.5 Both tissue noradrenaline content and [(3)H]-noradrenaline uptake were decreased to the same extent by low as well as high doses of 6-OHDA: noradrenaline content was reduced to 20% and uptake to 30% of the control value.6 Catecholamine fluorescence disappeared from tissue layers of the taenia after treatment with a high dose of 6-OHDA.7 In these experiments the inhibitory action of electrical stimulation and nicotine on the taenia can be correlated better with noradrenaline than with nucleotide release.

Adenine Nucleotides↗