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Biomedical subjects

S Shibata

Publications and source records attributed to S Shibata.

At least 955 records · Page 53Linked to original sources
PubMed1977

Nephritogenic glycoprotein. V. Immunochemical studies on nephritogenic activity of collagenase and pronase-digests prepared from various rat organs.

A new immunochemical procedure was introduced to estimate the nephritogenic activity of collagenase and pronase digests of various rat organs. A glycoprotein isolated from collagenase digests of various rat organs showed the nephritogenic activity as well as the antigenic activity that induces nephrotoxic antibody, which were nearly identical to those of a glycoprotein isolated from trypsin digests of the rat organ concerned. A glycoprotein isolated from pronase digests of various rat organs was proved to have no antigenic activity that induces nephrotoxic antibody, but the existence of nephritogenic activity was proved in this glycoprotein. These results were supported firmly by the assay experiments for nephritogenicity. Ouchterlony gel diffusion test and the assay experiments demonstrated the existence of a common nephritogenic substance among the glycoproteins isolated from trypsin, collagenase and pronase digests of rat organs.

Animals↗
PubMed1977

Lactose synthase activity of galactosyltransferase from human parotid saliva.

Galactosyltransferase is human parotid saliva catalyzed the transfer of galactose from uridine 5'-diphospho (UDP)-galactose to D-glucose in the presence of exogenous alpha-lactalbumin. Some albumins other than alpha-lactalbumin did not have an effect on the appearance of lactosesynthesizing activity.

Galactosyltransferases↗
PubMed1977

Glycoproteinuria as an indicator of glomerular basement membrane damage.

Urinary glycoprotein was isolated from human urine by the use of the same procedures as those used for isolation of human renal glycoprotein from the glomerular basement membrane (GBM). In Ouchterlony plates and immunoelectrophoretically, human urinary glycoprotein has a common structural component with human renal glycoprotein isolated from GBM. Massive excretion of the glycoprotein in the urine was associated with only two diseases, systemic lupus erythematosus (membranous type) and membranous glomerulonephritis, although it was isolated from the urine of patients with renal as well as nonrenal diseases and normal subjects. In these two membranous diseases, the glycoprotein of GBM could be intensively liberated into the urinary space, as the result of extensive damage of GBM that is initiated by immune-complex deposit. It is discussed that urinary content of this glycoprotein may indicate the degree of the GBM damage in these diseases.

Basement Membrane↗
PubMed1977

Effect of phthalazinol (EG-626) on the mechanical activity and calcium influx of rabbit portal vein (1).

Phthalazinol (EG-626), a newly synthesized cyclic AMP phosphodiesterase inhibitor, inhibited spontaneous phasic contractions (SPC) of isolated rabbit portal veins in a dose-dependent manner. Phthalazinol at a minimal concentration necessary to completely abolish the SPC did not alter 45Ca-uptake, 45Ca-influx, or the ED50S for methoxamine (me) and K+, but it did reduce the amplitude of the tonic contractions induced by ME and K+ by approximately 25%. On the other hand, verapamil, and La+3 abolished SPC, ME, and K+-induced contractions. Tetrodotoxin had no effect on SPC or on tonic contractions induced by ME and K+. Since extracellular Ca++ is known to be essential for SPC, ME, and K+-induced contractions in rabbit portal vein, the present results suggest that the abolishment of SPC and the partial inhibition of the ME and K+ responses by phthalazinol may be related to an action of this agent on intracellular Ca++ or Ca++-efflux.

Animals↗