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Biomedical subjects

S Schubert

Publications and source records attributed to S Schubert.

At least 109 records · Page 6Linked to original sources
PubMed1990

[Immunoenzyme assay of parasite-specific IgG4 antibodies in schistosomiasis using the monoclonal antibody BL-IgG4/1].

Sera from 251 children living in endemic areas (Schistosoma mansoni or Schistosoma haematobium) and from 188 hospital outpatients in Ethiopia were evaluated for specific IgG4 antibodies reacting with Schistosoma mansoni adult worm antigen employing an enzyme-immunoassay. Patients with schistosomiasis (n = 140) possessed a significantly higher mean value of specific IgG4 antibodies than normal controls (n = 30) and individuals from different countries who had no schistosomiasis but are infected with other parasites (n = 114). Blood samples dried on filter paper were also acceptable in these test. The use of the test in diagnosis is compared and assessed with parasitological methods.

Animals↗
PubMed1990

[Enzyme immunoassay of the level of parasite-specific IgE antibodies in schistosomiasis using the monoclonal antibody BL-IgE 9].

Sera from patients with chronic schistosomiasis (Schistosoma mansoni or Schistosoma haematobium) were examined for the presence of parasite specific IgE antibodies by means of ELISA technique using tegument antigen prepared from adult worms of Schistosoma mansoni and using the monoclonal antibody BL-IgE 9. Individuals from tropical countries who had no schistosomiasis and blood donors from GDR were studied for comparison. Significantly higher levels of specific IgE antibody were given by sera from patients with schistosomiasis than by the controls. These differential responses serologically differentiated between patients with chronic schistosome infections and noninfected individuals.

Antibodies, Helminth↗
PubMed1989

Field studies towards intestinal helminthoses from north Ethiopia (1982) by means of microscopic and seroimmunologic methods.

In two villages of north Ethiopia (Gorgora and Zerema) helminthologic stool analyses were performed and compared with seroimmunologic examinations. Regional difference could be obtained. The infection rate of males was slightly higher than that of females (except Schistosomiasis in Gorgora). Ascaridiasis, Ancylostomiasis and Trichuriasis are probably life-long accompanying parasitoses--they were slightly more frequent in higher than in lower age in Zerema--in contrast to Schistosomiasis which was slightly more frequent in younger people (differences not significant, likely due to the small number of examined persons). Toxocariasis in man is probably not very common in this region. The values of such pilot studies and of seroimmunologic findings for epidemiologic field investigations are discussed.

Adolescent↗
PubMed1989

[Results and experiences with several methods for the immunodiagnosis of amebiasis].

Results and experiences of some immunodiagnostic methods for amebiasis. Experiences concerning the laboratory diagnosis of invasive amebiasis are described and discussed. Technics used include the Passive Hemagglutination Test with E. histolytica antigen (Behring FRG) and sheep erythrocytes as carrier and the Indirect Fluorescence Antibody Reaction (IFAR) with corpuscular antigen prepared from cultures of E. histolytica strain HK 9. Comparatively for a part infected hamster liver (Dr. Richle strain) has been used as antigen. We conclude that IFAR with HK 9 antigen is more sensitive than the other tests. It has been proved useful in the diagnosis of extraintestinal amebiasis (antibody titer greater than or equal to 1:160).

Amebiasis↗
PubMed1986

Complex control of schistosomiasis.

The main problems of complete Schistosomiasis control in endemic areas are eradication of intermediate host snails, large-scale treatment and health education of the population. Health education seems to be the most important factor in maintaining the standard of control. The introduction of very cheap, effective and fast screening methods and the development of very efficacious and easily applicable drugs have made large-scale treatment one of the most important methods of epidemiological combat. The eradication of snails has lost importance, with chemical and, to a growing extent, biological means of snail control becoming the focus of interest. In view of recent success in combating Schistosomiasis, it can be assumed that this disease will cease to be a major threat to public health in endemic areas.

Animals↗
PubMed1985

Active extrusion of protons into deionized water by roots of intact maize plants.

The investigations were focussed on the question as to whether roots of intact maize plants (Zea mays L. cv Blizzard) release protons into deionized H(2)O. Plants in the six to seven leaf stage depressed the pH of deionized H(2)O from 6 to about 4.8 during an experimental period of 4 hours. Only one-third of the protons released could be ascribed to the solvation of CO(2) in H(2)O. The main counter anions released were Cl(-), NO(3) (-), and SO(4) (2-). At low temperature (2 degrees C), the H(+) release was virtually blocked while a relatively high amount of K(+) was released. The presence of K(+), Na(+), Ca(2+), and Mg(2+) in the external solution increased the H(+) secretion significantly. Addition of vanadate to the outer medium inhibited the H(+) release while fusicoccin had a stimulating effect. Substituting the nutrient solution of deionized H(2)O resulted in a substantial increase of the membrane potential difference from -120 to -190 millivolts. The experimental results support the conclusion that the H(+) release by roots of intact maize plants is an active process driven by a plasmalemmalocated ATPase. Since the net H(+) release was not associated with a net uptake of K(+), it is unlikely to originate from a K(+)/H(+) antiport.

Journal Article↗
PubMed1984

Remarks on gastroenterologic diagnostics of amoebic liver abscess, peritoneal tuberculosis and tropical splenomegaly.

The amoebic liver abscess, the peritoneal tuberculosis and the "tropical splenomegaly" belong to diseases which are frequent in warm countries but single cases appear also in moderate climate by intercontinental communications. False diagnoses especially in the first both can lead to severe rsp. fatal consequenses. Therefore experiences are dealed with, which have been gained within nearly one year in Ethiopia and which shall illustrate the diagnostic procedures under field conditions in warm countries as well as under conditions of modern hospitals in moderate climates. Amoebic liver abscess is quickly detected and confirmed by sonography and immunodiagnostic. Contrary to it in the fields there is the puncture by means of a i. v.-needle often not only the sole diagnostic but also the first and sometimes life-saving therapeutic procedure in advanced cases. It allows X-ray in double contrast technique if equipment is available. In suitable cases such approved technique shall be used also in modern clinics disposing of methods like sonography and computerized tomography. Concerning peritoneal tuberculosis our examinations agreed with the opinion of literature that the laparoscopy seems to be the most suitable method for quickly detection and safe discrimination from decompensated portal hypertension in liver cirrhosis. By the way the "cope needle" are mentioned which is distributed in warm countries for searching for peritoneal tuberculosis by performance of a blind peritoneal biopsy. It is easier than laparoscopy but not so efficient. - In this connection 31 cases examined by laparoscopy are presented in a table in order to give an impression on further indications to laparoscopy existing in such areas of warm countries.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1983

[Current status of malaria in Ethiopia].

During the least years also in Ethiopia the number of cases of malaria increased despite intensive control measures. There does not yet exist a chloroquine resistance, but, however, special attention must be paid, since it became known from other East-African countries that there were chloroine resistances. DDT has a still sufficient effect. The most important carrier anophelines are A. gambiae S. 1, A. pharoensis, A. funestus and A. nili. M. tropica and M. tertiana nearly exclusively occur. The severest lesions are caused by M. tropica, when it affects malnourished population groups. In contrast to other descriptions a risk of malaria is temporarily existing also at an attitude of more than 2,000 m, though it is here clearly more insignificant than in lower regions. a seasonal accumulation is found from September to November. In contrast to the high rate of incidence of the native population in endemic districts the number of diseases GDR citizens in Ethiopia was extremely low in recent years. Chloroine is further on recommended for medicamentous prophylaxis.

Ethiopia↗
PubMed1983

[Assessment of late complications of malaria in travelers to the tropics].

Irreparable late lesions after a malaria infection are rare. They occur mainly after infection of tropical malaria. As ascertained late lesions are regarded cerebral disturbances, e.g. epilepsy after cerebral tropical malaria, cardiac disturbances, e.g. arrhythmia after cardiac tropical malaria and chronic renal insufficiency after quartan malaria. Chronic liver diseases, e.g. cirrhosis, are not known as ascertained late lesions of malaria. Bodily lesions may be recognized by experts as malaria-depending, when kind and form of the course of malaria may be considered as cause meanwhile appearing symptoms anticipate a connection and other causes were excluded with regard to these lesions.

Epilepsy↗