[The diagnostic localization of acute gastrointestinal bleeding in a primarily negative angiographic finding].
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Acute Disease↗
Biomedical subjects
S Schmitt
Publications and source records attributed to S Schmitt.
Tsukamurella tyrosinosolvens sp. nov.
Chemotaxonomic and 16S ribosomal DNA sequence analyses of four bacterial isolates from blood cultures from patients with cardiac pacemaker implants and sputa of patients with chronic lung infections clearly demonstrated that these bacteria belong to the genus Tsukamurella. DNA-DNA hybridization data, as well as the physiological characteristics of the isolates, indicate that they are closely related and belong to a single species that differs from previously described members of the genus Tsukamurella. The name Tsukamurella tyrosinosolvens sp. nov. is proposed for these isolates, and the new species is represented by strain IMMIB D-1397T (= DSM 44234T). Strain IMMIB D-1397T exhibits 53.4, 53.5, and 54.7% DNA-DNA relatedness to Tsukamurella paurometabola DSM 20162T, Tsukamurella inchonensis DSM 44067T, and Tsukamurella pulmonis DSM 44142T, respectively.
Actinomycetales↗
Recombinant plasmid mobilization between E. coli strains in seven sterile microcosms.
Transfer by mobilization of a pBR derivative recombinant plasmid lacking transfer functions (oriT+, tra-, mob-) from one E. coli K12 strain to another was investigated in seven sterile microcosms corresponding to different environments. These microcosms were chosen as representative of environments that genetically engineered microorganisms (GEMOs) encounter after accidental release, namely attached biomass in aquatic environments (biofilm), soil, seawater, freshwater, wastewater, mouse gut, and mussel gut, GEMOs survived in the same way as the host strains in all microcosms. Recombinant DNA mobilization occurred in the mouse gut, in sterile soil, and in biofilm. The plasmid transfer rates principally reflected the environmental conditions encountered in each microcosm.
Animals↗
High molecular weight forms of IGF-II ('big-IGF-II') released by Wilms' tumor cells.
Insulin-like growth factor-II (IGF-II) is thought to play a critical role in the development of embryonic tumors such as Wilms' tumor. However, despite highly elevated IGF-II mRNA levels in tumors, IGF-II is not elevated in the serum of patients with Wilms' tumors. Recently high molecular weight forms of IGF-II ('big'- or pro-IGF-II) have been found to be produced by some tumors. In order to prove whether or not high molecular weight forms of IGF-II are produced by Wilms' tumor cells and secreted into the culture medium, we established Wilms' tumor cell lines. After column chromatography of the culture medium, IGF-II and pro-IGF-II concentrations were measured. For pro-IGF-II measurement we established a pro-IGF-II RIA using a rabbit polyclonal antiserum directed against amino acids 7-21 (E7-21) of the E-domain of pro-IGF-II. Gel electrophoresis and Western blotting with anti-IGF-II antibodies revealed a band at 7.5 kDa corresponding to fully processed IGF-II and bands between 10 and 20 kDa. Using pro-IGF-II antiserum, bands between 10 and 25 kDa were detected. We conclude that in vitro cultured Wilms' tumor cells produce and release various forms of 'big IGF-II' with molecular masses between 10 and 25 kDa. It remains uncertain whether these high molecular weight forms of IGF-II represent normal precursors of IGF-II or incorrectly processed IGF-II.
Animals↗
[2-Aryl-pyrrolo[2,1-b]benzothiazoles as a selective or dual inhibitors of cyclo-oxygenases and 5-lipoxygenases. 21. Non-steroidal anti-inflammatory agents].
A series of 2-aryl-pyrrolobenzothiazoles with additional functional groups was synthesized and characterized. Mainly ring opening and less substitution of the pyrrole moiety was observed reacting the heterocyclic system with diazoethyl acetate. In general all compounds inhibit 5-lipoxygenase more effectively than cyclooxygenase but one of them is a well balanced dual inhibitor. A structure-activity relationship and the enzyme selectivity are discussed.
Anti-Inflammatory Agents, Non-Steroidal↗
[Correlation between postoperative duration of Redon drainage and wound healing. A study of 150 patients with total endoprosthetic hip replacement].
The influence of the residual application of postoperative wound suction drainage devices on wound healing was examined in 150 patients with total hip replacement. The duration of continuous wound-draining as well as their bacterial contamination were analysed and the possible correlation was evaluated. Another aim of the study was to observe if the bacteriologic result is a proper method to obtain evidence about the wound situation. The 150 patients were divided in 3 groups. Two groups (of 50 patients each) had total hip replacement at the same time period. One group had postoperative wound complications or infection that had to be reoperated. The other 50 patients had normal wound healing without any complication. The third group of 50 patients with a shorter draining period was analysed and compared with the others. As a control factor the indication for operation, the sex and the age of the patients were similar in each group. We could show an increasing number of bacteriological contamination in drainage tubes which had a longer suction period than 3 days. In the reoperated group we found a significantly higher number of contaminated tips (37.4%) than in the groups with uncomplicated wound healing (8% and 2.8%). The comparison of the bacteriological examination of the drainages with the smear of reoperated wounds showed the same constellation of germs in 64.3% of the cases. It seems to be a good method to get information about the bacterial wound conditions.
Aged↗
Identification of a contact region for peptide on the TAP1 chain of the transporter associated with antigen processing.
The transporter associated with Ag processing (TAP) translocates cytosolic peptides into the endoplasmic reticulum for presentation by MHC class 1 molecules. Recently, the actual peptide translocation step has been suggested to be preceded by binding of the peptide to TAP. In this study, we investigated the peptide binding site of TAP and its relevance for peptide selection by cross-linking of translocatable peptides. Our data demonstrate, first, that for a TAP heterodimer containing the rat TAPu allelic product, which selects peptides on basis of their C terminus, the translocation efficiency correlates with the peptide binding efficiency. Second, peptides having the cross-linker at different positions all label both the TAP1 and the TAP2 subunit after binding to the heterodimer, indicating that both TAP subunits contribute directly to the peptide binding site and contact most or all amino acids of a bound peptide. Third, by enzymatic digestion and the use specific antisera, we identified a domain of human TAP1 that contributes to the peptide binding site. This domain contains the two hydrophobic and thus putative transmembrane regions closest to the ATP binding sites. We conclude that the peptide binding site controls the selectivity of TAP and is composed of domains of both TAP1 and TAP2, which each contact the bound peptide over all or most of its length. Moreover, the major contact site(s) for peptide on TAP1 are located within or close to the two putative transmembrane regions adjacent to the ATP binding site.
ATP Binding Cassette Transporter, Subfamily B, Mem↗
The effect of simulated function on the retention of bar-clip retained removable prostheses.
Patients wearing bar-clip retained removable prostheses may have loss of retention because of changes within the bar-clip assembly. This in vitro study recorded and compared the retention of one- and two-clip retained simulated mandibular complete denture prostheses before and after simulated function. Cast metal Hader bars and clip holders were used to make 10 one-clip and 10 two-clip specimen pairs. Tensile removal values before and after simulated function were recorded and compared by repeated-measures analysis of variance and Student tau tests (significance level 0.05). The results revealed that the use of two clips instead of one significantly increased retention of the simulated prosthesis. It was also found that there was a significant loss of retention after the specimens were placed on the bars and then removed once for both the one- and two-clip groups. Simulated function did not cause a significant change in retention for either group.
Analysis of Variance↗
Inducible NO synthase II and neuronal NO synthase I are constitutively expressed in different structures of guinea pig skeletal muscle: implications for contractile function.
The expression of NOS isoforms was studied in guinea pig skeletal muscle at the mRNA and protein level, and the effect of NO on contractile response was examined. Ribonuclease protection analyses demonstrated NOS I and NOS II mRNAs in diaphragm and gastrocnemius muscle. In Western blots, NOS I and NOS II immunoreactivities were found in the particulate but not the soluble fraction of skeletal muscle. NOS activity was found almost exclusively in the particulate fraction. About 50% of this activity was Ca2+ independent. In immunohistochemistry, the anti-NOS I antibody stained distinct membrane regions of muscle fibers. The most intense staining was seen in neuromuscular endplates identified by labeling with alpha-bungarotoxin. The anti-NOS II antibody labeled muscle fibers that contained alkali-labile myosin ATPase (type I fibers). NOS II was located to intracellular structures and was also seen in "specific pathogen-free" animals. Pretreatment of guinea pigs with bacterial lipopolysaccharide (LPS) markedly intensified NOS II staining. Significant NOS III immunoreactivity was detected only in vascular endothelium. In functional experiments, tetanic muscle contractions were induced in diaphragm and gastrocnemius muscle by electrical stimulation of the innervating nerves. Pretreatment of guinea pigs with LPS or addition of S-nitroso-N-acetyl-D,L-penicillamine to the organ bath markedly decreased tetanic contractions. N(G)-nitro-L-arginine, on the other hand, increased contractile force and reversed the effect of LPS. Our data indicate that NOS II and NOS I are expressed in different structures of skeletal muscle and are involved in the regulation of contractile response.
Animals↗
Autocrine regulation of growth by insulin-like growth factor (IGF)-II mediated by type I IGF-receptor in Wilms tumor cells.
Wilms tumor is a common embryonic tumor in childhood. Two Wilms tumor-suppressor genes, WT1 and WT2, are located on chromosome 11p, WT2 at 11p15.5 close to the IGF-II gene, which is highly expressed in some Wilms tumors. We established Wilms tumor cell lines to investigate the regulation of tumor cell growth by IGF-II. We demonstrated that Wilms tumor cells produce more IGF-II than normal kidney cells. Both types I and II IGF receptors reside on these cells. In serum-free culture medium, tumor cell growth is reversibly inhibited by suramin via interfering with IGF-II binding. Wilms tumor cell growth is also arrested by IGF binding protein-3, capturing the continuously produced IGF-II, and by alpha IR-3, a type I IGF receptor-blocking antibody. Thus, we demonstrated the whole loop of elevated synthesis, secretion, receptor binding, and autocrine growth stimulation of IGF-II through type I IGF receptor in Wilms tumor cell cultures. We concluded that IGF-II plays a crucial role in the regulation of growth of this embryonic tumor. Overproduction of IGF-II by the tumor cell is the limiting step for Wilms tumor growth, supporting its important role as an embryonic growth factor.
Animals↗
[Nursing aspects of flexible bronchoscopy in premature and newborn infants].
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Bronchoscopy↗
Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site.
In the framework of the European BIOTECH project for sequencing the Saccharomyces cerevisiae genome, we have determined the nucleotide sequence of the left part of the cosmid clone 232 and the cosmid clone 233 provided by F. Galibert (Rennes Cedex, France). We present here 33,099 base pairs of sequence derived from the left arm of chromosome X of strain S288C. This sequence reveals 17 open reading frames (ORFs) with more than 299 base pairs, including the published sequences for ARG3, LIGTR/LIG1, ORF2, ACT3 and SCP160. Two other ORFs showed similarity with S. cerevisiae genes: one with the CAN1 gene coding for an arginine permease, and one with genes encoding the family of transcriptional activators containing a fungal Zn(II)2-Cys6 binuclear cluster domain like that found in Ppr1p or Ga14p. Both putative proteins contain a leucine zipper motif, the Can1p homologue has 12 putative membrane-spanning domains and a putative alpha 2-SCB-alpha 2 binding site. In a diploid disruption mutant of ORF J0922 coding for the transcriptional activator homologue, no colonies appeared before 10 days after transformation and then grew slowly. In contrast, haploid disruption mutants showed a growth phenotype like wild-type cells. One ORF showed weak similarity to the rad4 gene product of Schizosaccharomyces pombe and is essential for yeast growth. Five ORFs showed similarity to putative genes on the right arm of chromosome XI of S. cerevisiae. Two of them have similarity to each other and belong to a family of extracellular proteins that groups mammalian SCP/Tpx-1, insects Ag3/Ag5, plants PR-1 and fungi Sc7/Sc14.(ABSTRACT TRUNCATED AT 250 WORDS)
Chromosomes, Fungal↗
Oral versus repository corticosteroid therapy after hospitalization for treatment of asthma.
Tapering regimens of oral steroids may be difficult or confusing for some patients. Repository steroids have been shown to be as effective as tapering oral doses in preventing relapse after emergency treatment. This study was undertaken to determine whether repository steroids are as effective as tapering oral steroids in preventing relapse after hospitalization for treatment of asthma. Twenty-six patients with acute exacerbations of asthma requiring treatment for 24 to 72 hours with parenteral steroids were randomized into two groups. Both groups received oral prednisone, 60 mg daily, when parenteral steroids were discontinued. At discharge, one group received intramuscular placebo and oral prednisone tapered over 8 days, and the other received 80 mg intramuscular methylprednisolone sodium acetate and oral placebo. At discharge and 2-week follow-up, patients were interviewed and examined, and spirometry results were obtained. There was little difference between groups in ratings of symptoms at discharge or follow-up. Both groups had less wheezing at follow-up than at discharge, though the improvement was significant only in the repository steroid group (p < 0.05). Mean forced expiratory volume in 1 second, forced vital capacity, peak expiratory flow rate improved at follow-up in both groups. No significant differences in outcome were found between the oral and repository steroid groups.
Acute Disease↗
Uniparental disomy 7 in Silver-Russell syndrome and primordial growth retardation.
Maternal uniparental disomy for the entire chromosome 7 has so far been reported in three patients with intrauterine and postnatal growth retardation. Two were detected because they were homozygous for a cystic fibrosis mutation for which only the mother was heterozygous, and one because he was homozygous for a rare COL1A2 mutation. We investigated 35 patients with either the Silver-Russell syndrome or primordial growth retardation and their parents with PCR markers to search for uniparental disomy 7. Four of 35 patients were found to have maternal disomy, including three with isodisomy and one with heterodisomy. The data confirm the hypothetical localization of a maternally imprinted gene (or more than one such gene) on chromosome 7. It is suggested to search for UPD 7 in families with an offspring with sporadic Silver-Russell syndrome or primordial growth retardation.
Chromosome Aberrations↗
Sequence and function analysis of a 9.74 kb fragment of Saccharomyces cerevisiae chromosome X including the BCK1 gene.
In the framework of the European BIOTECH project for sequencing the Saccharomyces cerevisiae genome, we have determined the nucleotide sequence of the cosmid clone 233 provided by F. Galibert (Rennes Cedex, France). We present here 9743 base pairs of sequence derived from the left arm of chromosome X. This sequence reveals three new open reading frames and includes the published sequence (5' end and open reading frame) of the gene BCK1/SLK1/SSP31 also identified as ORFAA. Deletion mutants of two earlier unknown open reading frames J0840 and J0904 are viable and the open reading frame J0902 is essential for yeast growth.
Amino Acid Sequence↗
Gonadotropin regulation during puberty in complete androgen insensitivity syndrome with testicles in situ.
This paper describes the time course of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels in two patients with the complete androgen insensitivity syndrome (AIS) during puberty and adolescence prior to gonadectomy. In early puberty, LH values were comparable to normal male as well as female standards. At the age of 17 and 18.7 years, respectively, a sudden rise in LH occurred just after the start of estradiol supplementation. These high LH concentrations in the late puberty of our patients are comparable to the values found during early puberty in castrated individuals and children with gonadal dysgenesis. In contrast, FSH concentrations continuously showed levels in the normal male or female range with no rise during adolescence. The sudden increase of LH in late puberty is most probably due to defective testosterone receptors in the pituitary/hypothalamus which apparently can no longer be suppressed by rising testosterone levels and an escape from the negative estradiol feedback. The role of estradiol supplementation might be incidental and further investigation into its role is needed. In contrast to the elevated levels of FSH in patients with defective spermatogenesis, FSH is normal in our patients with AIS and testes in situ. This indicates that FSH secretion is regulated by a combined action of estradiol and gonadal hormones like inhibin.
Adolescent↗
[Preoperative and postoperative care of children with esophageal atresia].
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Esophageal Atresia↗
Eupergit C-coated membranes as solid support for a sensitive immunoassay of human albumin.
A number of commercially available membranes preactivated by coating with oxirane-containing Eupergit C beads were used for diagnostic immunoassay applications. These membranes possess a higher capacity for protein binding than the respective unmodified membranes as measured with fluorescently labelled antibodies. Immobilization of antigens or antibodies as the first step of the assay was achieved by covalent binding of amino groups of proteins to the oxirane moieties introduced onto the membranes. The high performance of the newly developed membranes bearing covalently bound antibodies, was demonstrated by a dot enzyme-linked microalbuminuria immunoassay as compared to unmodified membranes.
Albumins↗