Biomedical subjects

S Sano

Publications and source records attributed to S Sano.

At least 127 records · Page 7Linked to original sources

PubMed1995

[Comparison between the arthroscopic and open synovectomies for rheumatoid knee--a retrospective and random study on the results of the two methods].

Knee joints affected by rheumatoid arthritis (RA) were randomized to either arthroscopic or open synovectomy, and the postoperative results were compared retrospectively. The materials consisted of 58 patients with 75 surgically treated knees. Arthroscopic synovectomy was performed on 37 knees of 30 patients, who were followed for five years and four months on average. Open synovectomy was performed on 38 knees of 28 patients, who were followed for five years and five months on average. The results were evaluated for ; recurrence of synovitis ; duration of the symptom-free period ; progressive bone changes on radiography ; range of motion (ROM) ; duration of hospital stay ; duration of surgery. Due to the less invasive surgical procedure, arthroscopic synovectomy resulted in less post operative pain, and there was no restriction of ROM after surgery, no need for postoperative rehabilitation, shorter hospital stay, lower incidence of osteoarthritic changes, and earlier return to the daily life. However, as synovial membrane removal was unavoidably insufficient, the effect of surgery lasted for only 18 months on average and recurrence of synovitis was observed in 73 % of patients. Open synovectomy meant relatively greater invasion and postoperative pain. Due to the restricted ROM in some cases, postoperative treatment was required and the hospital stay tended to be prolonged. Nevertheless, the recurrence rate was only 37 % during follow-up and the effect of surgery persisted for an average of 75 months, much better than that of arthroscopic synovectomy. In the treatment of RA knees, the indications for arthroscopic and open synovectomies should be carefully studied in the light of the merits and for demerits of each procedure.

Adult↗

PubMed1994

Nonpeptide ligands for human gamma delta T cells.

gamma delta T cells respond to a variety of microbial pathogens and transformed cells. Their limited receptor repertoire and activation by mycobacterial antigens resistant to proteases suggest that they may recognize nonpeptide antigens. We have tested a variety of nonpeptide molecules for stimulation of human gamma delta T cells. Synthetic alkyl phosphates, particularly monoethyl phosphate (MEP), selectively activated gamma delta T cells and stimulated their proliferation in vitro. All gamma delta T cells stimulated by MEP expressed V gamma 2/V delta 2 receptors. The purified natural ligand of mycobacteria is chemically similar to, though distinct from, MEP and contains a phosphate residue that is critical for biological activity. Recognition and expansion of a specific T-cell receptor-bearing population to non-peptide ligands is unprecedented among T cells. We suggest that MEP mimics small natural ligands capable of expanding one subset of gamma delta T cells and that this recognition of nonpeptide antigens may play an important role in human immunity to pathogens.

Antibodies↗

PubMed1994

Micropathologic changes of pars tensa in children with otitis media with effusion.

Clinical studies have supported a relationship between otitis media with effusion in children and chronic otitis media in adults. Although clinical studies are numerous, relatively little is known about the histopathologic changes of the tympanic membrane in otitis media with effusion. Tympanic membranes were taken from the intermediate zone of the anteroinferior quadrant of the tympanic membrane during surgery for placement of tympanostomy tubes in 30 children (age range, 4 to 10 years) who did not show any improvement after 3 months of conservative treatment for otitis media with effusion. Control specimens were taken from normal temporal bones at autopsy. All specimens were observed with light and electron microscopy. Histologic degeneration of the tympanic membrane of patients with otitis media with effusion occurred most often in the lamina propria and the submucosal layer. There was an increase in the thickness of the tympanic membrane as a result of edema and fibrosis of the submucosal layer. A decrease in the thickness of the outer and inner fibrous layers occurred in the lamina propria. The histopathologic changes observed in the lamina propria may result in a change in the elastic properties of the tympanic membrane.

Adult↗

PubMed1994

The clinical and pathological features of isolated aortic regurgitation in relation to its etiology.

Chronic isolated aortic regurgitation (AR) caused by degenerative, rheumatic, and Marfan etiologies were compared in a study of 87 patients. There were three hospital deaths in the Marfan group, but none in the rheumatic and degenerative groups. The late postoperative survival rates at 5 and 10 years were 98% and 94%, respectively, in the rheumatic group; 84% and 84%, respectively, in the degenerative group; and 85% and 78%, respectively, in the Marfan group. An analysis of the late complications disclosed a higher incidence of aortic dissection and paravalvular leakage in the degenerative and Marfan groups than in the rheumatic group. In the degenerative group, 4 of the 32 patients developed acute aortic dissection within 3 years following aortic valve replacement. The aortic root diameter in these 4 patients was more than 40 mm at the time of surgery, whereas it was less than 40 mm in the remaining 28 patients. In conclusion, considering the progressive nature of myxomatous degeneration, patients with a severely dilated aortic root diameter should be monitored carefully with echocardiography after surgery.

Adult↗

PubMed1994

Biosynthesis of mucin derived from a 60-kDa precursor protein in the human stomach.

We studied the biosynthesis of mucin in the human stomach using an anti-mucin core peptide monoclonal antibody, 3G12. Human stomach mucosa was labeled with [35S]methionine, and chased for 3 h. An approximately 60-kDa subunit of human gastric mucin precursor protein was detected in the intracellular product. Under nonreducing conditions, dimer, trimer, and tetramer mucin precursor protein (120, 180, 240 kDa) were detected. Treatment with tunicamycin or endo-beta-N-acetylglucosaminidase H had no effect on the 60-kDa subunit and its oligomers. Extracellular products contained only the high molecular weight mucin, and the secretion was not affected by tunicamycin. By treatment with monensin or brefeldin A, the mature mucin was not secreted extracellularly. These findings suggested that a 60-kDa subunit of the mucin precursor protein was biosynthesized into mature mucin after oligomerization to tetramers, and that neither the oligomerization nor the intracellular transport of the mucin in the human stomach was associated with N-glycosylation.

Antibodies, Monoclonal↗

PubMed1994

Biosynthesis and secretion of mucin-related products in Hs746T gastric cancer cells.

We have previously studied the biosynthesis and secretion of mucin in the normal human stomach and reported that the tetramer of the 60-kDa subunit of mucin core protein was synthesized and highly glycosylated, and that only high molecular weight mucin was secreted. In this study, we investigated the mucin-related products of a gastric cancer cell line (Hs746T). Analysis of intracellular and extracellular products immunoprecipitated with an anti-apomucin monoclonal antibody revealed that a 110-kDa protein, the dimer of the 55-kDa subunit, was synthesized and secreted. Tunicamycin treatment inhibited the secretion of the 110-kDa protein. These findings suggest that N-glycosylation may be involved in the secretory mechanism of the mucin-related product.

Adenocarcinoma↗

PubMed1994

Cytokeratin patterns of normal middle ear epithelia in humans, cats, and chinchillas.

We describe the cytokeratin patterns of epithelia from the tympanic orifice, tympanic cavity, and mastoid cavity of humans, cats, and chinchillas, and compare these findings with those of tracheal epithelium and external canal epidermis. Our findings are as follows: 1) middle ear epithelium from all locations demonstrates some type of cytokeratin staining, 2) broad-spectrum cytokeratin antibodies stain epithelia of middle ear cleft, tracheal epithelium, and external canal epidermis in all species, 3) specific cytokeratin antibodies reveal species-related differences in middle ear and tracheal epithelia, 4) middle ear and tracheal epithelia usually have the same pattern, and 5) none of the monospecific cytokeratin antibodies have a positive reaction with external canal epidermis. These findings suggest that the cytokeratin patterns of middle ear epithelium are useful in studying the hyperplastic and metaplastic changes in otitis media; however, caution must be exercised when making interspecies comparisons.

Animals↗

PubMed1994

Surgical indications and treatment of mitral valve disease associated with secundum atrial septal defect with special reference to left ventricular geometry and function.

There are few reports that discussed surgical indications and selection of surgical procedures for secundum atrial septal defect (ASD) associated with mitral valve disease from the viewpoint of left ventricular geometry and function. Our study on 20 patients (6 patients with ASD and MS, 14 patients with ASD and MR) indicated that adult patients with ASD and MS can be treated surgically even when left ventricular end-diastolic volume index (LVEDVI) is as low as 35 ml/m2 (one third of the normal level). Left ventricular wall movement returned to normal one year following surgery. Therefore, rather than myocardial impairment, right ventricular volume load disturbing left ventricular dilatation would be the more likely cause of diminished left ventricular function before surgery in the ASD and MS group. In both groups, two patients who initially had undergone mitral valve replacement died from low output syndrome because of the mismatch between the left ventricular volume and the prosthesis used, whereas there were no deaths in the patients who had mitral valve repair. For this reason, mitral valve repair is the treatment of choice for patients with both secundum ASD and mitral valve disease. When valve replacement is the only alternative, selection of prosthetic valves and maintenance of cardiac output by temporary cardiac pacing are important considerations. A low profile valve with a larger orifice area should be selected to avoid the mismatch between a left ventricular volume and a prosthesis.

Adult↗

PubMed1994

Enhancement in gastric mucosal laminin receptor expression with ulcer healing by sucralfate.

The expression of gastric mucosal laminin receptor during chronic ulcer healing in the presence of sucralfate treatment was investigated. Rats with induced gastric ulcers were treated twice daily for 20 consecutive days either with sucralfate at 100mg/kg or vehicle, and at different stages of treatment used for gastric mucosal laminin receptor quantitation. The assays revealed that accelerated ulcer healing (8 days) in the presence of sucralfate was accompanied by a significant enhancement in the laminin receptor expression. A 2.8-fold increase in the receptor expression over that of controls occurred by the 3rd day of sucralfate treatment, reached a maximum of 3.1-fold increase by the 7th day and remained elevated even after 20 days of treatment. The results attest to the ability of sucralfate to stimulate and promote the events associated with gastric mucosal integrity restoration.

Animals↗

PubMed1994

Criteria to detect mitral prosthetic valve dysfunction via continuous wave Doppler echocardiography.

To detect the diagnostic criteria for mitral prosthetic valve dysfunction, Doppler echocardiography was used to assess valve function following mitral valve replacement in 100 patients [50 with St. Jude Medical (SJM) valves, 50 with Björk-Shiley (B-S) valves]. Mitral valve area (MVA) was calculated by dividing a constant of 220 by pressure half time (PHT) measured via Doppler technique [220/PHT]. The relationship of MVA to left ventricular function, geometric valve area (GVA) of the prosthetic valve, body surface area (BSA), and maximum disc-opening angle (maxOA) were assessed. In the SJM valves, MVA significantly correlated with valve area index (GVA/BSA). The % area index [(MVA/valve area index) x 100] was 100.2 +/- 12.8% on the average and no patients with the SJM valve showed the %area index of less than 73%. There was no incidence of valve dysfunction in the SJM valves. We concluded from these results that if the % area index is less than 73% (mean -2SD), the SJM valve dysfunction should be diagnosed. When the B-S valves were examined, there were 19 patients in whom the maximum opening angle had decreased to under 58 degrees. These patients also had decreased MVA and % area index. Evaluation of the relationship between the maximum opening angle and the % area index revealed that the patients in whom the maximum opening angle had diminished to under 50 degrees had low % area index (of less than 70%).(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

PubMed1994

[Changes of arterial CO2 (PaCO2) and urine output by carbon dioxide insufflation of the peritoneal cavity during laparoscopic cholecystectomy].

We studied on the effects of carbon dioxide insufflation during laparoscopic cholecystectomy on the arterial blood gas analysis and urine output. Intra-abdominal pressure was increased up to either 10cmH2O or 15cmH2O, and we compared the PaCO2 values before and during insufflation. Both increase of PaCO2 and decrease in pH were larger in intra-abdominal pressure of 15cmH2O than 10cmH2O. In the intra-abdominal pressure 15cmH2O group, the increase of PaCO2 by CO2 peritoneal insufflation was significantly larger in operative time of more than 60 minutes group than in less than 60 minutes group, but no significant changes were observed in 10cmH2O group. In the group of obesity index of more than 120, elevation levels of PaCO2 by CO2 insufflation were significant, but in the group of less than 120 no significant elevation were observed. The tendency that the urine output during operation was decreased as increase of intra-abdominal pressure or operative time, but the obesity had no definite effects on urine output. Insufflation of the abdomen with CO2 caused large changes in PaCO2 or pH in the patients with a previous history of major cardiopulmonary disorder. It was shown that the low insufflation pressure and short operative time is good for the maintenance of normal physiological state.

Adult↗

PubMed1994

[The indication of operative procedure for a metastatic spine tumor: a scoring system for the preoperative evaluation of the prognosis].

Since 1987, the operative procedure for a metastatic spine tumor has been decided on the basis of a scoring system for the preoperative evaluation of prognosis. Six parameters which affected the prognosis were employed in this assessment: 1) the general condition, 2) the number of extraspinal bone metastases, 3) the number of metastases in the vertebral body, 4) incidence of metastasis to a major internal organ (lungs, liver, kidneys, and/or brain), 5) the primary site of the cancer, and 6) the severity of spinal cord palsy. Each parameter was scored from 0 (high risk) to 2 (low risk) points. An excisional operation was performed when the total score was above 9 points, while a palliative operation was indicated for those who scored under 5 points. This assessment system was evaluated for 113 cases who had undergone surgery according to this criterion. The survival period of 92.3% of patients who scored less than 6 points was less than 6 months, while that of 78.3% of patients who scored more than 8 points was more than one year. The total score obtained for each patient correlated with the prognosis, and was valuable in predicting prognosis for a lesion at any stage. In conclusion, it was appropriate to indicate the operative procedure for a metastatic spine tumor according to this scoring system.

Adolescent↗

PubMed1994

cDNA cloning of monodehydroascorbate radical reductase from cucumber: a high degree of homology in terms of amino acid sequence between this enzyme and bacterial flavoenzymes.

The FAD-enzyme monodehydroascorbate (MDA) reductase catalyzes the regeneration of ascorbate from the MDA radical using NAD(P)H as the electron donor [Hossain and Asada (1985) J. Biol. Chem. 260: 12920]. We cloned a cDNA of MDA reductase from cucumber seedlings and deduced its entire sequence of amino acid residues. The cDNA library from cucumber seedlings in the expression vector was screened with an antiserum against cucumber MDA reductase. Inserts from three immunoscreened clones hybridized with two oligonucleotide probes designed on the basis of the sequences of two peptide fragments from the cucumber enzyme. The nucleotide sequences of these three clones were determined and the longest one contained an open reading frame of 1,302 bp in length. The molecular mass of the translation product predicted from the open reading frame was 47 kDa, the same as that determined for the purified enzyme. The amino acid sequences determined from fragments of lysyl endopeptidase-digested MDA reductase could be aligned with that deduced from the open reading frame, although substitution of several residues was apparent. Thus, the open reading frame encoded an isozyme of MDA reductase of cucumber different from the purified enzyme. MDA reductase has the FAD- and NAD(P)H-binding domains of flavoproteins but shares only limited homology in terms of amino acid sequence with flavoenzymes from eukaryotes.

Amino Acid Sequence↗

PubMed1993

Different subsets of CNS neurons express different glycosaminoglycan epitopes on large perineuronal proteoglycans.

Proteoglycans are known to occur in the central nervous tissue, but their function is not well understood. We made a biochemical study of four perineuronal antigens on different subsets of rat brain neurons and found that three antigens recognized by antibodies against glycosaminoglycan epitopes were large proteoglycans. Molecular masses estimated by immunoblotting were 700 kDa for 473, 376 and 1B5 antigens and 600 kDa for the 374 antigen. Reactivities of the antigens on immunoblots to monoclonal antibodies (MAbs) 473 and 376 were lost, and that to MAb 1B5 uncovered, after chondroitinase ABC treatment as in the brain sections. The elution profiles from ion-exchange and gel-filtration column chromatography of 473, 376 and 1B5 antigens were quite similar, but those of 374 antigen were slightly different. The immunoaffinity-purified 473 antigen migrated at 700 kDa on sodium dodecylsulfate gel electrophoresis, and chondroitinase ABC treatment decreased the molecular mass to 600 kDa. The 473 antigen was recognized by MAbs 376 and 1B5 although these MAbs also reacted with 473-negative 700 kDa molecules. These results suggest that neuronal subset-specific glycosylation may occur on the 700 kDa proteoglycans, and that the glycosaminoglycan structures may be involved in the pericellular diversity of CNS neurons.

Animals↗

PubMed1993

Major histocompatibility antigen-restricted cytotoxicity in inflammatory bowel disease.

BACKGROUND: The role of cytotoxicity mediated by peripheral blood mononuclear cells for colonic epithelial cells in inflammatory bowel disease (IBD) is still controversial. To clarify it, we studied major histocompatibility antigen (MHC)-restricted T cell-mediated cytotoxicity (CTL). METHODS: Cytotoxicity was measured by 51Cr release from colonic cells after the 6-hour incubation with peripheral blood mononuclear cells in 11 IBD patients (6 with Crohn's disease and 5 with ulcerative colitis). RESULTS: CTL activity (E/T ratio = 200:1 or 100:1) for autologous target cells was significantly increased (22%-40%) in 5 of 6 CD and 4 of 5 UC patients (22%-64%) compared with that for allogeneic target cells. The increase in CTL activity was mainly inhibited by anti-MHC class I and CD8 monoclonal antibodies (50 micrograms/mL), while it was partially inhibited by anti-MHC class II or CD4 antibodies in some patients. Complement-mediated depletion of CD2+ cells also significantly decreased CTL activity. CONCLUSIONS: The results indicate that MHC-restricted T cell cytotoxicity may play a role in mucosal damage in some patients of IBD.

Adolescent↗

PubMed1993

Introduction of functional groups onto the surface of polyethylene for protein immobilization.

Amino and carboxyl groups could be introduced onto the surface of high-density polyethylene film by utilizing graft polymerization of acrylamide and the subsequent Hofmann degradation and alkaline hydrolysis of grafted polyacrylamide. Graft polymerization was carried out by immersing an argon-plasma treated film in an aqueous solution of the monomer, followed by heating after degassing the monomer/film mixture. The surface density of these functional groups could be increased up to 10(-7) mol/cm2. The surfaces having amino and carboxyl groups exhibited positive and negative zeta potentials, respectively, when contacted with KCl aqueous solution. Both of the functional groups introduced onto the polyethylene surface were found to be utilizable for covalent immobilization of protein using carbodiimide for the carboxylic group or mediators such as glutaraldehyde and ethylene glycol diglycidyl ether for the amino group.

Acrylic Resins↗

PubMed1993

Evaluation of clinical lumbar instability using the treadmill.

The physical signs of lumbar instability have not yet been clearly defined. Furthermore, they do not always coincide with the abnormal movement on flexion-extension radiographs. Thus, the treadmill was used to evaluate clinical lumbar instability. The subjects were 82 patients with degenerative lumbar disease aggravated by walking or daily physical activities. The reproduction and provocation of low-back pain or pain in the lower extremities was examined during walking on a treadmill. The responses of the reproduction and provocation of the symptoms were divided into four grades. Fifteen cases were examined again using a treadmill after body casting to determine the indication for fusion surgery. As a result, the responses in the reproduction or provocation test using a treadmill manifest more dominant reflection of clinical symptoms suggesting clinical lumbar instability than the existence of the abnormal movement on flexion-extension radiographs. Reexamination after body casting was useful to determine the indications of fusion operations. The reproduction or provocation test using a treadmill was implied as a possible functional evaluation method of clinical lumbar instability.

Casts, Surgical↗

PubMed1993

Effect of short-term administration of dexamethasone on canine antral gastrin gene expression.

The acute effect of corticosteroids on the release and biosynthesis of gastrin still remains unclear. We report the effect of short-term administration of dexamethasone on the antral gastrin gene expression and gastrin levels in 6 dogs, which were treated with dexamethasone (1.5 mg/kg, i.m. daily) for 3 days. One day after treatment, gastrin mRNA levels increased (152 +/- 14%), but serum and tissue gastrin showed no significant changes. With 2 and 3 days of dexamethasone treatment, gastrin mRNA (2 days: 163 +/- 28%, 3 days: 170 +/- 22%), serum gastrin (2 days: from 94 +/- 12 to 168 +/- 23 pg/ml, 3 days: to 180 +/- 16 pg/ml) and tissue gastrin concentration (2 days: from 480 +/- 32 to 664 +/- 31 pg/mg tissue protein, 3 days: to 708 +/- 45 pg/mg tissue protein) significantly increased. However, gastrin mRNA levels were not increased even after 1 day culture of canine antral tissue with dexamethasone in vitro. These findings suggest that short-term administration of corticosteroid may increase the canine gastrin gene expression in vivo by an unknown pathway.

Animals↗