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Biomedical subjects

S Sano

Publications and source records attributed to S Sano.

At least 235 records · Page 13Linked to original sources
PubMed1984

Thiamine pyrophosphatase and nucleoside diphosphatase in rat brain.

Two types of nucleoside diphosphatase were found in rat brain. One (Type L) had similar properties to those of the liver microsomal enzyme with respect to its isoelectric point, substrate specificity, Km values, optimum pH, activation by ATP and molecular weight. The other (Type B), which separated into multiple forms on isoelectric focusing, had lower Km values and a smaller molecular weight than the Type L enzyme, and was inhibited by ATP. The Type B enzyme catalyzed the hydrolysis of thiamine pyrophosphate as well as those of various nucleoside diphosphates at physiological pH, while Type L showed only nucleoside diphosphatase activity at neutral pH. These findings suggest that the two enzymes play different physiological roles in the brain.

Acid Anhydride Hydrolases↗
PubMed1984

Reduced response of scleroderma fibroblasts to fibroblast growth factor.

Reactivity of scleroderma fibroblasts to lymphoid cell-derived fibroblast growth factor (FGF) was assessed in this study. The fibroblasts from the sclerotic lesion failed to respond to FGF, whereas those from scleroedematous lesions responded equally to normal fibroblast. Response of the fibroblast from sclerotic lesion was also lower than that of the fibroblast from mature scar. Fibroblasts obtained from three different layers of healthy skin, papillary dermis, reticular dermis, and reticular-subcutaneous layer, responded equally to FGF, whereas the fibroblast of reticular dermis from sclerotic lesion failed to respond to FGF. It is suggested that the fibroblast of reticular dermis in scleroderma is variously activated by some unknown factors, so that they do not have enough reserve to respond to further stimuli.

Animals↗
PubMed1984

The cutaneous lymphomas with convoluted nucleus. Analysis of thirty-nine cases.

The clinical and histopathologic findings in thirty-nine cases of cutaneous lymphoma characterized by a proliferation of convoluted cells were reviewed. Thirty patients (77%) presented with erythematous or papular lesions (group I). The remaining nine patients (23%) presented with cutaneous nodules or tumors without erythematous lesions (group III). Histologically, the polymorphous infiltration was the common feature in both groups, but the presence of epidermotropism in group I was contrasted to the absence of epidermotropism in group II disease. The patients in group I had a higher median age (54 years) than those in group II (35 years), and a more favorable 3-year survival rate (44%) than that of the latter (19%). An evolution occasionally occurred from the epidermotropic to the nonepidermotropic form, followed by a poor clinical course. When the skin lesions were nonepidermotropic , irrespective of preceding clinical course, the prognosis was poor.

Adult↗
PubMed1984

pH dependence of the binding constant of a phospholipase A2 from Agkistrodon halys blomhoffii venom to micelles of n-hexadecylphosphorylcholine.

The phospholipase A2 from the venom of A. halys blomhoffii was titrated with micellar n-hexadecylphosphorylcholine (an analog of lysolecithin) by following the tryptophyl fluorescence change at 25 degrees C and ionic strength 0.1. The data were analyzed by assuming that the micellar surface has multiple binding sites for the enzyme and that these sites are identical and mutually independent. The enzyme binding site was found to accommodate a constant number of the substrate (monomer) molecules, N = 10.0 and 6.7 for the apoenzyme and its Ca2+ complex, respectively. The binding constant of the enzyme to the substrate micelle was found to be enhanced by Ca2+ binding to the enzyme. The pH dependence of the binding constant of the apoenzyme to the micelle was well interpreted in terms of pK shifts of two ionizable groups from 5.16 to 5.67 and from 6.45 to 6.6. The pH-dependence curve for the enzyme-Ca2+ complex, which lacked the former transition, was interpreted in terms of the pK shift of a single ionizable group from 5.55 to 5.76. The former ionizable group was assigned as Asp 49, to which Ca2+ ion can coordinate, and the latter as His 48 in the active site. No participation of the alpha-amino group with a pK value of 7.30 was observed. The binding constant of the enzyme to the substrate micelle, Kmic = 0.45-2.3 X 10(6) M-1, was found to be far greater than that to the monomeric substrate, Kmon = 0.2-1.0 X 10(4) M-1. This was interpreted in terms of the presence of an additional weak substrate-binding site in the enzyme molecule.

Binding Sites↗
PubMed1984

Malignant fibrous histiocytoma developing in a burn scar.

A case of malignant fibrous histiocytoma (MFH) developing in a burn scar is presented. The patient was burnt at 3 years of age and 25 years later, a rapidly growing tumour developed in the burn scar. MFH was diagnosed by light and electron microscopy and by an immunoperoxidase method. This complication of a burn scar has not been previously reported.

Adult↗
PubMed1984

Ultrastructural and histochemical analysis of thready material in Pseudoxanthoma elasticum.

The components of the thready material in skin lesions of pseudoxanthoma elasticum, recessive Type I, were analysed. Immunoelectron microscopic observation revealed the presence of fibrinogen in the thready material. Part of the fine fibrils in the thready material was sensitive to collagenase. The thready material was also stained by ruthenium red. These results suggest that the thready material is composed at least of fibrinogen, collagenous protein and glycoprotein.

Adult↗
PubMed1984

Eosinophilic spongiosis in bullous pemphigoid.

Eosinophilic spongiosis is accepted as one of the histopathological features of pemphigus. We found eosinophilic spongiosis in six (24%) of 25 patients in whom erythematous lesions, which were in the vicinity of blisters of bullous pemphigoid, were found. This finding was associated with eosinophilic infiltration of the upper dermis and peripheral eosinophilia.

Aged↗