[Clinical evaluation of bilateral diffuse lung uptake of 67Ga-citrate].
Explore the source record for details and available documents.
Adult↗
Biomedical subjects
S Sakuma
Publications and source records attributed to S Sakuma.
[Intraoperative lumbar injection of Ringer's solution for surgical treatment of chronic subdural hematomas].
A series of 64 patients underwent surgical treatment for chronic subdural hematomas between 1980 and 1989. The procedure consisted of a single burr-hole, irrigation of the hematoma without subdural drainage and lumbar intrathecal injection of Ringer's solution. Irrigation was carried out until the washing was clear and the injection was continued until the brain was in contact with the edge of the burr-hole. Excellent outcome was achieved in 53 patients (82.8% of the total). Two of the patients died, thus making a 3.1% mortality rate. Such complications as tension pneumocephalus and intracerebral hematoma were not observed and no patient had a recurrence.
Adult↗
Effects of Fe2+, Fe3+ and desferrioxamine on paraquat accumulation by rabbit kidney cortex slices.
Effects of Fe2+, Fe3+ and desferrioxamine (DFO) on the accumulation of paraquat (PQ) into rabbit kidney cortical slices were studied. Fe3+ showed dose-dependent inhibition of PQ accumulation. Fe2+ had a more powerful stimulatory effect on the lipid peroxidation of cortical slices than Fe3+, but it did not reduce PQ accumulation. The addition of DFO made the inhibitory effect of Fe3+ on PQ accumulation more pronounced. These results suggest that Fe3+ is able to inhibit the accumulation of PQ independent of its peroxidative action, and that Fe(3+)-desferrioxamine (ferrioxamine) may serve as an antidote for the toxicity of PQ.
Animals↗
[Basic theory of MRA and development of imaging technique].
Explore the source record for details and available documents.
Angiography↗
[Evaluation of 67Ga accumulation around the shoulder joint--comparison with 99mTc-MDP].
67Ga scintigraphy was performed in 865 patients, and in 1078 examinations. 67Ga accumulation was evaluated retrospectively for asymmetric accumulation in the shoulders. Approximately 50% of patients showed no asymmetric distribution of 67Ga in the shoulders. About 40% of patients showed increased accumulation in the right side and about 10% of patients showed increased accumulation in the left side. A change of 67Ga distribution in the shoulder joints was recognized in about 30% of patients during the follow-up study. Reasons for asymmetric distribution of 67Ga in the shoulders were considered to be due to bone metastasis, changes of local blood flow, edema, irradiation, muscle atrophy after cerebral paralysis, and operation. 67Ga scintigraphy showed a broader range and more prominent accumulation in the soft tissues around the shoulder joint than 99mTc-MDP scintigraphy. The accumulation of 99mTc-MDP was recognized to be in the center of the region of 67Ga accumulation. 99mTc-MDP scintigraphy showed more prominently increased accumulation than 67Ga scintigraphy in patients with osseous lesions. Similar factors appeared to have had an influence on the abnormal accumulation of 67Ga and 99mTc-MDP around the shoulder joint.
Adult↗
Effective use of rabbit gastric antral mucosal slices in prostaglandin synthesis and metabolism studies.
Intact slice preparations of rabbit stomach (antral mucosa, corporal mucosa, antral muscle and corporal muscle) were incubated and the released prostaglandins (PGs) were measured by reverse-phase high-performance liquid chromatography using 9-anthryldiazomethane for derivatization. With respect to total PG production, the highest amounts were generated by antral mucosal slices. Antral mucosal slices produced PGE2, 6-keto PGF1 alpha, thromboxane B2, PGF2 alpha and PGD2 (in descending order of magnitude) and possessed a high capacity for producing 13,14-dihydro-15-keto derivatives of both PGE2 and PGF2 alpha. Studies utilizing aspirin, EGTA or Ca2+ revealed that PG release by antral mucosal slices in the present in vitro system reflects a composite of the activities of phospholipase A3, PG cyclooxygenase and PG-metabolizing enzymes. These results show that antral mucosal slices will be useful in physiological and pharmacological studies on PG synthesis and metabolism of the stomach.
Animals↗
[Retrograde balloon catheter dilatation of urethral stenosis--experience in benign prostatic hypertrophy and postoperative urethral stricture].
Retrograde transurethral balloon dilatation of urethral stenosis was performed in five patients: four patients with benign prostatic hypertrophy and one with urethral stricture following open prostatectomy. Significant resolution of symptoms of dysuria was seen in four patients throughout the follow-up period of 12 to 18 months. A balloon diameter of 25 mm was considered to be necessary for prostatic hypertrophy. The unsatisfactory result in one patient with prostatic hypertrophy was believed to be caused by incomplete dilation due to a small balloon diameter. Mild transient hematuria was seen in all cases. Only one patient with postoperative urethral stricture complained of pain during balloon inflation, while other patients with prostatic hypertrophy did not complain of any apparent pain. We conclude that this technique is a safe and effective method of treatment for prostatic hypertrophy and other urethral strictures.
Aged↗
The cell population of somatostatin and growth hormone-releasing factor using quantitative immunohistochemistry in the isolated GH deficient dwarf rat.
We examined the cell population of somatostatin (SS) in the periventricular nucleus (PN) and growth hormone-releasing factor (GRF) in the arcuate nucleus (ARC) between spontaneous dwarf rats (SDRs; gene symbol: dr), which show isolated GH deficiency, and normal rats using avidin-biotin complex (ABC) immunohistochemistry. The total number of SS perikarya per brain weight in the PN of SDRs was 824.8 +/- 49.6 (mean +/- S.E.M., n = 4), whereas that of controls was 1108.5 +/- 50.1 (n = 4). The GRF perikarya per brain weight in the ARC of SDRs numbered 1281.0 +/- 26.0 (n = 7), as compared to 685.4 +/- 64.6 (n = 7) in the controls. The SS perikarya in the PN of SDRs were significantly reduced (P less than 0.05), while the GRF perikarya in the SDRs were significantly increased (P less than 0.01). These results suggest that GH itself acts on SS to positively regulate its secretion and on GRF in a negative regulatory manner.
Animals↗
Thymic stroma-derived T cell growth factor (TSTGF): III. Its ability to promote T cell proliferation without stimulating interleukin 2- or 4-dependent autocrine mechanism.
A thymic stroma-derived cell clone, MRL104.8a produced a T cell growth factor designated as thymic stroma-derived T cell growth factor (TSTGF). This factor that is distinct from previously described T cell growth factors such as interleukin (IL) 2 or 4 was capable of promoting the growth of IL2-dependent, antigen-specific helper T cell clones. While such growth promotion was induced without requirement of the relevant antigen and exogenous IL2, we further investigated whether it depended on activation of an IL2- or IL4-dependent autocrine mechanism. Helper T cell clones, 8-E and 8-5, were able to proliferate in response to stimulation with either antigen or TSTGF. 8-E and 8-5 produced IL2 and IL4, respectively, in cultures following antigenic stimulation, whereas neither IL2 nor IL4 activity was detected in cultures during TSTGF-induced proliferation. The proliferation of these helper T cell clones by antigenic stimulation was almost completely inhibited when anti-IL2 receptor or anti-IL4 antibody was added to the cultures. The addition of cyclosporin A (CsA) to cultures of 8-E and 8-5 clones together with antigen also resulted in the complete inhibition of cellular proliferation in association with the suppression of IL2 and IL4 production. In contrast, TSTGF-induced proliferation was not affected by addition of either type of antibody or CsA. These results indicate that TSTGF is a novel T cell growth factor that can exert its own growth-promoting effect without depending on an IL2- or IL4-operating autocrine mechanism.
Animals↗
Herpes simplex virus type 1 infection in mice with severe combined immunodeficiency (SCID).
Explore the source record for details and available documents.
Animals↗
Partial trisomy of the distal segment 14q.
The case of a male infant with duplication of the distal segment of chromosome 14q is described. There was an extra chromosomal segment at the distal end of the long arm of chromosome 17. Banding techniques suggested that the extra segment might be the distal segment of chromosome 14q. DNA analysis using probes from distal 14q as well as from other parts of the genome confirmed that the extra segment consisted of the distal part of 14q. Both the proband's parents and his elder sister had normal karyotypes.
Abnormalities, Multiple↗
Evaluation of primary lung carcinoma using technetium 99m-hexamethylpropylene amine oxime: preliminary clinical experience.
99mTc-labelled hexamethylpropylene amine oxime (HMPAO) is a lipophilic compound with a neutral charge which reflects tumor blood flow and has been previously investigated to estimate brain blood flow. In this study, we attempted to use 99mTc-HMPAO for the evaluation of 18 patients with histologically proven primary lung carcinoma. The eight surgical specimens revealed that viable carcinoma cells were present when the 99mTc-HMPAO accumulated in the tumor, however, extensive tumor necrosis was observed when defective or ring like uptake was seen in the tumor. Qualitative study revealed that when perfusion defects were observed corresponding to the tumor, the possibility of squamous cell carcinoma or large cell carcinoma was high instead of adenocarcinoma. Quantitative analysis revealed that the uptake ratio was statistically different between adenocarcinoma (1.6 +/- 0.1) and squamous cell carcinoma (1.2 +/- 0.4) (P less than 0.05), between squamous cell carcinoma and large cell carcinoma (0.9 +/- 0.1) (P less than 0.05), and also between adenocarcinoma and large cell carcinoma (P less than 0.01). In conclusion, 99mTc-HMPAO may be useful for the evaluation of patients with primary lung carcinoma.
Adenocarcinoma↗
Characterization of glycoprotein C-negative mutants of herpes simplex virus type 1 isolated from a patient with keratitis.
Recently three strains of herpes simplex virus type 1 (HSV-1), which did not react with Micro Trak Herpes (Syva Co.), were isolated by us from a patient with recurrent herpetic keratitis. In this study we characterized these strains of HSV-1 and found them to be HSV-1 gC- mutants which are very rare isolates from humans. The properties of the HSV-1 strains regarding plaque morphology on Vero cells and chick embryo fibroblasts and viral DNA analysis were the same as those of the usual HSV-1 strains. An immunofluorescence study using anti-gC-1 monoclonal antibody and SDS-PAGE analysis of radiolabeled viral glycoproteins showed that these strains are deficient in gC-1. They were virulent for mice and sensitive to acyclovir and bromovinyldeoxyuridine. Furthermore the infectivity of the strains was inactivated by complement though the phenomenon was not observed in the usual HSV-1 strains. This finding suggests that protection from damages by complement is an important function of gC. In keratitis the effects of complement are thought to be minimal because of the scanty blood supply and this may be the reason why these strains were isolated from the cornea.
Adult↗
Two-dimensional echocardiography and magnetic resonance imaging in diagnosis of idiopathic dilation of the right atrium.
Explore the source record for details and available documents.
Adult↗
Effects of metal ions on 15-hydroxy prostaglandin dehydrogenase activity in rabbit kidney cortex.
The effects of Cu2+, Fe2+ and Zn2+ on 15-hydroxy prostaglandin dehydrogenase activity in rabbit kidney cortex were examined. Cu2+ and Zn2+ (0.05-0.5 mM) inhibited the activity of this enzyme in a dose-dependent manner. The concentration required for 50% inhibition was approximately 0.1 mM for Cu2+ and 0.15 mM for Zn2+. The inhibition by both metals was uncompetitive and non-competitive with regard to NAD+ and prostaglandin E2, respectively, indicating that the mechanisms of the inhibition on the enzyme of both metals may be the same. Fe2+ had no effect on the activity of this enzyme. These results suggest that Cu2+ and Zn2+ have the potential to modulate the catabolism of prostaglandins by the kidney cortex.
Animals↗
Role of fast data acquisition method with Tc-99m HMPAO brain SPECT in patients with acute stroke.
The first data acquisition method for Tc-99m hexamethylpropyleneamineoxime (Tc-99m HMPAO) brain SPECT was used in two patients with acute stroke 5 and 8 hours after the event. In one patient Tc-99m HMPAO fast SPECT showed perfusion defects before the same was manifested on CT. In the other patient, Tc-99m HMPAO fast SPECT showed larger defects than the edema seen on CT. The Tc-99m HMPAO fast SPECT might be feasible for the evaluation of acute stroke patients. Since the time required for fast SPECT is much shorter than usual SPECT, it appears to be more suitable for some patients with acute stroke who are brought to the hospital in a very critical condition.
Aged↗
Potent inhibition of prostaglandin inactivation in rabbit gastric antral mucosal slices by selenium ions in-vitro.
The effect of selenium ions on prostaglandin (PG) catabolism and synthesis in rabbit gastric antral mucosal slices has been examined. Selenium ions had a potent inhibitory effect on the inactivation process for PGE2 and PGF2 alpha. Simultaneously, the levels of PGE2 and PGF2 alpha were increased. These results suggest that selenium ions have the potential to increase the levels of biologically active PGs in gastric mucosa by preventing their inactivation and that this effect may represent some pharmacological action of selenium ions.
Animals↗
Clinical evaluation of irreversible image compression: analysis of chest imaging with computed radiography.
To implement a picture archiving and communication system, clinical evaluation of irreversible image compression with a newly developed modified two-dimensional discrete cosine transform (DCT) and bit-allocation technique was performed for chest images with computed radiography (CR). CR images were observed on a cathode-ray-tube monitor in a 1,024 X 1,536 matrix. One original and five reconstructed versions of the same images with compression ratios of 3:1, 6:1, 13:1, 19:1, and 31:1 were ranked according to quality. Test images with higher spatial frequency were ranked better than those with lower spatial frequency and the acceptable upper limit of the compression ratio was 19:1. In studies of receiver operating characteristics for scoring the presence or absence of nodules and linear shadows, the images with a compression ratio of 25:1 showed a statistical difference as compared with the other images with a compression ratio of 20:1 or less. Both studies show that plain CR chest images with a compression ratio of 10:1 are acceptable and, with use of an improved DCT technique, the upper limit of the compression ratio is 20:1.
Humans↗