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Biomedical subjects

S Sakata

Publications and source records attributed to S Sakata.

At least 235 records · Page 13Linked to original sources
PubMed1987

A case of systemic lupus erythematosus (SLE) and Sjögren's syndrome associated with anti-T3 autoantibodies.

A case of systemic lupus erythematosus (SLE) associated with Sjögren's syndrome had extremely low serum triiodothyronine (T3) with normal levels of serum thyroxine (T4) measured by single antibody radioimmunoassays (RIAs) and thyroid stimulating hormone (TSH) during steroid treatment. Measurement of serum T3 and T4 with double antibody RIAs showed unusually high T3 and normal T4 concentrations. Examination of her serum revealed the presence of IgG class anti-T3 autoantibodies whose Scatchard plot was analyzed in two components; one with a higher associate constant (8.6 X 10(8)M-1) and a lower binding capacity (5.6 X 10(-7) mol/ml serum); the other a lower associate constant (3.5 X 10(7)M-1) and a higher binding capacity (2.1 X 10(-6) mol/ml serum). Antithyroglobulin (Tg) autoantibody has been positive throughout the seven year observation period. A significant positive correlation between titers of anti-Tg autoantibodies indicated that the antigen of anti-T3 antibodies in the patient could be T3 containing antigenic site(s) on the Tg molecule.

Adult↗
PubMed1987

Dynamics of plasma lipoproteins and lipids during double filtration plasmapheresis (DEP).

Plasma lipids and lipoproteins obtained from different places of the circuit of double filtration plasmapheresis (DFP) were measured and effect of DFP therapy on removal of them was examined. When 2A was used as a second filter, 69.8%, 52.4%, 63.0%, 58.0%, 60.8%, 59.2% and 63.9%, respectively of beta-lipoprotein, cholesterol, triglyceride, high density lipoproteins (HDL), phospholipids, free cholesterol and lipoprotein (a) (Lp(a)) were removed from the patient's plasma. When 4A was used as a second filter, 69.0%, 56.8%, 53.2%, 45.4%, 56.0%, 50.9% and 51.7%, respectively of beta-lipoprotein, cholesterol, triglyceride, HDL, phospholipids, free cholesterol and Lp(a) were removed from the patient's plasma. In contrast, concentrations of free fatty acids (FFA) after DFP therapy using filter 4A and 2A increased to 222.8% and 256.4%, respectively. Thus, it was shown that except for FFA, DFP therapy using either 2A or 4A as a second filter is effective in reducing concentrations of plasma lipid and lipoproteins.

Adolescent↗
PubMed1987

Two cases of systemic lupus erythematosus associated with hyperthyroidism.

We have experienced two cases (Case 1: 21-year-old female, Case 2: 26-year-old female) of systemic lupus erythematosus (SLE) associated with hyperthyroidism. Case 1 had been treated with methimazole (MMI) and betamethasone for approximately two years. Although thyroid function improved with the treatment, laboratory data of SLE deteriorated. She was successfully treated with betamethasone alone. Case 2, who had severe side effect (severe hemorrhage due to gastric ulcer) during prednisolone treatment for SLE, was found to have an additional hyperthyroidism. She was treated with intermittent prednisolone administration alone. Physical findings as well as laboratory data of both SLE and hyperthyroidism improved by the therapy.

Adult↗
PubMed1987

Effect of anti-insulin antibodies on glycemic control in insulin treated diabetic patients.

In order to examine the effect of anti-insulin antibodies on serum insulin concentration and glycemic control, sera from 49 insulin-treated diabetic patients (20 males and 29 females) were examined for their titer of anti-insulin antibodies, and concentrations of total insulin (TIRI), free insulin (FIRI) and hemoglobin A1c. Titer of anti-insulin antibodies correlated only with TIRI (r = 0.72, p less than 0.001) and did not correlate with duration of insulin treatment, FIRI concentration, daily dose of insulin, or HbA1c concentration. These results indicate that elevated titer of anti-insulin antibodies in patients under insulin therapy, (1) increases total serum insulin concentration but does not affect serum free insulin level, and (2) has little effect, if any, on glycemic control and the required daily dose of insulin.

Adult↗
PubMed1987

[Scoring system for the evaluation of results of radiation therapy].

The results of radiation therapy have been evaluated on the basis of local control of the tumor and reaction of the normal tissues. A scoring system, consisting five steps, was proposed to document the response of the tumor as well as the normal tissue reaction in order to evaluate the effects of irradiation. The results of the studies showed that the scoring system was of value to estimate the response of the tumor and the reaction of the normal tissues developed following irradiation. The radiation tolerance of the cutaneous tissue for fast neurons and for 70 MeV protons was expressed as follows. NSD (n, 30 MeV d-Be) = TD N-0.11 T-0.11 (1) NSD (p, 70 MeV) = TD N-0.20 T-0.11 (2) As to the radiation tolerance of the lung, studies are required to determine the optimal scoring system.

Evaluation Studies as Topic↗
PubMed1987

Plasma erythropoietin assay by a fetal mouse liver cell culture method with special reference to effective elimination of erythroid colony inhibitor(s) in plasma.

Methods for the elimination of an inhibitor(s) of erythroid colony formation from plasma were examined in an attempt to measure genuine plasma erythropoietin (Epo) activities with an erythroid colony-forming assay using fetal mouse liver cells. Acid-boiling-chloroform (ABC) treatment was concluded to be the best method because the plasma thus treated stimulated colony formation most and contained the least protein. The dose-response curve for the plasma was parallel to that for the standard Epo preparation. The "erythroid colony-stimulating activity" in the plasma was completely additive to that in the standard Epo, and appeared to be a relatively heat-stable and acid protein with an isoelectric point lower than 5.0. These results suggest that the activity in the plasma is identical to that in the standard Epo. Stability of the plasma Epo activity was dependent on storage temperature and enhanced by adding 1% bovine serum albumin (BSA). Average Epo titers for normal adult, full-term cord, and murine plasmas, all ABC-treated and with 1% BSA added, were 192.4, 184.5, and 150.6 mU/ml, respectively. These values were much higher than those measured by the in vivo standard polycythemic mouse assay.

Animals↗
PubMed1987

Factors affecting general job satisfaction of caregivers in nursing homes.

The important factors affecting general job satisfaction of caregivers in nursing homes in Tokyo were analyzed. The general job satisfaction of caregivers proved to be strongly correlated with such factors as frequency of night shifts, relationship with supervisors and length of employment, feeling of self-esteem and motive for taking the present job, satisfaction with work itself and tension and/or frustration experienced in working with the aged, and education. Such factors as cognition of the necessity of the aged services and knowledge of ageing and old people did not have any great impact on general job satisfaction of caregivers. These findings suggest the necessity of improving work conditions and human relations in workplace, the importance of attitudes and feelings of caregivers when hiring them, and the necessity of the adequate cognition and knowledge in working with the aged in nursing homes.

Adult↗
PubMed1987

Prognostic significance of pancreatic tumor regression after intraoperative radiotherapy.

Tumor volume measurement using tumor-margin clipping radiography was performed for estimating tumor regression of eight pancreatic adenocarcinomas treated with intraoperative electron irradiation. Half of the tumors regressed exponentially from the first day of treatment, but the other half increased slightly within the first week, after which volume reduction occurred. The volume-halving time was calculated from the regression curve. A wide distribution of volume-halving times as well as initial tumor volumes was seen in all patients. No correlation was found between these variables, but the former correlated well with survival time (r2 = 0.80). This well-known technique may offer a chance to foresee the prognosis of the disease by determining the volume-halving time.

Adenocarcinoma↗
PubMed1987

[Volume effect models in radiation therapy].

In radiation therapy, it is clearly recognized that the isoeffect dose of normal tissue decreases with the increase of the area or the volume of tissue irradiated, so that a marked change in therapeutic ratio occurs when large tumor is treated. This relationship has been called "volume effect" and several models such as power law relationship, volume dose histogram and integral probability have been developed to quantify the effect. Although the delivery of these models are based on clinical data, there is a wide variation between models and each model can be applied under restricted conditions. In order to develop reliable volume effect model theoretically and empirically, further studies with enough and suitable clinical data will be required.

Dose-Response Relationship, Radiation↗
PubMed1986

Human haptoglobin binds to human myoglobin.

Human myoglobin, obtained from human heart, was purified to homogeneity by salt precipitation, crystallization and ion-exchange chromatography. Trace contamination by haemoglobin, if any, was removed by repeated adsorption on an immunoadsorbent of anti-haemoglobin antibodies. The interaction between human haptoglobin and human myoglobin was investigated by a solid-phase radioimmunoassay. Myoglobin adsorbents bound 125I-labelled haptoglobin in a specific manner. Linear Scatchard plots of the data indicate that human myoglobin has only one binding site for haptoglobin in terms of the binding affinity (Ka = 8.5 X 10(6) M-1). These results suggest that haptoglobin not only binds haemoglobin but also binds human myoglobin, although with an affinity that is much lower than that of haemoglobin. The physiological significance of this interaction is discussed.

Adsorption↗
PubMed1986

Macroamylasemia associated with thyroid cancer, elevated serum thyroxine-binding globulin (TBG), chronic pancreatitis and gastrointestinal polyposis.

A case of macroamylasemia in a 78 year-old female, associated with papillary adenocarcinoma of the thyroid and elevated serum thyroxine-binding globulin was described. Serum amylase of the patient was very high despite a normal level of serum lipase. Her serum amylase was characterized as macroamylase on the basis of electrophoresis and elution profile of gel chromatography. Serum precipitation response using chain-specific rabbit anti-human IgG, IgA and IgM antisera disclosed that her macroamylase was mainly composed of immune complexes between amylase and IgA class anti-amylase antibodies. Both the pathogenesis and clinical significance of the association of these disorders in this patient were discussed.

Adenocarcinoma, Papillary↗
PubMed1986

Absence of effects on platelet membrane fluidity by antibiotics in ESR spectra.

The effect of several antibiotics on rat platelet membrane fluidity was examined by ESR spectrometry using 5-doxyl stearate as the spin probe. Latamoxef, cefamandole, cefotaxime and carbenicillin had negligible effects on the order parameters, the indices of membrane fluidity, of platelet-rich plasma or washed platelets at high concentrations that would inhibit ADP-induced rat platelet aggregation in vitro. A further in vivo study with one of these antibiotics, latamoxef, showed no significant effect on the fluidity based on ESR data.

Adenosine Diphosphate↗