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Biomedical subjects

S Sakaguchi

Publications and source records attributed to S Sakaguchi.

At least 271 records · Page 15Linked to original sources
PubMed1982

Therapeutic effect of ticlopidine, a new inhibitor of platelet aggregation, on chronic arterial occlusive diseases, a double-blind study versus placebo.

The clinical efficacy of ticlopidine, a platelet aggregation inhibitor, was evaluated in a double-blind placebo-controlled study on 193 patients with ischemic ulcers due to chronic arterial occlusion. In the group treated with ticlopidine, significantly better efficacy was noted over the placebo group in terms of judgment on overall improvement by doctors in charge as well as by the committee assessing with color slide films. The drug was particularly effective for the treatment of patients with occlusion in the arteries on the distal side and for the ischemic ulcer with relatively small size. In the group with ticlopidine, granulation was significantly improved compared to the placebo group. Those patients who had pain due to arterial occlusion on the distal side were significantly improved by this agent. The mean diameters of ulcers were clearly reduced. Therefore, this agent could possibly be claimed as an effective remedy against the main symptoms of chronic arterial occlusion. The frequencies of side effects and abnormalities in laboratory tests were comparable in the group treated with ticlopidine to those of the group with placebo. It was, therefore, concluded that this agent has an outstanding clinical value as a conservative treatment in chronic arterial occlusion.

Arterial Occlusive Diseases↗
PubMed1981

Postoperative assessment of arterial reconstructive surgery using Doppler flow detection.

The ankle systolic pressure was measured by the Doppler flow detector during the postoperative course after arterial reconstructive surgery in 61 limbs. The ankle systolic pressure was expressed as a pressure index (PI). There were 2 patterns in the recovery of PI after successful reconstructions. In one of these patterns, PI returned to the normal level within 24 hours. The other group represented a delayed recovery of PI. A comparative study between these 2 groups indicated that the delay in the recovery of PI may be chiefly related to a poor distal run-off. There was no relationship between the recovery pattern of PI and the late patency of reconstructed arteries. On the other hand, follow-up examination by means of the ankle pressure measurement was useful to detect new occurrences of stenotic lesions in the postoperative period.

Adolescent↗
PubMed1981

Lipid peroxide formation and membrane damage in endotoxin-poisoned mice.

Lipid peroxide formation and plasma membrane damage in mouse liver following the administration of Salmonella endotoxin were examined. The liver lipoperoxide level was markedly elevated in animals given endotoxin compared with that in the controls, and returned to its normal range after 2 days. On the other hand, superoxide dismutase activity was decreased by 18--48 hr after endotoxin injection, thereafter tending to increase. Glutathione reductase and glutathione peroxidase activities declined in the liver 18 hr after the injection. The endotoxin resulted in much lower lipoperoxide formation in the livers of tolerant mice than in those of the poisoned mice. The lipoperoxide level in endotoxin-poisoned mice after the administration of alpha-tocopherol was lower than that in the controls, and alpha-tocopherol administration prevented completely the membrane protein damage that arose from endotoxin challenge. After glutathione administration the membranes of the poisoned mice also returned to almost the normal disk electrophoretic profile. These results suggest that lipid peroxide formation in the liver plasma membrane caused by free radicals might occur in a tissue ischemic state in endotoxicosis.

Adenosine Triphosphate↗
PubMed1981

Effect of alpha-Tocopherol on endotoxicosis.

The effect of alpha-tocopherol in endotoxicosis was studied. The alpha-tocopherol level significantly decreased in mouse liver 18 hr after endotoxin administration, thereafter tending to increase to approach the normal range. In endotoxin-tolerant mouse liver, the lipid peroxide level was reduced to less than half of that in nontolerant animals following endotoxin challenge. The liver lipid peroxide level and serum lactate dehydrogenase or acid phosphatase leakage were studied in mice fed a vitamin E-deficient (ED) diet and a vitamin E-supplemented (ES) diet for 40 days. ED mouse liver exhibited a higher formation of lipid peroxide after endotoxin was given while there was a markedly lower level in ES mouse liver. There was significantly more serum lactate dehydrogenase or acid phosphatase leakage in ED mice than in ES mice after endotoxin administration. There was about a 25% decrease in liver superoxide dismutase (SOD) activity in endotoxin-poisoned mice fed both the normal and the ED diets, while the activity was at a higher level in ES-fed mice. These results suggest that alpha-tocopherol may be helpful in preventing membrane instability in endotoxin poisoning.

Acid Phosphatase↗
PubMed1981

Earlier appearance of murine mammary tumor virus-associated antigens in duct-alveolus nodules induced by transplantation of fetal salivary mesenchyme into C3H mouse mammary glands.

Mammary epithelium of adult C3H mice in contact with fetal salivary mesenchyme took morphogenetically a salivary gland-like pattern of growth and developed a hyperplastic nodular mass at the site of transplantation. When murine mammary tumor virus-bearing C3H mice were treated in this way, early development and high incidence of mammary cancer were observed from these nodules compared to unstimulated mammary glands of the same host. Since tumor development, the viral antigens in these hyperplastic nodules were examined by means of the immunofluorescence technique. As early as 2 weeks after mesenchyme transplantation, the antigens were observed in the ductular or alveolar structure of these nodules, while surrounding normal tissues were not stained. Positive fluorescence was also observed in the mammary tumors that developed from these stimulated nodules. These results strongly suggested the involvement of murine mammary tumor virus in the early development of mammary cancer induced by fetal mesenchyme transplantation.

Animals↗
PubMed1980

Metabolic aspects in mice administered live Salmonella typhimurium.

The influence of intraperitoneal inoculation of live Salmonella typhimurium on carbohydrate and lipid metabolisms in mice was investigated at doses of 9.2 x 10(7) cells, 1.9 x 10(8) cells, and 3.8 x 10(8) cells. The hepatic glycogen content in mice at 18 hr after the inoculation decreased in inverse proportion to an increase in the injection dose. The activities of hepatic phosphorylase and G-6-Pase increased significantly after 2 hr, but after 18 hr the levels of both enzyme activities, especially G-6-Pase, declined in inverse proportion to an increase in dose of viable cells administered to the mice. The levels of serum glucose and free fatty acids (FFA) in mice markedly decreased at doses of 1.9 x 10(8) and 3.8 x 10(8) cells after a transient rise at early stage (1 hr) after the injection. Marked hypertriglyceridemia was seen in infected mice. However, the activity in serum lipoprotein lipase (LPL) was reduced by an increase in the injection dose. The effect of intraperitoneal administration of viable cells on the serum triglyceride level was prevented in mice immunized with S. typhimurium endotoxin or administered with the anti-endotoxin serum. These results indicate that hypertriglyceridemia mainly results from the action of endotoxin in the pathogen. Serum lactate dehydrogenase (LDH) activity markedly increased at the dose of 3.8 x 10(8) cells within 8--16 hr, and the infected mice exhibited a leakage of isozymes LDH-3 and 5 in the serum 16 hr post-inoculation.

Animals↗