Search PubMed⌕ Search

Biomedical subjects

S Rosenthal

Publications and source records attributed to S Rosenthal.

At least 109 records · Page 6Linked to original sources
PubMed1981

Criteria of maturity and age of red blood cells.

Any work on red blood cells requires information about the characteristics of a cell population. Using buoyant density separation of red blood cells (RBC) in a dextran medium during a bleeding anemia of rabbits cell-physiological, ribosomal, mitochondrial and cytoplasmic criteria were estimated in order to select parameters useful for the characterization of a given RBC population according to its developmental stages. Useful criteria for an unknown overall RBC population are reticulocyte count and aspartate amino transferase, or glucose-6-phosphate dehydrogenase activity or creatine concentration. Useful additional criteria for reticulocytes are the mean cellular volume and the RNA concentration.

Animals↗
PubMed1980

Cloning of carp preproinsulin cDNA in the bacterial plasmid pBR322.

The successful cloning of recombinants between cDNA from fractionated poly(A)+-RNA of Brockmann bodies of the carp and the plasmid pBR322 in Escherichia coli chi 1776 is reported. One of the recombinant clones has been identified as a preproinsulin-cDNA recombinant by the hybrid-arrest translation assay. Recombination was at the PstI site of pBR322; reconstitution of this site was by 3'-tailing of the vector with dGn. The transformants were screened by in situ hybridization with kinase-labeled poly(A)+-RNA sedimenting at 9S from Brockmann bodies. Restriction analysis was performed on 26 of the strongly hybridizing clones to estimate the size of the inserted cDNA. Six of the recombinants studied contain inserts of a size approximating to full length 9S preproinsulin mRNA. The hybrid-arrest translation assay on selected clones identified one as a recombinant containing the preproinsulin cDNA sequence.

Animals↗
PubMed1980

Holocaust effect in the third generation: child of another time.

This case report aims to make psychiatrists aware of the need for a "psychohistorical" approach to the diagnosis and treatment of patients who are the third generation of the holocaust, and identify the nature of the multigenerational processes within such a family.

Child↗
PubMed1980

Medical therapy of Prinzmetal's variant angina.

Medical therapy for Prinzmetal's variant angina has been treatment of the acute attack with sublingual nitroglycerin. Prophylactic therapy has been more difficult, utilizing long-acting vasodilators that are limited because of their short half-life and side effects when therapeutic doses are used. Alpha-adrenergic blockade has been effective in some patients but is frequently associated with intolerable side effects or apparent development of tolerance to the drug. Preliminary experience from a randomized double-blind trial of diltiazem, a new calcium antagonist, has demonstrated a 90% reduction in pain episodes, with many patients becoming pain-free on the 240-mg daily dose. These data and the lack of adverse side effects demonstrate a dramatically effective therapy for patients with coronary artery spasm.

Angina Pectoris↗
PubMed1980

[Improved test for the detection of reverse transcriptase of bovine leukosis virus].

The revertase test with exogenous matrix, poly-(rA) . oligo-(dT), for the detection of bovine leukemia virus from crude virus sediments was standardised and miniaturised. An amount of 10 ml of cell culture supernatant of short-term cultured lymphocytes (5 . 10(6) cells/ml) is quite sufficient for testing one cattle sample. The lower sensitivity limit of the test was found to be 10(6) virus particles. The test is properly reproducible, within tolerance limits of +/- 20--30 per cent, provided that optimum lysis conditions be maintained (0.01 per cent triton X-100, 20 minutes, 0--4 degrees C incubation) and under the condition that the protein quantity in 100 microliter test solution does not exceed the threshold of 3--15 micrograms. The specificity of the test is based on the use of free viruses from cell culture supernatant, the optimum temperature of the revertase reaction at 25 degrees C, which actually deviates from that for cellular DNA polymerases, that is 37 degrees C, and magnesium ion concentration which has to be optimum for bovine leucosis virus revertase. Two-hundred heads of cattle, differing by haematological status, were examined, and 56 per cent of them were, clearly, virus producers, among them 95 per cent of all animals with positively established leucosis and 36 per cent of the haematologically intact animals. Examinations of individuals have shown that in repetitive checks, carried out in intervals between two months and one week, the revertase activities varied by something between 0.5 and two magnitudes.

Animals↗
PubMed1980

Contamination in McCarey-Kaufman stored keratoplasty donor material.

One hundred consecutive donor corneas which had been preserved in McCarey-Kaufman medium and used in actual keratoplasties were studied. The residual medium and button remnants were cultured for bacterial and fungal growth. Coagulase-positive staphylococci were recovered in three cases, coagulase-negative staphylococcus in one and Rhodotorula sp. in another; no associated postoperative infections were reported, although one patient was regrafted 48 hours after the initial keratoplasty for "graft decompensation"--the initial button was not submitted for reculture. One additional specimen was processed. A cracked vial with clear contained fluid was held for culture; Pseudomonas cepacia was grown from the fluid. Other samples in undamaged vials from the same lot did not show contamination.

Anti-Bacterial Agents↗
PubMed1978

[Separation of bone marrow cells of normal and anemic rabbits using isopycnic dextran density gradient].

Bone-marrow cells of normal and anemic (phenylhydrazine) rabbits were separated in the isopyknotic continuous 50-ml dextran density gradient. The separated cells were divided into 3 groups according the benzidine-peroxide-Giemsa staining after Brosook: nucleated, nonerythroid (A), nucleated, erythroid (B), and nonnucleated, erythroid (C). The highest proportion of erythroid, nucleated cells was found in fractions of medium density: in normal animals 20%, in anemic ones 45%. The group B/A ratio in the unseparated suspension is 0.7 in normal animals and 1.7 in anemic ones; and in the most favourable density fraction 0.3 and 1.9 respectively. Thus an unimportant accumulation of erythroid bone-marrow cells of rabbit is observed. In constrast, an accumulation of nucleated leukocytes of 80--90% can be reached in the low-density fractions.

Anemia↗
PubMed1977

Erythroblastic transformation of chronic granulocytic leukemia.

Erythroblastic transformation of chronic granulocytic leukemia was found in seven of 67 unselected patients with blast crisis. This morphologic picture of erythroblastic transformation was indistinguishable from that in erythroleukemia or Di Guglielmo's syndrome. The median survival of the patients with erythroblastic transformation was two months, considerably less than the four-month median survival in the entire series of 67 patients. Only two brief partial remissions were obtained with combination chemotherapy. The causes of death were primarily hemorrhage and infection, related to thrombocytopenia and neutropenia. In this regard, the patients with erythroblastic transformation resembled all the patients with blast crisis and patients with acute leukemia in general. The erythroblastic transformation seems to represent a morphologic variant of chronic granulocytic leukemia blast crisis, without apparent prognostic or therapeutic implications.

Adult↗
PubMed1977

Blast crisis of chronic granulocytic leukemia. Morphologic variants and therapeutic implications.

Chronic granulocytic leukemia (CGL) terminates in a disease similar to acute leukemia. Except for a study indicating an increased response rate to the drugs, vincristine and prednisone, therapy of this terminal phase has been universally disappointing. We have studied the bone marrows and clinical courses of 67 patients in the terminal phase of CGL to discern if any parameters were associated with an increased response rate or survival. The results of this study indicate that patients who have a lymphoblastic morphology or hypodiploid cytogenetics in the terminal phase respond better to treatment with the combination of vincristine and prednisone than those with myeloblastic morphology or hyperdiploid cytogenetics. Response rate and survival are significantly increased in those with lymphoblastic morphology. Recognition of the heterogeneity of the terminal phase of CGL may dictate specific therapeutic modalities.

Adult↗
PubMed1977

Basophilic chronic granulocytic leukaemia with hyperhistaminaemia.

Two patients with typical Philadelphia chromosome positive chronic granulocytic leukaemia (CGL) developed an accelerated phase of the disease characterized by an increase white blood cell count and marked basophilia in the bone marrow and peripheral blood. Histamine levels were extremely high in both patients. Hyperhistaminaemia was manifested as wheezing, urticaria, diarrhoea, and pruritus in one patient and as peptic ulcer disease and peripheral oedema in both patients. In one case, gastric acid studies revealed a very high basal to stimulated ratio (BAO/MAO). Treatment with the investigational agent metiamide, an H2 receptor histamine antagonist, resulted in marked improvement in symptoms and reduction in gastric acid output. Extreme basophilia in CGL may be associated with hyperhistaminaemia, and manifestations of both the H1 and H2 type may occur.

Adult↗
PubMed1977

Abnormalities in taste preference in hypothyroid rats.

Rats made hypothyroid by administration of radioactive iodine and age-matched controls were individually caged and offered a choice between either water or varying concentraions of sweet (sucrose), bitter (quinine sulfate), salty (NaCl) or sour HCl solutions to drink ad libitum for 48 h periods. Comparative measurements were made of the volume of test solution consumed to that of total volume consumed and were expressed as taste preferences. Throughout a wide range of concentrations, taste preferences for sucrose were significantly lower (P less than 0.001) and those for quinine sulfate and NaCl significantly higher (P less than 0.001) in hypothyroid animals than in controls. Taste preferences for HCl were generally similar in both groups. Daily intraperitoneal injections of thyroxine, 10 mug/100 g body wt. to hypothyroid rats for 18-24 days eliminated completely the difference between hypothyroid and control rats in taste preference for quinine sulfate. These studies show that significant and reversible changes in taste preferences occur in rats rendered hypothyroid with radioactive iodine.

Animals↗