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Biomedical subjects

S Rosendal

Publications and source records attributed to S Rosendal.

112 records · Page 7Linked to original sources
PubMedDate not supplied

Colonization of the intestine of turkey embryos exposed to Mycoplasma iowae.

Eight-day-old turkey embryos were inoculated into the yolk sac with 3 X 10(5) colony-forming units of Mycoplasma iowae strain D112 in order to study the growth-depressing effect, the histopathological changes, and colonization of the intestinal tract. The embryo: egg weight ratio was significantly lower in the inoculated eggs than in controls. Histologically, there were infiltrations in parenchymatous organs and chorioallantoic membranes with heterophilic granulocytes. M. Iowae was demonstrated on the intestinal mucosa by antibody fluorescent microscopy and by transmission electron microscopy. Attaching mycoplasmas had a distinct morphology; the segment in contact with enterocytes was cone-shaped and had finely granulated cytoplasma which was abruptly separated from the distal coarsely granulated area. We conclude that M. iowae has a predilection for the intestinal tract of avian hosts.

Animals↗
PubMedDate not supplied

Oral challenge of turkey poults with Mycoplasma iowae.

Day-old poults were inoculated orally each day for 7 days with 0.2 ml of Mycoplasma iowae, strain D112, 10(8) colony-forming units/ml. Cloacal swabs were taken from each poult during the inoculation period and at selected intervals until 21 days after the last inoculation. Most poults shed mycoplasmas persistently after inoculation. Cloacal swabs from eight out of ten poults were positive at 21 days after the last inoculation. Feces of poults in the infected group were normal, and there was no significant rise in cloacal temperature. At necropsy, mycoplasmas were recovered from tissues of the respiratory tract, gastrointestinal tract, spleen, and kidney. In the gastrointestinal tract, the most frequent recoveries were from the wall of the distal portion of the small intestine, cecum, and large intestine. Recovery of M. iowae from these organs and tissues indicated infection following oral challenge.

Animals↗