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Biomedical subjects

S Rosendal

Publications and source records attributed to S Rosendal.

At least 109 records · Page 6Linked to original sources
PubMed1981

Serotyping and detection of Haemophilus pleuropneumoniae by indirect fluorescent antibody technique.

This report provides a description and evaluation of the indirect fluorescent-antibody technique for serotyping and detecting Haemophilus pleuropneumoniae. The indirect fluorescent-antibody technique was serotype-specific when reference strains and sera were tested. Sixty-five field isolates were serotyped by indirect fluorescent-antibody technique and belonged to types 1,2 an 5. Two isolates did not fit any of the five established types. Twenty-three of the 65 isolates were also typed by immunodiffusion and the two tests agreed completely. Haemophilus pleuropneumoniae was detected in freshly-fixed impression smears from 12 lungs having acute pleuropneumonia. The identity of the bacteria was subsequently confirmed by cultural procedures. Fixed smears of cultured Haemophilus bacteria can be stored or mailed at -20 degrees to +24 degrees C without losing stainability.

Animals↗
PubMed1981

Vaccination against pleuropneumonia of pigs caused by Haemophilus pleuropneumoniae.

A strain of Haemophilus pleuropneumoniae was isolated from a pig with pleuropneumonia from a herd where this condition was frequent. A formalin inactivated culture of this isolate was used as antigen in two vaccine preparations: A and B. Vaccine A had peanut oil + arlacel 80 + tween 80 as adjuvant and vaccine B had aluminum hydroxide gel as adjuvant. Twenty pigs were vaccinated twice with vaccine A and 19 with vaccine B. Twenty additional pigs were not vaccinated. All pigs were transferred to the herd. Eleven pigs in the nonvaccinated group developed pneumonia and seven of these died within eight days after exposure. None of the vaccinated pigs had signs of pneumonia. It is concluded that the vaccines prevented the acute form of pleuropneumonia due to H. pleuropneumoniae.

Animals↗
PubMed1980

Factors associated with mortality in feedlot cattle: the Bruce County Beef Cattle Project.

The design of and the analysis of data from the Bruch County Beef Cattle Project, particularity the field study, are presented and discussed. A major value of the study may lie in its ability to relate laboratory findings to events in the field. The factor most strongly related to mortality was ration, with cattle on hay based rations having a much lower mortality on average than cattle on corn based rations. In general, management activities associated with growing cattle (as opposed to fattening) over the first winter were sparing for mortality. Groups of cattle given prophylactic vaccines or drugs within two days of arrival tended to experience higher mortality (not statistically higher) than those cattle not receiving those measures, or cattle groups recieving the same prophylactics more than 48 hours after arrival. However, these results require validation in the remaining years of the study. Fibrinous pneumonia was the most frequent diagnosis in the calves on postmortem examination.

Animal Feed↗
PubMed1978

Canine mycoplasmas: pathogenicity of mycoplasmas associated with distemper pneumonia.

Mycoplasmas of the following species were isolated from the lungs of dog with distemper: Mycoplasma cynos, Mycoplasma bovigenitalium, Mycoplasma canis, Mycoplasma spumans, and Mycoplasma gateae. One-week-old dogs were inoculated endobronchially with cloned cultures of each species. M. cynos induced a focal pneumonia that were characterized histologically by severe inflammation of bronchi and adjacent respiratory tract tissue. The inflammatory response was initially neutrophilic and later was histiocytic with interstitial lymphoid hyperplasia. M. cynos was recovered from the lungs of inoculated dogs until three weeks after inoculation. A mild inflammatory response was observed in lung tissue of the dog inoculated with M. bovigenitalium, whereas M. canis, M. spumans, and, M. gateae did not induce any visible lesions.

Animals↗
PubMed1978

Isolation of mycoplasmas from the canine genital tract: a survey of 108 healthy dogs.

Cultivation of preputial or vaginal swab samples from 108 apparently healthy dogs gave presumptive mycoplasma growth in 72 cases. Specific identification of 58 mycoplasma isolates based on biochemical studies, indirect immunofluorescence and growth inhibition tests showed association with eight different species or groups, only four of which had previously yielded genital isolates. Fourteen strains were related to M bovigenitalium.

Animals↗
PubMed1977

Experimental mycoplasmal pneumonia in dogs: electron microscopy of infected tissue.

Thin sections of lung tissue from dogs with pneumonia induced by endobronchial inoculation of Mycoplasma cynos were examined by electron microscopy. Mycoplasmas were observed extracellularly in lumen of bronchus and in alveoli in the earlier stages of the infection. The infection also resulted in degenerative changes in the bronchial epithelial cells, including in particular destruction and loss of cilia.

Animals↗
PubMed1975

Canine mycoplasmas: cultural and biochemical studies of type and reference strains.

Media and conditions for cultivation of type and reference strains of the mycoplasmas presently known (except M. bovigenitalium, A. laidlawii and Ureaplasma strains) to occur in dogs are described. The colonial morphology, ultrastructure, filterability and absence of reversion confirmed that the strains belong to the order of Mycoplasmatales. All strains were cholesterol dependent and therefore of the family Mycoplasmataceae, genus Mycoplasma. Finally the most useful biochemical characteristics are specified.

Animals↗
PubMed1975

Canine mycoplasmas: serological studies of type and reference strains, with a proposal for the new species, Mycoplasma opalescens.

The type and reference strains of all Mycoplasma species or "serogroups" presently known to occur in dogs were compared serologically. Tests of growth inhibition and metabolism inhibition as well as indirect immunofluorescence tests did not disclose any cross reactions. Examination by double immunodiffusion revealed at least one antigen specific for each strain tested. In addition, the common antigens were found within the glucose fermenting group and within the arginine metabolizing group of Mycoplasma strains, respectively. No antigens were shared by these groups. The arginine positive strain MH5408, serogroup D was found to differ serologically from all other arginine positive Mycoplasma species. Serogroup D is therefore a new species for which the name Mycoplasma opalescens is proposed. The type strain is MH5408 (ATCC 27921 and NTCC 10149). Finally, serological data are presented which relate strain HRC689 to M. mycoides.

Animals↗