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Biomedical subjects

S Pastore

Publications and source records attributed to S Pastore.

42 records · Page 3Linked to original sources
PubMedDate not supplied

Mechanisms of immune-mediated skin diseases: an overview.

The skin is a frequent site of pathological immune responses that can take place in the dermal and/or the epidermal compartments.These immunopathological reactions often occur towards innocuous antigens and may be the result of T cell-dependent and/or autoantibody dependent mechanisms. Defective immune regulation is increasingly recognized as very relevant in many skin and systemic immune-mediated disorders. In some instances (e.g., psoriasis and atopic dermatitis) genetic predisposition can affect also the capacity of keratinocytes to initiate or perpetuate inflammatory responses. A more precise understanding of the molecular and cellular mechanisms underlying each disorder may allow the identification of novel targets for more effective therapeutic strategies.

Autoantibodies↗
PubMedDate not supplied

Mitoxantrone in combination with etoposide and cytarabine for treatment of poor prognosis acute non lymphoid leukemia patients.

BACKGROUND: The proved effectiveness and relatively low extrahematological toxicity of Mitoxantrone, Ara-C and Etoposide as single agents and in combination in the treatment of acute non lymphoid leukemia (ANLL) are well established. In a phase II study the efficacy and toxicity of an induction combination regimen with Mitoxantrone, Ara-C and Etoposide were evaluated for treatment of poor risk ANLL patients. METHODS: Twenty-seven poor prognosis ANLL patients were treated with Mitoxantrone, 7 mg/sm days 1-3; VP16, 150 mg/sm days 1-3; Ara-C, 200 mg/sm continuous infusion days 1-5. Median age of treated patients was 63 (17-78): 10 de novo leukemias (4 greater than 65 yrs 3 with cardiomyopathy); 12 secondary leukemias (5 secondary to a myelodysplastic syndrome, 5 to myeloproliferative syndrome, 2 to other neoplasias); 3 relapses; 2 refractory. RESULTS: Fifteen patients (55.5%) achieved CR (8 de novo leukemias, 3 relapses, 4 secondary leukemias); 7 died in induction, 5 progressed. Median remission duration was 27 weeks (range 5-70 wks) and overall median survival 13 weeks (range 4-80). Extraematological toxicity was low, but five patients died from infections and two from hemorrhages. CONCLUSIONS: The combination tested in this trial proved effective in the treatment of "poor risk" ANLL, but the results need to be confirmed on greater numbers of patients, mainly in the group of de novo leukemias that can't be treated with more aggressive regimens.

Antineoplastic Combined Chemotherapy Protocols↗
PubMedDate not supplied

Rapid pentachlorophenol evaluation in solid matrixes by second derivative UV spectroscopy for application to wood and leather samples.

A method for the quail-quantitative evaluation of pentachlorophenol (PCP) in solid matrixes has been developed. The procedure is based on solid-liquid extraction of solid samples (leather or wood), followed by purification on a cyanopropyl column and determination of the preservative by second derivative UV spectroscopy considering the PCP A peak-through value (304-297 nm). The method allows rapid PCP determination in the concentration range 1-40 micrograms/mL; any matrix interference is avoided by the purification step and recoveries of the preservative were 99.12% (RSD% 0.13) for the leather matrix and 98.03 (RSD% 0.17) for the wood matrix.

Microchemistry↗
PubMedDate not supplied

Fibroblast-keratinocyte co-cultures in vitro: growth, morphometry and nutrient exchange.

The interaction between fibroblasts and rat keratinocytes co-cultured in vitro was examined. The epidermal cells cultured with a basal medium or with a conditioning medium (derived from fibroblast cultures) presented a lower growth rate and significantly greater cellular dimensions than those cells grown in the presence of a feeder layer. Qualitative and quantitative differences in the keratin patterns of the cells grown in the three cultural conditions were found. Keratinocytes cultured with the feeder layer expressed seven keratin types (58, 57, 53, 52, 50, 48 and 45 kDa), those with conditioning medium, five types (58, 53, 52, 48 and 45 kDa) and those with basal medium, only one type (45 kDa). This data confirms the dependence of keratinocytes on fibroblasts. HPLC analysis of the culture media, suggested that a protein factor (MW approximately 65 kDa) was secreted by fibroblasts into the culture medium. This factor was added to the keratinocyte culture medium (conditioning medium), and, after a 48 hour culture, was apparently completely removed from the medium by the keratinocytes. Moreover, the keratinocytes cultured with the feeder layer and exposed to indomethacin, a cyclo-oxygenase inhibitor, showed a decrease in growth rate at drug concentrations of < or = 10 microM which did not induce a reduction in the viability of the fibroblasts and keratinocytes in separate cultures. This preliminary data suggests a relationship between fibroblast PGE2 secretion and keratinocyte growth.

3T3 Cells↗
PubMedDate not supplied

Interaction between keratinocytes and fibroblasts cultured in vitro: morphology, morphometry and growth.

In order to elucidate the interactions between fibroblasts and keratinocytes co-cultured in vitro, the morphology and growth of rat keratinocytes cultured with and without a feeder layer were compared. Morphological analysis was coupled with a simple morphometric method of image analysis performed with a computer aided system to demonstrate statistically significant differences between keratinocyte cultures obtained with and without a feeder layer. Epidermal cells cultured without fibroblasts but with a conditioning medium showed a slower growth rate and significant a greater cellular dimensions in comparison with cells grown in the presence of a feeder layer. These results suggest that the conditioning medium induces a morphological change of keratinocytes. The electrophoretic patterns of proteins extracted from cells cultured with and without a feeder layer were identical, whereas densitometric analysis revealed that the quantitative expression of proteins was clearly different. These data confirm that of keratinocyte growth is dependent on fibroblasts or fibroblast products.

Animals↗