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Biomedical subjects

S Ono

Publications and source records attributed to S Ono.

At least 757 records · Page 42Linked to original sources
PubMed1984

Heterologous enzyme immunoassay for puromycin aminonucleoside using beta-D-galactosidase as a label.

A heterologous enzyme immunoassay (EIA) was developed to quantify puromycin aminonucleoside (PA). This double antibody assay was based on the use of anti-puromycin (PU) antibody and used beta-D-galactosidase-labeled PA conjugate prepared via N-(m-maleimidobenzoyloxy)succinimide. The standard curve of the assay ranged from 1 ng to 30 ng, and the lower limit of detection was 22.7 nM (1 ng/tube). The EIA was found to be approximately 20 times more sensitive than the homologous EIA for PA with anti-PA antibody and PA-beta-D-galactosidase conjugate. The heterologous EIA was free from interference by any purine or pyrimidine analogs and drug levels were easily determined in rat tissue following i.v. administration at a dose of 15 mg/kg. The sensitivity and specificity of the EIA should provide a valuable new tool for use in pharmacokinetic and toxicity studies of PA.

Animals↗
PubMed1984

Synergistic effect of subteratogenic doses of antisera on the development in the rat.

Wistar rats were administered rabbit anti-rat-umbilical-cord serum (AUCS) alone on day 9 of gestation or in combination with subteratogenic doses of rabbit anti-rat-kidney serum (AKS). The results showed that the incidence of malformation was low (13.1% +/- 7.0 S.E.) even when very high doses of AUCS alone were administered. However, the incidence markedly increased to 61.0% +/- 9.3 S.E. when a small dose (0.10 ml/100 gm) of AKS was administered on day 9 followed by 0.90 ml/100 gm of AUCS on day 10. The malformations included microphthalmia, hydrocephaly, aortic arch anomalies, ventricular septal defect, and anophthalmia, being similar to the effects of a medium dose of AKS. These results suggest that a combination of subteratogenic doses of teratogenic antisera produces malformations, possibly by mechanisms that potentiate the teratogenic action of the sera.

Abnormalities, Drug-Induced↗
PubMed1984

Circulating immune complexes and granulocytes chemotaxis in Kawasaki disease.

Serum immunoglobulin levels, complement titers, circulating immune complex levels, and chemotaxis of granulocytes were evaluated in 32 patients with Kawasaki disease with or without coronary aneurysm. The group of patients with coronary aneurysm showed relatively higher levels of IgG. Regardless of the presence of coronary aneurysm, the level of IgE in the acute phase was higher than that in the convalescent phase. The level of immune complexes was higher in the group of patients with coronary aneurysm (p less than 0.05). There was a low negative correlation between immune complexes and CH50. The chemotaxis of the patients with coronary aneurysm was significantly impaired (p less than 0.01).

Aneurysm↗
PubMed1984

OP-1206, a prostaglandin E1 derivative. Effects of oral administration to patients with chronic lung disease.

In order to assess the clinical effectiveness of an oral prostaglandin E1 derivative, OP-1206, five patients with chronic obstructive pulmonary disease and two with pulmonary fibrosis were studied from the standpoint of hemodynamics and nine others with chronic lung disease from the standpoint of respiratory function. Oral intake of OP-1206 resulted in a significant decrease in the pulmonary arterial pressure (p less than 0.01), total pulmonary vascular resistance (p less than 0.01), pulmonary arteriole resistance (p less than 0.01), and total systemic vascular resistance (p less than 0.05), and an increase in the cardiac index (p less than 0.05) and oxygen delivery (p less than 0.01) with insignificant changes of PaCO2, PaO2 and pH. There was no clinical improvement of lung function after OP-1206 intake. OP-1206 is a potent vasodilator, improving cardiac performance in patients with chronic lung disease and possibly preventing the progress of cor pulmonale in this kind of patient.

Aged↗
PubMed1984

FAD-induced in vitro activation of glutathione reductase in the lens of B2 deficient rats.

We studied the FAD-induced in vitro stimulation of lenticular glutathione reductase in riboflavin-deficient rats. The stimulatory effect of FAD on lenticular glutathione reductase in rats fed a B2-deficient diet for 4 weeks was remarkably higher than in paired control rats fed a B2-supplemented basal diet and control rats had ad libitum access to a B2-supplemented basal diet. The in vitro FAD stimulation effect on rat lenticular glutathione reductase represents a sensitive indicator of the B2 deficient status.

Animals↗
PubMed1984

[Fundamental study on ceftriaxone].

Ceftriaxone (CTRX) was examined in the blood level and urinary excretion between a healthy group of 2 persons receiving 1 g CTRX for 1 day and the other of 3 patients with renal failure receiving 1 g for 3 days, both by intravenous injection or intravenous drip infusion. In the healthy group, the blood half-life time of CTRX was 6.0 hours in 1 person and 8.2 hours in the other, being 7.1 hours on average. The mean blood level in the healthy group was 199 micrograms/ml at peak and was 13 micrograms/ml at 24 hours after administration. In patients with renal failure, the peak blood level ranged from 136.8 to 161.1 micrograms/ml on the 1st day, from 163.1 to 217.0 micrograms/ml on the 2nd day and from 156.4 to 189.7 micrograms/ml on the 3rd day, showing no tendency of getting higher, while the bottom level did from 15.2 to 47.4 micrograms/ml on the 1st day, from 23.3 to 67.9 micrograms/ml on the 2nd day and from 10.9 to 72.6 micrograms/ml on the 3rd day. The urinary excretion rate was 54.6 +/- 3.7% on average in the healthy group while it ranged from 13.7 +/- 1.8 to 27.9 +/- 7.9% in the patient group. CTRX was effective especially against E. coli among the strains clinically isolated from the patients with renal failure. No side effects were observed in any case.

Adult↗
PubMed1984

[Bilateral primary germ cell tumors of the testis: report of two cases and review of the literature].

Bilateral germ cell tumors of the testis are not common. The contralateral tumor may develop simultaneously or sequentially. In both cases, bilateral involvement is postulated to represent two primary tumors. Case 1: A 47-year-old male was seen with a painless nodular mass in the right testis. Physical examination revealed hard swelling of both testes besides a nodule in the right testis. He underwent bilateral radical orchiectomy; and, microscopical both tumors proved to be seminoma. Although bipedal lymphangiography was negative for nodal metastases, postoperative irradiation to the pelvic and para-aortic lymph nodes was given to a total dose of 5,800 rad. The patient was alive 15 years after treatment for bilateral testicular tumors. Case 2: A 53-year-old male presented with painless left scrotal swelling. Physical examination showed not only an enlarged hard testis in the left scrotum but also a palpable hard mass in the right testis. Bilateral radical orchiectomy was performed and pathological examination revealed pure seminoma in both testes; a 1 cm mass in the right testis and almost completely replaced tumor in the left testis. Evaluation including retroperitoneal lymph node dissection revealed no metastatic disease. Postoperatively, the patient received 1,900 rad of irradiation to the retroperitoneal space. He is well one year after surgery and has no evidence of recurrence or metastasis. Previous reports of bilateral germ cell testicular tumors are reviewed and the incidence, age, predisposing factors, interval between the first and second tumors, histology, pathogenesis, prognosis, and management are discussed.

Age Factors↗
PubMed1984

Effect of steroidal sex hormones on the sex-related differences in the hepatic activities of gamma-glutamyltranspeptidase, glutathione S-transferase and glutathione peroxidase in rats.

The activities of gamma-glutamyltranspeptidase (gamma-GTP) and glutathione peroxidase (GSH-Px) were higher in female than in male rat liver. Glutathione S-transferase (GST) activities were conversely higher in males than females. Estradiol administration to castrated rats enhanced the gamma-GTP activity. Castration produced a significant increase in GSH-Px activities which was reduced to the control level by subsequent testosterone treatment. Ovariectomized rats had markedly increased the GST activity. These results suggest that GSH-related enzymes studied here may be subject to modulation by androgenic and estrogenic influences.

Animals↗
PubMed1984

Effects of riboflavin administration on the phospholipid metabolism of rat liver impaired with carbon tetrachloride.

This study was undertaken to investigate the effects of riboflavin administration on the phospholipid metabolism of rat liver impaired with carbon tetrachloride. Although the specific radioactivity in the CCl4 group decreased, the groups pretreated with riboflavin, especially B2-butyrate group, were prevented a decrease of 32P incorporation into total phospholipid in liver. The percentage of 32P incorporation into phosphatidylcholine in both mitochondria and microsomes was increased by the administration of riboflavin.

Animals↗