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S Okamura

Publications and source records attributed to S Okamura.

At least 289 records · Page 16Linked to original sources
PubMed1982

[Clinical diagnosis of well differentiated cervical adenocarcinoma with plentiful mucous secretion].

Six cases of cervical adenocarcinoma with plentiful mucous secretion were recently experienced. They were clearly distinguishable from the majority of adenocarcinoma without plentiful mucous secretion from the standpoint of clinical and colposcopic findings. As common features deduced from clinical and colposcopic findings, it could be pointed out that portio vaginalis become swollen as a whole showed resiliency as of a rubber ball, and that erosion widespread over nearly entire surface of portio vaginalis is accompanied with a plentiful mucus. On the surface of erosion was found neither localization of ulcer, nor necrosis and nor bleeding trend even in cases under progressing. Colposcopic findings were distinguished with features of papillea-, net-and transformation zone-like, and mixed type. On the other hand, all the cases were evaluated cytologically to be positive with difficulty. And they were histologically well differentiated adenocarcinoma with plentiful mucus. The distinctive feature in the clinical and colposcopic findings identified for the cases of well differentiated adenocarcinoma with a plentiful mucus was evaluated to be useful means to assist the diagnosis of cervical adenocarcinoma.

Adenocarcinoma, Mucinous↗
PubMed1982

[A preliminary report of computerized ultrasonography in obstetrics and gynecology: a new technique of C-mode (author's transl)].

UNLABELLED: In order to obtain a three-dimensional ultrasonogram as a first step for a general visualizing diagnosis, a new computerized ultrasonography was tried with patients from our obstetric and gynecological department, with the following results: 1. Time required: Collection of data for the three-dimensional part measuring 17 X 17 X 8.5cm took 17 seconds (Scanning time), and formation of the 32-image longitudinal sections and coronal sections (C-mode) took 46 seconds and 1 minute 54 seconds, respectively. Formation of the 64-image longitudinal sections and coronal sections (C-mode) took 1 minute 20 seconds and 3 minutes 20 seconds, respectively. 2. Images: The recorded and reproduced images were identical to the originals macroscopically and even as indicated on the line printer. The coronal images (C-mode) and the longitudinal images could well stand practical use. 3. Reproduction of images: All data were stored on magnetic tape which could be processed as needed. 4. CLINICAL RESULTS: (a) In obstetric patients, the images enabled us to confirm the location and expansion of the placenta and fetus more clearly. (b) In gynecological patients, it proved to be easy to get a whole view permitting better comprehension of the tumor shape, its internal components and its relation with the surrounding organs in a three-dimensional way.

Adult↗
PubMed1981

Heterogeneity of human thymocytes and a malignant T-lymphoblast cell line, MOLT-3.

The purpose of this paper was to study the heterogeneity of human thymocytes and leukemic cells of the T-cell line MOLT-3 by velocity sedimentation. Analysis of the subpopulations of thymocytes demonstrated that they represent a heterogeneous population of cells with respect to their size, proliferative activity, and presence and quantities of terminal deoxynucleotidyl transferase and human thymus leukemia-associated antigen, a thymic isozyme of adenosine deaminase (HThy-L/ADA). Only a minor subpopulation of thymocytes (large cells) was in active cycle. The highest level of HThy-L/ADA was associated with the main subpopulation of thymocytes sedimenting at 3 to 4 mm/hr while low amounts of the HThy-L/ADA antigen (enzyme) were found in the minor fractions of the small and large cells. The distribution of terminal deoxynucleotidyl transferase-positive cells indicated that most, but not all, thymocytes contain the enzyme. Analysis of the T-cell line MOLT-3 showed that these cells could be separated into subpopulations with different biochemical and biological properties. More than one subpopulation of cells was capable of DNA synthesis. In contrast to the thymocytes, all fractions of MOLT-3 cells contained high amounts of HThy-L/ADA. The proportion of terminal deoxynucleotidyl transferase-positive cells as a function of sedimentation velocity was also quite constant although there was a slight but reproducible drop in the percentage of these cells in the slowly sedimenting fractions. The percentage of cells with receptors for sheep erythrocytes also remained high in fractions separated on the basis of size, although a consistently higher percentage was found in smaller cells. These studies indicated that thymus cells as well as the malignant T-cell line MOLT-3 can be separated on the basis of sedimentation velocity into subpopulations with different biological and biochemical properties. The data also indicated that the heterogeneity of MOLT-3 line cannot be explained solely on the basis of volume changes due to cell cycle, suggesting that they may represent heterogeneous populations of cells.

Adenosine Deaminase↗
PubMed1980

Single-cell immunofluorescence assay for terminal transferase: human leukaemic and non-leukaemic cells.

The characteristics of a single-cell immunofluorescence assay for terminal deoxynucleotidyl transferase (terminal transferase, TdT) is described. The data indicate that the single-cell immunofluorescence assay is highly efficient and specific for the detection of cells containing TdT. Using this assay, we have examined 124 marrow or peripheral-blood samples from 104 patients with or without haematological malignancies. Results indicate that TdT(+) cells from 6% to 100% were found in the following patients: 34/40 samples from patients with ALL at the time of diagnosis or during relapse; 2/3 patients with acute undifferentiated leukaemia; 2/3 patients with acute myelomonocytic leukaemia; 1/24 patients with acute myeloblastic leukaemia; 1/5 patients with chronic myelocytic leukaemia (CML) in blastic crisis; and 2/2 patients with diffuse lymphoblastic lymphoma. In contrast less than 1% of TdT(+) cells were found in 20 marrow or peripheral-blood samples from ALL patients in complete remission; 8 patients with CML in chronic phase; 2 patients with myeloma; 1 sample from a patient with Hodgkin's disease, peripheral-blood samples from 7 normal donors and marrow samples from 6 patients without haematological malignancies. TdT(+) cells were also found in association with cells with lymphoblast morphology. The TdT(+) cells in marrow were shown to be directly correlated with the percentage of morphological lymphoblasts, with a Spearman rank coefficient of 0·81, significant at a 0·001 level. In 2 longitudinal studies of 2 ALL patients with TdT(+) cells at diagnosis, the percentage TdT(+) cells also changed in parallel with the proportion of lymphoblasts. However, studies of 2 other patients with morphologically diagnosed ALL with < 1% TdT(+) cells at diagnosis also showed < 1% TdT(+) cells throughout the period studied, indicating a stable phenotype of blast cells in these patients. The single-cell immunofluorescence assay for TdT, which requires < 0·1% of the cells used in a conventional biochemical assay, is highly specific, and could provide a technically more efficient alternative for use in clinics as well as in experimental investigations of subpopulations of leukaemic and normal marrow cells.

Bone Marrow↗