Search PubMed⌕ Search

Biomedical subjects

S Noguchi

Publications and source records attributed to S Noguchi.

At least 631 records · Page 35Linked to original sources
PubMed1983

[Clinical effect of cefroxadine on surgical infections].

Cefroxadine (CXD), an orally active cephalosporin antibiotic, has a broad spectrum and a bactericidal action. The efficacy of CXD in the surgical field was investigated and the following results were obtained. CXD was administered to 31 cases in all; 13 cases with mastitis, 9 with wound infection, 4 with infected atheroma, 3 with periproctal abscess and 2 with phlegmon, respectively. The daily dose was ranged from 750 mg to 1,500 mg. Clinical effects were good in 27 cases and fair in 4 cases, and the effective rate was 87.1%. As to side effects, a slight diarrhea was observed in 1 case, but the symptom was disappeared after 2 days without a special treatment.

Administration, Oral↗
PubMed1982

Characterization of a rat tRNA gene cluster containing the genes for tRNAAsp, tRNAGly and tRNAGlu, and pseudogenes.

The putative genes for tRNAGAUAsp(C), tRNAGGAGly(G) and tRNAGAGGlu are in a cluster on the rat chromosome and are present exclusively in a 3.3 kb region cleaved with a restriction endonuclease EcoRI. The cluster reiterates about 10 times on the haploid DNA. Four lambda clones each containing an independent repeating unit were isolated from a rat gene library. The studies on the cloned DNA revealed that the length of the repeating unit including the 3.3 kb EcoRI fragment was at least 13.5 kb. Nucleotide sequence analysis of the 3.3 kb DNA in the isolated clones showed sequence variations among the repeating units and incomplete genes for tRNAGly and tRNAGlu within the clusters.

Animals↗
PubMed1982

Isolation and characterization of an Escherichia coli mutant lacking tRNA-guanine transglycosylase. Function and biosynthesis of queuosine in tRNA.

An E. coli mutant that lacks tRNA-guanine transglycosylase was isolated by random screening from a collection of Escherichia coli mutants obtained with N-methyl-N'-nitro-N-nitrosoguanidine. The defective gene, named tgt, was mapped at about 9 min on the E. coli chromosome, and the gene order was shown to be phoB-tgt-tsx. tgt was transferred to an E. coli strain with a defined genetic background by P1 transduction to investigate its function. The mutant thus obtained lacked queuosine (2-amino-5-[3S, 4R, 5S)-4,5-dihydroxycyclopent-1-en-3-ylaminomethyl]-7-(beta-D-ribofuranosyl)-pyrro lo-[2,3-D]-pyrimidin-4-one) in tRNA, indicating that the enzyme is actually involved in the biosynthesis of queuosine in tRNA. No clear biological defect was observed in the mutant, and, in fact, it grew slightly faster than the control isogenic strain. tRNATyr lacking queuosine, isolated from the mutant, showed no significant biological difference from normal queuosine-containing tRNA in amino acid acceptor activity or amino acid transfer in a cell-free protein synthesizing system directed by synthetic polynucleotide. The only phenotypic change observed in the mutant thus far is marked reduction of viability when the cells are kept under unsuitable conditions for growth, suggesting that the presence of queuosine in tRNA is important to E. coli for survival in the natural environment.

Escherichia coli↗
PubMed1982

Restriction map of double-stranded DNA copy synthesized from poliovirus Sabin 1 RNA.

Nearly full-sized double-stranded cDNA was prepared from virion RNA of poliovirus Sabin 1 (LSc, 2ab) strain using reverse transcriptase. The double-stranded cDNa was cleaved at four and two sites by the restriction endonucleases Bam HI and Hind III, respectively. Based on the cleavage patterns of double-stranded cDNA into segments of various lengths, each of which had the sequence corresponding to that of the 3'-end of the viral genome, the location of each restriction fragment was determined. The cDNA fragments were cloned with pBR322 as a vector and some of their nucleotide sequences were determined. The DNA sequence indicated that the restriction map obtained was consistent with the known arrangement of viral RNA fragments (1-3). Comparison of the nucleotide sequences of the cloned Hind III (460 bases) and Pst I (434 bases) fragments with those of the corresponding region of Mahoney type 1 genome (3) revealed 12 point-mutation sites.

Base Sequence↗
PubMed1982

Characterization of a rat tRNA gene cluster containing the genes for tRNAAsp, tRNAGly, and tRNAGlu, and pseudogenes.

Molecular cloning and sequence analysis of a rat DNA fragment hybridized to purified tRNAAsp revealed that the genes for tRNAAspGAU(C), tRNAGlyGGA(G), and tRNAGluGAG are in a cluster on the rat chromosome. These genes are arranged in this order and are regularly separated by DNA regions of about 450 bp. The cluster is reiterated about 10 times on the haploid DNA and is present exclusively in a 3.3 kb region cleaved by restriction endonuclease EcoRI. Five lambda clones each containing an independent repeating unit were isolated from a rat gene library. The clones revealed that the length of the repeating unit including the 3.3 kb EcoRI fragment was at least 13.5 kb. Nucleotide sequence analysis and in vitro transcription of the 3.3 kb DNA in the isolated lambda clones showed sequence variations among the repeating units and the presence of incomplete genes for tRNAGly and tRNAGlu within the clusters.

Animals↗
PubMed1981

Surgical treatment for Graves' disease: a long term follow-up of 325 patients.

Postoperative thyroid status was classified into 6 categories on the basis of serum free thyroxine index (FTI), serum triiodothyronine (T-3) and serum TSH concentration. Review of 325 patients who underwent thyroidectomy for Graves' disease more than 4 years previously showed that 25 patients (7.7 per cent) had recurrent hyperthyroidism. Six patients (1.8 per cent) were classified as equivocal hyperthyroid since either FTI or T-3 was above the normal range. Two hundred and twenty-two patients (68.3 per cent) were unequivocally euthyroid and 33 patients (10.3 per cent) were euthyroid with elevated TSH levels. Twenty patients (6.2 per cent) were equivocal hypothyroid since either their FTI or T-3 values were below the normal range and TSH were increased. Hypothyroidism was present in 19 patients (5.8 per cent), of whom 11 had no clinical manifestation of thyroid dysfunction. The incidence of hypothyroidism did not correlate with the intervals between operation and review. The second review, performed in 189 patients 18 months after the first, showed that there was a change in thyroid functional status in 46 patients, of whom 32 had an increased level of function, including one hypothyroid and 7 equivocal hypothyroid patients who became euthyroid spontaneously. Thus postoperative hypothyroidism in some patients can recover without medication. Also thyroid function in some postoperative patients is not maintained at a fixed level but may fluctuate.

Adult↗
PubMed1981

[Studies on the diagnosis of uterine tumor by 201-thallium-chloride (author's transl)].

Cases of gynecological tumor mainly uterine benign and malignant tumors were evaluated using 201Thallium-Chloride (201Tl-Cl) after intravenous injection. The positive scans of 100% in 42 cases of uterine myoma including 29 cases of leiomyoma, 8 cases of adenomyosis and 5 cases of combined type myoma, also 100% in 8 cases of body cancer indicating adenocarcinoma and 25% in 12 cases of cervical cancer indicating squamous cell carcinoma were observed. The smallest focal lesion for the positive scans was needed approximately more 5 cm in diameter in the cases of leiomyoma. The most accumulation of activities was showed in adenomyosis or adenocarcinoma with much adenoid structure in the pathological study. Accumulation of 201Tl in uterine benign and malignant tumors was suggested the analog of potassium. The mechanism of tumor affinity was correlated to perfusion volume and the acceleration of the potassium metabolism in a tumor. On the other hand, we suggested the diagnosis of the malignant uterine tumor should be attended due to 201Tl was accumulation also in the benign uterine tumor. The uterine scanning using 201Tl-Cl was acknowledged to be very useful and safe indicating early detection at 20 minutes after the injection, (non-traumatic procedure and non-visualized bone tissues). In conclusion, this radioisotopical procedure was indicated high value of screening examination in the diagnosis of uterine tumor.

Adult↗
PubMed1980

Factors affecting the prognosis of patients with carcinoma of the thyroid.

Of 763 patients with carcinoma of the thyroid who had undergone a primary operation between 1948 and 1970, 705 were observed for more than five years. Included were 545 patients with papillary carcinoma. 123 with follicular carcinoma, 59 with microcancer, 30 with undifferentiated carcinoma and six with medullary carcinoma. The relative survival rate for patients with pure undifferentiated carcinoma one year after operation was 27 per cent and that for those with mixed undifferentiated carcinoma was 24 per cent. For patients with differentiated carcinomas, the most important factors affecting the prognosis were age and sex. The 15 year survival rates for women less than 40 years of age with papillary or follicular carcinoma were 102 and 100 per cent, respectively, while for women more than 40 years with papillary or follicular carcinoma, they were 90 and 84 per cent, respectively. For men more than 40 years of age with papillary carcinoma, the relative survival rate was 67 per cent. The size of the tumor and the types of operation did not affect the prognosis in patients less than 40 years. The presence of chronic thyroiditis also had no effect upon the prognosis. The relative survival rate with tumors up to 99 grams in weight, after 20 years, was almost 100 per cent in women less than 40 years of age, while for women more than 40 years of age the survival rates were 95 per cent with tumors weighing less than 20 grams and 72 per cent with those weighing 20 to 99 grams, an indication that tumor size may affect prognosis in older patients.

Adenocarcinoma↗