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Biomedical subjects

S N Sharma

Publications and source records attributed to S N Sharma.

At least 37 records · Page 2Linked to original sources
PubMed1998

Blood meal identification by using Microdot ELISA in vector mosquitoes.

The blood meals of five mosquito species which are vectors for malaria, J.E. and Filaria were determined by using Microdot ELISA technique, which is a rapid and sensitive method. A total of 4354 freshly fed mosquitoes comprising of Anopheles culicifacies, An stephensi, Culex quinquefasciatus, Cx. vishnui gp. and Cx. tritaeniorhynchus were tested. Results of the study reveal that highest proportion of An. culicifacies between 76-100% fed on bovine host followed by 0.3 to 24.2% on human. Cx. vishnui showed 1.9% feeding on human, 49-87.7% on bovine and 7.4-12.3% on the pigs. It has been observed that malaria vectors namely An. culicifacies and An. stephensi are still zoophilic in nature. Similarly, the vector of J.E. namely Culex vishnui gp. and Cx. tritaeniorhynchus also fed on cattle but some samples have indicated feeding of blood meal from pigs.

Animals↗
PubMed1998

'Anti-malaria month'--a step forward in multisectoral collaboration and community involvement in malaria control.

For a long time malaria control in India has largely been a government responsibility with little involvement of the community at large, and other sectors of the economy in the control efforts. There is now increasing realisation that involvement of the community and a multi-sectoral approach should be essential components of the malaria control strategy. The National Malaria Eradication Programme is accordingly laying greater emphasis on coordination with other sectors of the economy, and changing community perceptions and health-related behaviour. A massive programme of community mobilisation with this objective in view has been taken up since 1997. A major component of this community mobilisation programme is the observance of an 'Anti-Malaria Month', during which, country wide awareness campaigns are undertaken at all levels of implementation, to promote inter-sectoral collaboration and community action for malaria control. The present paper summarises the rationale and messages of the 'Anti-Malaria month'.

Animals↗
PubMed1997

Studies of the hepatic mitochondrial and microsomal mixed-function oxidase system during Plasmodium yoelii infection and inducer treatment in Swiss albino mice.

Plasmodium yoelii infection resulted in depression of hepatic mitochondrial and microsomal mixed-function oxidase system indices, e.g. cytochrome P-450, cytochrome b5 and phase II detoxification enzyme glutathione-S-transferase, while heam and haemozoin registered a marked increase in Swiss albino mice. Phenobarbitone (inducer) treatment showed induced levels of hepatic mitochondrial and microsomal cytochrome P-450 and glutathione-S-transferase in normal as well as in infected mice. The induced cytochrome P-450 and glutathione-S-transferase activities were similar in normal and infected mice. The findings were further supported by the isoenzymic profile and drug-binding properties of the terminal monoxygenase, cytochrome P-450.

Animals↗
PubMed1994

Larvivorous capacity of some indigenous fish of Haryana state.

Studies were undertaken to assess the role of some indigenous fish of Haryana state for the biological control of mosquitoes. Total of 28 fish species were encountered. Only six fresh water fish species Puntius ticto, Colisa fasciata, Aplocheilus panchax, Rasbora daniconius, Chanda nama, and Esomus danricus species possessed good feeding potential on mosquito larvae. The small size, well developed eyes and position of the mouth seem to be probably the important factors helping their voracity on mosquito larvae. These fish are either surface, subsurface or column feeders.

Animals↗
PubMed1993

An improved dot ELISA to detect fowl adenovirus type-1 antigen.

An improved dot immunobinding assay to detect fowl adenovirus type-1 is described. The method consists of spotting of antigen on nitrocellulose membrane sheet, blocking with either 5% acetic acid or with 5% defatted milk powder and fixation of either antigen or antigen-antibody complex, with either 50% methanol or 0.25% glutaraldehyde or 0.2% tannic acid. The results revealed that fixation of both antigen and antigen-antibody complex resulted in 4-fold increase in sensitivity when acetic acid was used as blocking agent. The use of two substrates simultaneously resulted in more colour intensity and clarity than using both substrates separately.

Adenoviridae Infections↗
PubMed1993

Malaria in stone quarry area of Faridabad complex (Haryana).

An analysis of five-year (1987-91) epidemiological data showed that the stone quarries contributed, on an average, 38.7% of malaria cases to the total of Faridabad Town and 11.6% to that of Faridabad district. Various malariometric indices like Annual Parasite Incidence (API), Annual Blood Examination Rate (ABER), Slide Positivity Rate (SPR) and Slide falciparum Rate (SfR) were analysed to assess the endemicity of disease, and attempts were made to compare them with the malaria parameters of the town and then of the district. Migrant population working as labour in stone quarries resides in areas conducive to malaria and hence the need for intensive control measures.

Humans↗
PubMed1993

Role of An. culicifacies and An. stephensi in malaria transmission in urban Delhi.

Studies on adult densities, vector incrimination and breeding habitats of An. culicifacies and An. stephensi were carried out during June 1984 to May 1986 in three different ecological areas of urban Delhi. Both An. culicifacies and An. stephensi prevailed throughout the year in peri-urban areas with higher densities during the post-monsoon months. In south and northwest areas, low densities of these vector species were recorded. An. culicifacies was found naturally infected with sporozoites in peri-urban and south areas, whereas An. stephensi showed gland infection in south areas only. Out of 827 blood smears collected, 302 were positive for malaria parasites. Of the positives, P. vivax formed 48.3% and P. falciparum 51.7%. Peri-urban areas showed a higher number of malaria cases than south and northwest areas.

Animals↗