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Biomedical subjects

S N Sharma

Publications and source records attributed to S N Sharma.

At least 19 recordsLinked to original sources
PubMed1992

Effect of operation theatre environment on laparotomy wound infection in bovines.

Right flank laparotomies were performed on five clinically healthy cross-bred calves. A significant (P less than 0.05) increase of 22.1 and 2.7 times in bacterial count in subcutaneous tissue and incised skin edges, respectively, and a non-significant increase (1.1 times) in muscles and peritoneum had occurred during 15 minutes exposure to the operation theatre environment. Poly-morphonuclear cells and Gram-positive and Gram-negative bacterial infiltration was also demonstrated histopathologically in muscles and peritoneum. Organisms isolated from operation theatre air were Staphylococcus aureus, other Staphylococcus spp., Bacillus spp., Micrococcus spp., and yeast. Usually similar organisms were also found on the laparotomy wound, just before closure of abdomen. Operation theatre environment is concluded to be a significant source of operative wound contamination. Provision of separate operation theatres for different types of surgery in different species is recommended and the necessity for their regular disinfection is stressed.

Air Microbiology↗
PubMed1992

Development of medicated aerosol dressings of chlorhexidine acetate with hemostatics.

Aerosol formulations to act as medicated aerosol dressings have been prepared with an aim of forming a protective film over the wound after spraying combining the properties of antiseptics and hemostatics. Chlorhexidine acetate along with three hemostatics namely zinc acetate, methyl cellulose and Calendula tincture were used for the formulations. The formulations were found to be satisfactory in their performance and purpose.

Aerosols↗
PubMed1991

Cell mediated immune response of chicks following fowl adenovirus type-1 infection.

Cellular immune response of 6-week-old chickens infected with fowl adenovirus type-1 was evaluated by both in vivo and in vitro assay. In vitro assay, enumerating peripheral T lymphocyte by alphanaphthyl acetate esterase staining, revealed that cellular immunity appeared as early as 1 week post-infection and was maintained up to 5 weeks post-infection. In vivo assay by phytohaemagglutin skin test showed cellular immunity until 2 weeks post-infection. Thereafter no immunity was observed by this assay.

Adenoviridae Infections↗
PubMed1991

Comparative studies on the antigenicity of extra- and intracellular viruses of fowl pox.

The intra- and extracellular virus of three strains of fowl pox virus, when precipitated in succession with different saturation of ammonium sulphate revealed three antigens in gel diffusion test in the precipitates obtained at 25%, 50% and 75% of saturation. Further analysis of each positive antigen by dot ELISA revealed that the extracellular virus of FS-8 and HP 1 strains possessed excess antigenic protein at 50% saturation compared to their intracellular viruses. While no difference between extra- and intracellular viruses of FS-4 strain was observed.

Animals↗
PubMed1991

Observations on the breeding of anophelines in rice fields of Shahjahanpur district, Uttar Pradesh.

Studies on ecological succession and association of anophelines in selected paddy fields of Dadraul PHC were carried out in 1988 during the paddy cultivation period from June to October. Breeding of five anophelines was observed in paddy fields. Out of the adults that emerged from larval collections the percentage of An. subpictus was the highest followed by An. nigerrimus, An. culicifacies, An. annularis and An. barbirostris. Further, An. culicifacies and An. subpictus breeding occurred in the early stage of rice cultivation and stopped before the breeding of An. nigerrimus started i.e., nearly 30 days after rice transplantation. An. nigerrimus breeding continued till harvesting. An inverse correlation between larval density of both An. culicifacies and An. subpictus and the height of the rice plants was observed.

Animals↗