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Biomedical subjects

S Morikawa

Publications and source records attributed to S Morikawa.

At least 271 records · Page 15Linked to original sources
PubMed1982

Carcinoembryonic antigen producing cultured cell lines enable detection of autoantibodies in sera from patients with gastrointestinal cancer.

Of 16 human malignant tumor cell lines established in our laboratory, seven lines, including three gastric cancer cell lines derived from patients with cancer, were found to carry carcinoembryonic antigen on their cell surface, as determined by radioimmunoassay and indirect immunofluorescence. The existence of antibodies (IgG class) against the gastric cancer cell lines (HPE-GAC-T, -2, -3) and lung cancer cell line (HPL-Ad-K) in the sera of patients with gastrointestinal cancer (incidence 70.8%) was demonstrated by indirect immunofluorescence. In nonmalignant cases and healthy controls, the incidence was 7.7 and 3.2%, respectively. Specificity of the antibodies detected in the sera of patients with gastric cancer was examined by absorption and blocking test methods of immunofluorescence. Even though there was an apparent heterogeneity of the specificity among the antibodies, the detection of such antibodies may be a feasible and practical approach to a clinical diagnosis of malignancy.

Adult↗
PubMed1982

Phorbol ester-induced differentiation of human T-lymphoblastic cell line HPB-ALL.

12-O-Tetradecanoylphorbol-13-acetate (TPA), a potent tumor promoter, induced phenotypic differentiation in the human thymic acute lymphocytic leukemia cell line, HPB-ALL. Within 30 min of seeding in the presence of TPA, the cells formed a smooth round shape. After a 7-day exposure to TPA, most of the cells became smaller and reminiscent of large or atypical lymphocytes. Electron microscopic analysis evidenced morphological differentiation in TPA-treated HPB-ALL cells. Thymic antigens stained with monoclonal antibody OKT6 were dramatically reduced while Leu2a-positive cells were increased in the TPA-treated HPB-ALL cells. However, OKT3-positive cells did not appear in these TPA-treated cells for up to 7 days. Upon TPA-induced phenotypic differentiation, the growth rate of cells was significantly inhibited, their ability to incorporate DNA and RNA via 3H-labeled precursors was reduced, their ability to bind sheep red blood cell rosettes was significantly increased, and the proportion of terminal deoxynucleotidyl transferase-positive cells was decreased.

Antigens, Surface↗
PubMed1982

Spontaneous occurrence of atypical hyperplasia and adenocarcinoma of the uterus in androgen-sterilized SD rats.

In SD female rats sterilized by a single injection of testosterone propionate at 2 days after birth, the spontaneous occurrence of atypical hyperplasia and adenocarcinoma of the uterus was observed for a fairly long period (greater than 2 yr). Two atypical hyperplasias and 2 adenocarcinomas were detected in 25 androgen-sterilized rats (ASR) after 500 days of age; in contrast, in 111 normal control rats no abnormal uterine proliferation was detected during a 750-day observation period. These results indicate that a persistence of both hormone imbalances and dysfunctional uteri in ASR induces abnormal uterine proliferation at a late age.

Adenocarcinoma↗
PubMed1982

Antitumor activity of D-mannosamine in vitro: different sensitivities among human leukemia cell lines possessing T-cell properties.

D-Mannosamine is toxic to human malignant T-lymphoid cell lines derived from patients with T-cell leukemia. We observed heterogeneity of mannosamine susceptibility among those cell lines. The leukemic T-cell lines, subgrouped according to the degree of mannosamine inhibition on nucleic acid biosyntheses, were: Subgroup 1, HPB-MLT cells; Subgroup 2, CCRF-HSB-2 and HPB-ALL cells; and Subgroup 3, MOLT-4 cells. The most sensitive line, HPB-MLT, originated from the patient with adult T-cell leukemia. The cytotoxicity of mannosamine was potentiated by a fatty acid, sodium oleate, at concentrations that were noncytolytic, and the interaction between the two drugs was synergistic. These results would suggest that mannosamine induces changes in the membrane structure of the leukemia cells. Thus, the primary target of the tumoricidal activity of mannosamine may also be the cellular membranes.

Adult↗
PubMed1982

[Fundamental studies on high risk factor of endometrial carcinoma functional and histological abnormalities of ovaries and uteri in experimental anovulatory rats (author's transl)].

Anovulatory sterility is considered as one of high-risk factors of endometrial adenocarcinoma. We produced an animal model of anovulation in Sprague-Dawley rats and compared the changes of reproductive organs, such as ovary and uterus, of androgen sterilized rats (ASR) with normal rats (NR). The results obtained were as follow: 1) Vaginal opening was observed at 45 days of age in all of NR, however it was observed in 63% of ASR at 300 days of age. 2) Infertility rate of ASR was 98%, and all of ASR revealed persistent estrus. 3) The ovarian and uterine weights of ASR were smaller than those of NR. 4) Numerous vesicular follicles and absence of corpora lutes were characteristics in the ovary of ASR. 5) Severe hyperplasia of the endometrium was recognized in 5 of 49 ASR, including 2 atypical hyperplasia. 6) Serum progesterone levels of ASR was significantly lower than that of NR. Therefore E/P ratio increased in ASR. 7) The uterine sensitivity of ASR to gonadectomy and estradiol administration was reduced. These results indicate that uterus of ASR is tonically stimulated by estrogen and that androgenization also causes proliferative alterations in uterine morphology.

Animals↗
PubMed1982

[Experimental studies on spontaneous occurrence of uterine adenocarcinoma (author's transl)].

Abnormal uterine proliferation was not detected in all 111 control normal rats (NR) during 750 days' observation period. In androgen sterile rats (ASR), 4 squamous metaplasias were detected in 25 ASR at 70-100 days of age. Thereafter, 9 squamous metaplasias and 2 non-atypical hyperplasias were detected in 20 ASR at 200-360 days of age. At 500-750 days of age, 12 squamous metaplasias, one non-atypical hyperplasia, 2 atypical hyperplasias and 2 adenocarcinomas were detected. Histological examination revealed that one of adenocarcinoma was composed of well differentiated type, poorly differentiated type and squamous metaplasia. Another case was composed of poorly differentiated adenocarcinoma, squamous cell carcinoma and squamous metaplasia. Hormonal environment of ASR with adenocarcinoma was characterized by an increase in estrogen/progesterone ratio, as compared to NR. After 500 days of age, 2 pituitary, 6 mammary and 3 adrenal tumors were detected in 33 NR, whereas 2 pituitary and 2 mammary tumors were detected in 25 ASR.

Adenocarcinoma↗
PubMed1981

Glucocorticoid receptors and terminal deoxynucleotidyl transferase activities in leukemic cells.

Glucocorticoid (GC) receptor and terminal deoxynucleotidyl transferase (TdT) activities were studied in leukemia cells to investigate their diagnostic and therapeutic implications. Among cell lines with T-cell character, higher GC-receptor and TdT activities were found in T-ALL (HPB-ALL and ALL-Ichikawa) than in cells from adult pleomorphic T-cell leukemia (HPB-MLT). HPB-Null with pre-B cell-character exhibited moderate GC receptor but low TdT activity; Raji cells and CCRF-SB, derived from B-cell Burkitt lymphoma and B-ALL, respectively, manifested low GC receptor and no TdT activity. The highest GC receptor activity was demonstrated in null-cell ALL, followed, in order, by juvenile T-ALL, adult pleomorphic T-cell leukemia, and AML. Other kinds of lymphoid and monocytic leukemias exhibited low GC receptor and no TdT activity. Although low GC receptor and negative TdT were demonstrated in cells from seven out of nine patients under CML blastic crisis, the last patient had cells with positive TdT and GC receptor activity.

Adolescent↗
PubMed1981

[Studies on specific markers of hormone-dependence in endometrial carcinoma : estradiol receptors and uterine peroxidase (author's transl)].

Both cytosol estradiol-17 beta (E2) receptors and uterine peroxidase were detected in normal uterine tissues of rat and hamster, however, they were not detected when the tissues were cultivated in vitro. Uterine peroxidase was specifically induced by the administration of E2 in these normal uterine tissues in vivo. On the other hand, malignant tissues formed by the transplantation of uterine and mammary adenocarcinoma cells showed hormone-independence. Neither cytosol E2 receptors nor peroxidase was detected in these malignant tissues. These results indicate that, in addition of E2 receptors, peroxidase induction by E2 is a specific marker for hormone-dependence in both normal and abnormal uterine tissues.

Animals↗
PubMed1981

[Study on uterine peroxidase as a specific marker of estrogen-dependence in rat (author's transl)].

Uterine Peroxidase (UP) can be induced by the administration of estradiol (E2) in the rat. We studied whether this biochemical parameter can be a specific marker of estrogen-dependence of rat uterus. 1. UP content in both immature castrated mature rat uteri was negligible. Whereas UP could be detected after vaginal openning of intact rats. 2. UP induction by E2 administration was first detected at 15-day-old rat uteri. 3. UP induction by E2 or mare serum gonadotropin was detected in both immature and castrated mature rats with the increase of uterine weight. 4. UP induction by E2 was observed only in estrogen-dependent tissues, such as uterus and vagina. 5. UP induction by E2 was prominently suppressed by the treatment with actinomycin-D, cycloheximide and tamoxifen. These results suggest that UP induction by E2 is an useful specific marker when hormone-dependence of rat uterus is investigated.

Aging↗
PubMed1980

T lymphocytes expressing human Ia-like antigens in infectious mononucleosis (IM).

In six patients with Epstein-Barr virus (EBV) induced infectious mononucleosis (IM) and in two patients with IM-like syndrome, peripheral blood lymphocytes in the acute and convalescent phase were tested for human la-like antigens as well as other cell surface markers. The major population of T lymphocytes in the acute phase showed la-like antigens, as detected by indirect immunofluorescence with heteroantisera, and the number of la-like antigen-positive T lymphocytes decreased with convalescence. The crossabsorption study indicated that the amount of la-like antigen on the surface of IM T cells was less than that of Raji cells.

Adult↗
PubMed1979

14q translocations, having a break point at 14q13, in lymphoid malignancy.

In three patients with non-Hodgkin lymphomas and in one cell line (HPL-Hod) derived from pleural effusion cells of a patient with Hodgkin's disease, rearrangements of the long arm of chromosome No. 14(14q) were observed. These rearrangements appeared to be consistently associated with a 14q translocation, suggestive of occurrence of a break at 14q13. The translocation in an individual case could occur with 1p, 2q, 4q, and another 14q. A 14q13 translocation may be comparable with a 14q32 translocation, which has often been observed in various types of lymphoid malignancy.

Adult↗
PubMed1979

Cyclophosphamide eliminates suppressor T cells in age-associated central regulation of delayed hypersensitivity in mice.

Effect of treatment of mice with cyclophosphamide (CY) on the delayed hypersensitivity (DH) response was investigated in C57BL/6 mice. DH to methylated human serum albumin (MHSA) could be enhanced with CY in young mice but not in aged ones. DH enhancement with CY appeared to be due to elimination of suppressor T cells involved in DH. Effector T cells were also sensitive to CY, the damaging effect of CY on these latter cells was, however, transient suggesting the rapid recovery of effector T cells. The overshooting recovery of the effector T cells required the presence of the thymus. It is more probably that there are at least two distinct subpopulations of T cells in DH, effector T cells, and suppressor T cells. The distinction is already apparent in the thymus stage. The suppressor T cells, categorized as a central regulator, seem to be antigen nonspecific and regulate the more effectively the DH in young mice, thus physiological role of these cells in age-associated immune alterations is implicated.

Aging↗