Biomedical subjects

S Miyasaka

Publications and source records attributed to S Miyasaka.

39 records · Page 3Linked to original sources

PubMedDate not supplied

The ABO blood grouping of a minute hair sample by the immunohistochemical technique.

The unlabeled antibody (PAP) immunoperoxidase technique was applied to the ABO blood grouping of human scalp hairs. Hair samples were subjected to longitudinal- or cross-sectioning, thus obtaining suitable samples for subsequent immunostaining. The immunostaining was carried out using rabbit anti-A and anti-B sera as the primary antibodies. With this technique, the group-specific staining which is revealed as a dark brown precipitate was clearly observed within the medullae of the hair shaft, and depending on the presence or absence of these precipitates, respective blood groups of unknown hair samples were determined. At the hair root, on the other hand, positive stainings were observed not only in medullary cells but also in some cortical cells of the keratogenous zone. From the present study, it can be safely said that this technique is of practical use for the ABO blood grouping from a minute (less than 3 mm) hair sample.

ABO Blood-Group System↗

PubMedDate not supplied

Prognosis of endogenous fungal endophthalmitis and utility of Ishibashi's classification.

PURPOSE: Evaluations of visual acuity outcomes of eyes with endogenous fungal endophthalmitis were made retrospectively, according to the classification proposed by Ishibashi. METHODS: We surveyed endogenous fungal endophthalmitis cases at the 4 Nihon University Hospitals and 20 affiliated hospitals. Sixty eyes of 34 patients were classified into five stages according to Ishibashi's system, and therapeutic methods and visual outcomes in each stage were then evaluated. RESULTS: Systemic antifungal drugs were efficacious in 82% of stage II and 69% of stage IIIa cases. Antifungal drugs were even efficacious in 42% of stage IIIb cases. Among the unresponsive cases, only half had been given the maximal dosage of antifungal drugs. Half of the eyes in which vitrectomy had been performed at stage IIIb achieved a postoperative visual acuity of 0.5 or better and none had a visual acuity of less than 0.03. CONCLUSION: Based on the above results, we concluded that systemic antifungal drugs should be administered at the maximal dosage to stage II and IIIa cases. If these eyes progress to stage IIIb despite receiving the maximal dosage, vitrectomy is indicated. For stage IIIb eyes, the maximal dosage should be administered first. If not efficacious, vitrectomy should be carried out before progression to stage IV.

Adolescent↗

PubMedDate not supplied

Quantitative analysis of amplifiable DNA in tissues exposed to various environments using competitive PCR assays.

Competitive PCR assays were established for the mitochondrial DNA hypervariable region I and the human amelogenin locus. Using these assays, the copy numbers of DNA participating in PCR (amplifiable DNA) were quantified in tissues exposed to different environments. Human ribs, skin and nails were left in three exposure conditions (in the open air, in soil and in water). The amounts of amplifiable DNA in these tissues were quantified during a time period of up to two months. The amount of amplifiable DNA was well preserved in hard tissues (ribs and nails) regardless of the exposure conditions, whereas the soft tissues immersed in water showed a rapid decrease in amplifiable DNA. Strong PCR inhibition was observed in the DNA extracts obtained from buried bones. This phenomenon was clearly identified from an amplification failure of the internal standards in the competitive PCR. A preliminary examination to identify the PCR inhibitor suggested that the soil itself contributed to the inhibition. In addition, the amounts of amplifiable DNA in case samples were also investigated.

DNA↗