Search PubMed⌕ Search

Biomedical subjects

S Miyake

Publications and source records attributed to S Miyake.

At least 289 records · Page 16Linked to original sources
PubMed1987

[A case of successful surgical treatment of rectal cancer complicated by acute myelomonocytic leukemia].

A 47-year-old man, who had been diagnosed as having acute myelomonocytic leukemia (AMMOL) and had been treated with combination chemotherapy, was admitted to our hospital because he had developed melena. He had been judged to be in complete remission and had shown no signs of recurrence for years, Daunorubicin, vincristine 6-Mercaptopurine and Cyclophosphamide had been administered for maintenance and intensification therapy. He was well until January 1986, when this melena began. A barium enema was given and he was diagnosed as having rectal cancer. Amputation of the rectum and a permanent abdominal colostomy was made safely, mainly because he had been in complete remission, and he recovered normally after the operation. In recent years, the survival of patients with malignancies has improved due to aggressive treatment even in cases of hematological neoplasms. However, the risk of secondary neoplasms in patients treated for cancer has increased. This case suggests that we have to be careful when prescribing treatment for cancer patients, since anti-tumor drugs may have cartinogenic effects.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1986

Effects of listening to music with headphones on hearing--especially under noisy conditions.

The purpose of this experiment was to clarify the effects of exposure to music using headphones under noisy conditions on hearing. The most comfortable loudness (MCL) for three kinds of music (Rock, Popular, Japanese songs) decided by two normal hearing subjects was measured under 6 noisy conditions (Train, Subway, Tram, Bus, Underground, Street) in a soundproof room. In the same manner, the MCL of favorite tunes of five subjects were measured. Temporary threshold shift 2 min after exposure (TTS2) to music for 30 min at the highest MCL was obtained. Furthermore, the characteristics such as spectral structures in one-third octave band or level fluctuations (coefficient of variation) were obtained for noise and music and compared. Statistical analysis revealed that MCL in Street was significantly higher than under other conditions and there was no significant differences in MCL among the various types of music. However, the highest MCL was found for Rock. About 20 dB of TTS was observed in one ear and the hazardous of headphones use in noisy conditions was suggested.

Adult↗
PubMed1986

Gene organization of the small subunit of human calcium-activated neutral protease.

The gene for the small subunit of human calcium-activated neutral protease was isolated and sequenced. It is 11 kb long and comprises 11 exons. No TATA or CAT box was found upstream of the possible transcription initiation sites, but there are three so-called G-C box sequences and one G-C box-like sequence, which are usually found in "house-keeping" genes. The first exon (exon 1) contains only the 5'-noncoding sequence and exon 2 encodes the Gly-rich hydrophobic domain. Each of the four calcium-binding loop regions is encoded by one exon (exons 7-10). The intron breakpoints in the C-terminal calcium-binding domain (exons 4-11) completely coincide with those of the chicken large subunit gene. These findings suggest that the small and large subunits have evolved from the same ancestral calcium-binding protein and have retained the original gene organization.

Amino Acid Sequence↗
PubMed1986

A new method of detection of islet cell antibodies (ICA) using peroxidase-labeled protein A, and incidence of ICA in type 1 (insulin-dependent) diabetes.

We have developed a new method of detecting islet cell antibodies using peroxidase-labeled protein A, and have determined the incidence of ICA in Type 1 (insulin-dependent) diabetes in Japan. In our method, fresh frozen sections of human pancreas and serum samples were incubated and then treated with peroxidase-labeled protein A at room temperature. Conjugates of peroxidase and protein A were subjected to Sephadex G-200 column chromatography, and only the 80,000 dalton peak was employed. The treated sections were allowed to react with haematoxylin and eosin (HE) to confirm the localization of islet cells. With this method, human pancreatic tissues can be used regardless of age and blood type, and the stained sections can be stored for more than 5 years. Serum samples obtained from 52 patients with Type 1 diabetes, 54 with Type 2 (non-insulin-dependent) diabetes and 100 control subjects were examined. In patients with Type 1 diabetes, islet cell antibodies were detected in 14 of 14 (0.5 years after onset), 3 of 6 (0.5-1 years after onset), 7 of 16 (1-5 years after onset) and 2 of 16 (more than 5 years after onset). In contrast, only 4 of 54 patients with Type 2 diabetes and none of the controls were ICA positive. It is concluded that, with our newly developed method using peroxidase-labeled protein A, ICA is present in all Japanese Type 1 diabetic patients whose diabetic manifestations are less than 0.5 years duration from onset.

Adult↗
PubMed1986

Rearrangement of immunoglobulin heavy chain genes and T3 expression in the absence of rearrangement of T-cell receptor beta-chain gene in a patient with T-cell malignant lymphoma.

We describe the rearrangement of immunoglobulin genes and T3 expression in the absence of rearrangement of T-cell receptor beta-chain genes in a patient with T-cell malignant lymphoma. He had a mediastinal mass and his lymphoma cells expressed T-cell antigens (OKT3+, OKT9+, and OKT10+). When we examined genomic DNA from the lymphoma cells, we detected the rearrangement of immunoglobulin heavy chain genes with a germ-line configuration of light chain genes and no rearrangement of T-cell receptor beta-chain gene. These results indicated that the rearrangement of immunoglobulin genes could occur in T-cell malignant lymphoma, and that T3 antigen could be expressed prior to the rearrangement of T-cell receptor beta-chain genes under certain circumstances.

Adult↗
PubMed1986

Structure and function of the small (30K) subunit of calcium-activated neutral protease (CANP).

Calcium-activated neutral protease (CANP), a typical intracellular protease, is composed of a catalytic 80K subunit (80K) and a 30K subunit (30K) of unknown function. The structure of rabbit CANP 30K was examined to clarify its role in the enzyme function. It has a clear two-domain structure composed of 266 amino acid residues. The N-terminal domain presumably determines the location of CANP, whereas the C-terminal domain is a calmodulin-like Ca2+-binding domain and regulates CANP activity.

Amino Acid Sequence↗